(Circular) (Six-base) MAPSORT of: pFN21AB9594.seq Check: 4696 from: 1 to: 5680 pFN21AB9594 5680 bp With 182 enzymes: SgfI * October 20, 2009 10:51 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2451 2451 Size: 5680 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3490 278 Size: 53 83 186 936 1954 2468 Fragments arranged by size: 2468 1954 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3023 1937 Size: 1086 4594 Acc65I G'GTAC_C Cuts at: 3001 3001 Size: 5680 AclI AA'CG_TT Cuts at: 1563 3807 4180 1563 Size: 2244 373 3063 Fragments arranged by size: 3063 2244 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 3743 5127 5395 1632 Size: 2111 1384 268 1917 Fragments arranged by size: 2111 1917 1384 268 AfeI AGC'GCT Cuts at: 2171 2327 2171 Size: 156 5524 AflII C'TTAA_G Cuts at: 820 1017 2339 820 Size: 197 1322 4161 Fragments arranged by size: 4161 1322 197 AflIII A'CryG_T Cuts at: 1203 2025 2289 2653 2655 4579 5556 1203 Size: 822 264 364 2 1924 977 1327 Fragments arranged by size: 1924 1327 977 822 364 264 2 AgeI A'CCGG_T Cuts at: 4511 4511 Size: 5680 AhdI GACnn_n'nnGTC Cuts at: 4409 4409 Size: 5680 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 5680 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 2327 2459 1184 Size: 1143 132 4405 Fragments arranged by size: 4405 1143 132 AlwNI CAG_nnn'CTG Cuts at: 2262 2389 3423 4995 2262 Size: 127 1034 1572 2947 Fragments arranged by size: 2947 1572 1034 127 ApaLI G'TGCA_C Cuts at: 2975 3737 4893 2975 Size: 762 1156 3762 Fragments arranged by size: 3762 1156 762 ApoI r'AATT_y Cuts at: 1493 2989 3215 3367 5476 1493 Size: 1496 226 152 2109 1697 Fragments arranged by size: 2109 1697 1496 226 152 AseI AT'TA_AT Cuts at: 160 1032 4234 160 Size: 872 3202 1606 Fragments arranged by size: 3202 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5680 AvaI C'yCGr_G Cuts at: 1870 1943 2093 2431 2494 3005 1870 Size: 73 150 338 63 511 4545 Fragments arranged by size: 4545 511 338 150 73 63 BamHI G'GATC_C Cuts at: 3010 3377 3010 Size: 367 5313 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2316 2356 2584 2639 3001 Size: 325 217 627 529 40 228 55 362 Cuts at: 3001 4456 618 Size: 1455 1842 Fragments arranged by size: 1842 1455 627 529 362 325 228 217 55 40 BanII G_rGCy'C Cuts at: 1391 2090 2999 1391 Size: 699 909 4072 Fragments arranged by size: 4072 909 699 BbeI G_GCGC'C Cuts at: 2320 2360 2320 Size: 40 5640 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 4709 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 1987 2021 3871 3905 1903 Size: 34 50 34 1850 34 3678 Fragments arranged by size: 3678 1850 50 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3572 4782 3572 Size: 1210 4470 BclI T'GATC_A Cuts at: 1548 1548 Size: 5680 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2378 2557 4291 Size: 107 122 71 631 605 706 179 1734 Cuts at: 4291 136 Size: 1525 Fragments arranged by size: 1734 1525 706 631 605 179 122 107 71 BglII A'GATC_T Cuts at: 2118 5675 2118 Size: 3557 2123 BlpI GC'TnA_GC Cuts at: 2111 2414 2876 3097 2111 Size: 303 462 221 4694 Fragments arranged by size: 4694 462 303 221 Bme1580I G_kGCm'C Cuts at: 1790 2438 2589 2642 2790 2817 2979 3741 4897 Size: 648 151 53 148 27 162 762 1156 Cuts at: 4897 1790 Size: 2573 Fragments arranged by size: 2573 1156 762 648 162 151 148 53 27 BmgBI CAC'GTC Cuts at: 1318 2886 1318 Size: 1568 4112 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3411 4369 442 Size: 416 53 83 399 24 