(Circular) (Six-base) MAPSORT of: pFN21AB9669.seq Check: 4197 from: 1 to: 6172 pFN21AB9669 6172 bp With 182 enzymes: SgfI * October 20, 2009 10:51 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2150 3465 2150 Size: 1315 4857 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3982 278 Size: 53 83 186 936 2446 2468 Fragments arranged by size: 2468 2446 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3515 1937 Size: 1578 4594 Acc65I G'GTAC_C Cuts at: 3493 3493 Size: 6172 AclI AA'CG_TT Cuts at: 1563 4299 4672 1563 Size: 2736 373 3063 Fragments arranged by size: 3063 2736 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2258 2331 2572 2740 2824 2992 3076 3244 Size: 626 73 241 168 84 168 84 168 Cuts at: 3244 3412 4235 5619 5887 1632 Size: 168 823 1384 268 1917 Fragments arranged by size: 1917 1384 823 626 268 241 168 168 168 168 84 84 73 AfeI AGC'GCT Cuts at: 2513 3041 2513 Size: 528 5644 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5975 AflIII A'CryG_T Cuts at: 1203 2655 2730 2814 2982 3234 5071 6048 1203 Size: 1452 75 84 168 252 1837 977 1327 Fragments arranged by size: 1837 1452 1327 977 252 168 84 75 AgeI A'CCGG_T Cuts at: 5003 5003 Size: 6172 AhdI GACnn_n'nnGTC Cuts at: 4901 4901 Size: 6172 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 6172 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 6172 AlwNI CAG_nnn'CTG Cuts at: 3915 5487 3915 Size: 1572 4600 ApaLI G'TGCA_C Cuts at: 2540 2612 2780 3200 3212 3368 4229 5385 2540 Size: 72 168 420 12 156 861 1156 3327 Fragments arranged by size: 3327 1156 861 420 168 156 72 12 ApoI r'AATT_y Cuts at: 1493 2184 3481 3707 3859 5968 1493 Size: 691 1297 226 152 2109 1697 Fragments arranged by size: 2109 1697 1297 691 226 152 AseI AT'TA_AT Cuts at: 160 1032 4726 160 Size: 872 3694 1606 Fragments arranged by size: 3694 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6172 AvaI C'yCGr_G Cuts at: 1870 1943 2822 3497 1870 Size: 73 879 675 4545 Fragments arranged by size: 4545 879 675 73 BamHI G'GATC_C Cuts at: 2017 2454 2706 3294 3378 3502 3869 2017 Size: 437 252 588 84 124 367 4320 Fragments arranged by size: 4320 588 437 367 252 124 84 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2090 3493 4948 618 Size: 325 217 627 303 1403 1455 1842 Fragments arranged by size: 1842 1455 1403 627 325 303 217 BanII G_rGCy'C Cuts at: 1391 2216 2936 3020 3491 1391 Size: 825 720 84 471 4072 Fragments arranged by size: 4072 825 720 471 84 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2046 2684 3428 928 Size: 971 147 638 744 3672 Fragments arranged by size: 3672 971 744 638 147 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4363 4397 1903 Size: 34 2426 34 3678 Fragments arranged by size: 3678 2426 34 34 BciVI GTATCCnnnnn_n' Cuts at: 4064 5274 4064 Size: 1210 4962 BclI T'GATC_A Cuts at: 1548 1548 Size: 6172 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2143 4783 136 Size: 107 122 71 631 605 471 2640 1525 Fragments arranged by size: 2640 1525 631 605 471 122 107 71 BglII A'GATC_T Cuts at: 6167 6167 Size: 6172 BlpI GC'TnA_GC Cuts at: 3589 3589 Size: 6172 Bme1580I G_kGCm'C Cuts at: 1790 2544 2616 2784 2822 3204 3216 3372 4233 Size: 754 72 168 38 382 12 156 861 Cuts at: 4233 5389 1790 Size: 1156 2573 Fragments arranged by size: 2573 1156 861 754 382 168 156 72 38 12 BmgBI CAC'GTC Cuts at: 1318 2656 1318 Size: 1338 4834 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3903 4861 442 Size: 416 53 83 399 24 2486 958 1753 Fragments arranged by size: 2486 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6172 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2323 4832 1239 Size: 1084 2509 2579 Fragments arranged by size: 2579 2509 1084 