(Circular) (Six-base) MAPSORT of: pFN21AB7945.seq Check: 3766 from: 1 to: 5878 pFN21AB7945 5878 bp With 182 enzymes: SgfI * April 15, 2010 09:24 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2491 3157 2491 Size: 666 5212 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3688 278 Size: 53 83 186 936 2152 2468 Fragments arranged by size: 2468 2152 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3221 1937 Size: 1284 4594 Acc65I G'GTAC_C Cuts at: 2946 3199 2946 Size: 253 5625 AclI AA'CG_TT Cuts at: 1563 4005 4378 1563 Size: 2442 373 3063 Fragments arranged by size: 3063 2442 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 3941 5325 5593 1632 Size: 2309 1384 268 1917 Fragments arranged by size: 2309 1917 1384 268 AfeI AGC'GCT Cuts at: 3124 3124 Size: 5878 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5681 AflIII A'CryG_T Cuts at: 1203 2956 4777 5754 1203 Size: 1753 1821 977 1327 Fragments arranged by size: 1821 1753 1327 977 AgeI A'CCGG_T Cuts at: 4709 4709 Size: 5878 AhdI GACnn_n'nnGTC Cuts at: 4607 4607 Size: 5878 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 5878 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 5878 AlwNI CAG_nnn'CTG Cuts at: 2157 2704 3621 5193 2157 Size: 547 917 1572 2842 Fragments arranged by size: 2842 1572 917 547 ApaLI G'TGCA_C Cuts at: 3935 5091 3935 Size: 1156 4722 ApoI r'AATT_y Cuts at: 1493 2205 3187 3413 3565 5674 1493 Size: 712 982 226 152 2109 1697 Fragments arranged by size: 2109 1697 982 712 226 152 AseI AT'TA_AT Cuts at: 160 1032 4432 160 Size: 872 3400 1606 Fragments arranged by size: 3400 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5878 AvaI C'yCGr_G Cuts at: 1870 1943 2777 2942 3091 3132 3203 1870 Size: 73 834 165 149 41 71 4545 Fragments arranged by size: 4545 834 165 149 73 71 41 BamHI G'GATC_C Cuts at: 2258 3208 3575 2258 Size: 950 367 4561 Fragments arranged by size: 4561 950 367 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2626 2946 3010 3199 4654 Size: 325 217 627 839 320 64 189 1455 Cuts at: 4654 618 Size: 1842 Fragments arranged by size: 1842 1455 839 627 325 320 217 189 64 BanII G_rGCy'C Cuts at: 1391 2769 3197 1391 Size: 1378 428 4072 Fragments arranged by size: 4072 1378 428 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 4907 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4069 4103 1903 Size: 34 2132 34 3678 Fragments arranged by size: 3678 2132 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3770 4980 3770 Size: 1210 4668 BclI T'GATC_A Cuts at: 1548 1548 Size: 5878 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2813 2846 4489 Size: 107 122 71 631 605 1141 33 1643 Cuts at: 4489 136 Size: 1525 Fragments arranged by size: 1643 1525 1141 631 605 122 107 71 33 BglII A'GATC_T Cuts at: 5873 5873 Size: 5878 BlpI GC'TnA_GC Cuts at: 3295 3295 Size: 5878 Bme1580I G_kGCm'C Cuts at: 1790 3939 5095 1790 Size: 2149 1156 2573 Fragments arranged by size: 2573 2149 1156 BmgBI CAC'GTC Cuts at: 1318 2568 2959 1318 Size: 1250 391 4237 Fragments arranged by size: 4237 1250 391 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3609 4567 442 Size: 416 53 83 399 24 2192 958 1753 Fragments arranged by size: 2192 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5878 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2678 2678 Size: 5878 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2152 2726 3097 4538 1239 Size: 913 574 371 1441 2579 Fragments arranged by size: 2579 1441 913 574 371 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 4003 4868 5166 4003 Size: 865 298 4715 Fragments arranged by size: 4715 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4541 882 Size: 617 3042 2219 Fragments arranged by size: 3042 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 3669 493 Size: 711 2465 2702 Fragments arranged by size: 2702 2465 711 BsaBI GATnn'nnATC Cuts at: 5872 5872 Size: 5878 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3685 4067 4702 275 Size: 53 83 186 936 2152 382 635 1451 Fragments arranged by size: 2152 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 2421 2623 2664 4311 4709 4983 5130 5541 2421 Size: 202 41 1647 398 274 147 411 2758 Fragments arranged by size: 2758 1647 411 398 274 202 147 41 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2085 2115 3056 3086 1178 Size: 30 165 30 682 30 941 30 3970 Fragments arranged by size: 3970 941 682 165 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2022 2073 3150 1186 Size: 836 51 1077 3914 Fragments arranged by size: 3914 1077 836 