Restriction map for
pFN21AB8985
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# NO CUT ENZYMES ( 120 enzymes) #
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Acc65I AccI AclI AcsI AflII
AgeI AhdI AlwNI AocI Aor51HI
ApaI ApoI AscI AseI AsnI
Asp700I Asp718I AspEI AspI AvrII
BalI BamHI BbrPI BbsI BbvCI
BcgI BclI BfrI BglII BlnI
BpuAI BsaAI BsaBI BsiWI BsmBI
BsmFI Bsp1407I BspDI BspLU11I BsrDI
BsrGI BssSI Bst1107I BstBI BstZ17I
Bsu36I ClaI CpoI DraI DraII
DraIII Eam1105I Eco47III Eco81I EcoNI
EcoO109I EcoRI EcoRV EcoT14I EcoT22I
FbaI FseI HgaI HindIII HpaI
KpnI KspI MamI MfeI MluNI
MscI MseI MslI MspA1I MunI
NcoI NdeI NheI NotI NruI
NsiI NspV PacI PaeR7I PflFI
PinAI PmaCI PmeI PmlI PpuMI
PshBI Psp1406I PstI PvuI PvuII
RsrII SacI SacII SalI SapI
SbfI ScaI SexAI SfiI SfuI
SgrAI SnaBI SpeI Sse8387I SspBI
SspI StuI StyI SwaI Tru9I
Tsp509I Tth111I XbaI XhoI XmnI
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# ONE CUT ENZYMES ( 49 enzymes) #
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AatII 369 GACGTC GACGT^C
AccIII 618 TCCGGA T^CCGGA
AflIII 824 ACGCGT A^C[GA][CT]GT
Alw44I 1100 GTGCAC G^TGCAC
ApaLI 1100 GTGCAC G^TGCAC
AvaII 645 GGACC G^G[AT]CC
AviII 887 TGCGCA TGC^GCA
BbeI 1119 GGCGCC GGCGC^C
BciVI 420 GTATCC GTATCC(6/5)
BfaI 794 CTAG C^TAG
BlpI 357 GCTCAGC GC^T[ACGT]AGC
BpmI 682 CTGGAG CTGGAG(16/14)
Bpu1102I 357 GCTCAGC GC^T[ACGT]AGC
BsaI 685 GAGACC GGTCTC(1/5)
BsaWI 618 TCCGGA [AT]^CCGG[AT]
BseAI 618 TCCGGA T^CCGGA
BseRI 79 GAGGAG GAGGAG(10/8)
BsgI 388 GTGCAG GTGCAG(16/14)
BsmAI 685 GAGAC GTCTC(1/5)
BsmI 697 GCATTC GAATGC(1/-1)
BspEI 618 TCCGGA T^CCGGA
BspHI 314 TCATGA T^CATGA
BstAPI 1102 GCACCCTCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstXI 599 CCAACGACATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
CelII 357 GCTCAGC GC^T[ACGT]AGC
DrdI 121 GACTCGGTAGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EagI 833 CGGCCG C^GGCCG
EclXI 833 CGGCCG C^GGCCG
Eco52I 833 CGGCCG C^GGCCG
FspI 887 TGCGCA TGC^GCA
HincII 658 GTCAAC GT[CT]^[GA]AC
HindII 658 GTCAAC GT[CT]^[GA]AC
KasI 1119 GGCGCC G^GCGCC
MaeI 794 CTAG C^TAG
MluI 824 ACGCGT A^CGCGT
MroI 618 TCCGGA T^CCGGA
NarI 1119 GGCGCC GG^CGCC
NspI 929 GCATGC [GA]CATG^[CT]
PflMI 636 CCAGGGCATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PshAI 166 GACATCGGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
RcaI 314 TCATGA T^CATGA
SfcI 149 CTACAG C^T[GA][CT]AG
SfoI 1119 GGCGCC GGC^GCC
SphI 929 GCATGC GCATG^C
TaqI 272 TCGA T^CGA
TfiI 570 GATTC G^A[AT]TC
TspRI 916 CAGTG CA[GC]TG[ACGT][ACGT]^
TthHB8I 272 TCGA T^CGA
Van91I 636 CCAGGGCATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
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# TWO CUT ENZYMES ( 28 enzymes) #
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AcyI 369 GACGTC G[GA]^CG[CT]C
1119 GGCGCC
AfaI 72 GTAC GT^AC
780 GTAC
AspHI 622 GAGCAC G[AT]GC[AT]^C
1100 GTGCAC
BglI 535 GCCAAGCCGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
762 GCCCATCCGGC
BsaHI 369 GACGTC G[GA]^CG[CT]C
1119 GGCGCC
BsiEI 833 CGGCCG CG[GA][CT]^CG
846 CGACCG
BsiHKAI 622 GAGCAC G[AT]GC[AT]^C
1100 GTGCAC
BspMI 884 ACCTGC ACCTGC(4/8)
1153 ACCTGC
BsrBI 748 CCGCTC CCGCTC(-3/-3)
805 CCGCTC
BsrI 287 ACTGG ACTGG(1/-1)
915 CCAGT
BssHII 92 GCGCGC G^CGCGC
1027 GCGCGC
BstEII 30 GGTGACC G^GT[ACGT]ACC
1146 GGTCACC
BstPI 30 GGTGACC G^GT[ACGT]ACC
1146 GGTCACC
DdeI 358 CTCAG C^T[ACGT]AG
462 CTCAG
DsaI 374 CCACGG C^C[GA][CT]GG
1142 CCGTGG
EarI 103 CTCTTC CTCTTC(1/4)
559 CTCTTC
EcoO65I 30 GGTGACC G^GT[ACGT]ACC
1146 GGTCACC
Hin1I 369 GACGTC G[GA]^CG[CT]C
1119 GGCGCC
Ksp632I 103 CTCTTC CTCTTC(1/4)
559 CTCTTC
MaeII 370 ACGT A^CGT
407 ACGT
NaeI 322 GCCGGC GCC^GGC
540 GCCGGC
NgoMIV 322 GCCGGC G^CCGGC
540 GCCGGC
PleI 121 GACTC GAGTC(4/5)
274 GACTC
RsaI 72 GTAC GT^AC
780 GTAC
SmaI 499 CCCGGG CCC^GGG
720 CCCGGG
SmlI 757 CTCAAG C^T[CT][GA]AG
1066 CTCAAG
XmaCI 499 CCCGGG C^CCGGG
720 CCCGGG
XmaI 499 CCCGGG C^CCGGG
720 CCCGGG
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# 3 OR MORE CUT ENZYMES ( 61 enzymes) #
#########################################
AccII AciI AluI AlwI AvaI
BanI BanII BbvI BcnI BmyI
BsaJI BsiYI BslI BsoBI Bsp1286I
BsrFI BssKI BstF5I BstNI BstUI
BstYI Cac8I CfoI Cfr10I Cfr13I
DpnII EaeI EcoRII FauI Fnu4HI
FokI HaeII HaeIII HapII HhaI
HinP1I HinfI HpaII HphI ItaI
MaeIII MboI MboII MflI MnlI
MspI MvaI MvnI MwoI NciI
NdeII NlaIII NlaIV Sau3AI Sau96I
ScrFI SfaNI TseI Tsp45I XcmI
XhoII