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 4297 5162 5460 4297 Size: 865 298 5009 Fragments arranged by size: 5009 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4835 882 Size: 617 3336 2219 Fragments arranged by size: 3336 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 2815 2983 3235 3963 493 Size: 711 1611 168 252 728 2702 Fragments arranged by size: 2702 1611 728 711 252 168 BsaBI GATnn'nnATC Cuts at: 6166 6166 Size: 6172 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3979 4361 4996 275 Size: 53 83 186 936 2446 382 635 1451 Fragments arranged by size: 2446 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 2673 4605 5003 5277 5424 5835 2673 Size: 1932 398 274 147 411 3010 Fragments arranged by size: 3010 1932 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2033 2063 2795 2825 2879 Size: 30 165 30 630 30 732 30 54 Cuts at: 2879 2909 2918 2948 1178 Size: 30 9 30 4402 Fragments arranged by size: 4402 732 630 165 54 30 30 30 30 30 30 9 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2393 2926 3094 3346 1186 Size: 1207 533 168 252 4012 Fragments arranged by size: 4012 1207 533 252 168 BseYI C'CCAG_C Cuts at: 2694 5375 5871 2694 Size: 2681 496 2995 Fragments arranged by size: 2995 2681 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2184 2933 3091 1163 Size: 573 448 749 158 4244 Fragments arranged by size: 4244 749 573 448 158 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3622 4383 5411 1540 Size: 275 186 1621 761 1028 2301 Fragments arranged by size: 2301 1621 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2544 2616 2784 2868 2936 3020 3204 3216 3288 Size: 72 168 84 68 84 184 12 72 Cuts at: 3288 3372 4233 4318 5389 2544 Size: 84 861 85 1071 3327 Fragments arranged by size: 3327 1071 861 184 168 85 84 84 84 72 72 68 12 BsiWI C'GTAC_G Cuts at: 2811 2895 2811 Size: 84 6088 BsmI GAATG_Cn' Cuts at: 3683 3776 3683 Size: 93 6079 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2216 2544 2616 2784 2822 2868 2936 Size: 399 426 328 72 168 38 46 68 Cuts at: 2936 3020 3204 3216 3288 3372 3491 4233 4318 Size: 84 184 12 72 84 119 742 85 Cuts at: 4318 5389 1391 Size: 1071 2174 Fragments arranged by size: 2174 1071 742 426 399 328 184 168 119 85 84 84 72 72 68 46 38 12 BspEI T'CCGG_A Cuts at: 2673 5835 2673 Size: 3162 3010 BspHI T'CATG_A Cuts at: 2230 4061 5791 2230 Size: 1831 1730 2611 Fragments arranged by size: 2611 1831 1730 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2150 3465 3527 844 Size: 1306 1315 62 3489 Fragments arranged by size: 3489 1315 1306 62 BsrBI CCG'CTC Cuts at: 1394 1823 2704 4059 1394 Size: 429 881 1355 3507 Fragments arranged by size: 3507 1355 881 429 BsrDI GCAATG_nn' Cuts at: 65 1248 4667 4841 65 Size: 1183 3419 174 1396 Fragments arranged by size: 3419 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2966 3134 3302 4816 5003 1700 Size: 110 1156 168 168 1514 187 2869 Fragments arranged by size: 2869 1514 1156 187 168 168 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 6172 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6172 BssSI C'ACGA_G Cuts at: 2080 2699 4232 5244 2080 Size: 619 1533 1012 3008 Fragments arranged by size: 3008 1533 1012 619 BstAPI GCAn_nnn'nTGC Cuts at: 794 2910 3078 794 Size: 2116 168 3888 Fragments arranged by size: 3888 2116 168 BstBI TT'CG_AA Cuts at: 4976 4976 Size: 6172 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 6172 BstXI CCAn_nnnn'nTGG Cuts at: 2133 2133 Size: 6172 BstYI r'GATC_y Cuts at: 1972 2017 2454 2706 3294 3378 3502 3869 4254 Size: 45 437 252 588 84 124 367 385 Cuts at: 4254 4271 5723 5809 5821 6167 1972 Size: 17 1452 86 12 346 1977 Fragments arranged by size: 1977 1452 