(Circular) (Six-base) MAPSORT of: pFN21AB8985.seq Check: 3916 from: 1 to: 5866 pFN21AB8985 5866 bp With 182 enzymes: SgfI * October 20, 2009 11:01 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2898 3167 2898 Size: 269 5597 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 2378 3676 278 Size: 53 83 186 936 842 1298 2468 Fragments arranged by size: 2468 1298 936 842 186 83 53 AccI GT'mk_AC Cuts at: 1937 3209 1937 Size: 1272 4594 Acc65I G'GTAC_C Cuts at: 3187 3187 Size: 5866 AclI AA'CG_TT Cuts at: 1563 3993 4366 1563 Size: 2430 373 3063 Fragments arranged by size: 3063 2430 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2337 2929 3929 5313 5581 1632 Size: 705 592 1000 1384 268 1917 Fragments arranged by size: 1917 1384 1000 705 592 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5669 AflIII A'CryG_T Cuts at: 1203 2829 4765 5742 1203 Size: 1626 1936 977 1327 Fragments arranged by size: 1936 1626 1327 977 AgeI A'CCGG_T Cuts at: 4697 4697 Size: 5866 AhdI GACnn_n'nnGTC Cuts at: 4595 4595 Size: 5866 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 5866 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 5866 AlwNI CAG_nnn'CTG Cuts at: 3609 5181 3609 Size: 1572 4294 ApaLI G'TGCA_C Cuts at: 3105 3923 5079 3105 Size: 818 1156 3892 Fragments arranged by size: 3892 1156 818 ApoI r'AATT_y Cuts at: 1493 3175 3401 3553 5662 1493 Size: 1682 226 152 2109 1697 Fragments arranged by size: 2109 1697 1682 226 152 AseI AT'TA_AT Cuts at: 160 1032 4420 160 Size: 872 3388 1606 Fragments arranged by size: 3388 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5866 AvaI C'yCGr_G Cuts at: 1870 1943 2081 2504 2725 3191 1870 Size: 73 138 423 221 466 4545 Fragments arranged by size: 4545 466 423 221 138 73 BamHI G'GATC_C Cuts at: 3196 3563 3196 Size: 367 5499 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2295 2341 2886 3124 3187 Size: 325 217 627 508 46 545 238 63 Cuts at: 3187 4642 618 Size: 1455 1842 Fragments arranged by size: 1842 1455 627 545 508 325 238 217 63 46 BanII G_rGCy'C Cuts at: 1391 2475 2511 2618 3185 1391 Size: 1084 36 107 567 4072 Fragments arranged by size: 4072 1084 567 107 36 BbeI G_GCGC'C Cuts at: 3128 3128 Size: 5866 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 4895 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4057 4091 1903 Size: 34 2120 34 3678 Fragments arranged by size: 3678 2120 34 34 BciVI GTATCCnnnnn_n' Cuts at: 2436 3758 4968 2436 Size: 1322 1210 3334 Fragments arranged by size: 3334 1322 1210 BclI T'GATC_A Cuts at: 1548 1548 Size: 5866 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2546 2773 4477 Size: 107 122 71 631 605 874 227 1704 Cuts at: 4477 136 Size: 1525 Fragments arranged by size: 1704 1525 874 631 605 227 122 107 71 BglII A'GATC_T Cuts at: 5861 5861 Size: 5866 BlpI GC'TnA_GC Cuts at: 2363 3283 2363 Size: 920 4946 Bme1580I G_kGCm'C Cuts at: 1790 2346 2837 3109 3927 5083 1790 Size: 556 491 272 818 1156 2573 Fragments arranged by size: 2573 1156 818 556 491 272 BmgBI CAC'GTC Cuts at: 1318 2413 1318 Size: 1095 4771 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3597 4555 442 Size: 416 53 83 399 24 2180 958 1753 Fragments arranged by size: 2180 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5866 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2708 4526 1239 Size: 1469 1818 2579 Fragments arranged by size: 2579 1818 1469 