Restriction map for
pFN21AB8985
(Linear) (Six-base) MAPSORT of: KIBB8985.seq Check: 1849 from: 1 to: 1161
KIBB8985.orf
With 182 enzymes: SgfI *
April 24, 2009 13:08 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 893 1161
Size: 893 268
AatII G_ACGT'C
Cuts at: 0 373 1161
Size: 373 788
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 332 924 1161
Size: 332 592 237
Fragments arranged by size:
592 332 237
AflIII A'CryG_T
Cuts at: 0 824 1161
Size: 824 337
ApaLI G'TGCA_C
Cuts at: 0 1100 1161
Size: 1100 61
AvaI C'yCGr_G
Cuts at: 0 76 499 720 1161
Size: 76 423 221 441
Fragments arranged by size:
441 423 221 76
BanI G'GyrC_C
Cuts at: 0 290 336 881 1119 1161
Size: 290 46 545 238 42
Fragments arranged by size:
545 290 238 46 42
BanII G_rGCy'C
Cuts at: 0 470 506 613 1161
Size: 470 36 107 548
Fragments arranged by size:
548 470 107 36
BbeI G_GCGC'C
Cuts at: 0 1123 1161
Size: 1123 38
BciVI GTATCCnnnnn_n'
Cuts at: 0 431 1161
Size: 431 730
BglI GCCn_nnn'nGGC
Cuts at: 0 541 768 1161
Size: 541 227 393
Fragments arranged by size:
541 393 227
BlpI GC'TnA_GC
Cuts at: 0 358 1161
Size: 358 803
Bme1580I G_kGCm'C
Cuts at: 0 341 832 1104 1161
Size: 341 491 272 57
Fragments arranged by size:
491 341 272 57
BmgBI CAC'GTC
Cuts at: 0 408 1161
Size: 408 753
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 703 1161
Size: 703 458
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 742 1051 1161
Size: 742 309 110
Fragments arranged by size:
742 309 110
BsaI GGTCTCn'nnnn_
Cuts at: 0 679 1161
Size: 679 482
BsaHI Gr'CG_yC
Cuts at: 0 370 1120 1161
Size: 370 750 41
Fragments arranged by size:
750 370 41
BsaWI w'CCGG_w
Cuts at: 0 618 1161
Size: 618 543
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 91 121 199 229 403 433 595 625
Size: 91 30 78 30 174 30 162 30
Cuts at: 625 1161
Size: 536
Fragments arranged by size:
536 174 162 91 78 30 30 30
30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 94 1161
Size: 94 1067
BseYI C'CCAG_C
Cuts at: 0 88 740 1161
Size: 88 652 421
Fragments arranged by size:
652 421 88
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 409 1161
Size: 409 752
BsiEI CG_ry'CG
Cuts at: 0 836 849 1161
Size: 836 13 312
Fragments arranged by size:
836 312 13
BsiHKAI G_wGCw'C
Cuts at: 0 626 1104 1161
Size: 626 478 57
Fragments arranged by size:
626 478 57
BsmI GAATG_Cn'
Cuts at: 0 697 1161
Size: 697 464
Bsp1286I G_dGCh'C
Cuts at: 0 341 470 506 613 626 832 1104 1161
Size: 341 129 36 107 13 206 272 57
Fragments arranged by size:
341 272 206 129 107 57 36 13
BspEI T'CCGG_A
Cuts at: 0 618 1161
Size: 618 543
BspHI T'CATG_A
Cuts at: 0 314 1161
Size: 314 847
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 893 1161
Size: 893 268
BsrBI CCG'CTC
Cuts at: 0 750 807 1161
Size: 750 57 354
Fragments arranged by size:
750 354 57
BsrFI r'CCGG_y
Cuts at: 0 4 27 322 540 788 1161
Size: 4 23 295 218 248 373
Fragments arranged by size:
373 295 248 218 23 4
BssHII G'CGCG_C
Cuts at: 0 92 1027 1161
Size: 92 935 134
Fragments arranged by size:
935 134 92
BstAPI GCAn_nnn'nTGC
Cuts at: 0 1108 1161
Size: 1108 53
BstEII G'GTnAC_C
Cuts at: 0 30 1146 1161
Size: 30 1116 15
Fragments arranged by size:
1116 30 15
BstXI CCAn_nnnn'nTGG
Cuts at: 0 606 1161
Size: 606 555
BstYI r'GATC_y
Cuts at: 0 83 713 923 1058 1161
Size: 83 630 210 135 103
Fragments arranged by size:
630 210 135 103 83
BtgI C'CryG_G
Cuts at: 0 374 1142 1161
Size: 374 768 19
Fragments arranged by size:
768 374 19
