(Circular) (Six-base) MAPSORT of: pFN21AE4165.seq Check: 1183 from: 1 to: 6550 pFN21AE4165 6550 bp With 182 enzymes: SgfI * July 6, 2012 17:34 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 3123 3123 Size: 6550 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 2313 4360 278 Size: 53 83 186 936 777 2047 2468 Fragments arranged by size: 2468 2047 936 777 186 83 53 AccI GT'mk_AC Cuts at: 1937 2994 3893 1937 Size: 1057 899 4594 Fragments arranged by size: 4594 1057 899 Acc65I G'GTAC_C Cuts at: 3871 3871 Size: 6550 AclI AA'CG_TT Cuts at: 1563 4677 5050 1563 Size: 3114 373 3063 Fragments arranged by size: 3114 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2251 2342 2492 2512 3362 4613 5997 6265 Size: 619 91 150 20 850 1251 1384 268 Cuts at: 6265 1632 Size: 1917 Fragments arranged by size: 1917 1384 1251 850 619 268 150 91 20 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 6353 AflIII A'CryG_T Cuts at: 1203 3063 5449 6426 1203 Size: 1860 2386 977 1327 Fragments arranged by size: 2386 1860 1327 977 AgeI A'CCGG_T Cuts at: 5381 5381 Size: 6550 AhdI GACnn_n'nnGTC Cuts at: 5279 5279 Size: 6550 AleI CACnn'nnGTG Cuts at: 1166 2912 3068 1166 Size: 1746 156 4648 Fragments arranged by size: 4648 1746 156 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 2672 2682 2930 1184 Size: 1488 10 248 4804 Fragments arranged by size: 4804 1488 248 10 AlwNI CAG_nnn'CTG Cuts at: 2161 2440 2806 2845 2872 3115 4293 5865 2161 Size: 279 366 39 27 243 1178 1572 2846 Fragments arranged by size: 2846 1572 1178 366 279 243 39 27 ApaI G_GGCC'C Cuts at: 2066 2072 2110 2066 Size: 6 38 6506 Fragments arranged by size: 6506 38 6 ApaLI G'TGCA_C Cuts at: 2116 4607 5763 2116 Size: 2491 1156 2903 Fragments arranged by size: 2903 2491 1156 ApoI r'AATT_y Cuts at: 1493 3859 4085 4237 6346 1493 Size: 2366 226 152 2109 1697 Fragments arranged by size: 2366 2109 1697 226 152 AseI AT'TA_AT Cuts at: 160 1032 5104 160 Size: 872 4072 1606 Fragments arranged by size: 4072 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6550 AvaI C'yCGr_G Cuts at: 1870 1943 2065 2072 3397 3542 3831 3875 1870 Size: 73 122 7 1325 145 289 44 4545 Fragments arranged by size: 4545 1325 289 145 122 73 44 7 BamHI G'GATC_C Cuts at: 3880 4247 3880 Size: 367 6183 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2128 3478 3518 3871 5326 Size: 325 217 627 341 1350 40 353 1455 Cuts at: 5326 618 Size: 1842 Fragments arranged by size: 1842 1455 1350 627 353 341 325 217 40 BanII G_rGCy'C Cuts at: 1391 2066 2072 2110 3019 3036 3635 3869 1391 Size: 675 6 38 909 17 599 234 4072 Fragments arranged by size: 4072 909 675 599 234 38 17 6 BbeI G_GCGC'C Cuts at: 3482 3482 Size: 6550 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2626 928 Size: 971 727 4852 Fragments arranged by size: 4852 971 727 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4741 4775 1903 Size: 34 2804 34 3678 Fragments arranged by size: 3678 2804 34 34 BciVI GTATCCnnnnn_n' Cuts at: 4442 5652 4442 Size: 1210 5340 BclI T'GATC_A Cuts at: 1548 1548 Size: 6550 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2881 5161 136 Size: 107 122 71 631 605 1209 2280 1525 Fragments arranged by size: 2280 1525 1209 631 605 122 107 71 BglII A'GATC_T Cuts at: 3684 6545 3684 Size: 2861 3689 BlpI GC'TnA_GC Cuts at: 3967 3967 Size: 6550 Bme1580I G_kGCm'C Cuts