(Linear) (Six-base) MAPSORT of: KIEE4085.seq Check: 155 from: 1 to: 1338 KIEE4085.orf With 182 enzymes: SgfI * July 3, 2012 18:30 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 0 174 474 578 717 728 1338 Size: 174 300 104 139 11 610 Fragments arranged by size: 610 300 174 139 104 11 AccI GT'mk_AC Cuts at: 0 617 1338 Size: 617 721 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 0 430 1338 Size: 430 908 AlwNI CAG_nnn'CTG Cuts at: 0 12 363 1137 1338 Size: 12 351 774 201 Fragments arranged by size: 774 351 201 12 ApaI G_GGCC'C Cuts at: 0 324 545 641 1113 1269 1276 1292 1338 Size: 324 221 96 472 156 7 16 46 Fragments arranged by size: 472 324 221 156 96 46 16 7 ApaLI G'TGCA_C Cuts at: 0 709 994 1338 Size: 709 285 344 Fragments arranged by size: 709 344 285 AvaI C'yCGr_G Cuts at: 0 37 757 1262 1268 1338 Size: 37 720 505 6 70 Fragments arranged by size: 720 505 70 37 6 BanI G'GyrC_C Cuts at: 0 349 593 838 1156 1170 1338 Size: 349 244 245 318 14 168 Fragments arranged by size: 349 318 245 244 168 14 BanII G_rGCy'C Cuts at: 0 83 324 479 545 641 942 1113 1205 Size: 83 241 155 66 96 301 171 92 Cuts at: 1205 1256 1269 1276 1292 1338 Size: 51 13 7 16 46 Fragments arranged by size: 301 241 171 155 96 92 83 66 51 46 16 13 7 BbeI G_GCGC'C Cuts at: 0 1160 1338 Size: 1160 178 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 0 893 927 1338 Size: 893 34 411 Fragments arranged by size: 893 411 34 BglI GCCn_nnn'nGGC Cuts at: 0 272 1048 1296 1338 Size: 272 776 248 42 Fragments arranged by size: 776 272 248 42 BglII A'GATC_T Cuts at: 0 605 1338 Size: 605 733 BlpI GC'TnA_GC Cuts at: 0 82 138 1338 Size: 82 56 1200 Fragments arranged by size: 1200 82 56 Bme1580I G_kGCm'C Cuts at: 0 324 545 641 713 892 998 1113 1269 Size: 324 221 96 72 179 106 115 156 Cuts at: 1269 1276 1292 1338 Size: 7 16 46 Fragments arranged by size: 324 221 179 156 115 106 96 72 46 16 7 BmrI ACTGGGnnnn_n' Cuts at: 0 228 943 1338 Size: 228 715 395 Fragments arranged by size: 715 395 228 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 0 228 1338 Size: 228 1110 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 39 398 946 1338 Size: 39 359 548 392 Fragments arranged by size: 548 392 359 39 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 475 1338 Size: 475 863 BsaI GGTCTCn'nnnn_ Cuts at: 0 374 391 1338 Size: 374 17 947 Fragments arranged by size: 947 374 17 BsaAI yAC'GTr Cuts at: 0 708 1338 Size: 708 630 BsaHI Gr'CG_yC Cuts at: 0 665 734 1157 1338 Size: 665 69 423 181 Fragments arranged by size: 665 423 181 69 BsaWI w'CCGG_w Cuts at: 0 513 1338 Size: 513 825 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 0 181 211 1338 Size: 181 30 1127 Fragments arranged by size: 1127 181 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 0 595 988 1213 1338 Size: 595 393 225 125 Fragments arranged by size: 595 393 225 125 BseYI C'CCAG_C Cuts at: 0 885 1043 1052 1130 1338 Size: 885 158 9 78 208 Fragments arranged by size: 885 208 158 78 9 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 0 49 400 418 622 1207 1338 Size: 49 351 18 204 585 131 