(Circular) (Six-base) MAPSORT of: pFN21AE4085.seq Check: 5321 from: 1 to: 6043 pFN21AE4085 6043 bp With 182 enzymes: SgfI * July 6, 2012 17:34 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2179 2479 2583 2722 2733 2179 Size: 300 104 139 11 5489 Fragments arranged by size: 5489 300 139 104 11 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3853 278 Size: 53 83 186 936 2317 2468 Fragments arranged by size: 2468 2317 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2622 3386 1937 Size: 685 764 4594 Fragments arranged by size: 4594 764 685 Acc65I G'GTAC_C Cuts at: 3364 3364 Size: 6043 AclI AA'CG_TT Cuts at: 1563 4170 4543 1563 Size: 2607 373 3063 Fragments arranged by size: 3063 2607 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 4106 5490 5758 1632 Size: 2474 1384 268 1917 Fragments arranged by size: 2474 1917 1384 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5846 AflIII A'CryG_T Cuts at: 1203 4942 5919 1203 Size: 3739 977 1327 Fragments arranged by size: 3739 1327 977 AgeI A'CCGG_T Cuts at: 4874 4874 Size: 6043 AhdI GACnn_n'nnGTC Cuts at: 4772 4772 Size: 6043 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 6043 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 2435 1184 Size: 1251 4792 AlwNI CAG_nnn'CTG Cuts at: 2017 2368 3142 3786 5358 2017 Size: 351 774 644 1572 2702 Fragments arranged by size: 2702 1572 774 644 351 ApaI G_GGCC'C Cuts at: 2329 2550 2646 3118 3274 3281 3297 2329 Size: 221 96 472 156 7 16 5075 Fragments arranged by size: 5075 472 221 156 96 16 7 ApaLI G'TGCA_C Cuts at: 2714 2999 4100 5256 2714 Size: 285 1101 1156 3501 Fragments arranged by size: 3501 1156 1101 285 ApoI r'AATT_y Cuts at: 1493 3352 3578 3730 5839 1493 Size: 1859 226 152 2109 1697 Fragments arranged by size: 2109 1859 1697 226 152 AseI AT'TA_AT Cuts at: 160 1032 4597 160 Size: 872 3565 1606 Fragments arranged by size: 3565 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6043 AvaI C'yCGr_G Cuts at: 1870 1943 2042 2762 3267 3273 3368 1870 Size: 73 99 720 505 6 95 4545 Fragments arranged by size: 4545 720 505 99 95 73 6 BamHI G'GATC_C Cuts at: 3373 3740 3373 Size: 367 5676 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2354 2598 2843 3161 3175 Size: 325 217 627 567 244 245 318 14 Cuts at: 3175 3364 4819 618 Size: 189 1455 1842 Fragments arranged by size: 1842 1455 627 567 325 318 245 244 217 189 14 BanII G_rGCy'C Cuts at: 1391 2088 2329 2484 2550 2646 2947 3118 3210 Size: 697 241 155 66 96 301 171 92 Cuts at: 3210 3261 3274 3281 3297 3362 1391 Size: 51 13 7 16 65 4072 Fragments arranged by size: 4072 697 301 241 171 155 96 92 66 65 51 16 13 7 BbeI G_GCGC'C Cuts at: 3165 3165 Size: 6043 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 5072 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 2898 2932 4234 4268 1903 Size: 34 961 34 1302 34 3678 Fragments arranged by size: 3678 1302 961 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3935 5145 3935 Size: 1210 4833 BclI T'GATC_A Cuts at: 1548 1548 Size: 6043 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2277 3053 3301 Size: 107 122 71 631 605 605 776 248 Cuts at: 3301 4654 136 Size: 1353 1525 Fragments arranged by size: 1525 1353 776 631 605 605 248 122 107 71 BglII A'GATC_T Cuts at: 2610 6038 2610 Size: 3428 2615 BlpI GC'TnA_GC Cuts at: 2087 2143 3460 2087 Size: 56 1317 4670 Fragments arranged by size: 4670 1317 56 Bme1580I G_kGCm'C Cuts at: 1790 2329 2550 2646 2718 2897 3003 3118 3274 Size: 539 221 96 72 179 106 115 156 Cuts at: 3274 3281 3297 4104 5260 1790 Size: 7 16 807 1156 2573 Fragments arranged by size: 2573 1156 807 539 221 179 156 115 106 96 72 16 7 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 6043 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2233 2948 3774 Size: 416 53 83 399 24 816 715 826 Cuts at: 3774 4732 442 Size: 958 1753 Fragments arranged by size: 1753 958 826 816 715 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6043 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2233 2233 Size: 6043 