(Linear) (Six-base) MAPSORT of: KIBB5934.seq Check: 187 from: 1 to: 1614 KIBB5934.orf With 182 enzymes: SgfI * July 3, 2012 16:12 .. AccI GT'mk_AC Cuts at: 0 43 1614 Size: 43 1571 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 0 1119 1203 1614 Size: 1119 84 411 Fragments arranged by size: 1119 411 84 AfeI AGC'GCT Cuts at: 0 142 1614 Size: 142 1472 AflIII A'CryG_T Cuts at: 0 868 1614 Size: 868 746 AleI CACnn'nnGTG Cuts at: 0 1010 1094 1508 1614 Size: 1010 84 414 106 Fragments arranged by size: 1010 414 106 84 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 0 802 935 1614 Size: 802 133 679 Fragments arranged by size: 802 679 133 AlwNI CAG_nnn'CTG Cuts at: 0 222 255 282 880 1614 Size: 222 33 27 598 734 Fragments arranged by size: 734 598 222 33 27 ApoI r'AATT_y Cuts at: 0 1556 1614 Size: 1556 58 BamHI G'GATC_C Cuts at: 0 526 1337 1614 Size: 526 811 277 Fragments arranged by size: 811 526 277 BanI G'GyrC_C Cuts at: 0 393 1614 Size: 393 1221 BanII G_rGCy'C Cuts at: 0 133 209 1063 1614 Size: 133 76 854 551 Fragments arranged by size: 854 551 133 76 BbeI G_GCGC'C Cuts at: 0 397 1614 Size: 397 1217 BbsI GAAGACnn'nnnn_ Cuts at: 0 56 854 1219 1614 Size: 56 798 365 395 Fragments arranged by size: 798 395 365 56 BbvCI CC'TCA_GC Cuts at: 0 797 1614 Size: 797 817 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 0 191 225 1614 Size: 191 34 1389 Fragments arranged by size: 1389 191 34 BglII A'GATC_T Cuts at: 0 114 1614 Size: 114 1500 BmrI ACTGGGnnnn_n' Cuts at: 0 33 98 1187 1271 1355 1614 Size: 33 65 1089 84 84 259 Fragments arranged by size: 1089 259 84 84 65 33 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 0 986 1614 Size: 986 628 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 277 292 538 605 776 1614 Size: 277 15 246 67 171 838 Fragments arranged by size: 838 277 246 171 67 15 Bpu10I CC'TnA_GC Cuts at: 0 797 978 1370 1614 Size: 797 181 392 244 Fragments arranged by size: 797 392 244 181 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 493 1614 Size: 493 1121 BsaI GGTCTCn'nnnn_ Cuts at: 0 753 1614 Size: 753 861 BsaHI Gr'CG_yC Cuts at: 0 394 1614 Size: 394 1220 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 0 1258 1288 1614 Size: 1258 30 326 Fragments arranged by size: 1258 326 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 0 358 814 1614 Size: 358 456 800 Fragments arranged by size: 800 456 358 BseYI C'CCAG_C Cuts at: 0 311 530 1614 Size: 311 219 1084 Fragments arranged by size: 1084 311 219 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 0 893 1302 1614 Size: 893 409 312 Fragments arranged by size: 893 409 312 BsiHKAI G_wGCw'C Cuts at: 0 209 1063 1614 Size: 209 854 551 Fragments arranged by size: 854 551 209 Bsp1286I G_dGCh'C Cuts at: 0 133 209 1063 1614 Size: 133 76 854 551 Fragments arranged by size: 854 551 133 76 BspMI ACCTGCnnnn'nnnn_ Cuts at: 0 390 1614 Size: 390 1224 BsrBI CCG'CTC Cuts at: 0 338 1614 Size: 338 1276 BsrDI GCAATG_nn' Cuts at: 0 851 1614 Size: 851 763 BsrFI r'CCGG_y Cuts at: 0 94 390 1614 Size: 94 296 1224 Fragments arranged by size: 1224 296 94 BstAPI GCAn_nnn'nTGC Cuts at: 0 429 1614 Size: 429 1185 BstXI CCAn_nnnn'nTGG Cuts at: 0 955 1614 Size: 955 659 BstYI r'GATC_y Cuts at: 0 114 251 526 1337 1614 Size: 114 137 275 811 277 Fragments arranged by size: 811 277 275 137 114 BtgI C'CryG_G Cuts at: 0 573 735 1614 Size: 573 162 879 Fragments arranged by size: 879 573 162 BtsI GCAGTG_nn' Cuts at: 0 605 1614 Size: 605 1009 EarI CTCTTCn'nnn_ Cuts at: 0 1028 1614 Size: 1028 586 EcoICRI GAG'CTC Cuts at: 0 207 1061 1614 Size: 207 854 553 Fragments arranged by size: 854 553 207 