(Circular) (Six-base) MAPSORT of: pFN21AE4161.seq Check: 7999 from: 1 to: 6514 pFN21AE4161 6514 bp With 182 enzymes: SgfI * July 6, 2012 17:34 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 3509 3509 Size: 6514 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 4324 278 Size: 53 83 186 936 2788 2468 Fragments arranged by size: 2788 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3857 1937 Size: 1920 4594 Acc65I G'GTAC_C Cuts at: 3835 3835 Size: 6514 AclI AA'CG_TT Cuts at: 1563 4641 5014 1563 Size: 3078 373 3063 Fragments arranged by size: 3078 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 3442 4577 5961 6229 1632 Size: 1810 1135 1384 268 1917 Fragments arranged by size: 1917 1810 1384 1135 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 6317 AflIII A'CryG_T Cuts at: 1203 2265 2918 3357 3627 5413 6390 1203 Size: 1062 653 439 270 1786 977 1327 Fragments arranged by size: 1786 1327 1062 977 653 439 270 AgeI A'CCGG_T Cuts at: 5345 5345 Size: 6514 AhdI GACnn_n'nnGTC Cuts at: 5243 5243 Size: 6514 AleI CACnn'nnGTG Cuts at: 1166 2328 1166 Size: 1162 5352 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 6514 AlwNI CAG_nnn'CTG Cuts at: 2234 2237 2597 2771 2891 3400 4257 5829 2234 Size: 3 360 174 120 509 857 1572 2919 Fragments arranged by size: 2919 1572 857 509 360 174 120 3 ApaI G_GGCC'C Cuts at: 2462 2483 2899 3261 2462 Size: 21 416 362 5715 Fragments arranged by size: 5715 416 362 21 ApaLI G'TGCA_C Cuts at: 2820 3496 4571 5727 2820 Size: 676 1075 1156 3607 Fragments arranged by size: 3607 1156 1075 676 ApoI r'AATT_y Cuts at: 1493 3823 4049 4201 6310 1493 Size: 2330 226 152 2109 1697 Fragments arranged by size: 2330 2109 1697 226 152 AseI AT'TA_AT Cuts at: 160 1032 5068 160 Size: 872 4036 1606 Fragments arranged by size: 4036 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6514 AvaI C'yCGr_G Cuts at: 1870 1943 2285 2759 2898 3072 3434 3839 1870 Size: 73 342 474 139 174 362 405 4545 Fragments arranged by size: 4545 474 405 362 342 174 139 73 BamHI G'GATC_C Cuts at: 3844 4211 3844 Size: 367 6147 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2040 2057 3022 3052 3155 Size: 325 217 627 253 17 965 30 103 Cuts at: 3155 3645 3835 5290 618 Size: 490 190 1455 1842 Fragments arranged by size: 1842 1455 965 627 490 325 253 217 190 103 30 17 BanII G_rGCy'C Cuts at: 1391 2462 2483 2817 2899 3261 3833 1391 Size: 1071 21 334 82 362 572 4072 Fragments arranged by size: 4072 1071 572 362 334 82 21 BbeI G_GCGC'C Cuts at: 3056 3056 Size: 6514 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 5543 BbvCI CC'TCA_GC Cuts at: 2472 2488 2472 Size: 16 6498 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4705 4739 1903 Size: 34 2768 34 3678 Fragments arranged by size: 3678 2768 34 34 BciVI GTATCCnnnnn_n' Cuts at: 4406 5616 4406 Size: 1210 5304 BclI T'GATC_A Cuts at: 1548 1548 Size: 6514 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2457 3256 3738 Size: 107 122 71 631 605 785 799 482 Cuts at: 3738 3795 5125 136 Size: 57 1330 1525 Fragments arranged by size: 1525 1330 799 785 631 605 482 122 107 71 57 BglII A'GATC_T Cuts at: 3453 6509 3453 Size: 3056 3458 BlpI GC'TnA_GC Cuts at: 3931 3931 Size: 6514 Bme1580I G_kGCm'C Cuts at: 1790 2045 2373 2462 2483 2824 2899 3261 3323 Size: 255 328 89 21 341 75 362 62 Cuts at: 3323 3500 4575 5731 1790 Size: 177 1075 1156 