1994 958 1753 Fragments arranged by size: 1994 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5680 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 4340 1239 Size: 3101 2579 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3805 4670 4968 3805 Size: 865 298 4517 Fragments arranged by size: 4517 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4343 882 Size: 617 2844 2219 Fragments arranged by size: 2844 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 2028 2656 3471 493 Size: 711 824 628 815 2702 Fragments arranged by size: 2702 824 815 711 628 BsaBI GATnn'nnATC Cuts at: 5674 5674 Size: 5680 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2317 2357 3487 3869 Size: 53 83 186 936 784 40 1130 382 Cuts at: 3869 4504 275 Size: 635 1451 Fragments arranged by size: 1451 1130 936 784 635 382 186 83 53 40 BsaWI w'CCGG_w Cuts at: 4113 4511 4785 4932 5343 4113 Size: 398 274 147 411 4450 Fragments arranged by size: 4450 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2183 2213 1178 Size: 30 165 30 780 30 4645 Fragments arranged by size: 4645 780 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 1186 Size: 5680 BseYI C'CCAG_C Cuts at: 2360 2635 2927 4883 5379 2360 Size: 275 292 1956 496 2661 Fragments arranged by size: 2661 1956 496 292 275 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2255 1163 Size: 573 519 4588 Fragments arranged by size: 4588 573 519 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2020 2237 2808 3130 3891 4919 Size: 275 186 19 217 571 322 761 1028 Cuts at: 4919 1540 Size: 2301 Fragments arranged by size: 2301 1028 761 571 322 275 217 186 19 BsiHKAI G_wGCw'C Cuts at: 2090 2979 3741 3826 4897 2090 Size: 889 762 85 1071 2873 Fragments arranged by size: 2873 1071 889 762 85 BsmI GAATG_Cn' Cuts at: 3191 3284 3191 Size: 93 5587 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2090 2438 2589 2642 2790 2817 2979 Size: 399 300 348 151 53 148 27 162 Cuts at: 2979 2999 3741 3826 4897 1391 Size: 20 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 399 348 300 162 151 148 85 53 27 20 BspEI T'CCGG_A Cuts at: 5343 5343 Size: 5680 BspHI T'CATG_A Cuts at: 3569 5299 3569 Size: 1730 3950 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2397 2451 3035 844 Size: 1553 54 584 3489 Fragments arranged by size: 3489 1553 584 54 BsrBI CCG'CTC Cuts at: 1394 1823 2602 3567 1394 Size: 429 779 965 3507 Fragments arranged by size: 3507 965 779 429 BsrDI GCAATG_nn' Cuts at: 65 1248 4175 4349 65 Size: 1183 2927 174 1396 Fragments arranged by size: 2927 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2581 2642 2669 2803 4324 4511 1700 Size: 110 771 61 27 134 1521 187 2869 Fragments arranged by size: 2869 1521 771 187 134 110 61 27 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5680 BssHII G'CGCG_C Cuts at: 1925 2418 1925 Size: 493 5187 BssSI C'ACGA_G Cuts at: 3740 4752 3740 Size: 1012 4668 BstAPI GCAn_nnn'nTGC Cuts at: 794 2389 2584 794 Size: 1595 195 3890 Fragments arranged by size: 3890 1595 195 BstBI TT'CG_AA Cuts at: 4484 4484 Size: 5680 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5680 BstXI CCAn_nnnn'nTGG Cuts at: 2701 2701 Size: 5680 BstYI r'GATC_y Cuts at: 1972 2118 2223 3010 3377 3762 3779 5231 5317 Size: 146 105 787 367 385 17 1452 86 Cuts at: 5317 5329 5675 1972 Size: 12 346 1977 Fragments arranged by size: 1977 1452 787 385 367 346 146 105 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2228 2622 2700 513 Size: 552 1163 394 