588 437 385 367 346 252 124 86 84 45 17 12 BtgI C'CryG_G Cuts at: 513 1065 2067 2147 2276 513 Size: 552 1002 80 129 4409 Fragments arranged by size: 4409 1002 552 129 80 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5433 Fragments arranged by size: 5433 683 56 BtsI GCAGTG_nn' Cuts at: 3688 4482 4502 3688 Size: 794 20 5358 Fragments arranged by size: 5358 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6172 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6137 DraI TTT'AAA Cuts at: 3476 3476 Size: 6172 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5179 809 Size: 507 3863 1802 Fragments arranged by size: 3863 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2135 3325 3619 Size: 54 1425 50 275 18 305 1190 294 Cuts at: 3619 4508 6137 8 Size: 889 1629 43 Fragments arranged by size: 1629 1425 1190 889 305 294 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3619 1537 Size: 275 1807 4090 Fragments arranged by size: 4090 1807 275 EarI CTCTTCn'nnn_ Cuts at: 1353 4102 1353 Size: 2749 3423 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4759 5133 5279 387 Size: 1064 138 3170 374 146 1280 Fragments arranged by size: 3170 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 2934 3018 2934 Size: 84 6088 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2258 2323 2331 2572 2740 2824 2992 Size: 393 626 65 8 241 168 84 168 Cuts at: 2992 3076 3244 3412 4235 4832 5619 5887 1239 Size: 84 168 168 823 597 787 268 1524 Fragments arranged by size: 1524 823 787 626 597 393 268 241 168 168 168 168 84 84 65 8 EcoO109I rG'GnC_Cy Cuts at: 2075 2099 2304 2075 Size: 24 205 5943 Fragments arranged by size: 5943 205 24 EcoRI G'AATT_C Cuts at: 2184 3481 3859 2184 Size: 1297 378 4497 Fragments arranged by size: 4497 1297 378 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 3190 3190 Size: 6172 FspI TGC'GCA Cuts at: 4678 4678 Size: 6172 HaeII r_GCGC'y Cuts at: 1678 2515 3043 3187 3273 5319 1678 Size: 837 528 144 86 2046 2531 Fragments arranged by size: 2531 2046 837 528 144 86 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2033 2065 2501 2533 2671 Size: 32 135 32 461 32 436 32 138 Cuts at: 2671 2703 4813 4845 4887 4919 1373 Size: 32 2110 32 42 32 2626 Fragments arranged by size: 2626 2110 461 436 138 135 42 32 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3516 3762 669 Size: 988 281 1578 246 3079 Fragments arranged by size: 3079 1578 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 6172 HpaI GTT'AAC Cuts at: 3762 3762 Size: 6172 KpnI G_GTAC'C Cuts at: 3497 3497 Size: 6172 MfeI C'AATT_G Cuts at: 3771 3771 Size: 6172 MluI A'CGCG_T Cuts at: 2730 6048 2730 Size: 3318 2854 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 1995 5286 5470 1305 Size: 690 3291 184 2007 Fragments arranged by size: 3291 2007 690 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2137 10 Size: 54 1425 343 305 4045 Fragments arranged by size: 4045 1425 343 305 54 MslI CAynn'nnrTG Cuts at: 518 1166 3053 4130 4489 4648 518 Size: 648 1887 1077 359 159 2042 Fragments arranged by size: 2042 1887 1077 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2133 2149 2536 3292 3376 3588 4265 5413 Size: 527 16 387 756 84 212 677 1148 Cuts at: 5413 5658 1606 Size: 245 2120 Fragments arranged by size: 2120 1148 756 677 527 387 245 212 84 16 NcoI C'CATG_G Cuts at: 513 1065 513 Size: 552 5620 NdeI CA'TA_TG Cuts at: 387 387 Size: 6172 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6172 NotI GC'GGCC_GC Cuts at: 3619 3619 Size: 6172 NspI r_CATG'y Cuts at: 1132 1229 2229 2770 2854 3530 5075 1132 Size: 97 1000 541 84 676 1545 2229 Fragments arranged by size: 2229 1545 1000 676 541 97 84 PasI CC'CwG_GG Cuts at: 1348 