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2044 2403 2951 4703 1239 Size: 805 359 548 1752 2579 Fragments arranged by size: 2579 1752 805 548 359 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2480 4168 5033 5331 2480 Size: 1688 865 298 3192 Fragments arranged by size: 3192 1688 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2379 2396 4706 882 Size: 617 880 17 2310 2219 Fragments arranged by size: 2310 2219 880 617 17 BsaAI yAC'GTr Cuts at: 493 1204 2713 3834 493 Size: 711 1509 1121 2702 Fragments arranged by size: 2702 1509 1121 711 BsaBI GATnn'nnATC Cuts at: 6037 6037 Size: 6043 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2670 2739 3162 3850 Size: 53 83 186 936 1137 69 423 688 Cuts at: 3850 4232 4867 275 Size: 382 635 1451 Fragments arranged by size: 1451 1137 936 688 635 423 382 186 83 69 53 BsaWI w'CCGG_w Cuts at: 2518 4476 4874 5148 5295 5706 2518 Size: 1958 398 274 147 411 2855 Fragments arranged by size: 2855 1958 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2186 2216 1178 Size: 30 165 30 783 30 5005 Fragments arranged by size: 5005 783 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2600 2993 3218 1186 Size: 1414 393 225 4011 Fragments arranged by size: 4011 1414 393 225 BseYI C'CCAG_C Cuts at: 2890 3048 3057 3135 5246 5742 2890 Size: 158 9 78 2111 496 3191 Fragments arranged by size: 3191 2111 496 158 78 9 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2054 2405 2423 2627 3212 1163 Size: 573 318 351 18 204 585 3994 Fragments arranged by size: 3994 585 573 351 318 204 18 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2119 2519 3493 4254 5282 1540 Size: 275 186 118 400 974 761 1028 2301 Fragments arranged by size: 2301 1028 974 761 400 275 186 118 BsiHKAI G_wGCw'C Cuts at: 2088 2496 2718 3003 4104 4189 5260 2088 Size: 408 222 285 1101 85 1071 2871 Fragments arranged by size: 2871 1101 1071 408 285 222 85 BsmI GAATG_Cn' Cuts at: 3554 3647 3554 Size: 93 5950 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2088 2329 2484 2496 2550 2646 2718 Size: 399 298 241 155 12 54 96 72 Cuts at: 2718 2897 2947 3003 3118 3210 3261 3274 3281 Size: 179 50 56 115 92 51 13 7 Cuts at: 3281 3297 3362 4104 4189 5260 1391 Size: 16 65 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 399 298 241 179 155 115 96 92 85 72 65 56 54 51 50 16 13 12 7 BspEI T'CCGG_A Cuts at: 5706 5706 Size: 6043 BspHI T'CATG_A Cuts at: 3932 5662 3932 Size: 1730 4313 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2179 2427 2479 2583 2722 2733 2766 3036 Size: 1335 248 52 104 139 11 33 270 Cuts at: 3036 3398 844 Size: 362 3489 Fragments arranged by size: 3489 1335 362 270 248 139 104 52 33 11 BsrBI CCG'CTC Cuts at: 1394 1823 2790 3022 3930 1394 Size: 429 967 232 908 3507 Fragments arranged by size: 3507 967 908 429 232 BsrDI GCAATG_nn' Cuts at: 65 1248 4538 4712 65 Size: 1183 3290 174 1396 Fragments arranged by size: 3290 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 4687 4874 1700 Size: 110 2877 187 2869 Fragments arranged by size: 2877 2869 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 6043 BssHII G'CGCG_C Cuts at: 1925 2296 1925 Size: 371 5672 BssSI C'ACGA_G Cuts at: 2441 4103 5115 2441 Size: 1662 1012 3369 Fragments arranged by size: 3369 1662 1012 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 6043 BstBI TT'CG_AA Cuts at: 4847 4847 Size: 6043 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 6043 BstXI CCAn_nnnn'nTGG Cuts at: 2068 2068 Size: 6043 BstYI r'GATC_y Cuts at: 1972 2610 3373 3740 4125 4142 5594 5680 5692 Size: 638 763 367 385 17 1452 86 12 Cuts at: 5692 6038 1972 Size: 346 1977 Fragments arranged by size: 1977 1452 763 638 385 367 346 86 17 12 Bsu36I CC'TnA_GG Cuts at: 2460 3171 2460 Size: 711 5332 BtgI C'CryG_G Cuts at: 513 1065 2004 2467 2550 3218 3238 3253 513 Size: 552 939 463 83 668 20 15 3303 Fragments arranged by size: 3303 939 668 552 463 83 20 15 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5304 Fragments arranged by size: 5304 683 56 BtsI GCAGTG_nn' Cuts at: 2273 2895 