Restriction map for
pFN21AB7278
(Linear) (Six-base) MAPSORT of: KIBB7278.seq Check: 6355 from: 1 to: 2067
KIBB7278.orf
With 182 enzymes: SgfI *
April 24, 2009 13:07 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 103 2067
Size: 103 1964
AccI GT'mk_AC
Cuts at: 0 184 221 2067
Size: 184 37 1846
Fragments arranged by size:
1846 184 37
AclI AA'CG_TT
Cuts at: 0 486 2067
Size: 486 1581
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 2007 2067
Size: 2007 60
AfeI AGC'GCT
Cuts at: 0 552 2067
Size: 552 1515
AgeI A'CCGG_T
Cuts at: 0 1287 2067
Size: 1287 780
AleI CACnn'nnGTG
Cuts at: 0 1384 2067
Size: 1384 683
AlwNI CAG_nnn'CTG
Cuts at: 0 827 1557 2067
Size: 827 730 510
Fragments arranged by size:
827 730 510
ApaI G_GGCC'C
Cuts at: 0 1330 1600 1799 2067
Size: 1330 270 199 268
Fragments arranged by size:
1330 270 268 199
ApaLI G'TGCA_C
Cuts at: 0 1047 2067
Size: 1047 1020
ApoI r'AATT_y
Cuts at: 0 943 2067
Size: 943 1124
AvaI C'yCGr_G
Cuts at: 0 666 812 1071 1126 1345 1567 2067
Size: 666 146 259 55 219 222 500
Fragments arranged by size:
666 500 259 222 219 146 55
BanI G'GyrC_C
Cuts at: 0 592 654 1294 1386 1435 1471 2067
Size: 592 62 640 92 49 36 596
Fragments arranged by size:
640 596 592 92 62 49 36
BanII G_rGCy'C
Cuts at: 0 1330 1600 1720 1756 1799 1967 2067
Size: 1330 270 120 36 43 168 100
Fragments arranged by size:
1330 270 168 120 100 43 36
BbeI G_GCGC'C
Cuts at: 0 1298 1439 1475 2067
Size: 1298 141 36 592
Fragments arranged by size:
1298 592 141 36
BbvCI CC'TCA_GC
Cuts at: 0 228 847 2067
Size: 228 619 1220
Fragments arranged by size:
1220 619 228
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 1065 1099 1137 1171 2067
Size: 1065 34 38 34 896
Fragments arranged by size:
1065 896 38 34 34
BglI GCCn_nnn'nGGC
Cuts at: 0 1183 1341 2067
Size: 1183 158 726
Fragments arranged by size:
1183 726 158
Bme1580I G_kGCm'C
Cuts at: 0 595 1051 1330 1600 1799 2005 2067
Size: 595 456 279 270 199 206 62
Fragments arranged by size:
595 456 279 270 206 199 62
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1630 2028 2067
Size: 1630 398 39
Fragments arranged by size:
1630 398 39
Bpu10I CC'TnA_GC
Cuts at: 0 136 228 847 1709 2067
Size: 136 92 619 862 358
Fragments arranged by size:
862 619 358 136 92
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 163 1981 2067
Size: 163 1818 86
Fragments arranged by size:
1818 163 86
BsaI GGTCTCn'nnnn_
Cuts at: 0 259 2067
Size: 259 1808
BsaHI Gr'CG_yC
Cuts at: 0 1295 1436 1472 2067
Size: 1295 141 36 595
Fragments arranged by size:
1295 595 141 36
BsaWI w'CCGG_w
Cuts at: 0 1287 2067
Size: 1287 780
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 2026 2056 2067
Size: 2026 30 11
Fragments arranged by size:
2026 30 11
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 385 967 1213 1254 2002 2067
Size: 385 582 246 41 748 65
Fragments arranged by size:
748 582 385 246 65 41
BseYI C'CCAG_C
Cuts at: 0 834 1336 1395 1545 1557 2067
Size: 834 502 59 150 12 510
Fragments arranged by size:
834 510 502 150 59 12
BsiEI CG_ry'CG
Cuts at: 0 1104 1280 1790 2067
Size: 1104 176 510 277
Fragments arranged by size:
1104 510 277 176
BsiHKAI G_wGCw'C
Cuts at: 0 1051 1756 2067
Size: 1051 705 311
Fragments arranged by size:
1051 705 311
BsmI GAATG_Cn'
Cuts at: 0 117 481 2067
Size: 117 364 1586
Fragments arranged by size:
1586 364 117
BsmBI CGTCTCn'nnnn_
Cuts at: 0 1123 2067
Size: 1123 944
Bsp1286I G_dGCh'C
Cuts at: 0 595 1051 1330 1600 1720 1756 1799 1967
Size: 595 456 279 270 120 36 43 168