DrdI GACnn_nn'nnGTC
Cuts at: 0 127 1161
Size: 127 1034
EaeI y'GGCC_r
Cuts at: 0 377 533 833 1161
Size: 377 156 300 328
Fragments arranged by size:
377 328 300 156
EagI C'GGCC_G
Cuts at: 0 833 1161
Size: 833 328
EarI CTCTTCn'nnn_
Cuts at: 0 109 565 1161
Size: 109 456 596
Fragments arranged by size:
596 456 109
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 1102 1161
Size: 1102 59
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 332 703 924 1161
Size: 332 371 221 237
Fragments arranged by size:
371 332 237 221
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 285 1161
Size: 285 876
FspI TGC'GCA
Cuts at: 0 889 1161
Size: 889 272
HaeII r_GCGC'y
Cuts at: 0 323 358 1050 1123 1161
Size: 323 35 692 73 38
Fragments arranged by size:
692 323 73 38 35
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 445 457 477 489 514 546 595 627
Size: 445 12 20 12 25 32 49 32
Cuts at: 627 1161
Size: 534
Fragments arranged by size:
534 445 49 32 32 25 20 12
12
HincII GTy'rAC
Cuts at: 0 660 1161
Size: 660 501
KasI G'GCGC_C
Cuts at: 0 1119 1161
Size: 1119 42
MluI A'CGCG_T
Cuts at: 0 824 1161
Size: 824 337
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 308 467 527 1161
Size: 308 159 60 634
Fragments arranged by size:
634 308 159 60
NaeI GCC'GGC
Cuts at: 0 324 542 1161
Size: 324 218 619
Fragments arranged by size:
619 324 218
NarI GG'CG_CC
Cuts at: 0 1120 1161
Size: 1120 41
NgoMIV G'CCGG_C
Cuts at: 0 322 540 1161
Size: 322 218 621
Fragments arranged by size:
621 322 218
NspI r_CATG'y
Cuts at: 0 933 1161
Size: 933 228
PasI CC'CwG_GG
Cuts at: 0 489 1161
Size: 489 672
PflMI CCAn_nnn'nTGG
Cuts at: 0 642 1161
Size: 642 519
PshAI GACnn'nnGTC
Cuts at: 0 170 1161
Size: 170 991
SfcI C'TryA_G
Cuts at: 0 149 1161
Size: 149 1012
SfoI GGC'GCC
Cuts at: 0 1121 1161
Size: 1121 40
SmaI CCC'GGG
Cuts at: 0 501 722 1161
Size: 501 221 439
Fragments arranged by size:
501 439 221
SmlI C'TyrA_G
Cuts at: 0 757 1066 1161
Size: 757 309 95
Fragments arranged by size:
757 309 95
SphI G_CATG'C
Cuts at: 0 933 1161
Size: 933 228
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 160 1161
Size: 160 1001
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 381 543 724 1161
Size: 381 162 181 437
Fragments arranged by size:
437 381 181 162
XmaI C'CCGG_G
Cuts at: 0 499 720 1161
Size: 499 221 441
Fragments arranged by size:
499 441 221
ZraI GAC'GTC
Cuts at: 0 371 1161
Size: 371 790
Enzymes that do cut:
AarI AatII AcuI AflIII ApaLI AvaI BanI BanII
BbeI BciVI BglI BlpI Bme1580I BmgBI BpmI BpuEI
BsaI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI
BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrFI
BssHII BstAPI BstEII BstXI BstYI BtgI DrdI EaeI
EagI EarI EciI Eco57MI FalI FspI HaeII Hin4I
HincII KasI MluI MmeI NaeI NarI NgoMIV NspI
PasI PflMI PshAI SfcI SfoI SmaI SmlI SphI
TaqII XcmI XmaI ZraI
Enzymes that do not cut:
AccI Acc65I AclI AfeI AflII AgeI AhdI AleI
AlfI AloI AlwNI ApaI ApoI AscI AseI AsiSI
AvrII BaeI BamHI BbsI BbvCI BcgI BclI BfrBI
BglII BmrI BmtI BplI Bpu10I BsaAI BsaBI BsiWI
BsmBI BsrDI BsrGI BssSI BstBI BstZ17I Bsu36I BtgZI
BtsI ClaI CspCI DraI DraIII EcoICRI EcoNI EcoO109I
EcoRI EcoRV FseI FspAI HindIII HpaI KpnI MfeI
MscI MslI MspA1I NcoI NdeI NheI NotI NruI
NsiI PacI PciI PfoI PmeI PmlI PpiI PpuMI
PsiI PspOMI PspXI PsrI PstI PvuI PvuII RsrII
SacI SacII SalI SanDI SapI SbfI ScaI SexAI
SfiI SgfI SgrAI SnaBI SpeI SrfI SspI StuI
StyI SwaI TatI TstI Tth111I XbaI XhoI XmnI