3559 4353 4373 2273 Size: 622 664 794 20 3943 Fragments arranged by size: 3943 794 664 622 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6043 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6008 DraI TTT'AAA Cuts at: 3347 3347 Size: 6043 DraIII CAC_nnn'GTG Cuts at: 3007 3007 Size: 6043 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5050 809 Size: 507 3734 1802 Fragments arranged by size: 3734 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 3092 3490 4379 Size: 54 1425 50 275 18 1262 398 889 Cuts at: 4379 6008 8 Size: 1629 43 Fragments arranged by size: 1629 1425 1262 889 398 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3490 1537 Size: 275 1678 4090 Fragments arranged by size: 4090 1678 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2786 3973 1353 Size: 1433 1187 3423 Fragments arranged by size: 3423 1433 1187 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2174 3092 3238 4630 5004 5150 Size: 1064 138 585 918 146 1392 374 146 Cuts at: 5150 387 Size: 1280 Fragments arranged by size: 1392 1280 1064 918 585 374 146 146 138 EcoICRI GAG'CTC Cuts at: 2086 2086 Size: 6043 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2044 2403 2951 4106 4703 5490 5758 Size: 393 412 359 548 1155 597 787 268 Cuts at: 5758 1239 Size: 1524 Fragments arranged by size: 1524 1155 787 597 548 412 393 359 268 EcoNI CCTnn'n_nnAGG Cuts at: 2021 2729 2837 2021 Size: 708 108 5227 Fragments arranged by size: 5227 708 108 EcoO109I rG'GnC_Cy Cuts at: 2014 2026 2325 2377 2416 2546 2643 2913 3025 Size: 12 299 52 39 130 97 270 112 Cuts at: 3025 3080 3114 3277 3278 3293 3294 3303 2014 Size: 55 34 163 1 15 1 9 4754 Fragments arranged by size: 4754 299 270 163 130 112 97 55 52 39 34 15 12 9 1 1 EcoRI G'AATT_C Cuts at: 3352 3730 3352 Size: 378 5665 FspI TGC'GCA Cuts at: 4549 4549 Size: 6043 HaeII r_GCGC'y Cuts at: 1678 2891 3165 5190 1678 Size: 1213 274 2025 2531 Fragments arranged by size: 2531 2025 1213 274 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2048 2080 4684 4716 4758 Size: 32 135 32 476 32 2604 32 42 Cuts at: 4758 4790 1373 Size: 32 2626 Fragments arranged by size: 2626 2604 476 135 42 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3387 3633 669 Size: 988 281 1449 246 3079 Fragments arranged by size: 3079 1449 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 6043 HpaI GTT'AAC Cuts at: 3633 3633 Size: 6043 KasI G'GCGC_C Cuts at: 3161 3161 Size: 6043 KpnI G_GTAC'C Cuts at: 3368 3368 Size: 6043 MfeI C'AATT_G Cuts at: 3642 3642 Size: 6043 MluI A'CGCG_T Cuts at: 5919 5919 Size: 6043 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2100 2722 2997 5157 5341 1305 Size: 795 622 275 2160 184 2007 Fragments arranged by size: 2160 2007 795 622 275 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 4221 Fragments arranged by size: 4221 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 4001 4360 4519 518 Size: 648 2835 359 159 2042 Fragments arranged by size: 2835 2042 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2091 2971 3034 3052 3061 3142 3220 3240 Size: 485 880 63 18 9 81 78 20 Cuts at: 3240 3459 4136 5284 5529 1606 Size: 219 677 1148 245 2120 Fragments arranged by size: 2120 1148 880 677 485 245 219 81 78 63 20 18 9 NarI GG'CG_CC Cuts at: 3162 3162 Size: 6043 NcoI C'CATG_G Cuts at: 513 1065 2004 3253 513 Size: 552 939 1249 3303 Fragments arranged by size: 3303 1249 939 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 6043 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6043 NotI GC'GGCC_GC Cuts at: 3490 3490 Size: 6043 NspI r_CATG'y Cuts at: 1132 1229 3401 4946 1132 Size: 97 2172 1545 2229 Fragments arranged by size: 2229 2172 1545 97 PasI CC'CwG_GG Cuts at: 1348 2017 2542 2630 1348 Size: 669 525 88 4761 Fragments arranged by size: 4761 669 525 88 PciI A'CATG_T Cuts at: 4942 4942 Size: 6043 PflMI CCAn_nnn'nTGG Cuts at: 1108 2017 3142 1108 Size: 909 1125 4009 Fragments arranged by size: 4009 1125 909 PfoI T'CCnGG_A Cuts at: 2928 3327 2928 Size: 399 5644 PmeI GTTT'AAAC Cuts at: 3347 3347 Size: 