Restriction map for
pFN21AB5934
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# NO CUT ENZYMES ( 130 enzymes) #
##################################
AatII Acc65I AccIII AclI AfaI
AflII AgeI AhdI Alw44I AocI
ApaI ApaLI AscI AseI AsnI
Asp718I AspEI AspI AvaI AviII
AvrII BalI BbrPI BciVI BclI
BfrI BglI BlnI BlpI Bpu1102I
BsaAI BsaBI BsaWI BseAI BsiEI
BsiWI BsmBI BsmI BsoBI Bsp1407I
BspDI BspEI BspHI BsrGI BssHII
BssSI Bst1107I BstBI BstEII BstPI
BstZ17I Bsu36I CelII ClaI CpoI
DraI DraIII DrdI EaeI EagI
Eam1105I EclXI Eco52I Eco81I EcoNI
EcoO65I EcoRI EcoT22I FbaI FseI
FspI HgaI HindIII HpaI KpnI
KspI MaeII MamI MfeI MluI
MluNI MroI MscI MunI NheI
NotI NruI NsiI NspV PacI
PaeR7I PflFI PflMI PinAI PmaCI
PmeI PmlI PshAI PshBI Psp1406I
PvuI RcaI RsaI RsrII SacII
SalI SapI SbfI ScaI SexAI
SfiI SfuI SgrAI SmaI SnaBI
SpeI SphI Sse8387I SspBI SspI
SwaI TaqI Tth111I TthHB8I Van91I
XbaI XcmI XhoI XmaCI XmaI
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# ONE CUT ENZYMES ( 42 enzymes) #
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AccI 42 GTCTAC GT^[AC][GT]AC
AcsI 1556 GAATTT [GA]^AATT[CT]
AcyI 393 GGCGCC G[GA]^CG[CT]C
AflIII 868 ACATGT A^C[GA][CT]GT
Aor51HI 140 AGCGCT AGC^GCT
ApoI 1556 GAATTT [GA]^AATT[CT]
Asp700I 1212 GAAGACCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AvaII 558 GGTCC G^G[AT]CC
BanI 393 GGCGCC G^G[CT][GA]CC
BbeI 393 GGCGCC GGCGC^C
BbvCI 796 GCTGAGG CCTCAGC(-5/-2)
BcgI 202 CGAGAGCTCTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BglII 114 AGATCT A^GATCT
BsaHI 393 GGCGCC G[GA]^CG[CT]C
BsaI 747 GGTCTC GGTCTC(1/5)
BspLU11I 868 ACATGT A^CATGT
BspMI 399 GCAGGT ACCTGC(4/8)
BsrBI 336 GAGCGG CCGCTC(-3/-3)
BsrDI 852 CATTGC GCAATG(2/0)
BstAPI 423 GCAAGAACTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstXI 948 CCATGAATGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
EarI 1033 GAAGAG CTCTTC(1/4)
Eco47III 140 AGCGCT AGC^GCT
EcoRV 828 GATATC GAT^ATC
Hin1I 393 GGCGCC G[GA]^CG[CT]C
HincII 412 GTTGAC GT[CT]^[GA]AC
HindII 412 GTTGAC GT[CT]^[GA]AC
KasI 393 GGCGCC G^GCGCC
Ksp632I 1033 GAAGAG CTCTTC(1/4)
MaeIII 471 GTCAC ^GT[ACGT]AC
MseI 13 TTAA T^TAA
NaeI 390 GCCGGC GCC^GGC
NarI 393 GGCGCC GG^CGCC
NdeI 1106 CATATG CA^TATG
NgoMIV 390 GCCGGC G^CCGGC
NspI 868 ACATGT [GA]CATG^[CT]
PpuMI 557 AGGTCCC [GA]G^G[AT]CC[CT]
SfoI 393 GGCGCC GGC^GCC
SmlI 508 CTCAAG C^T[CT][GA]AG
Tru9I 13 TTAA T^TAA
Tsp45I 471 GTCAC ^GT[GC]AC
XmnI 1212 GAAGACCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 24 enzymes) #
###################################
AccII 136 CGCG CG^CG
181 CGCG
AspHI 205 GAGCTC G[AT]GC[AT]^C
1059 GAGCTC
BamHI 526 GGATCC G^GATCC
1337 GGATCC
BcnI 162 CCCGG CC^[GC]GG
295 CCGGG
BfaI 5 CTAG C^TAG
611 CTAG
BseRI 343 GAGGAG GAGGAG(10/8)
799 GAGGAG
BsgI 872 GTGCAG GTGCAG(16/14)
1281 GTGCAG
BsiHKAI 205 GAGCTC G[AT]GC[AT]^C
1059 GAGCTC
BsmAI 1326 GAGAC GTCTC(1/5)
748 GTCTC
BsrFI 94 GCCGGT [GA]^CCGG[CT]
390 GCCGGC
BstUI 136 CGCG CG^CG
181 CGCG
Cfr10I 94 GCCGGT [GA]^CCGG[CT]
390 GCCGGC
DraII 174 AGGCCCC [GA]G^G[ACGT]CC[CT]
557 AGGTCCC
DsaI 573 CCATGG C^C[GA][CT]GG
735 CCATGG
EcoO109I 174 AGGCCCC [GA]G^G[ACGT]CC[CT]
557 AGGTCCC
EcoT14I 573 CCATGG C^C[AT][AT]GG
735 CCATGG
MaeI 5 CTAG C^TAG
611 CTAG
MspA1I 379 CAGCTG C[AC]G^C[GT]G
794 CAGCTG
MvnI 136 CGCG CG^CG
181 CGCG
NciI 162 CCCGG CC^[GC]GG
295 CCGGG
NcoI 573 CCATGG C^CATGG
735 CCATGG
PvuII 379 CAGCTG CAG^CTG
794 CAGCTG
SacI 205 GAGCTC GAGCT^C
1059 GAGCTC
StyI 573 CCATGG C^C[AT][AT]GG
735 CCATGG
#########################################
# 3 OR MORE CUT ENZYMES ( 62 enzymes) #
#########################################
AciI AluI AlwI AlwNI BanII
BbsI BbvI BmyI BpmI BpuAI
BsaJI BsiYI BslI BsmFI Bsp1286I
BsrI BssKI BstF5I BstNI BstYI
Cac8I CfoI Cfr13I DdeI DpnII
EcoRII FauI Fnu4HI FokI HaeII
HaeIII HapII HhaI HinP1I HinfI
HpaII HphI ItaI MboI MboII
MflI MnlI MslI MspI MvaI
MwoI NdeII NlaIII NlaIV PleI
PstI Sau3AI Sau96I ScrFI SfaNI
SfcI StuI TfiI TseI Tsp509I
TspRI XhoII