Fragments arranged by size: 585 351 204 131 49 18 BsiEI CG_ry'CG Cuts at: 0 114 514 1338 Size: 114 400 824 Fragments arranged by size: 824 400 114 BsiHKAI G_wGCw'C Cuts at: 0 83 491 713 998 1338 Size: 83 408 222 285 340 Fragments arranged by size: 408 340 285 222 83 Bsp1286I G_dGCh'C Cuts at: 0 83 324 479 491 545 641 713 892 Size: 83 241 155 12 54 96 72 179 Cuts at: 892 942 998 1113 1205 1256 1269 1276 1292 Size: 50 56 115 92 51 13 7 16 Cuts at: 1292 1338 Size: 46 Fragments arranged by size: 241 179 155 115 96 92 83 72 56 54 51 50 46 16 13 12 7 BspMI ACCTGCnnnn'nnnn_ Cuts at: 0 174 422 474 578 717 728 761 1031 Size: 174 248 52 104 139 11 33 270 Cuts at: 1031 1338 Size: 307 Fragments arranged by size: 307 270 248 174 139 104 52 33 11 BsrBI CCG'CTC Cuts at: 0 785 1017 1338 Size: 785 232 321 Fragments arranged by size: 785 321 232 BssHII G'CGCG_C Cuts at: 0 291 1338 Size: 291 1047 BssSI C'ACGA_G Cuts at: 0 436 1338 Size: 436 902 BstXI CCAn_nnnn'nTGG Cuts at: 0 63 1338 Size: 63 1275 BstYI r'GATC_y Cuts at: 0 605 1338 Size: 605 733 Bsu36I CC'TnA_GG Cuts at: 0 455 1166 1338 Size: 455 711 172 Fragments arranged by size: 711 455 172 BtgI C'CryG_G Cuts at: 0 462 545 1213 1233 1248 1338 Size: 462 83 668 20 15 90 Fragments arranged by size: 668 462 90 83 20 15 BtsI GCAGTG_nn' Cuts at: 0 268 890 1338 Size: 268 622 448 Fragments arranged by size: 622 448 268 DraIII CAC_nnn'GTG Cuts at: 0 1002 1338 Size: 1002 336 EaeI y'GGCC_r Cuts at: 0 1087 1338 Size: 1087 251 EarI CTCTTCn'nnn_ Cuts at: 0 781 1338 Size: 781 557 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 0 169 1087 1233 1338 Size: 169 918 146 105 Fragments arranged by size: 918 169 146 105 EcoICRI GAG'CTC Cuts at: 0 81 1338 Size: 81 1257 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 0 39 398 946 1338 Size: 39 359 548 392 Fragments arranged by size: 548 392 359 39 EcoNI CCTnn'n_nnAGG Cuts at: 0 16 724 832 1338 Size: 16 708 108 506 Fragments arranged by size: 708 506 108 16 EcoO109I rG'GnC_Cy Cuts at: 0 9 21 320 372 411 541 638 908 Size: 9 12 299 52 39 130 97 270 Cuts at: 908 1020 1075 1109 1272 1273 1288 1289 1298 Size: 112 55 34 163 1 15 1 9 Cuts at: 1298 1338 Size: 40 Fragments arranged by size: 299 270 163 130 112 97 55 52 40 39 34 15 12 9 9 1 1 HaeII r_GCGC'y Cuts at: 0 886 1160 1338 Size: 886 274 178 Fragments arranged by size: 886 274 178 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 0 43 75 1338 Size: 43 32 1263 Fragments arranged by size: 1263 43 32 KasI G'GCGC_C Cuts at: 0 1156 1338 Size: 1156 182 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 0 95 717 992 1338 Size: 95 622 275 346 Fragments arranged by size: 622 346 275 95 MspA1I CmG'CkG Cuts at: 0 86 966 1029 1047 1056 1137 1215 1235 Size: 86 880 63 18 9 81 78 20 Cuts at: 1235 1338 Size: 103 Fragments arranged by size: 880 103 86 81 78 63 20 18 9 NarI GG'CG_CC Cuts at: 0 1157 1338 Size: 1157 181 NcoI C'CATG_G Cuts at: 0 1248 1338 Size: 1248 90 PasI CC'CwG_GG Cuts at: 0 12 537 625 1338 Size: 12 525 88 713 Fragments arranged by size: 713 525 88 12 PflMI CCAn_nnn'nTGG Cuts at: 0 12 1137 1338 