6043 PmlI CAC'GTG Cuts at: 2713 2713 Size: 6043 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4112 4144 5625 5657 4112 Size: 32 1481 32 4498 Fragments arranged by size: 4498 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2026 2416 3025 3080 2026 Size: 390 609 55 4989 Fragments arranged by size: 4989 609 390 55 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6043 PsiI TTA'TAA Cuts at: 3613 3613 Size: 6043 PspOMI G'GGCC_C Cuts at: 2325 2546 2642 3114 3270 3277 3293 2325 Size: 221 96 472 156 7 16 5075 Fragments arranged by size: 5075 472 221 156 96 16 7 PstI C_TGCA'G Cuts at: 830 2052 2176 2490 2730 2902 2976 3033 3395 Size: 1222 124 314 240 172 74 57 362 Cuts at: 3395 830 Size: 3478 Fragments arranged by size: 3478 1222 362 314 240 172 124 74 57 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6043 PvuII CAG'CTG Cuts at: 2971 3052 3061 3142 2971 Size: 81 9 81 5872 Fragments arranged by size: 5872 81 81 9 SacI G_AGCT'C Cuts at: 2088 2088 Size: 6043 SacII CC_GC'GG Cuts at: 3221 3241 3221 Size: 20 6023 SalI G'TCGA_C Cuts at: 1936 3385 1936 Size: 1449 4594 SanDI GG'GwC_CC Cuts at: 3080 3080 Size: 6043 SbfI CC_TGCA'GG Cuts at: 2490 3395 2490 Size: 905 5138 ScaI AGT'ACT Cuts at: 2853 4291 6003 2853 Size: 1438 1712 2893 Fragments arranged by size: 2893 1712 1438 SexAI A'CCwGG_T Cuts at: 2028 2028 Size: 6043 SfcI C'TryA_G Cuts at: 826 1045 2048 2172 2486 2726 2898 2972 3029 Size: 219 1003 124 314 240 172 74 57 Cuts at: 3029 3391 4526 5207 5398 826 Size: 362 1135 681 191 1471 Fragments arranged by size: 1471 1135 1003 681 362 314 240 219 191 172 124 74 57 SfiI GGCCn_nnn'nGGCC Cuts at: 3053 3301 3053 Size: 248 5795 SfoI GGC'GCC Cuts at: 3163 3163 Size: 6043 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6043 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6043 SmaI CCC'GGG Cuts at: 1872 3275 3370 1872 Size: 1403 95 4545 Fragments arranged by size: 4545 1403 95 SmlI C'TyrA_G Cuts at: 820 1017 1943 2495 4147 5048 5310 820 Size: 197 926 552 1652 901 262 1553 Fragments arranged by size: 1652 1553 926 901 552 262 197 SnaBI TAC'GTA Cuts at: 493 3834 493 Size: 3341 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6043 SphI G_CATG'C Cuts at: 1132 3401 1132 Size: 2269 3774 SspI AAT'ATT Cuts at: 5 52 3967 5 Size: 47 3915 2081 Fragments arranged by size: 3915 2081 47 StuI AGG'CCT Cuts at: 1517 2501 2591 2984 1517 Size: 984 90 393 4576 Fragments arranged by size: 4576 984 393 90 StyI C'CwwG_G Cuts at: 513 1065 2004 3253 3482 513 Size: 552 939 1249 229 3074 Fragments arranged by size: 3074 1249 939 552 229 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2181 2194 4070 4087 4240 4425 4870 Size: 1255 601 13 1876 17 153 185 445 Cuts at: 4870 325 Size: 1498 Fragments arranged by size: 1876 1498 1255 601 445 185 153 17 13 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2851 4289 6001 Size: 274 80 33 51 1443 874 1438 1712 Cuts at: 6001 96 Size: 138 Fragments arranged by size: 1712 1443 1438 874 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2186 2218 6003 6035 2186 Size: 32 3785 32 2194 Fragments arranged by size: 3785 2194 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 6043 XbaI T'CTAG_A Cuts at: 3379 3379 Size: 6043 XcmI CCAnnnn_n'nnnnTGG Cuts at: 3325 3325 Size: 6043 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6043 XmaI C'CCGG_G Cuts at: 1870 3273 3368 1870 Size: 1403 95 4545 Fragments arranged by size: 4545 1403 95 XmnI GAAnn'nnTTC Cuts at: 4172 4172 Size: 6043 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3851 276 Size: 53 83 186 936 2317 2468 Fragments arranged by size: 2468 2317 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NarI NcoI NdeI NheI NotI NspI PasI PciI PflMI PfoI PmeI PmlI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI PvuII SacI SacII SalI SanDI SbfI ScaI SexAI SfcI SfiI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI AloI AscI AvrII BaeI BbvCI BfrBI Bpu10I BsiWI BsmBI BstZ17I EcoRV FalI FseI FspAI NaeI NgoMIV NruI NsiI PacI PspXI PsrI RsrII SapI SrfI SwaI