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 0 277 292 538 605 776 1119 1203 1614 Size: 277 15 246 67 171 343 84 411 Fragments arranged by size: 411 343 277 246 171 84 67 15 EcoO109I rG'GnC_Cy Cuts at: 0 175 558 1614 Size: 175 383 1056 Fragments arranged by size: 1056 383 175 EcoRV GAT'ATC Cuts at: 0 830 1614 Size: 830 784 HaeII r_GCGC'y Cuts at: 0 144 190 397 1614 Size: 144 46 207 1217 Fragments arranged by size: 1217 207 144 46 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 0 25 57 1614 Size: 25 32 1557 Fragments arranged by size: 1557 32 25 HincII GTy'rAC Cuts at: 0 414 1614 Size: 414 1200 KasI G'GCGC_C Cuts at: 0 393 1614 Size: 393 1221 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 0 1391 1614 Size: 1391 223 MslI CAynn'nnrTG Cuts at: 0 76 953 1010 1094 1110 1508 1614 Size: 76 877 57 84 16 398 106 Fragments arranged by size: 877 398 106 84 76 57 16 MspA1I CmG'CkG Cuts at: 0 381 796 1614 Size: 381 415 818 Fragments arranged by size: 818 415 381 NaeI GCC'GGC Cuts at: 0 392 1614 Size: 392 1222 NarI GG'CG_CC Cuts at: 0 394 1614 Size: 394 1220 NcoI C'CATG_G Cuts at: 0 573 735 1614 Size: 573 162 879 Fragments arranged by size: 879 573 162 NdeI CA'TA_TG Cuts at: 0 1107 1614 Size: 1107 507 NgoMIV G'CCGG_C Cuts at: 0 390 1614 Size: 390 1224 NspI r_CATG'y Cuts at: 0 872 1614 Size: 872 742 PasI CC'CwG_GG Cuts at: 0 466 630 753 1614 Size: 466 164 123 861 Fragments arranged by size: 861 466 164 123 PciI A'CATG_T Cuts at: 0 868 1614 Size: 868 746 PfoI T'CCnGG_A Cuts at: 0 254 1614 Size: 254 1360 PpuMI rG'GwC_Cy Cuts at: 0 558 1614 Size: 558 1056 PsiI TTA'TAA Cuts at: 0 1439 1614 Size: 1439 175 PstI C_TGCA'G Cuts at: 0 174 227 308 589 1234 1614 Size: 174 53 81 281 645 380 Fragments arranged by size: 645 380 281 174 81 53 PvuII CAG'CTG Cuts at: 0 381 796 1614 Size: 381 415 818 Fragments arranged by size: 818 415 381 SacI G_AGCT'C Cuts at: 0 209 1063 1614 Size: 209 854 551 Fragments arranged by size: 854 551 209 SfcI C'TryA_G Cuts at: 0 44 170 223 304 585 711 1230 1614 Size: 44 126 53 81 281 126 519 384 Fragments arranged by size: 519 384 281 126 126 81 53 44 SfoI GGC'GCC Cuts at: 0 395 1614 Size: 395 1219 SmlI C'TyrA_G Cuts at: 0 508 1614 Size: 508 1106 StuI AGG'CCT Cuts at: 0 983 1132 1384 1481 1614 Size: 983 149 252 97 133 Fragments arranged by size: 983 252 149 133 97 StyI C'CwwG_G Cuts at: 0 573 735 1614 Size: 573 162 879 Fragments arranged by size: 879 573 162 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 0 303 745 1614 Size: 303 442 869 Fragments arranged by size: 869 442 303 XmnI GAAnn'nnTTC Cuts at: 0 1216 1614 Size: 1216 398 Enzymes that do cut: AccI AcuI AfeI AflIII AleI AlfI AlwNI ApoI BamHI BanI BanII BbeI BbsI BbvCI BcgI BglII BmrI BplI BpmI Bpu10I BpuEI BsaI BsaHI BsaXI BseRI BseYI BsgI BsiHKAI Bsp1286I BspMI BsrBI BsrDI BsrFI BstAPI BstXI BstYI BtgI BtsI EarI EcoICRI Eco57MI EcoO109I EcoRV HaeII Hin4I HincII KasI MmeI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV NspI PasI PciI PfoI PpuMI PsiI PstI PvuII SacI SfcI SfoI SmlI StuI StyI TaqII XmnI Enzymes that do not cut: AarI AatII Acc65I AclI AflII AgeI AhdI AloI ApaI ApaLI AscI AseI AsiSI AvaI AvrII BaeI BciVI BclI BfrBI BglI BlpI Bme1580I BmgBI BmtI BsaAI BsaBI BsaWI BsiEI BsiWI BsmI BsmBI BspEI BspHI BsrGI BssHII BssSI BstBI BstEII BstZ17I Bsu36I BtgZI ClaI CspCI DraI DraIII DrdI EaeI EagI EciI EcoNI EcoRI FalI FseI FspI FspAI HindIII HpaI KpnI MfeI MluI MscI NheI NotI NruI NsiI PacI PflMI PmeI PmlI PpiI PshAI PspOMI PspXI PsrI PvuI RsrII SacII SalI SanDI SapI SbfI ScaI SexAI SfiI SgfI SgrAI SmaI SnaBI SpeI SphI SrfI SspI SwaI TatI TstI Tth111I XbaI XcmI XhoI XmaI ZraI