78 3493 Fragments arranged by size: 3493 1163 552 394 78 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 2515 487 Size: 683 56 1289 3652 Fragments arranged by size: 3652 1289 683 56 BtsI GCAGTG_nn' Cuts at: 3196 3990 4010 3196 Size: 794 20 4866 Fragments arranged by size: 4866 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5680 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5645 DraI TTT'AAA Cuts at: 2984 2984 Size: 5680 DraIII CAC_nnn'GTG Cuts at: 2968 2968 Size: 5680 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4687 809 Size: 507 3371 1802 Fragments arranged by size: 3371 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2017 2076 2234 Size: 54 1425 50 275 18 187 59 158 Cuts at: 2234 2648 2685 2805 3127 4016 5645 8 Size: 414 37 120 322 889 1629 43 Fragments arranged by size: 1629 1425 889 414 322 275 187 158 120 59 54 50 43 37 18 EagI C'GGCC_G Cuts at: 1537 1812 2017 2234 2805 3127 1537 Size: 275 205 217 571 322 4090 Fragments arranged by size: 4090 571 322 275 217 205 EarI CTCTTCn'nnn_ Cuts at: 1353 3610 1353 Size: 2257 3423 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2560 2656 2944 4267 4641 4787 Size: 1064 138 971 96 288 1323 374 146 Cuts at: 4787 387 Size: 1280 Fragments arranged by size: 1323 1280 1064 971 374 288 146 138 96 EcoICRI GAG'CTC Cuts at: 2088 2088 Size: 5680 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 3743 4340 5127 5395 1239 Size: 393 2111 597 787 268 1524 Fragments arranged by size: 2111 1524 787 597 393 268 EcoNI CCTnn'n_nnAGG Cuts at: 2152 2152 Size: 5680 EcoRI G'AATT_C Cuts at: 2989 3367 2989 Size: 378 5302 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2275 2527 2275 Size: 252 5428 FspI TGC'GCA Cuts at: 4186 4186 Size: 5680 HaeII r_GCGC'y Cuts at: 1678 2173 2270 2320 2329 2360 2724 2840 4827 Size: 495 97 50 9 31 364 116 1987 Cuts at: 4827 1678 Size: 2531 Fragments arranged by size: 2531 1987 495 364 116 97 50 31 9 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 4321 4353 4395 4427 1373 Size: 32 135 32 2749 32 42 32 2626 Fragments arranged by size: 2749 2626 135 42 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2616 3024 3270 669 Size: 988 281 678 408 246 3079 Fragments arranged by size: 3079 988 678 408 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 5680 HpaI GTT'AAC Cuts at: 3270 3270 Size: 5680 KasI G'GCGC_C Cuts at: 2316 2356 2316 Size: 40 5640 KpnI G_GTAC'C Cuts at: 3005 3005 Size: 5680 MfeI C'AATT_G Cuts at: 3279 3279 Size: 5680 MluI A'CGCG_T Cuts at: 5556 5556 Size: 5680 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2370 4794 4978 1305 Size: 1065 2424 184 2007 Fragments arranged by size: 2424 2007 1065 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2078 2650 10 Size: 54 1425 343 246 572 3040 Fragments arranged by size: 3040 1425 572 343 246 54 MslI CAynn'nnrTG Cuts at: 518 1166 2658 2699 2783 3638 3997 4156 518 Size: 648 1492 41 84 855 359 159 2042 Fragments arranged by size: 2042 1492 855 648 359 159 84 41 MspA1I CmG'CkG Cuts at: 1606 2110 2230 2591 2746 3096 3773 4921 5166 Size: 504 120 361 155 350 677 1148 245 Cuts at: 5166 1606 Size: 2120 Fragments arranged by size: 2120 1148 677 504 361 350 245 155 120 NaeI GCC'GGC Cuts at: 2671 2805 2671 Size: 134 5546 NarI GG'CG_CC Cuts at: 2317 2357 2317 Size: 40 5640 NcoI C'CATG_G Cuts at: 513 1065 2622 2700 513 Size: 552 1557 78 3493 Fragments arranged by size: 3493 1557 552 78 NdeI CA'TA_TG Cuts at: 387 387 Size: 5680 NgoMIV G'CCGG_C