(Circular) (Six-base) MAPSORT of: pFN21AB6256.seq Check: 2272 from: 1 to: 6754 pFN21AB6256 6754 bp With 182 enzymes: SgfI * April 15, 2010 09:24 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3236 4564 278 Size: 53 83 186 936 1700 1328 2468 Fragments arranged by size: 2468 1700 1328 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 4097 1937 Size: 2160 4594 Acc65I G'GTAC_C Cuts at: 4075 4075 Size: 6754 AclI AA'CG_TT Cuts at: 1563 4881 5254 1563 Size: 3318 373 3063 Fragments arranged by size: 3318 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2076 2948 3336 3986 4817 6201 6469 1632 Size: 444 872 388 650 831 1384 268 1917 Fragments arranged by size: 1917 1384 872 831 650 444 388 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 6557 AflIII A'CryG_T Cuts at: 1203 5653 6630 1203 Size: 4450 977 1327 Fragments arranged by size: 4450 1327 977 AgeI A'CCGG_T Cuts at: 5585 5585 Size: 6754 AhdI GACnn_n'nnGTC Cuts at: 5483 5483 Size: 6754 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 6754 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 3284 1184 Size: 2100 4654 AlwNI CAG_nnn'CTG Cuts at: 2447 2638 2903 3157 3766 4497 6069 2447 Size: 191 265 254 609 731 1572 3132 Fragments arranged by size: 3132 1572 731 609 265 254 191 ApaI G_GGCC'C Cuts at: 3524 3524 Size: 6754 ApaLI G'TGCA_C Cuts at: 4811 5967 4811 Size: 1156 5598 ApoI r'AATT_y Cuts at: 1493 3437 4063 4289 4441 6550 1493 Size: 1944 626 226 152 2109 1697 Fragments arranged by size: 2109 1944 1697 626 226 152 AseI AT'TA_AT Cuts at: 160 1032 5308 160 Size: 872 4276 1606 Fragments arranged by size: 4276 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6754 AvaI C'yCGr_G Cuts at: 1870 1943 4079 1870 Size: 73 2136 4545 Fragments arranged by size: 4545 2136 73 BamHI G'GATC_C Cuts at: 4084 4451 4084 Size: 367 6387 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2611 2713 3462 4075 5530 Size: 325 217 627 824 102 749 613 1455 Cuts at: 5530 618 Size: 1842 Fragments arranged by size: 1842 1455 824 749 627 613 325 217 102 BanII G_rGCy'C Cuts at: 1391 2115 2789 2921 3524 4026 4073 1391 Size: 724 674 132 603 502 47 4072 Fragments arranged by size: 4072 724 674 603 502 132 47 BbeI G_GCGC'C Cuts at: 2615 2717 3466 2615 Size: 102 749 5903 Fragments arranged by size: 5903 749 102 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2954 928 Size: 971 1055 4728 Fragments arranged by size: 4728 1055 971 BbvCI CC'TCA_GC Cuts at: 2718 3729 3735 2718 Size: 1011 6 5737 Fragments arranged by size: 5737 1011 6 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 2149 2183 4945 4979 1903 Size: 34 212 34 2762 34 3678 Fragments arranged by size: 3678 2762 212 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3660 4646 5856 3660 Size: 986 1210 4558 Fragments arranged by size: 4558 1210 986 BclI T'GATC_A Cuts at: 1548 3165 1548 Size: 1617 5137 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2609 5365 136 Size: 107 122 71 631 605 937 2756 1525 Fragments arranged by size: 2756 1525 937 631 605 122 107 71 BglII A'GATC_T Cuts at: 6749 6749 Size: 6754 BlpI GC'TnA_GC Cuts at: 4171 4171 Size: 6754 Bme1580I G_kGCm'C Cuts at: 1790 3524 3694 4815 5971 1790 Size: 1734 170 1121 1156 2573 Fragments arranged by size: 2573 1734 1156 1121 170 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 6754 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 4485 5443 442 Size: 416 53 83 399 24 3068 958 1753 Fragments arranged by size: 3068 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 3615 1056 Size: 2559 4195 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2501 2510 2516 2522 2773 2802 2941 3443 Size: 1262 9 6 6 251 29 139 502 Cuts at: 3443 5414 1239 Size: 1971 2579 Fragments arranged by size: 2579 1971 1262 502 251 139 29 9 6 6 Bpu10I CC'TnA_GC Cuts at: 2718 3729 3735 2718 Size: 1011 6 5737 