at: 1790 2066 2072 2110 2120 2931 4611 5767 1790 Size: 276 6 38 10 811 1680 1156 2573 Fragments arranged by size: 2573 1680 1156 811 276 38 10 6 BmgBI CAC'GTC Cuts at: 1318 2410 2827 3580 1318 Size: 1092 417 753 4288 Fragments arranged by size: 4288 1092 753 417 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 4281 5239 442 Size: 416 53 83 399 24 2864 958 1753 Fragments arranged by size: 2864 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6550 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2642 2870 3695 5210 1239 Size: 1403 228 825 1515 2579 Fragments arranged by size: 2579 1515 1403 825 228 Bpu10I CC'TnA_GC Cuts at: 2178 3006 2178 Size: 828 5722 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2096 4675 5540 5838 2096 Size: 2579 865 298 2808 Fragments arranged by size: 2808 2579 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2486 5213 882 Size: 617 987 2727 2219 Fragments arranged by size: 2727 2219 987 617 BsaAI yAC'GTr Cuts at: 493 1204 2743 3066 3097 3562 4341 493 Size: 711 1539 323 31 465 779 2702 Fragments arranged by size: 2702 1539 779 711 465 323 31 BsaBI GATnn'nnATC Cuts at: 3689 6544 3689 Size: 2855 3695 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2310 3147 3479 3712 Size: 53 83 186 936 777 837 332 233 Cuts at: 3712 4357 4739 5374 275 Size: 645 382 635 1451 Fragments arranged by size: 1451 936 837 777 645 635 382 332 233 186 83 53 BsaWI w'CCGG_w Cuts at: 4983 5381 5655 5802 6213 4983 Size: 398 274 147 411 5320 Fragments arranged by size: 5320 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 6325 Fragments arranged by size: 6325 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2180 2477 2609 2612 1186 Size: 994 297 132 3 5124 Fragments arranged by size: 5124 994 297 132 3 BseYI C'CCAG_C Cuts at: 2132 2413 3365 5753 6249 2132 Size: 281 952 2388 496 2433 Fragments arranged by size: 2433 2388 952 496 281 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2214 2423 2819 3422 3764 1163 Size: 573 478 209 396 603 342 3949 Fragments arranged by size: 3949 603 573 478 396 342 209 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2094 2424 2694 2729 2974 3265 Size: 275 186 93 330 270 35 245 291 Cuts at: 3265 3529 4000 4761 5789 1540 Size: 264 471 761 1028 2301 Fragments arranged by size: 2301 1028 761 471 330 291 275 270 264 245 186 93 35 BsiHKAI G_wGCw'C Cuts at: 2120 2748 3036 4611 4696 5767 2120 Size: 628 288 1575 85 1071 2903 Fragments arranged by size: 2903 1575 1071 628 288 85 BsmI GAATG_Cn' Cuts at: 4061 4154 4061 Size: 93 6457 BsmBI CGTCTCn'nnnn_ Cuts at: 3602 3719 3602 Size: 117 6433 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2066 2072 2110 2120 2748 2931 3019 Size: 399 276 6 38 10 628 183 88 Cuts at: 3019 3036 3635 3869 4611 4696 5767 1391 Size: 17 599 234 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 628 599 399 276 234 183 88 85 38 17 10 6 BspEI T'CCGG_A Cuts at: 6213 6213 Size: 6550 BspHI T'CATG_A Cuts at: 4439 6169 4439 Size: 1730 4820 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2504 3123 3786 3905 844 Size: 1660 619 663 119 3489 Fragments arranged by size: 3489 1660 663 619 119 BsrBI CCG'CTC Cuts at: 1394 1823 2767 2976 3298 3772 4437 1394 Size: 429 944 209 322 474 665 3507 Fragments arranged by size: 3507 944 665 474 429 322 209 BsrDI GCAATG_nn' Cuts at: 65 1248 3322 