2573 Fragments arranged by size: 2573 1156 1075 362 341 328 255 177 89 75 62 21 BmgBI CAC'GTC Cuts at: 1318 2503 3106 3628 1318 Size: 1185 603 522 4204 Fragments arranged by size: 4204 1185 603 522 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2383 2835 3766 Size: 416 53 83 399 24 966 452 931 Cuts at: 3766 4245 5203 442 Size: 479 958 1753 Fragments arranged by size: 1753 966 958 931 479 452 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6514 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3590 3590 Size: 6514 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 3221 3425 3580 5174 1239 Size: 1982 204 155 1594 2579 Fragments arranged by size: 2579 1982 1594 204 155 Bpu10I CC'TnA_GC Cuts at: 2118 2472 2488 2118 Size: 354 16 6144 Fragments arranged by size: 6144 354 16 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2084 3476 4639 5504 5802 2084 Size: 1392 1163 865 298 2796 Fragments arranged by size: 2796 1392 1163 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 3561 5177 882 Size: 617 2062 1616 2219 Fragments arranged by size: 2219 2062 1616 617 BsaAI yAC'GTr Cuts at: 493 1204 2268 3100 3358 3442 4305 493 Size: 711 1064 832 258 84 863 2702 Fragments arranged by size: 2702 1064 863 832 711 258 84 BsaBI GATnn'nnATC Cuts at: 6508 6508 Size: 6514 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3053 4321 4703 5338 Size: 53 83 186 936 1520 1268 382 635 Cuts at: 5338 275 Size: 1451 Fragments arranged by size: 1520 1451 1268 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 2660 4947 5345 5619 5766 6177 2660 Size: 2287 398 274 147 411 2997 Fragments arranged by size: 2997 2287 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2398 2416 2428 2446 3167 Size: 30 165 30 995 18 12 18 721 Cuts at: 3167 3197 1178 Size: 30 4495 Fragments arranged by size: 4495 995 721 165 30 30 30 18 18 12 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2210 2424 2489 3188 3818 1186 Size: 1024 214 65 699 630 3882 Fragments arranged by size: 3882 1024 699 630 214 65 BseYI C'CCAG_C Cuts at: 2205 2590 2963 5717 6213 2205 Size: 385 373 2754 496 2506 Fragments arranged by size: 2754 2506 496 385 373 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 1163 Size: 573 5941 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2582 2809 3279 3715 3799 3964 Size: 275 186 581 227 470 436 84 165 Cuts at: 3964 4725 5753 1540 Size: 761 1028 2301 Fragments arranged by size: 2301 1028 761 581 470 436 275 227 186 165 84 BsiHKAI G_wGCw'C Cuts at: 2824 3441 3500 4575 4660 5731 2824 Size: 617 59 1075 85 1071 3607 Fragments arranged by size: 3607 1075 1071 617 85 59 BsmI GAATG_Cn' Cuts at: 4025 4118 4025 Size: 93 6421 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2045 2373 2462 2483 2817 2824 2899 Size: 399 255 328 89 21 334 7 75 Cuts at: 2899 3261 3323 3441 3500 3833 4575 4660 5731 Size: 362 62 118 59 333 742 85 1071 Cuts at: 5731 1391 Size: 2174 Fragments arranged by size: 2174 1071 742 399 362 334 333 328 255 118 89 85 75 62 59 21 7 BspEI T'CCGG_A Cuts at: 6177 6177 Size: 6514 BspHI T'CATG_A Cuts at: 4403 6133 4403 Size: 1730 4784 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2517 3509 3678 3869 844 Size: 1673 992 169 191 3489 Fragments arranged by size: 3489 1673 992 191 169 BsrBI CCG'CTC Cuts at: 1394 1823 2218 2755 3574 4401 1394 Size: 429 395 537 819 827 3507 Fragments