Fragments arranged by size: 5737 1011 6 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3734 3857 4879 5744 6042 3734 Size: 123 1022 865 298 4446 Fragments arranged by size: 4446 1022 865 298 123 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 5417 882 Size: 617 3918 2219 Fragments arranged by size: 3918 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 4545 493 Size: 711 3341 2702 Fragments arranged by size: 3341 2702 711 BsaBI GATnn'nnATC Cuts at: 6748 6748 Size: 6754 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2612 2714 3233 3463 Size: 53 83 186 936 1079 102 519 230 Cuts at: 3463 4561 4943 5578 275 Size: 1098 382 635 1451 Fragments arranged by size: 1451 1098 1079 936 635 519 382 230 186 102 83 53 BsaWI w'CCGG_w Cuts at: 5187 5585 5859 6006 6417 5187 Size: 398 274 147 411 5524 Fragments arranged by size: 5524 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2732 2762 3209 3239 1178 Size: 30 165 30 1329 30 447 30 4693 Fragments arranged by size: 4693 1329 447 165 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 3095 3325 3334 3440 1186 Size: 1909 230 9 106 4500 Fragments arranged by size: 4500 1909 230 106 9 BseYI C'CCAG_C Cuts at: 2156 2185 2323 2511 2625 2655 2724 2778 3099 Size: 29 138 188 114 30 69 54 321 Cuts at: 3099 5957 6453 2156 Size: 2858 496 2457 Fragments arranged by size: 2858 2457 496 321 188 138 114 69 54 30 29 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 3104 3254 1163 Size: 573 1368 150 4663 Fragments arranged by size: 4663 1368 573 150 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 4204 4965 5993 1540 Size: 275 186 2203 761 1028 2301 Fragments arranged by size: 2301 2203 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2789 2921 4815 4900 5971 2789 Size: 132 1894 85 1071 3572 Fragments arranged by size: 3572 1894 1071 132 85 BsmI GAATG_Cn' Cuts at: 2062 4265 4358 2062 Size: 2203 93 4458 Fragments arranged by size: 4458 2203 93 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2115 2789 2921 3524 3694 4026 4073 Size: 399 325 674 132 603 170 332 47 Cuts at: 4073 4815 4900 5971 1391 Size: 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 674 603 399 332 325 170 132 85 47 BspEI T'CCGG_A Cuts at: 6417 6417 Size: 6754 BspHI T'CATG_A Cuts at: 4643 6373 4643 Size: 1730 5024 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3743 4109 844 Size: 2899 366 3489 Fragments arranged by size: 3489 2899 366 BsrBI CCG'CTC Cuts at: 1394 1823 2842 3718 4641 1394 Size: 429 1019 876 923 3507 Fragments arranged by size: 3507 1019 923 876 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2953 5249 5423 65 Size: 1183 1705 2296 174 1396 Fragments arranged by size: 2296 1705 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2300 3706 4000 5398 5585 1700 Size: 110 490 1406 294 1398 187 2869 Fragments arranged by size: 2869 1406 1398 490 294 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 6754 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6754 BssSI C'ACGA_G Cuts at: 3995 4814 5826 3995 Size: 819 1012 4923 Fragments arranged by size: 4923 1012 819 BstAPI GCAn_nnn'nTGC Cuts at: 794 2447 2638 2903 794 Size: 1653 191 265 4645 Fragments arranged by size: 4645 1653 265 191 BstBI TT'CG_AA Cuts at: 5558 5558 Size: 6754 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 6754 BstYI r'GATC_y Cuts at: 1972 3153 3429 3840 4084 4451 4836 4853 6305 Size: 1181 276 411 244 367 385 17 1452 Cuts at: 6305 6391 6403 6749 1972 Size: 86 12 346 1977 Fragments arranged by size: 1977 1452 1181 411 385 367 346 276 244 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 4005 513 Size: 552 2940 3262 Fragments arranged by size: 3262 2940 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 3115 487 Size: 683 56 1889 4126 Fragments arranged by size: 4126 1889 683 56 BtsI GCAGTG_nn' Cuts at: 2399 2474 4270 