51 BseYI C'CCAG_C Cuts at: 2458 2573 5081 5577 2458 Size: 115 2508 496 2759 Fragments arranged by size: 2759 2508 496 115 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 1163 Size: 573 5305 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2529 3328 4089 5117 1540 Size: 275 186 528 799 761 1028 2301 Fragments arranged by size: 2301 1028 799 761 528 275 186 BsiHKAI G_wGCw'C Cuts at: 2519 2933 3939 4024 5095 2519 Size: 414 1006 85 1071 3302 Fragments arranged by size: 3302 1071 1006 414 85 BsmI GAATG_Cn' Cuts at: 3389 3482 3389 Size: 93 5785 BsmBI CGTCTCn'nnnn_ Cuts at: 2595 2595 Size: 5878 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2519 2769 2933 3197 3939 4024 5095 Size: 399 729 250 164 264 742 85 1071 Cuts at: 5095 1391 Size: 2174 Fragments arranged by size: 2174 1071 742 729 399 264 250 164 85 BspEI T'CCGG_A Cuts at: 2421 2664 5541 2421 Size: 243 2877 2758 Fragments arranged by size: 2877 2758 243 BspHI T'CATG_A Cuts at: 3767 5497 3767 Size: 1730 4148 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2205 2491 3157 3233 844 Size: 1361 286 666 76 3489 Fragments arranged by size: 3489 1361 666 286 76 BsrBI CCG'CTC Cuts at: 1394 1823 2368 3765 1394 Size: 429 545 1397 3507 Fragments arranged by size: 3507 1397 545 429 BsrDI GCAATG_nn' Cuts at: 65 1248 4373 4547 65 Size: 1183 3125 174 1396 Fragments arranged by size: 3125 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2352 2528 4522 4709 1700 Size: 110 542 176 1994 187 2869 Fragments arranged by size: 2869 1994 542 187 176 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5878 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5878 BssSI C'ACGA_G Cuts at: 3938 4950 3938 Size: 1012 4866 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 5878 BstBI TT'CG_AA Cuts at: 4682 4682 Size: 5878 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5878 BstXI CCAn_nnnn'nTGG Cuts at: 2290 2290 Size: 5878 BstYI r'GATC_y Cuts at: 1972 2258 3208 3575 3960 3977 5429 5515 5527 Size: 286 950 367 385 17 1452 86 12 Cuts at: 5527 5873 1972 Size: 346 1977 Fragments arranged by size: 1977 1452 950 385 367 346 286 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2004 2188 2802 513 Size: 552 939 184 614 3589 Fragments arranged by size: 3589 939 614 552 184 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5139 Fragments arranged by size: 5139 683 56 BtsI GCAGTG_nn' Cuts at: 2884 3159 3394 4188 4208 2884 Size: 275 235 794 20 4554 Fragments arranged by size: 4554 794 275 235 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5878 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 2314 2349 529 Size: 35 1750 35 4058 Fragments arranged by size: 4058 1750 35 35 DraI TTT'AAA Cuts at: 3182 3182 Size: 5878 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 2503 4885 809 Size: 507 1187 2382 1802 Fragments arranged by size: 2382 1802 1187 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2096 2526 2635 Size: 54 1425 50 275 18 266 430 109 Cuts at: 2635 2847 3325 4214 5843 8 Size: 212 478 889 1629 43 Fragments arranged by size: 1629 1425 889 478 430 275 266 212 109 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 2526 3325 1537 Size: 275 714 799 4090 Fragments arranged by size: 4090 799 714 275 EarI CTCTTCn'nnn_ Cuts at: 1353 3808 1353 Size: 2455 3423 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2105 4465 4839 4985 387 Size: 1064 138 516 2360 374 146 1280 Fragments arranged by size: 2360 1280 1064 516 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2152 2726 3097 3941 4538 5325 5593 Size: 393 520 574 371 844 597 787 268 Cuts at: 5593 1239 Size: 1524 Fragments arranged by size: 1524 844 787 597 574 520 393 371 268 EcoO109I rG'GnC_Cy Cuts at: 2154 2154 Size: 5878 EcoRI G'AATT_C Cuts at: 3187 3565 3187 Size: 378 5500 FspI TGC'GCA Cuts at: 4384 4384 Size: 5878 HaeII r_GCGC'y Cuts at: 1678 2580 3126 5025 1678 Size: 902 546 1899 2531 Fragments arranged by size: 2531 1899 902 546 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2085 2117 4519 4551 4593 Size: 32 135 32 513 32 2402 32 42 Cuts at: 4593 4625 1373 Size: 32 2626 Fragments arranged by size: 2626 2402 513 135 42 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3222 3468 669 Size: 988 281 1284 246 3079 Fragments arranged by size: 3079 1284 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 5878 HpaI GTT'AAC Cuts at: 3468 3468 Size: 5878 KpnI G_GTAC'C Cuts at: 2950 3203 2950 Size: 253 5625 MfeI C'AATT_G Cuts at: 3477 3477 Size: 5878 MluI A'CGCG_T Cuts at: 5754 5754 Size: 5878 