Size: 12 1125 201 Fragments arranged by size: 1125 201 12 PfoI T'CCnGG_A Cuts at: 0 923 1322 1338 Size: 923 399 16 Fragments arranged by size: 923 399 16 PmlI CAC'GTG Cuts at: 0 708 1338 Size: 708 630 PpuMI rG'GwC_Cy Cuts at: 0 21 411 1020 1075 1338 Size: 21 390 609 55 263 Fragments arranged by size: 609 390 263 55 21 PspOMI G'GGCC_C Cuts at: 0 320 541 637 1109 1265 1272 1288 1338 Size: 320 221 96 472 156 7 16 50 Fragments arranged by size: 472 320 221 156 96 50 16 7 PstI C_TGCA'G Cuts at: 0 47 171 485 725 897 971 1028 1338 Size: 47 124 314 240 172 74 57 310 Fragments arranged by size: 314 310 240 172 124 74 57 47 PvuII CAG'CTG Cuts at: 0 966 1047 1056 1137 1338 Size: 966 81 9 81 201 Fragments arranged by size: 966 201 81 81 9 SacI G_AGCT'C Cuts at: 0 83 1338 Size: 83 1255 SacII CC_GC'GG Cuts at: 0 1216 1236 1338 Size: 1216 20 102 Fragments arranged by size: 1216 102 20 SanDI GG'GwC_CC Cuts at: 0 1075 1338 Size: 1075 263 SbfI CC_TGCA'GG Cuts at: 0 485 1338 Size: 485 853 ScaI AGT'ACT Cuts at: 0 848 1338 Size: 848 490 SexAI A'CCwGG_T Cuts at: 0 23 1338 Size: 23 1315 SfcI C'TryA_G Cuts at: 0 43 167 481 721 893 967 1024 1338 Size: 43 124 314 240 172 74 57 314 Fragments arranged by size: 314 314 240 172 124 74 57 43 SfiI GGCCn_nnn'nGGCC Cuts at: 0 1048 1296 1338 Size: 1048 248 42 Fragments arranged by size: 1048 248 42 SfoI GGC'GCC Cuts at: 0 1158 1338 Size: 1158 180 SmaI CCC'GGG Cuts at: 0 1270 1338 Size: 1270 68 SmlI C'TyrA_G Cuts at: 0 490 1338 Size: 490 848 StuI AGG'CCT Cuts at: 0 496 586 979 1338 Size: 496 90 393 359 Fragments arranged by size: 496 393 359 90 StyI C'CwwG_G Cuts at: 0 1248 1338 Size: 1248 90 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 0 176 189 1338 Size: 176 13 1149 Fragments arranged by size: 1149 176 13 TatI w'GTAC_w Cuts at: 0 846 1338 Size: 846 492 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 0 181 213 1338 Size: 181 32 1125 Fragments arranged by size: 1125 181 32 XcmI CCAnnnn_n'nnnnTGG Cuts at: 0 1320 1338 Size: 1320 18 XmaI C'CCGG_G Cuts at: 0 1268 1338 Size: 1268 70 Enzymes that do cut: AarI AccI AlfI AlwNI ApaI ApaLI AvaI BanI BanII BbeI BcgI BglI BglII BlpI Bme1580I BmrI BplI BpmI BpuEI BsaI BsaAI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI Bsp1286I BspMI BsrBI BssHII BssSI BstXI BstYI Bsu36I BtgI BtsI DraIII EaeI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I HaeII Hin4I KasI MmeI MspA1I NarI NcoI PasI PflMI PfoI PmlI PpuMI PspOMI PstI PvuII SacI SacII SanDI SbfI ScaI SexAI SfcI SfiI SfoI SmaI SmlI StuI StyI TaqII TatI TstI XcmI XmaI Enzymes that do not cut: AatII Acc65I AclI AcuI AfeI AflII AflIII AgeI AhdI AleI AloI ApoI AscI AseI AsiSI AvrII BaeI BamHI BbsI BbvCI BciVI BclI BfrBI BmgBI BmtI Bpu10I BsaBI BsiWI BsmI BsmBI BspEI BspHI BsrDI BsrFI BsrGI BstAPI BstBI BstEII BstZ17I BtgZI ClaI CspCI DraI DrdI EagI EcoRI EcoRV FalI FseI FspI FspAI HincII HindIII HpaI KpnI MfeI MluI MscI MslI NaeI NdeI NgoMIV NheI NotI NruI NsiI NspI PacI PciI PmeI PpiI PshAI PsiI PspXI PsrI PvuI RsrII SalI SapI SgfI SgrAI SnaBI SpeI SphI SrfI SspI SwaI Tth111I XbaI XhoI XmnI ZraI