Restriction map for
pFN21AB9669
(Linear) (Six-base) MAPSORT of: KIBB9669.seq Check: 6682 from: 1 to: 1467
KIBB9669.orf
With 182 enzymes: SgfI *
April 24, 2009 13:08 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 145 1460 1467
Size: 145 1315 7
Fragments arranged by size:
1315 145 7
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 253 326 567 735 819 987 1071 1239
Size: 253 73 241 168 84 168 84 168
Cuts at: 1239 1407 1467
Size: 168 60
Fragments arranged by size:
253 241 168 168 168 168 84 84
73 60
AfeI AGC'GCT
Cuts at: 0 508 1036 1467
Size: 508 528 431
Fragments arranged by size:
528 508 431
AflIII A'CryG_T
Cuts at: 0 650 725 809 977 1229 1467
Size: 650 75 84 168 252 238
Fragments arranged by size:
650 252 238 168 84 75
ApaLI G'TGCA_C
Cuts at: 0 535 607 775 1195 1207 1363 1467
Size: 535 72 168 420 12 156 104
Fragments arranged by size:
535 420 168 156 104 72 12
ApoI r'AATT_y
Cuts at: 0 179 1467
Size: 179 1288
AvaI C'yCGr_G
Cuts at: 0 817 1467
Size: 817 650
BamHI G'GATC_C
Cuts at: 0 12 449 701 1289 1373 1467
Size: 12 437 252 588 84 94
Fragments arranged by size:
588 437 252 94 84 12
BanI G'GyrC_C
Cuts at: 0 85 1467
Size: 85 1382
BanII G_rGCy'C
Cuts at: 0 211 931 1015 1467
Size: 211 720 84 452
Fragments arranged by size:
720 452 211 84
BbsI GAAGACnn'nnnn_
Cuts at: 0 41 679 1423 1467
Size: 41 638 744 44
Fragments arranged by size:
744 638 44 41
BglI GCCn_nnn'nGGC
Cuts at: 0 138 1467
Size: 138 1329
Bme1580I G_kGCm'C
Cuts at: 0 539 611 779 817 1199 1211 1367 1467
Size: 539 72 168 38 382 12 156 100
Fragments arranged by size:
539 382 168 156 100 72 38 12
BmgBI CAC'GTC
Cuts at: 0 651 1467
Size: 651 816
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 318 1467
Size: 318 1149
BsaAI yAC'GTr
Cuts at: 0 810 978 1230 1467
Size: 810 168 252 237
Fragments arranged by size:
810 252 237 168
BsaWI w'CCGG_w
Cuts at: 0 668 1467
Size: 668 799
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 28 58 790 820 874 904 913 943
Size: 28 30 732 30 54 30 9 30
Cuts at: 943 1467
Size: 524
Fragments arranged by size:
732 524 54 30 30 30 30 28
9
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 388 921 1089 1341 1467
Size: 388 533 168 252 126
Fragments arranged by size:
533 388 252 168 126
BseYI C'CCAG_C
Cuts at: 0 689 1467
Size: 689 778
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 179 928 1086 1467
Size: 179 749 158 381
Fragments arranged by size:
749 381 179 158
BsiHKAI G_wGCw'C
Cuts at: 0 539 611 779 863 931 1015 1199 1211
Size: 539 72 168 84 68 84 184 12
Cuts at: 1211 1283 1367 1467
Size: 72 84 100
Fragments arranged by size:
539 184 168 100 84 84 84 72
72 68 12
BsiWI C'GTAC_G
Cuts at: 0 806 890 1467
Size: 806 84 577
Fragments arranged by size:
806 577 84
Bsp1286I G_dGCh'C
Cuts at: 0 211 539 611 779 817 863 931 1015
Size: 211 328 72 168 38 46 68 84
Cuts at: 1015 1199 1211 1283 1367 1467
Size: 184 12 72 84 100
Fragments arranged by size:
328 211 184 168 100 84 84 72
72 68 46 38 12
BspEI T'CCGG_A
Cuts at: 0 668 1467
Size: 668 799
BspHI T'CATG_A
Cuts at: 0 225 1467
Size: 225 1242
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 145 1460 1467
Size: 145 1315 7
Fragments arranged by size:
1315 145 7
BsrBI CCG'CTC
Cuts at: 0 699 1467
Size: 699 768
BsrFI r'CCGG_y
Cuts at: 0 961 1129 1297 1467
Size: 961 168 168 170
Fragments arranged by size:
961 170 168 168
BssSI C'ACGA_G
Cuts at: 0 75 694 1467
Size: 75 619 773
Fragments arranged by size:
773 619 75
BstAPI GCAn_nnn'nTGC
Cuts at: 0 905 1073 1467
Size: 905 168 394
Fragments arranged by size:
905 394 168
BstXI CCAn_nnnn'nTGG
Cuts at: 0 128 1467
Size: 128 1339
BstYI r'GATC_y
Cuts at: 0 12 449 701 1289 1373 1467
Size: 12 437 252 588 84 94