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2747 3056 3991 4856 5154 2747 Size: 309 935 865 298 3459 Fragments arranged by size: 3459 935 865 309 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2684 4529 882 Size: 617 1185 1845 2219 Fragments arranged by size: 2219 1845 1185 617 BsaAI yAC'GTr Cuts at: 493 1204 3657 493 Size: 711 2453 2702 Fragments arranged by size: 2702 2453 711 BsaBI GATnn'nnATC Cuts at: 5860 5860 Size: 5866 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2375 3125 3673 4055 Size: 53 83 186 936 842 750 548 382 Cuts at: 4055 4690 275 Size: 635 1451 Fragments arranged by size: 1451 936 842 750 635 548 382 186 83 53 BsaWI w'CCGG_w Cuts at: 2623 4299 4697 4971 5118 5529 2623 Size: 1676 398 274 147 411 2960 Fragments arranged by size: 2960 1676 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2096 2126 2204 2234 2408 Size: 30 165 30 693 30 78 30 174 Cuts at: 2408 2438 2600 2630 1178 Size: 30 162 30 4414 Fragments arranged by size: 4414 693 174 165 162 78 30 30 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2099 1186 Size: 913 4953 BseYI C'CCAG_C Cuts at: 2093 2745 5069 5565 2093 Size: 652 2324 496 2394 Fragments arranged by size: 2394 2324 652 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2414 1163 Size: 573 678 4615 Fragments arranged by size: 4615 678 573 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2841 2854 3316 4077 5105 1540 Size: 275 186 840 13 462 761 1028 2301 Fragments arranged by size: 2301 1028 840 761 462 275 186 13 BsiHKAI G_wGCw'C Cuts at: 2631 3109 3927 4012 5083 2631 Size: 478 818 85 1071 3414 Fragments arranged by size: 3414 1071 818 478 85 BsmI GAATG_Cn' Cuts at: 2702 3377 3470 2702 Size: 675 93 5098 Fragments arranged by size: 5098 675 93 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2346 2475 2511 2618 2631 2837 3109 Size: 399 556 129 36 107 13 206 272 Cuts at: 3109 3185 3927 4012 5083 1391 Size: 76 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 556 399 272 206 129 107 85 76 36 13 BspEI T'CCGG_A Cuts at: 2623 5529 2623 Size: 2906 2960 BspHI T'CATG_A Cuts at: 2319 3755 5485 2319 Size: 1436 1730 2700 Fragments arranged by size: 2700 1730 1436 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2898 3167 3221 844 Size: 2054 269 54 3489 Fragments arranged by size: 3489 2054 269 54 BsrBI CCG'CTC Cuts at: 1394 1823 2755 2812 3753 1394 Size: 429 932 57 941 3507 Fragments arranged by size: 3507 941 932 429 57 BsrDI GCAATG_nn' Cuts at: 65 1248 4361 4535 65 Size: 1183 3113 174 1396 Fragments arranged by size: 3113 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2009 2032 2327 2545 2793 4510 4697 Size: 110 199 23 295 218 248 1717 187 Cuts at: 4697 1700 Size: 2869 Fragments arranged by size: 2869 1717 295 248 218 199 187 110 23 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5866 BssHII G'CGCG_C Cuts at: 1925 2097 3032 1925 Size: 172 935 4759 Fragments arranged by size: 4759 935 172 BssSI C'ACGA_G Cuts at: 3926 4938 3926 Size: 1012 4854 BstAPI GCAn_nnn'nTGC Cuts at: 794 3113 794 Size: 2319 3547 BstBI TT'CG_AA Cuts at: 4670 4670 Size: 5866 BstEII G'GTnAC_C Cuts at: 1184 2035 3151 1184 Size: 851 1116 3899 Fragments arranged by size: 3899 1116 851 BstXI CCAn_nnnn'nTGG Cuts at: 2611 2611 Size: 5866 BstYI r'GATC_y Cuts at: 1972 2088 2718 2928 3063 3196 3563 3948 3965 Size: 116 630 210 135 133 367 385 17 Cuts at: 3965 5417 5503 5515 5861 1972 Size: 1452 86 12 346 1977 