2191 1348 Size: 843 5329 PciI A'CATG_T Cuts at: 5071 5071 Size: 6172 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 6172 PmeI GTTT'AAAC Cuts at: 3476 3476 Size: 6172 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4241 4273 5754 5786 4241 Size: 32 1481 32 4627 Fragments arranged by size: 4627 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2075 2099 2075 Size: 24 6148 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6172 PsiI TTA'TAA Cuts at: 3742 3742 Size: 6172 PstI C_TGCA'G Cuts at: 830 2402 3524 830 Size: 1572 1122 3478 Fragments arranged by size: 3478 1572 1122 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6172 PvuII CAG'CTG Cuts at: 2133 2133 Size: 6172 SacI G_AGCT'C Cuts at: 2936 3020 2936 Size: 84 6088 SacII CC_GC'GG Cuts at: 2150 2150 Size: 6172 SalI G'TCGA_C Cuts at: 1936 3514 1936 Size: 1578 4594 SanDI GG'GwC_CC Cuts at: 2075 2075 Size: 6172 SbfI CC_TGCA'GG Cuts at: 3524 3524 Size: 6172 ScaI AGT'ACT Cuts at: 4420 6132 4420 Size: 1712 4460 SfcI C'TryA_G Cuts at: 826 1045 2398 3520 4655 5336 5527 826 Size: 219 1353 1122 1135 681 191 1471 Fragments arranged by size: 1471 1353 1135 1122 681 219 191 SfiI GGCCn_nnn'nGGCC Cuts at: 2143 2143 Size: 6172 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6172 SgrAI Cr'CCGG_yG Cuts at: 1700 2966 3134 3302 1700 Size: 1266 168 168 4570 Fragments arranged by size: 4570 1266 168 168 SmaI CCC'GGG Cuts at: 1872 3499 1872 Size: 1627 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 4276 5177 5439 820 Size: 197 926 2333 901 262 1553 Fragments arranged by size: 2333 1553 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 3963 493 Size: 3470 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6172 SphI G_CATG'C Cuts at: 1132 3530 1132 Size: 2398 3774 SspI AAT'ATT Cuts at: 5 52 4096 5 Size: 47 4044 2081 Fragments arranged by size: 4044 2081 47 StuI AGG'CCT Cuts at: 1517 2417 2585 2669 2753 2921 3005 3089 3257 Size: 900 168 84 84 168 84 84 168 Cuts at: 3257 1517 Size: 4432 Fragments arranged by size: 4432 900 168 168 168 84 84 84 84 StyI C'CwwG_G Cuts at: 513 1065 2254 3611 513 Size: 552 1189 1357 3074 Fragments arranged by size: 3074 1357 1189 552 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2708 2876 4199 4216 4369 4554 4999 Size: 1255 1128 168 1323 17 153 185 445 Cuts at: 4999 325 Size: 1498 Fragments arranged by size: 1498 1323 1255 1128 445 185 168 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 4418 6130 96 Size: 274 80 33 51 1443 2441 1712 138 Fragments arranged by size: 2441 1712 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 6132 6164 6132 Size: 32 6140 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 6172 XbaI T'CTAG_A Cuts at: 3508 3508 Size: 6172 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2011 2011 Size: 6172 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6172 XmaI C'CCGG_G Cuts at: 1870 3497 1870 Size: 1627 4545 XmnI GAAnn'nnTTC Cuts at: 2501 3062 3173 3341 3425 4301 2501 Size: 561 111 168 84 876 4372 Fragments arranged by size: 4372 876 561 168 111 84 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3980 276 Size: 53 83 186 936 2446 2468 Fragments arranged by size: 2468 2446 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AfeI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsiWI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PmeI PpiI PpuMI PshAI PsiI PstI PvuI PvuII SacI SacII SalI SanDI SbfI ScaI SfcI SfiI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AloI ApaI AscI AvrII BaeI BbeI BbvCI BfrBI BplI Bpu10I BsmBI BstZ17I Bsu36I DraIII EcoNI EcoRV FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PfoI PmlI PspOMI PspXI PsrI RsrII SapI SexAI SfoI SrfI SwaI