arranged by size: 3507 827 819 537 429 395 BsrDI GCAATG_nn' Cuts at: 65 1248 3778 5009 5183 65 Size: 1183 2530 1231 174 1396 Fragments arranged by size: 2530 1396 1231 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2209 2492 2629 3351 3794 5158 5345 Size: 110 399 283 137 722 443 1364 187 Cuts at: 5345 1700 Size: 2869 Fragments arranged by size: 2869 1364 722 443 399 283 187 137 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 6514 BssHII G'CGCG_C Cuts at: 1925 3475 3477 1925 Size: 1550 2 4962 Fragments arranged by size: 4962 1550 2 BssSI C'ACGA_G Cuts at: 4574 5586 4574 Size: 1012 5502 BstAPI GCAn_nnn'nTGC Cuts at: 794 2234 3600 794 Size: 1440 1366 3708 Fragments arranged by size: 3708 1440 1366 BstBI TT'CG_AA Cuts at: 5318 5318 Size: 6514 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 6514 BstXI CCAn_nnnn'nTGG Cuts at: 2330 2333 2641 2971 2330 Size: 3 308 330 5873 Fragments arranged by size: 5873 330 308 3 BstYI r'GATC_y Cuts at: 1972 2645 3378 3453 3844 4211 4596 4613 6065 Size: 673 733 75 391 367 385 17 1452 Cuts at: 6065 6151 6163 6509 1972 Size: 86 12 346 1977 Fragments arranged by size: 1977 1452 733 673 391 385 367 346 86 75 17 12 Bsu36I CC'TnA_GG Cuts at: 3036 3036 Size: 6514 BtgI C'CryG_G Cuts at: 513 1065 2004 2011 2146 2694 3244 513 Size: 552 939 7 135 548 550 3783 Fragments arranged by size: 3783 939 552 550 548 135 7 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 2770 3597 487 Size: 683 56 1544 827 3404 Fragments arranged by size: 3404 1544 827 683 56 BtsI GCAGTG_nn' Cuts at: 3291 4030 4824 4844 3291 Size: 739 794 20 4961 Fragments arranged by size: 4961 794 739 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6514 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 2163 2198 529 Size: 35 1599 35 4845 Fragments arranged by size: 4845 1599 35 35 DraI TTT'AAA Cuts at: 3818 3818 Size: 6514 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5521 809 Size: 507 4205 1802 Fragments arranged by size: 4205 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2184 2520 2631 Size: 54 1425 50 275 18 354 336 111 Cuts at: 2631 2806 2985 3276 3288 3483 3796 3961 4850 Size: 175 179 291 12 195 313 165 889 Cuts at: 4850 6479 8 Size: 1629 43 Fragments arranged by size: 1629 1425 889 354 336 313 291 275 195 179 175 165 111 54 50 43 18 12 EagI C'GGCC_G Cuts at: 1537 1812 2806 3276 3796 3961 1537 Size: 275 994 470 520 165 4090 Fragments arranged by size: 4090 994 520 470 275 165 EarI CTCTTCn'nnn_ Cuts at: 1353 2745 3233 4444 1353 Size: 1392 488 1211 3423 Fragments arranged by size: 3423 1392 1211 488 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2419 2602 3008 3051 3341 5101 Size: 1064 138 830 183 406 43 290 1760 Cuts at: 5101 5475 5621 387 Size: 374 146 1280 Fragments arranged by size: 1760 1280 1064 830 406 374 290 183 146 138 43 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 3221 3425 3442 3580 4577 5174 5961 Size: 393 1589 204 17 138 997 597 787 Cuts at: 5961 6229 1239 Size: 268 1524 Fragments arranged by size: 1589 1524 997 787 597 393 268 204 138 17 EcoNI CCTnn'n_nnAGG Cuts at: 2442 2744 2442 Size: 302 6212 EcoO109I rG'GnC_Cy Cuts at: 2194 2458 2459 2479 2480 2737 2768 2888 2895 Size: 264 1 20 1 257 31 120 7 Cuts at: 2895 3258 2194 Size: 363 5450 Fragments arranged by size: 5450 363 264 257 120 31 20 7 1 1 EcoRI G'AATT_C Cuts at: 3823 