Cuts at: 1967 2005 2067
Size: 38 62
Fragments arranged by size:
595 456 279 270 168 120 62 43
38 36
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 103 2067
Size: 103 1964
BsrBI CCG'CTC
Cuts at: 0 344 1014 1691 2067
Size: 344 670 677 376
Fragments arranged by size:
677 670 376 344
BsrDI GCAATG_nn'
Cuts at: 0 1896 2019 2067
Size: 1896 123 48
Fragments arranged by size:
1896 123 48
BsrFI r'CCGG_y
Cuts at: 0 688 748 1287 1340 1979 2067
Size: 688 60 539 53 639 88
Fragments arranged by size:
688 639 539 88 60 53
BssHII G'CGCG_C
Cuts at: 0 1562 2067
Size: 1562 505
BstAPI GCAn_nnn'nTGC
Cuts at: 0 827 2067
Size: 827 1240
BstXI CCAn_nnnn'nTGG
Cuts at: 0 801 1246 2067
Size: 801 445 821
Fragments arranged by size:
821 801 445
BstYI r'GATC_y
Cuts at: 0 430 1038 2067
Size: 430 608 1029
Fragments arranged by size:
1029 608 430
BtgI C'CryG_G
Cuts at: 0 603 917 1274 1315 1622 1655 1772 1790
Size: 603 314 357 41 307 33 117 18
Cuts at: 1790 2067
Size: 277
Fragments arranged by size:
603 357 314 307 277 117 41 33
18
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 897 1972 2067
Size: 897 1075 95
Fragments arranged by size:
1075 897 95
EaeI y'GGCC_r
Cuts at: 0 281 791 879 1101 1184 1277 1787 2067
Size: 281 510 88 222 83 93 510 280
Fragments arranged by size:
510 510 281 280 222 93 88 83
EagI C'GGCC_G
Cuts at: 0 1101 1277 1787 2067
Size: 1101 176 510 280
Fragments arranged by size:
1101 510 280 176
EarI CTCTTCn'nnn_
Cuts at: 0 290 1088 1114 2067
Size: 290 798 26 953
Fragments arranged by size:
953 798 290 26
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 907 976 1442 2067
Size: 907 69 466 625
Fragments arranged by size:
907 625 466 69
EcoICRI GAG'CTC
Cuts at: 0 1754 2067
Size: 1754 313
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1630 2007 2028 2067
Size: 1630 377 21 39
Fragments arranged by size:
1630 377 39 21
EcoNI CCTnn'n_nnAGG
Cuts at: 0 1195 2067
Size: 1195 872
EcoO109I rG'GnC_Cy
Cuts at: 0 670 1326 1597 1679 1795 1796 2067
Size: 670 656 271 82 116 1 271
Fragments arranged by size:
670 656 271 271 116 82 1
HaeII r_GCGC'y
Cuts at: 0 554 980 1154 1298 1376 1439 1475 1499
Size: 554 426 174 144 78 63 36 24
Cuts at: 1499 1825 1980 2034 2067
Size: 326 155 54 33
Fragments arranged by size:
554 426 326 174 155 144 78 63
54 36 33 24
KasI G'GCGC_C
Cuts at: 0 1294 1435 1471 2067
Size: 1294 141 36 596
Fragments arranged by size:
1294 596 141 36
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 78 1460 1937 2067
Size: 78 1382 477 130
Fragments arranged by size:
1382 477 130 78
MscI TGG'CCA
Cuts at: 0 793 2067
Size: 793 1274
MslI CAynn'nnrTG
Cuts at: 0 1384 2067
Size: 1384 683
MspA1I CmG'CkG
Cuts at: 0 333 530 742 827 854 866 1276 1317
Size: 333 197 212 85 27 12 410 41
Cuts at: 1317 1332 1395 1418 1530 1539 1624 1657 1759
Size: 15 63 23 112 9 85 33 102
Cuts at: 1759 1765 1774 1792 1806 1812 2067
Size: 6 9 18 14 6 255
Fragments arranged by size:
410 333 255 212 197 112 102 85
85 63 41 33 27 23 18 15
14 12 9 9 6 6
NaeI GCC'GGC
Cuts at: 0 690 750 1342 1981 2067
Size: 690 60 592 639 86
Fragments arranged by size:
690 639 592 86 60
NarI GG'CG_CC
Cuts at: 0 1295 1436 1472 2067
Size: 1295 141 36 595
Fragments arranged by size:
1295 595 141 36
NdeI CA'TA_TG
Cuts at: 0 1928 2067
Size: 1928 139
NgoMIV G'CCGG_C
Cuts at: 0 688 748 1340 1979 2067
Size: 688 60 592 639 88
Fragments arranged by size:
688 639 592 88 60
NotI GC'GGCC_GC
Cuts at: 0 1277 1787 2067
Size: 1277 510 280
Fragments arranged by size:
1277 510 280