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2051 4992 5176 1305 Size: 746 2941 184 2007 Fragments arranged by size: 2941 2007 746 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2849 10 Size: 54 1425 343 1017 3039 Fragments arranged by size: 3039 1425 1017 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2145 2193 3836 4195 4354 518 Size: 648 979 48 1643 359 159 2042 Fragments arranged by size: 2042 1643 979 648 359 159 48 MspA1I CmG'CkG Cuts at: 1606 2550 2704 2969 3294 3971 5119 5364 1606 Size: 944 154 265 325 677 1148 245 2120 Fragments arranged by size: 2120 1148 944 677 325 265 245 154 NaeI GCC'GGC Cuts at: 2530 2530 Size: 5878 NcoI C'CATG_G Cuts at: 513 1065 2004 2188 2802 513 Size: 552 939 184 614 3589 Fragments arranged by size: 3589 939 614 552 184 NdeI CA'TA_TG Cuts at: 387 387 Size: 5878 NgoMIV G'CCGG_C Cuts at: 2528 2528 Size: 5878 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5878 NotI GC'GGCC_GC Cuts at: 3325 3325 Size: 5878 NspI r_CATG'y Cuts at: 1132 1229 3236 4781 1132 Size: 97 2007 1545 2229 Fragments arranged by size: 2229 2007 1545 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 5878 PciI A'CATG_T Cuts at: 4777 4777 Size: 5878 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 5878 PfoI T'CCnGG_A Cuts at: 2103 3029 3074 2103 Size: 926 45 4907 Fragments arranged by size: 4907 926 45 PmeI GTTT'AAAC Cuts at: 3182 3182 Size: 5878 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2624 2656 3947 3979 5460 5492 2624 Size: 32 1291 32 1481 32 3010 Fragments arranged by size: 3010 1481 1291 32 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5878 PsiI TTA'TAA Cuts at: 3448 3448 Size: 5878 PstI C_TGCA'G Cuts at: 830 3154 3230 830 Size: 2324 76 3478 Fragments arranged by size: 3478 2324 76 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5878 PvuII CAG'CTG Cuts at: 2704 2704 Size: 5878 SalI G'TCGA_C Cuts at: 1936 3220 1936 Size: 1284 4594 SbfI CC_TGCA'GG Cuts at: 3230 3230 Size: 5878 ScaI AGT'ACT Cuts at: 4126 5838 4126 Size: 1712 4166 SfcI C'TryA_G Cuts at: 826 1045 3040 3150 3226 4361 5042 5233 826 Size: 219 1995 110 76 1135 681 191 1471 Fragments arranged by size: 1995 1471 1135 681 219 191 110 76 SfiI GGCCn_nnn'nGGCC Cuts at: 2846 2846 Size: 5878 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5878 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5878 SmaI CCC'GGG Cuts at: 1872 2944 3093 3205 1872 Size: 1072 149 112 4545 Fragments arranged by size: 4545 1072 149 112 SmlI C'TyrA_G Cuts at: 820 1017 1943 3982 4883 5145 820 Size: 197 926 2039 901 262 1553 Fragments arranged by size: 2039 1553 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 3669 493 Size: 3176 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5878 SphI G_CATG'C Cuts at: 1132 3236 1132 Size: 2104 3774 SspI AAT'ATT Cuts at: 5 52 3802 5 Size: 47 3750 2081 Fragments arranged by size: 3750 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 5878 StyI C'CwwG_G Cuts at: 513 1065 2004 2188 2802 3317 513 Size: 552 939 184 614 515 3074 Fragments arranged by size: 3074 939 614 552 515 184 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2296 2458 3905 3922 4075 4260 4705 Size: 1255 716 162 1447 17 153 185 445 Cuts at: 4705 325 Size: 1498 Fragments arranged by size: 1498 1447 1255 716 445 185 162 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2431 4124 5836 Size: 274 80 33 51 1443 454 1693 1712 Cuts at: 5836 96 Size: 138 Fragments arranged by size: 1712 1693 1443 454 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 5838 5870 5838 Size: 32 5846 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5878 XbaI T'CTAG_A Cuts at: 3214 3214 Size: 5878 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2284 2809 2284 Size: 525 5353 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5878 XmaI C'CCGG_G Cuts at: 1870 2942 3091 3203 1870 Size: 1072 149 112 4545 Fragments arranged by size: 4545 1072 149 112 XmnI GAAnn'nnTTC Cuts at: 2610 4007 2610 Size: 1397 4481 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3686 276 Size: 53 83 186 936 2152 2468 Fragments arranged by size: 2468 2152 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AfeI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PshAI PsiI PstI PvuI PvuII SalI SbfI ScaI SfcI SfiI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AloI ApaI AscI AvrII BaeI BbeI BbvCI BfrBI Bpu10I BsiWI BstZ17I Bsu36I DraIII EcoICRI EcoNI EcoRV FalI FseI FspAI KasI NarI NruI NsiI PacI PmlI PpuMI PspOMI PspXI PsrI RsrII SacI SacII SanDI SapI SexAI SfoI SrfI SwaI