Cuts at: 2669 2803 2669 Size: 134 5546 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5680 NotI GC'GGCC_GC Cuts at: 3127 3127 Size: 5680 NspI r_CATG'y Cuts at: 1132 1229 2293 3038 4583 1132 Size: 97 1064 745 1545 2229 Fragments arranged by size: 2229 1545 1064 745 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 5680 PciI A'CATG_T Cuts at: 2289 4579 2289 Size: 2290 3390 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 5680 PmeI GTTT'AAAC Cuts at: 2984 2984 Size: 5680 PmlI CAC'GTG Cuts at: 2656 2656 Size: 5680 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3749 3781 5262 5294 3749 Size: 32 1481 32 4135 Fragments arranged by size: 4135 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5680 PsiI TTA'TAA Cuts at: 3250 3250 Size: 5680 PstI C_TGCA'G Cuts at: 830 2394 3032 830 Size: 1564 638 3478 Fragments arranged by size: 3478 1564 638 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5680 SacI G_AGCT'C Cuts at: 2090 2090 Size: 5680 SacII CC_GC'GG Cuts at: 2231 2231 Size: 5680 SalI G'TCGA_C Cuts at: 1936 3022 1936 Size: 1086 4594 SbfI CC_TGCA'GG Cuts at: 3032 3032 Size: 5680 ScaI AGT'ACT Cuts at: 3928 5640 3928 Size: 1712 3968 SfcI C'TryA_G Cuts at: 826 1045 2152 2390 2716 3028 4163 4844 5035 Size: 219 1107 238 326 312 1135 681 191 Cuts at: 5035 826 Size: 1471 Fragments arranged by size: 1471 1135 1107 681 326 312 238 219 191 SfoI GGC'GCC Cuts at: 2318 2358 2318 Size: 40 5640 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5680 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5680 SmaI CCC'GGG Cuts at: 1872 3007 1872 Size: 1135 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2339 3784 4685 4947 820 Size: 197 926 396 1445 901 262 1553 Fragments arranged by size: 1553 1445 926 901 396 262 197 SnaBI TAC'GTA Cuts at: 493 3471 493 Size: 2978 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5680 SphI G_CATG'C Cuts at: 1132 3038 1132 Size: 1906 3774 SspI AAT'ATT Cuts at: 5 52 3604 5 Size: 47 3552 2081 Fragments arranged by size: 3552 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 5680 StyI C'CwwG_G Cuts at: 513 1065 2622 2700 3119 513 Size: 552 1557 78 419 3074 Fragments arranged by size: 3074 1557 552 419 78 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2289 3707 3724 3877 4062 4507 325 Size: 1255 709 1418 17 153 185 445 1498 Fragments arranged by size: 1498 1418 1255 709 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 3926 5638 96 Size: 274 80 33 51 1443 1949 1712 138 Fragments arranged by size: 1949 1712 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2183 2215 5640 5672 2183 Size: 32 3425 32 2191 Fragments arranged by size: 3425 2191 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5680 XbaI T'CTAG_A Cuts at: 3016 3016 Size: 5680 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5680 XmaI C'CCGG_G Cuts at: 1870 3005 1870 Size: 1135 4545 XmnI GAAnn'nnTTC Cuts at: 3809 3809 Size: 5680 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3488 276 Size: 53 83 186 936 1954 2468 Fragments arranged by size: 2468 1954 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AfeI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PmeI PmlI PpiI PshAI PsiI PstI PvuI SacI SacII SalI SbfI ScaI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AloI ApaI AscI AvrII BaeI BbvCI BfrBI BplI Bpu10I BsiWI BsmBI BstZ17I Bsu36I EcoO109I EcoRV FseI FspAI NruI NsiI PacI PfoI PpuMI PspOMI PspXI PsrI PvuII RsrII SanDI SapI SexAI SfiI SrfI SwaI XcmI