Restriction map for
pFN21AB5934
(Circular) (Six-base) MAPSORT of: pFN21AB5934.seq Check: 9002 from: 1 to: 6319
pFN21AB5934 6319 bp
With 182 enzymes: SgfI *
July 6, 2012 17:34 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4129 278
Size: 53 83 186 936 2593 2468
Fragments arranged by size:
2593 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2048 3662 1937
Size: 111 1614 4594
Fragments arranged by size:
4594 1614 111
Acc65I G'GTAC_C
Cuts at: 3640 3640
Size: 6319
AclI AA'CG_TT
Cuts at: 1563 4446 4819 1563
Size: 2883 373 3063
Fragments arranged by size:
3063 2883 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3124 3208 4382 5766 6034 1632
Size: 1492 84 1174 1384 268 1917
Fragments arranged by size:
1917 1492 1384 1174 268 84
AfeI AGC'GCT
Cuts at: 2147 2147
Size: 6319
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6122
AflIII A'CryG_T
Cuts at: 1203 2873 5218 6195 1203
Size: 1670 2345 977 1327
Fragments arranged by size:
2345 1670 1327 977
AgeI A'CCGG_T
Cuts at: 5150 5150
Size: 6319
AhdI GACnn_n'nnGTC
Cuts at: 5048 5048
Size: 6319
AleI CACnn'nnGTG
Cuts at: 1166 3015 3099 3513 1166
Size: 1849 84 414 3972
Fragments arranged by size:
3972 1849 414 84
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2807 2940 1184
Size: 1623 133 4563
Fragments arranged by size:
4563 1623 133
AlwNI CAG_nnn'CTG
Cuts at: 2227 2260 2287 2885 4062 5634 2227
Size: 33 27 598 1177 1572 2912
Fragments arranged by size:
2912 1572 1177 598 33 27
ApaLI G'TGCA_C
Cuts at: 4376 5532 4376
Size: 1156 5163
ApoI r'AATT_y
Cuts at: 1493 3561 3628 3854 4006 6115 1493
Size: 2068 67 226 152 2109 1697
Fragments arranged by size:
2109 2068 1697 226 152 67
AseI AT'TA_AT
Cuts at: 160 1032 4873 160
Size: 872 3841 1606
Fragments arranged by size:
3841 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6319
AvaI C'yCGr_G
Cuts at: 1870 1943 3644 1870
Size: 73 1701 4545
Fragments arranged by size:
4545 1701 73
BamHI G'GATC_C
Cuts at: 2531 3342 3649 4016 2531
Size: 811 307 367 4834
Fragments arranged by size:
4834 811 367 307
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2398 3640 5095 618
Size: 325 217 627 611 1242 1455 1842
Fragments arranged by size:
1842 1455 1242 627 611 325 217
BanII G_rGCy'C
Cuts at: 1391 2138 2214 3068 3638 1391
Size: 747 76 854 570 4072
Fragments arranged by size:
4072 854 747 570 76
BbeI G_GCGC'C
Cuts at: 2402 2402
Size: 6319
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2061 2859 3224 928
Size: 971 162 798 365 4023
Fragments arranged by size:
4023 971 798 365 162
BbvCI CC'TCA_GC
Cuts at: 2802 2802
Size: 6319
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 2196 2230 4510 4544 1903
Size: 34 259 34 2280 34 3678
Fragments arranged by size:
3678 2280 259 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4211 5421 4211
Size: 1210 5109
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6319
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4930 136
Size: 107 122 71 631 605 3258 1525
Fragments arranged by size:
3258 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 2119 6314 2119
Size: 4195 2124
BlpI GC'TnA_GC
Cuts at: 3736 3736
Size: 6319
Bme1580I G_kGCm'C
Cuts at: 1790 4380 5536 1790
Size: 2590 1156 2573
Fragments arranged by size:
2590 2573 1156
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 6319
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2038 2103 3192
Size: 416 53 83 399 24 621 65 1089
Cuts at: 3192 3276 3360 4050 5008 442
Size: 84 84 690 958 1753
Fragments arranged by size:
1753 1089 958 690 621 416 399 84
84 83 65 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6319
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2991 2991
Size: 6319
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2282 2297 2543 2610 2781 4979 1239