NspI r_CATG'y
Cuts at: 0 470 2067
Size: 470 1597
PflMI CCAn_nnn'nTGG
Cuts at: 0 789 1557 2067
Size: 789 768 510
Fragments arranged by size:
789 768 510
PfoI T'CCnGG_A
Cuts at: 0 947 2067
Size: 947 1120
PpuMI rG'GwC_Cy
Cuts at: 0 670 1679 2067
Size: 670 1009 388
Fragments arranged by size:
1009 670 388
PspOMI G'GGCC_C
Cuts at: 0 1326 1596 1795 2067
Size: 1326 270 199 272
Fragments arranged by size:
1326 272 270 199
PspXI vC'TCGA_Gb
Cuts at: 0 1345 1567 2067
Size: 1345 222 500
Fragments arranged by size:
1345 500 222
PstI C_TGCA'G
Cuts at: 0 1196 1770 1811 2067
Size: 1196 574 41 256
Fragments arranged by size:
1196 574 256 41
PvuII CAG'CTG
Cuts at: 0 333 827 1395 1530 1806 1812 2067
Size: 333 494 568 135 276 6 255
Fragments arranged by size:
568 494 333 276 255 135 6
SacI G_AGCT'C
Cuts at: 0 1756 2067
Size: 1756 311
SacII CC_GC'GG
Cuts at: 0 1277 1318 1625 1658 1775 1793 2067
Size: 1277 41 307 33 117 18 274
Fragments arranged by size:
1277 307 274 117 41 33 18
SanDI GG'GwC_CC
Cuts at: 0 670 1679 2067
Size: 670 1009 388
Fragments arranged by size:
1009 670 388
SapI GCTCTTCn'nnn_
Cuts at: 0 290 2067
Size: 290 1777
SbfI CC_TGCA'GG
Cuts at: 0 1196 2067
Size: 1196 871
SexAI A'CCwGG_T
Cuts at: 0 1916 2067
Size: 1916 151
SfcI C'TryA_G
Cuts at: 0 1192 1766 1807 2067
Size: 1192 574 41 260
Fragments arranged by size:
1192 574 260 41
SfoI GGC'GCC
Cuts at: 0 1296 1437 1473 2067
Size: 1296 141 36 594
Fragments arranged by size:
1296 594 141 36
SgrAI Cr'CCGG_yG
Cuts at: 0 748 1287 1979 2067
Size: 748 539 692 88
Fragments arranged by size:
748 692 539 88
SmaI CCC'GGG
Cuts at: 0 668 814 2067
Size: 668 146 1253
Fragments arranged by size:
1253 668 146
SmlI C'TyrA_G
Cuts at: 0 178 1345 1567 1996 2067
Size: 178 1167 222 429 71
Fragments arranged by size:
1167 429 222 178 71
SpeI A'CTAG_T
Cuts at: 0 378 2067
Size: 378 1689
StyI C'CwwG_G
Cuts at: 0 44 596 1031 1967 2067
Size: 44 552 435 936 100
Fragments arranged by size:
936 552 435 100 44
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 694 726 1030 1062 2026 2058 2067
Size: 694 32 304 32 964 32 9
Fragments arranged by size:
964 694 304 32 32 32 9
XbaI T'CTAG_A
Cuts at: 0 427 2067
Size: 427 1640
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 1038 2067
Size: 1038 1029
XhoI C'TCGA_G
Cuts at: 0 1345 1567 2067
Size: 1345 222 500
Fragments arranged by size:
1345 500 222
XmaI C'CCGG_G
Cuts at: 0 666 812 2067
Size: 666 146 1255
Fragments arranged by size:
1255 666 146
Enzymes that do cut:
AarI AccI AclI AcuI AfeI AgeI AleI AlwNI
ApaI ApaLI ApoI AvaI BanI BanII BbeI BbvCI
BcgI BglI Bme1580I BpmI Bpu10I BpuEI BsaI BsaHI
BsaWI BsaXI BseRI BseYI BsiEI BsiHKAI BsmI BsmBI
Bsp1286I BspMI BsrBI BsrDI BsrFI BssHII BstAPI BstXI
BstYI BtgI BtgZI EaeI EagI EarI EciI EcoICRI
Eco57MI EcoNI EcoO109I HaeII KasI MmeI MscI MslI
MspA1I NaeI NarI NdeI NgoMIV NotI NspI PflMI
PfoI PpuMI PspOMI PspXI PstI PvuII SacI SacII
SanDI SapI SbfI SexAI SfcI SfoI SgrAI SmaI
SmlI SpeI StyI TstI XbaI XcmI XhoI XmaI
Enzymes that do not cut:
AatII Acc65I AflII AflIII AhdI AlfI AloI AscI
AseI AsiSI AvrII BaeI BamHI BbsI BciVI BclI
BfrBI BglII BlpI BmgBI BmrI BmtI BplI BsaAI
BsaBI BsgI BsiWI BspEI BspHI BsrGI BssSI BstBI
BstEII BstZ17I Bsu36I BtsI ClaI CspCI DraI DraIII
DrdI EcoRI EcoRV FalI FseI FspI FspAI Hin4I
HincII HindIII HpaI KpnI MfeI MluI NcoI NheI
NruI NsiI PacI PasI PciI PmeI PmlI PpiI
PshAI PsiI PsrI PvuI RsrII SalI ScaI SfiI
SgfI SnaBI SphI SrfI SspI StuI SwaI TaqII
TatI Tth111I XmnI ZraI