5045 5219 65 Size: 1183 2074 1723 174 1396 Fragments arranged by size: 2074 1723 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2282 2652 3275 3521 3644 3659 5194 Size: 110 472 370 623 246 123 15 1535 Cuts at: 5194 5381 1700 Size: 187 2869 Fragments arranged by size: 2869 1535 623 472 370 246 187 123 110 15 BsrGI T'GTAC_A Cuts at: 96 3744 96 Size: 3648 2902 BssHII G'CGCG_C Cuts at: 1925 2731 1925 Size: 806 5744 BssSI C'ACGA_G Cuts at: 2888 4610 5622 2888 Size: 1722 1012 3816 Fragments arranged by size: 3816 1722 1012 BstAPI GCAn_nnn'nTGC Cuts at: 794 2410 2440 2581 2893 3115 3199 3810 794 Size: 1616 30 141 312 222 84 611 3534 Fragments arranged by size: 3534 1616 611 312 222 141 84 30 BstBI TT'CG_AA Cuts at: 5354 5354 Size: 6550 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 6550 BstXI CCAn_nnnn'nTGG Cuts at: 2488 2488 Size: 6550 BstYI r'GATC_y Cuts at: 1972 3411 3684 3880 4247 4632 4649 6101 6187 Size: 1439 273 196 367 385 17 1452 86 Cuts at: 6187 6199 6545 1972 Size: 12 346 1977 Fragments arranged by size: 1977 1452 1439 385 367 346 273 196 86 17 12 Bsu36I CC'TnA_GG Cuts at: 3376 3376 Size: 6550 BtgI C'CryG_G Cuts at: 513 1065 2004 2338 2445 3255 3291 3453 3510 Size: 552 939 334 107 810 36 162 57 Cuts at: 3510 513 Size: 3553 Fragments arranged by size: 3553 939 810 552 334 162 107 57 36 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5811 Fragments arranged by size: 5811 683 56 BtsI GCAGTG_nn' Cuts at: 3147 3439 4066 4860 4880 3147 Size: 292 627 794 20 4817 Fragments arranged by size: 4817 794 627 292 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6550 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6515 DraI TTT'AAA Cuts at: 3854 3854 Size: 6550 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 2978 5557 809 Size: 507 1662 2579 1802 Fragments arranged by size: 2579 1802 1662 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2091 2163 2421 Size: 54 1425 50 275 18 261 72 258 Cuts at: 2421 2478 2509 2517 2691 2726 3072 3099 3262 Size: 57 31 8 174 35 346 27 163 Cuts at: 3262 3456 3526 3646 3997 4886 6515 8 Size: 194 70 120 351 889 1629 43 Fragments arranged by size: 1629 1425 889 351 346 275 261 258 194 174 163 120 72 70 57 54 50 43 35 31 27 18 8 EagI C'GGCC_G Cuts at: 1537 1812 2091 2421 2691 2726 3262 3526 3997 Size: 275 279 330 270 35 536 264 471 Cuts at: 3997 1537 Size: 4090 Fragments arranged by size: 4090 536 471 330 279 275 270 264 35 EarI CTCTTCn'nnn_ Cuts at: 1353 4480 1353 Size: 3127 3423 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2024 5137 5511 5657 387 Size: 1064 138 435 3113 374 146 1280 Fragments arranged by size: 3113 1280 1064 435 374 146 138 EcoICRI GAG'CTC Cuts at: 3034 3034 Size: 6550 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2251 2342 2492 2512 2642 2870 3362 Size: 393 619 91 150 20 130 228 492 Cuts at: 3362 3695 4613 5210 5997 6265 1239 Size: 333 918 597 787 268 1524 Fragments arranged by size: 1524 918 787 619 597 492 393 333 268 228 150 130 91 20 EcoNI CCTnn'n_nnAGG Cuts at: 2153 2153 Size: 6550 EcoO109I rG'GnC_Cy Cuts at: 2069 2106 2107 2158 3433 3775 2069 Size: 37 1 51 1275 342 4844 Fragments arranged by size: 4844 1275 342 51 37 1 EcoRI G'AATT_C Cuts at: 3859 4237 3859 Size: 378 6172 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2137 2137 Size: 6550 FseI GG_CCGG'CC Cuts at: 