Fragments arranged by size: 1977 1452 630 385 367 346 210 135 133 116 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2379 3147 513 Size: 552 1314 768 3232 Fragments arranged by size: 3232 1314 768 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5127 Fragments arranged by size: 5127 683 56 BtsI GCAGTG_nn' Cuts at: 3382 4176 4196 3382 Size: 794 20 5052 Fragments arranged by size: 5052 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5866 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5831 DraI TTT'AAA Cuts at: 3170 3170 Size: 5866 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 2132 4873 809 Size: 507 816 2741 1802 Fragments arranged by size: 2741 1802 816 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2382 2538 2838 Size: 54 1425 50 275 18 552 156 300 Cuts at: 2838 3313 4202 5831 8 Size: 475 889 1629 43 Fragments arranged by size: 1629 1425 889 552 475 300 275 156 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 2838 3313 1537 Size: 275 1026 475 4090 Fragments arranged by size: 4090 1026 475 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2114 2570 3796 1353 Size: 761 456 1226 3423 Fragments arranged by size: 3423 1226 761 456 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 3107 4453 4827 4973 387 Size: 1064 138 1518 1346 374 146 1280 Fragments arranged by size: 1518 1346 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2337 2708 2929 3929 4526 5313 5581 Size: 393 705 371 221 1000 597 787 268 Cuts at: 5581 1239 Size: 1524 Fragments arranged by size: 1524 1000 787 705 597 393 371 268 221 EcoRI G'AATT_C Cuts at: 3175 3553 3175 Size: 378 5488 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2290 2290 Size: 5866 FspI TGC'GCA Cuts at: 2894 4372 2894 Size: 1478 4388 HaeII r_GCGC'y Cuts at: 1678 2328 2363 3055 3128 5013 1678 Size: 650 35 692 73 1885 2531 Fragments arranged by size: 2531 1885 692 650 73 35 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2450 2462 2482 2494 2519 Size: 32 135 32 878 12 20 12 25 Cuts at: 2519 2551 2600 2632 4507 4539 4581 4613 1373 Size: 32 49 32 1875 32 42 32 2626 Fragments arranged by size: 2626 1875 878 135 49 42 32 32 32 32 32 32 25 20 12 12 HincII GTy'rAC Cuts at: 669 1657 1938 2665 3210 3456 669 Size: 988 281 727 545 246 3079 Fragments arranged by size: 3079 988 727 545 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 5866 HpaI GTT'AAC Cuts at: 3456 3456 Size: 5866 KasI G'GCGC_C Cuts at: 3124 3124 Size: 5866 KpnI G_GTAC'C Cuts at: 3191 3191 Size: 5866 MfeI C'AATT_G Cuts at: 3465 3465 Size: 5866 MluI A'CGCG_T Cuts at: 2829 5742 2829 Size: 2913 2953 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2313 2472 2532 4980 5164 1305 Size: 1008 159 60 2448 184 2007 Fragments arranged by size: 2448 2007 1008 184 159 60 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 4044 Fragments arranged by size: 4044 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 3824 4183 4342 518 Size: 648 2658 359 159 2042 Fragments arranged by size: 2658 2042 648 359 159 MspA1I CmG'CkG Cuts at: 1606 3282 3959 5107 5352 1606 Size: 1676 677 1148 245 2120 Fragments arranged by size: 2120 1676 1148 677 245 NaeI GCC'GGC Cuts at: 2329 2547 2329 Size: 218 5648 NarI GG'CG_CC Cuts at: 3125 3125 Size: 5866 NcoI C'CATG_G Cuts at: 513 1065 513 Size: 552 5314 NdeI CA'TA_TG Cuts at: 387 387 Size: 5866 NgoMIV G'CCGG_C Cuts at: 2327 2545 2327 Size: 218 5648 