Size: 1043 15 246 67 171 2198 2579
Fragments arranged by size:
2579 2198 1043 246 171 67 15
Bpu10I CC'TnA_GC
Cuts at: 2802 2983 3375 2802
Size: 181 392 5746
Fragments arranged by size:
5746 392 181
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2498 4444 5309 5607 2498
Size: 1946 865 298 3210
Fragments arranged by size:
3210 1946 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2758 4982 882
Size: 617 1259 2224 2219
Fragments arranged by size:
2224 2219 1259 617
BsaAI yAC'GTr
Cuts at: 493 1204 4110 493
Size: 711 2906 2702
Fragments arranged by size:
2906 2702 711
BsaBI GATnn'nnATC
Cuts at: 6313 6313
Size: 6319
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2399 4126 4508 5143
Size: 53 83 186 936 866 1727 382 635
Cuts at: 5143 275
Size: 1451
Fragments arranged by size:
1727 1451 936 866 635 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 4752 5150 5424 5571 5982 4752
Size: 398 274 147 411 5089
Fragments arranged by size:
5089 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 3263 3293 1178
Size: 30 165 30 1860 30 4204
Fragments arranged by size:
4204 1860 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2363 2819 1186
Size: 1177 456 4686
Fragments arranged by size:
4686 1177 456
BseYI C'CCAG_C
Cuts at: 2316 2535 5522 6018 2316
Size: 219 2987 496 2617
Fragments arranged by size:
2987 2617 496 219
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2898 3307 1163
Size: 573 1162 409 4175
Fragments arranged by size:
4175 1162 573 409
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3769 4530 5558 1540
Size: 275 186 1768 761 1028 2301
Fragments arranged by size:
2301 1768 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2214 3068 4380 4465 5536 2214
Size: 854 1312 85 1071 2997
Fragments arranged by size:
2997 1312 1071 854 85
BsmI GAATG_Cn'
Cuts at: 3830 3923 3830
Size: 93 6226
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2138 2214 3068 3638 4380 4465 5536
Size: 399 348 76 854 570 742 85 1071
Cuts at: 5536 1391
Size: 2174
Fragments arranged by size:
2174 1071 854 742 570 399 348 85
76
BspEI T'CCGG_A
Cuts at: 5982 5982
Size: 6319
BspHI T'CATG_A
Cuts at: 4208 5938 4208
Size: 1730 4589
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2395 3674 844
Size: 1551 1279 3489
Fragments arranged by size:
3489 1551 1279
BsrBI CCG'CTC
Cuts at: 1394 1823 2343 4206 1394
Size: 429 520 1863 3507
Fragments arranged by size:
3507 1863 520 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2856 4814 4988 65
Size: 1183 1608 1958 174 1396
Fragments arranged by size:
1958 1608 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2099 2395 4963 5150 1700
Size: 110 289 296 2568 187 2869
Fragments arranged by size:
2869 2568 296 289 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6319
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6319
BssSI C'ACGA_G
Cuts at: 4379 5391 4379
Size: 1012 5307
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2434 794
Size: 1640 4679
BstBI TT'CG_AA
Cuts at: 5123 5123
Size: 6319
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6319
BstXI CCAn_nnnn'nTGG
Cuts at: 2960 2960
Size: 6319
BstYI r'GATC_y
Cuts at: 1972 2119 2256 2531 3342 3649 4016 4401 4418
Size: 147 137 275 811 307 367 385 17
Cuts at: 4418 5870 5956 5968 6314 1972
Size: 1452 86 12 346 1977
Fragments arranged by size:
1977 1452 811 385 367 346 307 275
147 137 86 17 12
BtgI C'CryG_G
Cuts at: 513 1065 2004 2578 2740 513
Size: 552 939 574 162 4092
Fragments arranged by size:
4092 939 574 552 162
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5580
Fragments arranged by size:
5580 683 56
BtsI GCAGTG_nn'
Cuts at: 2610 3835 4629 4649 2610
Size: 1225 794 20 4280
Fragments arranged by size:
4280 1225 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6319
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6284
DraI TTT'AAA
Cuts at: 3623 3623
Size: 6319
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5326 809
Size: 507 4010 1802
Fragments arranged by size:
4010 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 3766 4655 6284
Size: 54 1425 50 275 18 1936 889 1629
Cuts at: 6284 8
Size: 43
Fragments arranged by size:
1936 1629 1425 889 275 54 50 43
18
EagI C'GGCC_G
Cuts at: 1537 1812 3766 1537
Size: 275 1954 4090
Fragments arranged by size:
4090 1954 275
EarI CTCTTCn'nnn_
Cuts at: 1353 3033 4249 1353
Size: 1680 1216 3423
Fragments arranged by size:
3423 1680 1216
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4906 5280 5426 387
Size: 1064 138 3317 374 146 1280
Fragments arranged by size:
3317 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 2212 3066 2212
Size: 854 5465
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2282 2297 2543 2610 2781 3124 3208
Size: 393 650 15 246 67 171 343 84
Cuts at: 3208 4382 4979 5766 6034 1239
Size: 1174 597 787 268 1524
Fragments arranged by size:
1524 1174 787 650 597 393 343 268
246 171 84 67 15
EcoNI CCTnn'n_nnAGG
Cuts at: 3614 3614
Size: 6319
EcoO109I rG'GnC_Cy
Cuts at: 2180 2563 2180
Size: 383 5936
EcoRI G'AATT_C
Cuts at: 3628 4006 3628
Size: 378 5941
EcoRV GAT'ATC
Cuts at: 2835 2835
Size: 6319
FspI TGC'GCA
Cuts at: 4825 4825
Size: 6319
HaeII r_GCGC'y
Cuts at: 1678 2149 2195 2402 5466 1678
Size: 471 46 207 3064 2531
Fragments arranged by size:
3064 2531 471 207 46
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2030 2062 4960 4992 5034
Size: 32 135 32 458 32 2898 32 42
Cuts at: 5034 5066 1373
Size: 32 2626
Fragments arranged by size:
2898 2626 458 135 42 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2419 3663 3909 669
Size: 988 281 481 1244 246 3079
Fragments arranged by size:
3079 1244 988 481 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6319
HpaI GTT'AAC
Cuts at: 3909 3909
Size: 6319
KasI G'GCGC_C
Cuts at: 2398 2398
Size: 6319
KpnI G_GTAC'C
Cuts at: 3644 3644
Size: 6319
MfeI C'AATT_G
Cuts at: 3918 3918
Size: 6319
MluI A'CGCG_T
Cuts at: 6195 6195
Size: 6319
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 3396 5433 5617 1305
Size: 2091 2037 184 2007
Fragments arranged by size:
2091 2037 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 4497
Fragments arranged by size:
4497 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2081 2958 3015 3099 3115 3513 4277
Size: 648 915 877 57 84 16 398 764
Cuts at: 4277 4636 4795 518
Size: 359 159 2042
Fragments arranged by size:
2042 915 877 764 648 398 359 159
84 57 16
MspA1I CmG'CkG
Cuts at: 1606 2386 2801 3735 4412 5560 5805 1606
Size: 780 415 934 677 1148 245 2120
Fragments arranged by size:
2120 1148 934 780 677 415 245
NaeI GCC'GGC
Cuts at: 2397 2397
Size: 6319
NarI GG'CG_CC
Cuts at: 2399 2399
Size: 6319
NcoI C'CATG_G
Cuts at: 513 1065 2004 2578 2740 513
Size: 552 939 574 162 4092
Fragments arranged by size:
4092 939 574 552 162
NdeI CA'TA_TG
Cuts at: 387 3112 387
Size: 2725 3594
NgoMIV G'CCGG_C
Cuts at: 2395 2395
Size: 6319
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6319
NotI GC'GGCC_GC
Cuts at: 3766 3766
Size: 6319
NspI r_CATG'y
Cuts at: 1132 1229 2877 3677 5222 1132
Size: 97 1648 800 1545 2229
Fragments arranged by size:
2229 1648 1545 800 97
PasI CC'CwG_GG
Cuts at: 1348 2471 2635 2758 1348
Size: 1123 164 123 4909
Fragments arranged by size:
4909 1123 164 123
PciI A'CATG_T
Cuts at: 2873 5218 2873
Size: 2345 3974
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 6319
PfoI T'CCnGG_A
Cuts at: 2259 2259
Size: 6319
PmeI GTTT'AAAC
Cuts at: 3623 3623
Size: 6319