Fragments arranged by size:
588 437 252 94 84 12
BtgI C'CryG_G
Cuts at: 0 62 142 271 1467
Size: 62 80 129 1196
Fragments arranged by size:
1196 129 80 62
EaeI y'GGCC_r
Cuts at: 0 130 1320 1467
Size: 130 1190 147
Fragments arranged by size:
1190 147 130
EcoICRI GAG'CTC
Cuts at: 0 929 1013 1467
Size: 929 84 454
Fragments arranged by size:
929 454 84
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 253 318 326 567 735 819 987 1071
Size: 253 65 8 241 168 84 168 84
Cuts at: 1071 1239 1407 1467
Size: 168 168 60
Fragments arranged by size:
253 241 168 168 168 168 84 84
65 60 8
EcoO109I rG'GnC_Cy
Cuts at: 0 70 94 299 1467
Size: 70 24 205 1168
Fragments arranged by size:
1168 205 70 24
EcoRI G'AATT_C
Cuts at: 0 179 1467
Size: 179 1288
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 1185 1467
Size: 1185 282
HaeII r_GCGC'y
Cuts at: 0 510 1038 1182 1268 1467
Size: 510 528 144 86 199
Fragments arranged by size:
528 510 199 144 86
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 28 60 496 528 666 698 1467
Size: 28 32 436 32 138 32 769
Fragments arranged by size:
769 436 138 32 32 32 28
MluI A'CGCG_T
Cuts at: 0 725 1467
Size: 725 742
MscI TGG'CCA
Cuts at: 0 132 1467
Size: 132 1335
MslI CAynn'nnrTG
Cuts at: 0 1048 1467
Size: 1048 419
MspA1I CmG'CkG
Cuts at: 0 128 144 531 1287 1371 1467
Size: 128 16 387 756 84 96
Fragments arranged by size:
756 387 128 96 84 16
NspI r_CATG'y
Cuts at: 0 224 765 849 1467
Size: 224 541 84 618
Fragments arranged by size:
618 541 224 84
PasI CC'CwG_GG
Cuts at: 0 186 1467
Size: 186 1281
PpuMI rG'GwC_Cy
Cuts at: 0 70 94 1467
Size: 70 24 1373
Fragments arranged by size:
1373 70 24
PstI C_TGCA'G
Cuts at: 0 397 1467
Size: 397 1070
PvuII CAG'CTG
Cuts at: 0 128 1467
Size: 128 1339
SacI G_AGCT'C
Cuts at: 0 931 1015 1467
Size: 931 84 452
Fragments arranged by size:
931 452 84
SacII CC_GC'GG
Cuts at: 0 145 1467
Size: 145 1322
SanDI GG'GwC_CC
Cuts at: 0 70 1467
Size: 70 1397
SfcI C'TryA_G
Cuts at: 0 393 1467
Size: 393 1074
SfiI GGCCn_nnn'nGGCC
Cuts at: 0 138 1467
Size: 138 1329
SgrAI Cr'CCGG_yG
Cuts at: 0 961 1129 1297 1467
Size: 961 168 168 170
Fragments arranged by size:
961 170 168 168
StuI AGG'CCT
Cuts at: 0 412 580 664 748 916 1000 1084 1252
Size: 412 168 84 84 168 84 84 168
Cuts at: 1252 1467
Size: 215
Fragments arranged by size:
412 215 168 168 168 84 84 84
84
StyI C'CwwG_G
Cuts at: 0 249 1467
Size: 249 1218
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 703 871 1467
Size: 703 168 596
Fragments arranged by size:
703 596 168
XmnI GAAnn'nnTTC
Cuts at: 0 496 1057 1168 1336 1420 1467
Size: 496 561 111 168 84 47
Fragments arranged by size:
561 496 168 111 84 47
Enzymes that do cut:
AarI AcuI AfeI AflIII ApaLI ApoI AvaI BamHI
BanI BanII BbsI BglI Bme1580I BmgBI BpmI BsaAI
BsaWI BsaXI BseRI BseYI BsgI BsiHKAI BsiWI Bsp1286I
BspEI BspHI BspMI BsrBI BsrFI BssSI BstAPI BstXI
BstYI BtgI EaeI EcoICRI Eco57MI EcoO109I EcoRI FalI
HaeII Hin4I MluI MscI MslI MspA1I NspI PasI
PpuMI PstI PvuII SacI SacII SanDI SfcI SfiI
SgrAI StuI StyI TaqII XmnI
Enzymes that do not cut:
AatII AccI Acc65I AclI AflII AgeI AhdI AleI
AlfI AloI AlwNI ApaI AscI AseI AsiSI AvrII
BaeI BbeI BbvCI BcgI BciVI BclI BfrBI BglII
BlpI BmrI BmtI BplI Bpu10I BpuEI BsaI BsaBI
BsaHI BsiEI BsmI BsmBI BsrDI BsrGI BssHII BstBI
BstEII BstZ17I Bsu36I BtgZI BtsI ClaI CspCI DraI
DraIII DrdI EagI EarI EciI EcoNI EcoRV FseI
FspI FspAI HincII HindIII HpaI KasI KpnI MfeI
MmeI NaeI NarI NcoI NdeI NgoMIV NheI NotI
NruI NsiI PacI PciI PflMI PfoI PmeI PmlI
PpiI PshAI PsiI PspOMI PspXI PsrI PvuI RsrII
SalI SapI SbfI ScaI SexAI SfoI SgfI SmaI
SmlI SnaBI SpeI SphI SrfI SspI SwaI TatI
TstI Tth111I XbaI XcmI XhoI XmaI ZraI