3648 3648 Size: 6550 FspI TGC'GCA Cuts at: 5056 5056 Size: 6550 HaeII r_GCGC'y Cuts at: 1678 2052 2080 2376 2629 3349 3482 5697 1678 Size: 374 28 296 253 720 133 2215 2531 Fragments arranged by size: 2531 2215 720 374 296 253 133 28 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2882 2884 2914 2915 2916 Size: 32 135 32 1310 2 30 1 1 Cuts at: 2916 2947 5191 5223 5265 5297 1373 Size: 31 2244 32 42 32 2626 Fragments arranged by size: 2626 2244 1310 135 42 32 32 32 32 31 30 2 1 1 HincII GTy'rAC Cuts at: 669 1657 1938 3894 4140 669 Size: 988 281 1956 246 3079 Fragments arranged by size: 3079 1956 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 6550 HpaI GTT'AAC Cuts at: 4140 4140 Size: 6550 KasI G'GCGC_C Cuts at: 3478 3478 Size: 6550 KpnI G_GTAC'C Cuts at: 3875 3875 Size: 6550 MfeI C'AATT_G Cuts at: 4149 4149 Size: 6550 MluI A'CGCG_T Cuts at: 6426 6426 Size: 6550 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 5664 5848 1305 Size: 4359 184 2007 Fragments arranged by size: 4359 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2165 2519 10 Size: 54 1425 343 333 354 4041 Fragments arranged by size: 4041 1425 354 343 333 54 MslI CAynn'nnrTG Cuts at: 518 1166 2486 2816 2912 3041 3068 4508 4867 Size: 648 1320 330 96 129 27 1440 359 Cuts at: 4867 5026 518 Size: 159 2042 Fragments arranged by size: 2042 1440 1320 648 359 330 159 129 96 27 MspA1I CmG'CkG Cuts at: 1606 2028 2700 3001 3129 3226 3570 3966 4643 Size: 422 672 301 128 97 344 396 677 Cuts at: 4643 5791 6036 1606 Size: 1148 245 2120 Fragments arranged by size: 2120 1148 677 672 422 396 344 301 245 128 97 NaeI GCC'GGC Cuts at: 3277 3523 3646 3661 3277 Size: 246 123 15 6166 Fragments arranged by size: 6166 246 123 15 NarI GG'CG_CC Cuts at: 3479 3479 Size: 6550 NcoI C'CATG_G Cuts at: 513 1065 2004 3510 513 Size: 552 939 1506 3553 Fragments arranged by size: 3553 1506 939 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 6550 NgoMIV G'CCGG_C Cuts at: 3275 3521 3644 3659 3275 Size: 246 123 15 6166 Fragments arranged by size: 6166 246 123 15 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6550 NotI GC'GGCC_GC Cuts at: 2726 3262 3526 3997 2726 Size: 536 264 471 5279 Fragments arranged by size: 5279 536 471 264 NspI r_CATG'y Cuts at: 1132 1229 2293 3908 5453 1132 Size: 97 1064 1615 1545 2229 Fragments arranged by size: 2229 1615 1545 1064 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 6550 PciI A'CATG_T Cuts at: 5449 5449 Size: 6550 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 6550 PfoI T'CCnGG_A Cuts at: 3786 3786 Size: 6550 PmeI GTTT'AAAC Cuts at: 3854 3854 Size: 6550 PmlI CAC'GTG Cuts at: 2743 3066 3562 2743 Size: 323 496 5731 Fragments arranged by size: 5731 496 323 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4619 4651 6132 6164 4619 Size: 32 1481 32 5005 Fragments arranged by size: 5005 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 3775 3775 Size: 6550 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6550 PsiI TTA'TAA Cuts at: 4120 4120 Size: 6550 PspOMI G'GGCC_C Cuts at: 2062 2068 2106 2062 Size: 6 38 6506 Fragments arranged by size: 6506 38 6 PstI C_TGCA'G Cuts at: 830 2157 2258 2586 2646 2661 2709 3059 3120 Size: 1327 101 328 60 15 48 350 61 Cuts at: 3120 3195 3902 830 Size: 75 707 3478 Fragments arranged by size: 3478 1327 707 350 328 101 75 61 60 48 