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4388 4420 5901 5933 4388
Size: 32 1481 32 4774
Fragments arranged by size:
4774 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2563 2563
Size: 6319
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6319
PsiI TTA'TAA
Cuts at: 3444 3889 3444
Size: 445 5874
PstI C_TGCA'G
Cuts at: 830 2179 2232 2313 2594 3239 3671 830
Size: 1349 53 81 281 645 432 3478
Fragments arranged by size:
3478 1349 645 432 281 81 53
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6319
PvuII CAG'CTG
Cuts at: 2386 2801 2386
Size: 415 5904
SacI G_AGCT'C
Cuts at: 2214 3068 2214
Size: 854 5465
SalI G'TCGA_C
Cuts at: 1936 3661 1936
Size: 1725 4594
SbfI CC_TGCA'GG
Cuts at: 3671 3671
Size: 6319
ScaI AGT'ACT
Cuts at: 4567 6279 4567
Size: 1712 4607
SfcI C'TryA_G
Cuts at: 826 1045 2049 2175 2228 2309 2590 2716 3235
Size: 219 1004 126 53 81 281 126 519
Cuts at: 3235 3667 4802 5483 5674 826
Size: 432 1135 681 191 1471
Fragments arranged by size:
1471 1135 1004 681 519 432 281 219
191 126 126 81 53
SfoI GGC'GCC
Cuts at: 2400 2400
Size: 6319
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6319
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6319
SmaI CCC'GGG
Cuts at: 1872 3646 1872
Size: 1774 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2513 4423 5324 5586 820
Size: 197 926 570 1910 901 262 1553
Fragments arranged by size:
1910 1553 926 901 570 262 197
SnaBI TAC'GTA
Cuts at: 493 4110 493
Size: 3617 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6319
SphI G_CATG'C
Cuts at: 1132 3677 1132
Size: 2545 3774
SspI AAT'ATT
Cuts at: 5 52 4243 5
Size: 47 4191 2081
Fragments arranged by size:
4191 2081 47
StuI AGG'CCT
Cuts at: 1517 2988 3137 3389 3486 1517
Size: 1471 149 252 97 4350
Fragments arranged by size:
4350 1471 252 149 97
StyI C'CwwG_G
Cuts at: 513 1065 2004 2578 2740 3758 513
Size: 552 939 574 162 1018 3074
Fragments arranged by size:
3074 1018 939 574 552 162
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2308 2750 4346 4363 4516 4701 5146
Size: 1255 728 442 1596 17 153 185 445
Cuts at: 5146 325
Size: 1498
Fragments arranged by size:
1596 1498 1255 728 445 442 185 153
17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 4565 6277 96
Size: 274 80 33 51 1443 2588 1712 138
Fragments arranged by size:
2588 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 6279 6311 6279
Size: 32 6287
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6319
XbaI T'CTAG_A
Cuts at: 3655 3655
Size: 6319
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6319
XmaI C'CCGG_G
Cuts at: 1870 3644 1870
Size: 1774 4545
XmnI GAAnn'nnTTC
Cuts at: 3221 4448 3221
Size: 1227 5092
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4127 276
Size: 53 83 186 936 2593 2468
Fragments arranged by size:
2593 2468 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AfeI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbeI BbsI BbvCI
BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI
BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI
BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI
BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI
EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I
EcoRI EcoRV FspI HaeII Hin4I HincII HindIII HpaI
KasI KpnI MfeI MluI MmeI MscI MslI MspA1I
NaeI NarI NcoI NdeI NgoMIV NheI NotI NspI
PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI
PsiI PstI PvuI PvuII SacI SalI SbfI ScaI
SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI
SphI SspI StuI StyI TaqII TatI TstI Tth111I
XbaI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AloI ApaI AscI AvrII BaeI BfrBI BsiWI
BsmBI BstZ17I Bsu36I DraIII FalI FseI FspAI NruI
NsiI PacI PmlI PspOMI PspXI PsrI RsrII SacII
SanDI SapI SexAI SfiI SrfI SwaI XcmI