Restriction map for
pFN21AE4161
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# NO CUT ENZYMES ( 105 enzymes) #
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AatII Acc65I AccI AccIII AclI
AcsI AflII AgeI AhdI Aor51HI
ApoI AscI AseI AsnI Asp700I
Asp718I AspEI AspI AvrII BamHI
BbsI BcgI BciVI BclI BfrI
BlnI BlpI Bpu1102I BpuAI BsaBI
BseAI BsgI BsiWI BsmBI BsmI
Bsp1407I BspDI BspEI BspHI BsrGI
BssSI Bst1107I BstBI BstEII BstPI
BstZ17I CelII ClaI DraI DraIII
DrdI Eam1105I Eco47III EcoO65I EcoRI
EcoRV EcoT22I FbaI HincII HindII
HindIII HpaI KpnI MamI MfeI
MluI MroI MseI MunI NdeI
NheI NotI NruI NsiI NspV
PacI PaeR7I PflFI PinAI PmeI
PshAI PshBI Psp1406I PvuI RcaI
SacI SalI SbfI ScaI SexAI
SfuI SgrAI SnaBI SpeI SphI
Sse8387I SspBI SspI SwaI Tru9I
Tsp509I Tth111I XbaI XhoI XmnI
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# ONE CUT ENZYMES ( 29 enzymes) #
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AcyI 1047 GGCGCC G[GA]^CG[CT]C
AocI 1030 CCTGAGG CC^T[ACGT]AGG
AviII 776 TGCGCA TGC^GCA
BbeI 1047 GGCGCC GGCGC^C
BfaI 1369 CTAG C^TAG
BglII 1448 AGATCT A^GATCT
BsaHI 1047 GGCGCC G[GA]^CG[CT]C
BsaI 1562 GAGACC GGTCTC(1/5)
BsaWI 655 TCCGGT [AT]^CCGG[AT]
BspLU11I 913 ACATGT A^CATGT
BsrDI 1766 GCAATG GCAATG(2/0)
Bsu36I 1030 CCTGAGG CC^T[ACGT]AGG
CpoI 652 CGGTCCG CG^G[AT]CCG
Eco81I 1030 CCTGAGG CC^T[ACGT]AGG
FseI 1788 GGCCGGCC GGCCGG^CC
FspI 776 TGCGCA TGC^GCA
HgaI 1081 GCGTC GACGC(5/10)
Hin1I 1047 GGCGCC G[GA]^CG[CT]C
KasI 1047 GGCGCC G^GCGCC
KspI 1239 CCGCGG CCGC^GG
MaeI 1369 CTAG C^TAG
MslI 319 CACCAGTGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
NarI 1047 GGCGCC GG^CGCC
NcoI 141 CCATGG C^CATGG
PvuII 227 CAGCTG CAG^CTG
RsrII 652 CGGTCCG CG^G[AT]CCG
SacII 1239 CCGCGG CCGC^GG
SfoI 1047 GGCGCC GGC^GCC
TfiI 1386 GATTC G^A[AT]TC
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# TWO CUT ENZYMES ( 22 enzymes) #
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AfaI 1344 GTAC GT^AC
1413 GTAC
Alw44I 815 GTGCAC G^TGCAC
1491 GTGCAC
ApaLI 815 GTGCAC G^TGCAC
1491 GTGCAC
BalI 179 TGGCCA TGG^CCA
980 TGGCCA
BbvCI 482 CCTCAGC CCTCAGC(-5/-2)
466 GCTGAGG
BssHII 1470 GCGCGC G^CGCGC
1472 GCGCGC
BstAPI 223 GCAGCAGCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
1589 GCAAACAGTGC
EarI 1222 CTCTTC CTCTTC(1/4)
745 GAAGAG
EcoNI 433 CCTGACCCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
735 CCTCCGCAAGG
Ksp632I 1222 CTCTTC CTCTTC(1/4)
745 GAAGAG
MluNI 179 TGGCCA TGG^CCA
980 TGGCCA
MscI 179 TGGCCA TGG^CCA
980 TGGCCA
NaeI 204 GCCGGC GCC^GGC
1789 GCCGGC
NgoMIV 204 GCCGGC G^CCGGC
1789 GCCGGC
NspI 913 ACATGT [GA]CATG^[CT]
1415 ACATGC
PpuMI 188 AGGTCCC [GA]G^G[AT]CC[CT]
882 AGGTCCT
PstI 559 CTGCAG CTGCA^G
669 CTGCAG
RsaI 1344 GTAC GT^AC
1413 GTAC
SapI 745 GAAGAGC GCTCTTC(1/4)
1221 GCTCTTC
SmlI 94 CTCAAG C^T[CT][GA]AG
1486 CTCAAG
StuI 853 AGGCCT AGG^CCT
1027 AGGCCT
XcmI 318 CCACCAGTGTGGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
1466 CCACGCGCGCGCTGG
#########################################
# 3 OR MORE CUT ENZYMES ( 102 enzymes) #
#########################################
AccII AciI AflIII AluI AlwI
AlwNI ApaI AspHI AvaI AvaII
BanI BanII BbrPI BbvI BcnI
BglI BmyI BpmI BsaAI BsaJI
BseRI BsiEI BsiHKAI BsiYI BslI
BsmAI BsmFI BsoBI Bsp1286I BspMI
BsrBI BsrFI BsrI BssKI BstF5I
BstNI BstUI BstXI BstYI Cac8I
CfoI Cfr10I Cfr13I DdeI DpnII
DraII DsaI EaeI EagI EclXI
Eco52I EcoO109I EcoRII EcoT14I FauI
Fnu4HI FokI HaeII HaeIII HapII
HhaI HinP1I HinfI HpaII HphI
ItaI MaeII MaeIII MboI MboII
MflI MnlI MspA1I MspI MvaI
MvnI MwoI NciI NdeII NlaIII
NlaIV PflMI PleI PmaCI PmlI
Sau3AI Sau96I ScrFI SfaNI SfcI
SfiI SmaI StyI TaqI TseI
Tsp45I TspRI TthHB8I Van91I XhoII
XmaCI XmaI