15 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6550 PvuII CAG'CTG Cuts at: 3001 3226 3001 Size: 225 6325 SacI G_AGCT'C Cuts at: 3036 3036 Size: 6550 SalI G'TCGA_C Cuts at: 1936 3892 1936 Size: 1956 4594 SbfI CC_TGCA'GG Cuts at: 2157 3195 3902 2157 Size: 1038 707 4805 Fragments arranged by size: 4805 1038 707 ScaI AGT'ACT Cuts at: 3596 4798 6510 3596 Size: 1202 1712 3636 Fragments arranged by size: 3636 1712 1202 SexAI A'CCwGG_T Cuts at: 2865 2865 Size: 6550 SfcI C'TryA_G Cuts at: 826 1045 2153 2254 2368 2582 2642 2657 2705 Size: 219 1108 101 114 214 60 15 48 Cuts at: 2705 3055 3116 3191 3898 5033 5714 5905 826 Size: 350 61 75 707 1135 681 191 1471 Fragments arranged by size: 1471 1135 1108 707 681 350 219 214 191 114 101 75 61 60 48 15 SfoI GGC'GCC Cuts at: 3480 3480 Size: 6550 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6550 SgrAI Cr'CCGG_yG Cuts at: 1700 2282 1700 Size: 582 5968 SmaI CCC'GGG Cuts at: 1872 2067 3833 3877 1872 Size: 195 1766 44 4545 Fragments arranged by size: 4545 1766 195 44 SmlI C'TyrA_G Cuts at: 820 1017 1943 2111 4654 5555 5817 820 Size: 197 926 168 2543 901 262 1553 Fragments arranged by size: 2543 1553 926 901 262 197 168 SnaBI TAC'GTA Cuts at: 493 4341 493 Size: 3848 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6550 SphI G_CATG'C Cuts at: 1132 2293 3908 1132 Size: 1161 1615 3774 Fragments arranged by size: 3774 1615 1161 SrfI GCCC'GGGC Cuts at: 2067 2067 Size: 6550 SspI AAT'ATT Cuts at: 5 52 4474 5 Size: 47 4422 2081 Fragments arranged by size: 4422 2081 47 StuI AGG'CCT Cuts at: 1517 2222 2855 1517 Size: 705 633 5212 Fragments arranged by size: 5212 705 633 StyI C'CwwG_G Cuts at: 513 1065 2004 3510 3989 513 Size: 552 939 1506 479 3074 Fragments arranged by size: 3074 1506 939 552 479 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2146 2968 3506 4577 4594 4747 4932 Size: 1255 566 822 538 1071 17 153 185 Cuts at: 4932 5377 325 Size: 445 1498 Fragments arranged by size: 1498 1255 1071 822 566 538 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 3594 3744 4796 Size: 274 80 33 51 1443 1617 150 1052 Cuts at: 4796 6508 96 Size: 1712 138 Fragments arranged by size: 1712 1617 1443 1052 274 150 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2816 2848 6510 6542 2816 Size: 32 3662 32 2824 Fragments arranged by size: 3662 2824 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 6550 XbaI T'CTAG_A Cuts at: 3886 3886 Size: 6550 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2530 2530 Size: 6550 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6550 XmaI C'CCGG_G Cuts at: 1870 2065 3831 3875 1870 Size: 195 1766 44 4545 Fragments arranged by size: 4545 1766 195 44 XmnI GAAnn'nnTTC Cuts at: 4679 4679 Size: 6550 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 2311 4358 276 Size: 53 83 186 936 777 2047 2468 Fragments arranged by size: 2468 2047 936 777 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI FseI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PfoI PmeI PmlI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI PvuII SacI SalI SbfI ScaI SexAI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SrfI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI AloI AscI AvrII BaeI BbvCI BfrBI BplI BsiWI BstZ17I DraIII EcoRV FspAI NruI NsiI PacI PspXI PsrI RsrII SacII SanDI SapI SfiI SwaI