5064 5084 2399 Size: 75 1796 794 20 4069 Fragments arranged by size: 4069 1796 794 75 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6754 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 3124 3159 529 Size: 35 2560 35 4124 Fragments arranged by size: 4124 2560 35 35 DraI TTT'AAA Cuts at: 3454 4058 3454 Size: 604 6150 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5761 809 Size: 507 4445 1802 Fragments arranged by size: 4445 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2298 3205 3540 Size: 54 1425 50 275 18 468 907 335 Cuts at: 3540 4002 4201 5090 6719 8 Size: 462 199 889 1629 43 Fragments arranged by size: 1629 1425 907 889 468 462 335 275 199 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 4201 1537 Size: 275 2389 4090 Fragments arranged by size: 4090 2389 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2171 3331 3352 4684 1353 Size: 818 1160 21 1332 3423 Fragments arranged by size: 3423 1332 1160 818 21 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 5341 5715 5861 387 Size: 1064 138 3752 374 146 1280 Fragments arranged by size: 3752 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 2787 2919 2787 Size: 132 6622 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2076 2501 2510 2516 2522 2773 2802 Size: 393 444 425 9 6 6 251 29 Cuts at: 2802 2941 2948 3336 3443 3986 4817 5414 6201 Size: 139 7 388 107 543 831 597 787 Cuts at: 6201 6469 1239 Size: 268 1524 Fragments arranged by size: 1524 831 787 597 543 444 425 393 388 268 251 139 107 29 9 7 6 6 EcoNI CCTnn'n_nnAGG Cuts at: 3036 3584 3036 Size: 548 6206 EcoO109I rG'GnC_Cy Cuts at: 2318 3520 3521 3832 2318 Size: 1202 1 311 5240 Fragments arranged by size: 5240 1202 311 1 EcoRI G'AATT_C Cuts at: 4063 4441 4063 Size: 378 6376 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 3281 3281 Size: 6754 FspI TGC'GCA Cuts at: 5260 5260 Size: 6754 HaeII r_GCGC'y Cuts at: 1678 2615 2717 3466 5901 1678 Size: 937 102 749 2435 2531 Fragments arranged by size: 2531 2435 937 749 102 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2379 2411 2994 3019 3026 Size: 32 135 32 807 32 583 25 7 Cuts at: 3026 3051 3472 3489 3504 3521 3543 3575 5395 Size: 25 421 17 15 17 22 32 1820 Cuts at: 5395 5427 5469 5501 1373 Size: 32 42 32 2626 Fragments arranged by size: 2626 1820 807 583 421 135 42 32 32 32 32 32 32 25 25 22 17 17 15 7 HincII GTy'rAC Cuts at: 669 1657 1938 2580 2981 4098 4344 669 Size: 988 281 642 401 1117 246 3079 Fragments arranged by size: 3079 1117 988 642 401 281 246 HindIII A'AGCT_T Cuts at: 748 3447 748 Size: 2699 4055 HpaI GTT'AAC Cuts at: 4344 4344 Size: 6754 KasI G'GCGC_C Cuts at: 2611 2713 3462 2611 Size: 102 749 5903 Fragments arranged by size: 5903 749 102 KpnI G_GTAC'C Cuts at: 4079 4079 Size: 6754 MfeI C'AATT_G Cuts at: 4353 4353 Size: 6754 MluI A'CGCG_T Cuts at: 6630 6630 Size: 6754 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2798 5868 6052 1305 Size: 1493 3070 184 2007 Fragments arranged by size: 3070 2007 1493 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 4932 Fragments arranged by size: 4932 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 4712 5071 5230 518 Size: 648 3546 359 159 2042 Fragments arranged by size: 3546 2042 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2447 2638 2704 2866 2900 3398 4170 4847 Size: 841 191 66 162 34 498 772 677 Cuts at: 4847 5995 6240 1606 Size: 1148 245 2120 Fragments arranged by size: 2120 1148 841 772 677 498 245 191 162 66 34 NaeI GCC'GGC Cuts at: 2302 4002 2302 Size: 1700 5054 NarI GG'CG_CC Cuts at: 2612 2714 3463 2612 Size: 102 749 5903 Fragments arranged by size: 5903 749 102 NcoI C'CATG_G Cuts at: 513 1065 513 Size: 552 6202 NdeI CA'TA_TG Cuts at: 387 387 Size: 6754 NgoMIV G'CCGG_C Cuts at: 2300 4000 2300 Size: 1700 5054 NheI G'CTAG_C Cuts at: 1052 3611 1052 Size: 2559 4195 NotI