4201 3823 Size: 378 6136 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2326 2326 Size: 6514 FseI GG_CCGG'CC Cuts at: 3798 3798 Size: 6514 FspI TGC'GCA Cuts at: 2783 5020 2783 Size: 2237 4277 FspAI rTGC'GCAy Cuts at: 2783 2783 Size: 6514 HaeII r_GCGC'y Cuts at: 1678 2413 2597 3056 5661 1678 Size: 735 184 459 2605 2531 Fragments arranged by size: 2605 2531 735 459 184 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2398 2430 2498 2530 5155 Size: 32 135 32 826 32 68 32 2625 Cuts at: 5155 5187 5229 5261 1373 Size: 32 42 32 2626 Fragments arranged by size: 2626 2625 826 135 68 42 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3858 4104 669 Size: 988 281 1920 246 3079 Fragments arranged by size: 3079 1920 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 6514 HpaI GTT'AAC Cuts at: 4104 4104 Size: 6514 KasI G'GCGC_C Cuts at: 3052 3052 Size: 6514 KpnI G_GTAC'C Cuts at: 3839 3839 Size: 6514 MfeI C'AATT_G Cuts at: 4113 4113 Size: 6514 MluI A'CGCG_T Cuts at: 6390 6390 Size: 6514 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2826 5628 5812 1305 Size: 1521 2802 184 2007 Fragments arranged by size: 2802 2007 1521 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2186 2987 10 Size: 54 1425 343 354 801 3537 Fragments arranged by size: 3537 1425 801 354 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2328 4472 4831 4990 518 Size: 648 1162 2144 359 159 2042 Fragments arranged by size: 2144 2042 1162 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2234 2548 2832 3246 3397 3642 3930 4607 Size: 628 314 284 414 151 245 288 677 Cuts at: 4607 5755 6000 1606 Size: 1148 245 2120 Fragments arranged by size: 2120 1148 677 628 414 314 288 284 245 245 151 NaeI GCC'GGC Cuts at: 2211 3796 2211 Size: 1585 4929 NarI GG'CG_CC Cuts at: 3053 3053 Size: 6514 NcoI C'CATG_G Cuts at: 513 1065 2004 2146 513 Size: 552 939 142 4881 Fragments arranged by size: 4881 939 552 142 NdeI CA'TA_TG Cuts at: 387 387 Size: 6514 NgoMIV G'CCGG_C Cuts at: 2209 3794 2209 Size: 1585 4929 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6514 NotI GC'GGCC_GC Cuts at: 3961 3961 Size: 6514 NspI r_CATG'y Cuts at: 1132 1229 2922 3424 3872 5417 1132 Size: 97 1693 502 448 1545 2229 Fragments arranged by size: 2229 1693 1545 502 448 97 PasI CC'CwG_GG Cuts at: 1348 2444 2771 1348 Size: 1096 327 5091 Fragments arranged by size: 5091 1096 327 PciI A'CATG_T Cuts at: 2918 5413 2918 Size: 2495 4019 PflMI CCAn_nnn'nTGG Cuts at: 1108 2147 2771 2891 1108 Size: 1039 624 120 4731 Fragments arranged by size: 4731 1039 624 120 PfoI T'CCnGG_A Cuts at: 2259 2259 Size: 6514 PmeI GTTT'AAAC Cuts at: 3818 3818 Size: 6514 PmlI CAC'GTG Cuts at: 2268 3100 3358 3442 2268 Size: 832 258 84 5340 Fragments arranged by size: 5340 832 258 84 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4583 4615 6096 6128 4583 Size: 32 1481 32 4969 Fragments arranged by size: 4969 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2194 2888 2194 Size: 694 5820 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6514 PsiI TTA'TAA Cuts at: 4084 4084 Size: 6514 PspOMI G'GGCC_C Cuts at: 2458 2479 2895 3257 2458 Size: 21 416 362 5715 Fragments arranged by size: 5715 416 362 21 PstI C_TGCA'G Cuts at: 830 2568 2678 3866 830 Size: 1738 110 1188 3478 Fragments arranged by size: 3478 1738 1188 110 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6514 