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5866 NotI GC'GGCC_GC Cuts at: 3313 3313 Size: 5866 NspI r_CATG'y Cuts at: 1132 1229 2938 3224 4769 1132 Size: 97 1709 286 1545 2229 Fragments arranged by size: 2229 1709 1545 286 97 PasI CC'CwG_GG Cuts at: 1348 2494 1348 Size: 1146 4720 PciI A'CATG_T Cuts at: 4765 4765 Size: 5866 PflMI CCAn_nnn'nTGG Cuts at: 1108 2647 1108 Size: 1539 4327 PmeI GTTT'AAAC Cuts at: 3170 3170 Size: 5866 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3935 3967 5448 5480 3935 Size: 32 1481 32 4321 Fragments arranged by size: 4321 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 2175 1613 Size: 562 5304 PsiI TTA'TAA Cuts at: 3436 3436 Size: 5866 PstI C_TGCA'G Cuts at: 830 3218 830 Size: 2388 3478 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5866 SalI G'TCGA_C Cuts at: 1936 3208 1936 Size: 1272 4594 SbfI CC_TGCA'GG Cuts at: 3218 3218 Size: 5866 ScaI AGT'ACT Cuts at: 4114 5826 4114 Size: 1712 4154 SfcI C'TryA_G Cuts at: 826 1045 2154 3214 4349 5030 5221 826 Size: 219 1109 1060 1135 681 191 1471 Fragments arranged by size: 1471 1135 1109 1060 681 219 191 SfoI GGC'GCC Cuts at: 3126 3126 Size: 5866 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5866 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5866 SmaI CCC'GGG Cuts at: 1872 2506 2727 3193 1872 Size: 634 221 466 4545 Fragments arranged by size: 4545 634 466 221 SmlI C'TyrA_G Cuts at: 820 1017 1943 2762 3071 3970 4871 5133 820 Size: 197 926 819 309 899 901 262 1553 Fragments arranged by size: 1553 926 901 899 819 309 262 197 SnaBI TAC'GTA Cuts at: 493 3657 493 Size: 3164 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5866 SphI G_CATG'C Cuts at: 1132 2938 3224 1132 Size: 1806 286 3774 Fragments arranged by size: 3774 1806 286 SspI AAT'ATT Cuts at: 5 52 3790 5 Size: 47 3738 2081 Fragments arranged by size: 3738 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 5866 StyI C'CwwG_G Cuts at: 513 1065 3305 513 Size: 552 2240 3074 Fragments arranged by size: 3074 2240 552 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2165 3893 3910 4063 4248 4693 325 Size: 1255 585 1728 17 153 185 445 1498 Fragments arranged by size: 1728 1498 1255 585 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 4112 5824 96 Size: 274 80 33 51 1443 2135 1712 138 Fragments arranged by size: 2135 1712 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 5826 5858 5826 Size: 32 5834 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5866 XbaI T'CTAG_A Cuts at: 3202 3202 Size: 5866 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2386 2548 2729 2386 Size: 162 181 5523 Fragments arranged by size: 5523 181 162 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5866 XmaI C'CCGG_G Cuts at: 1870 2504 2725 3191 1870 Size: 634 221 466 4545 Fragments arranged by size: 4545 634 466 221 XmnI GAAnn'nnTTC Cuts at: 3995 3995 Size: 5866 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 2376 3674 276 Size: 53 83 186 936 842 1298 2468 Fragments arranged by size: 2468 1298 936 842 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PmeI PpiI PshAI PsiI PstI PvuI SalI SbfI ScaI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI AloI ApaI AscI AvrII BaeI BbvCI BfrBI BplI Bpu10I BsiWI BsmBI BstZ17I Bsu36I DraIII EcoICRI EcoNI EcoO109I EcoRV FseI FspAI NruI NsiI PacI PfoI PmlI PpuMI PspOMI PspXI PsrI PvuII RsrII SacI SacII SanDI SapI SexAI SfiI SrfI SwaI