GC'GGCC_GC Cuts at: 4201 4201 Size: 6754 NspI r_CATG'y Cuts at: 1132 1229 4112 5657 1132 Size: 97 2883 1545 2229 Fragments arranged by size: 2883 2229 1545 97 PasI CC'CwG_GG Cuts at: 1348 3118 3175 1348 Size: 1770 57 4927 Fragments arranged by size: 4927 1770 57 PciI A'CATG_T Cuts at: 5653 5653 Size: 6754 PflMI CCAn_nnn'nTGG Cuts at: 1108 2434 2825 1108 Size: 1326 391 5037 Fragments arranged by size: 5037 1326 391 PfoI T'CCnGG_A Cuts at: 2818 2818 Size: 6754 PmeI GTTT'AAAC Cuts at: 4058 4058 Size: 6754 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4823 4855 6336 6368 4823 Size: 32 1481 32 5209 Fragments arranged by size: 5209 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6754 PsiI TTA'TAA Cuts at: 4324 4324 Size: 6754 PspOMI G'GGCC_C Cuts at: 3520 3520 Size: 6754 PstI C_TGCA'G Cuts at: 830 2452 2643 2899 2908 3551 4106 830 Size: 1622 191 256 9 643 555 3478 Fragments arranged by size: 3478 1622 643 555 256 191 9 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6754 PvuII CAG'CTG Cuts at: 2447 2704 2866 2447 Size: 257 162 6335 Fragments arranged by size: 6335 257 162 SacI G_AGCT'C Cuts at: 2789 2921 2789 Size: 132 6622 SalI G'TCGA_C Cuts at: 1936 4096 1936 Size: 2160 4594 SapI GCTCTTCn'nnn_ Cuts at: 2171 2171 Size: 6754 SbfI CC_TGCA'GG Cuts at: 4106 4106 Size: 6754 ScaI AGT'ACT Cuts at: 5002 6714 5002 Size: 1712 5042 SfcI C'TryA_G Cuts at: 826 1045 2448 2639 2861 2895 2904 3513 3547 Size: 219 1403 191 222 34 9 609 34 Cuts at: 3547 3606 4102 5237 5918 6109 826 Size: 59 496 1135 681 191 1471 Fragments arranged by size: 1471 1403 1135 681 609 496 222 219 191 191 59 34 34 9 SfoI GGC'GCC Cuts at: 2613 2715 3464 2613 Size: 102 749 5903 Fragments arranged by size: 5903 749 102 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6754 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6754 SmaI CCC'GGG Cuts at: 1872 4081 1872 Size: 2209 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 3713 3836 4858 5759 6021 820 Size: 197 926 1770 123 1022 901 262 1553 Fragments arranged by size: 1770 1553 1022 926 901 262 197 123 SnaBI TAC'GTA Cuts at: 493 4545 493 Size: 4052 2702 SpeI A'CTAG_T Cuts at: 152 2983 152 Size: 2831 3923 SphI G_CATG'C Cuts at: 1132 4112 1132 Size: 2980 3774 SspI AAT'ATT Cuts at: 5 52 4678 5 Size: 47 4626 2081 Fragments arranged by size: 4626 2081 47 StuI AGG'CCT Cuts at: 1517 2939 1517 Size: 1422 5332 StyI C'CwwG_G Cuts at: 513 1065 2428 3256 3472 4193 513 Size: 552 1363 828 216 721 3074 Fragments arranged by size: 3074 1363 828 721 552 216 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2866 3188 3208 3785 3908 3936 4781 Size: 1255 1286 322 20 577 123 28 845 Cuts at: 4781 4798 4951 5136 5581 325 Size: 17 153 185 445 1498 Fragments arranged by size: 1498 1286 1255 845 577 445 322 185 153 123 28 20 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 5000 6712 96 Size: 274 80 33 51 1443 3023 1712 138 Fragments arranged by size: 3023 1712 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2732 2764 3209 3241 6714 6746 2732 Size: 32 445 32 3473 32 2740 Fragments arranged by size: 3473 2740 445 32 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 6754 XbaI T'CTAG_A Cuts at: 4090 4090 Size: 6754 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6754 XmaI C'CCGG_G Cuts at: 1870 4079 1870 Size: 2209 4545 XmnI GAAnn'nnTTC Cuts at: 4883 4883 Size: 6754 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3234 4562 276 Size: 53 83 186 936 1700 1328 2468 Fragments arranged by size: 2468 1700 1328 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PshAI PsiI PspOMI PstI PvuI PvuII SacI SalI SapI SbfI ScaI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI AscI AvrII BaeI BfrBI BplI BsiWI BsmBI BstXI BstZ17I Bsu36I DraIII EcoRV FseI FspAI NruI NsiI PacI PmlI PpuMI PspXI PsrI RsrII SacII SanDI SexAI SfiI SrfI SwaI XcmI