PvuII CAG'CTG Cuts at: 2234 2234 Size: 6514 RsrII CG'GwC_CG Cuts at: 2658 2658 Size: 6514 SacII CC_GC'GG Cuts at: 3247 3247 Size: 6514 SalI G'TCGA_C Cuts at: 1936 3856 1936 Size: 1920 4594 SapI GCTCTTCn'nnn_ Cuts at: 2745 3233 2745 Size: 488 6026 SbfI CC_TGCA'GG Cuts at: 3866 3866 Size: 6514 ScaI AGT'ACT Cuts at: 4762 6474 4762 Size: 1712 4802 SfcI C'TryA_G Cuts at: 826 1045 2564 2598 2674 3862 4997 5678 5869 Size: 219 1519 34 76 1188 1135 681 191 Cuts at: 5869 826 Size: 1471 Fragments arranged by size: 1519 1471 1188 1135 681 219 191 76 34 SfiI GGCCn_nnn'nGGCC Cuts at: 2457 3256 3795 2457 Size: 799 539 5176 Fragments arranged by size: 5176 799 539 SfoI GGC'GCC Cuts at: 3054 3054 Size: 6514 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6514 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6514 SmaI CCC'GGG Cuts at: 1872 2287 2761 3074 3841 1872 Size: 415 474 313 767 4545 Fragments arranged by size: 4545 767 474 415 313 SmlI C'TyrA_G Cuts at: 820 1017 1943 2099 3491 4618 5519 5781 820 Size: 197 926 156 1392 1127 901 262 1553 Fragments arranged by size: 1553 1392 1127 926 901 262 197 156 SnaBI TAC'GTA Cuts at: 493 4305 493 Size: 3812 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6514 SphI G_CATG'C Cuts at: 1132 3872 1132 Size: 2740 3774 SrfI GCCC'GGGC Cuts at: 2761 2761 Size: 6514 SspI AAT'ATT Cuts at: 5 52 4438 5 Size: 47 4386 2081 Fragments arranged by size: 4386 2081 47 StuI AGG'CCT Cuts at: 1517 2860 3034 1517 Size: 1343 174 4997 Fragments arranged by size: 4997 1343 174 StyI C'CwwG_G Cuts at: 513 1065 2004 2146 2181 2391 2427 2634 2733 Size: 552 939 142 35 210 36 207 99 Cuts at: 2733 2904 3953 513 Size: 171 1049 3074 Fragments arranged by size: 3074 1049 939 552 210 207 171 142 99 36 35 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2596 3748 4541 4558 4711 4896 5341 Size: 1255 1016 1152 793 17 153 185 445 Cuts at: 5341 325 Size: 1498 Fragments arranged by size: 1498 1255 1152 1016 793 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 3417 4760 6472 Size: 274 80 33 51 1443 1440 1343 1712 Cuts at: 6472 96 Size: 138 Fragments arranged by size: 1712 1443 1440 1343 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2416 2448 3167 3199 6474 6506 2416 Size: 32 719 32 3275 32 2424 Fragments arranged by size: 3275 2424 719 32 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 6514 XbaI T'CTAG_A Cuts at: 3850 3850 Size: 6514 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2330 3478 2330 Size: 1148 5366 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6514 XmaI C'CCGG_G Cuts at: 1870 2285 2759 3072 3839 1870 Size: 415 474 313 767 4545 Fragments arranged by size: 4545 767 474 415 313 XmnI GAAnn'nnTTC Cuts at: 4643 4643 Size: 6514 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 4322 276 Size: 53 83 186 936 2788 2468 Fragments arranged by size: 2788 2468 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoNI EcoO109I EcoRI FalI FseI FspI FspAI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PfoI PmeI PmlI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI PvuII RsrII SacII SalI SapI SbfI ScaI SfcI SfiI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SrfI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI AloI AscI AvrII BaeI BfrBI BsiWI BsmBI BstZ17I DraIII EcoICRI EcoRV NruI NsiI PacI PspXI PsrI SacI SanDI SexAI SwaI