################################## # NO CUT ENZYMES ( 118 enzymes) # ################################## AatII Acc65I AccIII AclI AcsI AflII AflIII AgeI AhdI AlwI Aor51HI ApoI AscI AseI AsnI Asp700I Asp718I AspEI AspI AviII AvrII BalI BamHI BbsI BbvCI BciVI BclI BfaI BfrI BlnI BpuAI BsaBI BseAI BsiWI BsmBI BsmI Bsp1407I BspDI BspEI BspHI BspLU11I BsrDI BsrFI BsrGI Bst1107I BstAPI BstBI BstEII BstPI BstZ17I Cfr10I ClaI CpoI DraI DrdI EagI Eam1105I EclXI Eco47III Eco52I EcoO65I EcoRI EcoRV EcoT22I FbaI FseI FspI HincII HindII HindIII HpaI HphI KpnI MaeI MamI MfeI MluI MluNI MroI MscI MseI MslI MunI NaeI NdeI NgoMIV NheI NotI NruI NsiI NspI NspV PacI PaeR7I PflFI PinAI PmeI PshAI PshBI Psp1406I PvuI RcaI RsrII SalI SapI SfuI SgrAI SnaBI SpeI SphI SspBI SspI SwaI Tru9I Tth111I XbaI XhoI XmnI ################################### # ONE CUT ENZYMES ( 39 enzymes) # ################################### AccI 616 GTCTAC GT^[AC][GT]AC BbeI 1156 GGCGCC GGCGC^C BbrPI 706 CACGTG CAC^GTG BcgI 904 CGAGGGGCCTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10) BglII 605 AGATCT A^GATCT BsaAI 706 CACGTG [CT]AC^GT[GA] BsaWI 513 ACCGGA [AT]^CCGG[AT] BssHII 291 GCGCGC G^CGCGC BssSI 436 CACGAG CACGAG(-5/-1) BstXI 56 CCAGCTCTTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG BstYI 605 AGATCT [GA]^GATC[CT] DraIII 997 CACAGCGTG CAC[ACGT][ACGT][ACGT]^GTG EaeI 1087 CGGCCA [CT]^GGCC[GA] EarI 775 CTCTTC CTCTTC(1/4) EcoT14I 1248 CCATGG C^C[AT][AT]GG KasI 1156 GGCGCC G^GCGCC Ksp632I 775 CTCTTC CTCTTC(1/4) MaeII 707 ACGT A^CGT MaeIII 987 GTCAC ^GT[ACGT]AC MflI 605 AGATCT [GA]^GATC[CT] NarI 1156 GGCGCC GG^CGCC NcoI 1248 CCATGG C^CATGG PmaCI 706 CACGTG CAC^GTG PmlI 706 CACGTG CAC^GTG SacI 79 GAGCTC GAGCT^C SbfI 480 CCTGCAGG CCTGCA^GG ScaI 846 AGTACT AGT^ACT SexAI 23 ACCTGGT A^CC[AT]GGT SfoI 1156 GGCGCC GGC^GCC SmaI 1268 CCCGGG CCC^GGG SmlI 490 CTCAAG C^T[CT][GA]AG Sse8387I 480 CCTGCAGG CCTGCA^GG StyI 1248 CCATGG C^C[AT][AT]GG Tsp45I 987 GTCAC ^GT[GC]AC Tsp509I 425 AATT ^AATT XcmI 1313 CCACCTCCTTCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG XhoII 605 AGATCT [GA]^GATC[CT] XmaCI 1268 CCCGGG C^CCGGG XmaI 1268 CCCGGG C^CCGGG ################################### # TWO CUT ENZYMES ( 24 enzymes) # ################################### Alw44I 709 GTGCAC G^TGCAC 994 GTGCAC AocI 454 CCTCAGG CC^T[ACGT]AGG 1165 CCTGAGG ApaLI 709 GTGCAC G^TGCAC 994 GTGCAC BlpI 81 GCTCAGC GC^T[ACGT]AGC 137 GCTCAGC Bpu1102I 81 GCTCAGC GC^T[ACGT]AGC 137 GCTCAGC BsaI 380 GAGACC GGTCTC(1/5) 397 GAGACC BsiEI 111 CGACCG CG[GA][CT]^CG 511 CGACCG BsrBI 783 CCGCTC CCGCTC(-3/-3) 1015 GAGCGG Bsu36I 454 CCTCAGG CC^T[ACGT]AGG 1165 CCTGAGG CelII 81 GCTCAGC GC^T[ACGT]AGC 137 GCTCAGC DpnII 606 GATC ^GATC 1035 GATC Eco81I 454 CCTCAGG CC^T[ACGT]AGG 1165 CCTGAGG HaeII 882 GGCGCT [GA]GCGC^[CT] 1156 GGCGCC HgaI 664 GACGC GACGC(5/10) 734 GCGTC KspI 1213 CCGCGG CCGC^GG 1233 CCGCGG MboI 606 GATC ^GATC 1035 GATC MboII 336 GAAGA GAAGA(8/7) 776 TCTTC NdeII 606 GATC ^GATC 1035 GATC PflMI 6 CCAGGCCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 1131 CCAGCAGCTGG SacII 1213 CCGCGG CCGC^GG 1233 CCGCGG Sau3AI 606 GATC ^GATC 1035 GATC SfiI 1041 GGCCCAGCTGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC 1289 GGCCCCCCAGGCC TfiI 122 GATTC G^A[AT]TC 1063 GATTC Van91I 6 CCAGGCCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 1131 CCAGCAGCTGG ######################################### # 3 OR MORE CUT ENZYMES ( 77 enzymes) # ######################################### AccII AciI AcyI AfaI AluI AlwNI ApaI AspHI AvaI AvaII BanI BanII BbvI BcnI BglI BmyI BpmI BsaHI BsaJI BseRI BsgI BsiHKAI BsiYI BslI BsmAI BsmFI BsoBI Bsp1286I BspMI BsrI BssKI BstF5I BstNI BstUI Cac8I CfoI Cfr13I DdeI DraII DsaI EcoNI EcoO109I EcoRII FauI Fnu4HI FokI HaeIII HapII HhaI Hin1I HinP1I HinfI HpaII ItaI MnlI MspA1I MspI MvaI MvnI MwoI NciI NlaIII NlaIV PleI PpuMI PstI PvuII RsaI Sau96I ScrFI SfaNI SfcI StuI TaqI TseI TspRI TthHB8I