(Linear) (Six-base) MAPSORT of: KIEE4165.seq Check: 7935 from: 1 to: 1845 KIEE4165.orf With 182 enzymes: SgfI * July 3, 2012 18:41 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 0 1118 1845 Size: 1118 727 AatII G_ACGT'C Cuts at: 0 308 1845 Size: 308 1537 AccI GT'mk_AC Cuts at: 0 989 1845 Size: 989 856 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 0 246 337 487 507 1357 1845 Size: 246 91 150 20 850 488 Fragments arranged by size: 850 488 246 150 91 20 AflIII A'CryG_T Cuts at: 0 1058 1845 Size: 1058 787 AleI CACnn'nnGTG Cuts at: 0 907 1063 1845 Size: 907 156 782 Fragments arranged by size: 907 782 156 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 0 667 677 925 1845 Size: 667 10 248 920 Fragments arranged by size: 920 667 248 10 AlwNI CAG_nnn'CTG Cuts at: 0 156 435 801 840 867 1110 1845 Size: 156 279 366 39 27 243 735 Fragments arranged by size: 735 366 279 243 156 39 27 ApaI G_GGCC'C Cuts at: 0 61 67 105 1845 Size: 61 6 38 1740 Fragments arranged by size: 1740 61 38 6 ApaLI G'TGCA_C Cuts at: 0 111 1845 Size: 111 1734 AvaI C'yCGr_G Cuts at: 0 60 67 1392 1537 1826 1845 Size: 60 7 1325 145 289 19 Fragments arranged by size: 1325 289 145 60 19 7 BanI G'GyrC_C Cuts at: 0 123 1473 1513 1845 Size: 123 1350 40 332 Fragments arranged by size: 1350 332 123 40 BanII G_rGCy'C Cuts at: 0 61 67 105 1014 1031 1630 1845 Size: 61 6 38 909 17 599 215 Fragments arranged by size: 909 599 215 61 38 17 6 BbeI G_GCGC'C Cuts at: 0 1477 1845 Size: 1477 368 BbsI GAAGACnn'nnnn_ Cuts at: 0 621 1845 Size: 621 1224 BglI GCCn_nnn'nGGC Cuts at: 0 876 1845 Size: 876 969 BglII A'GATC_T Cuts at: 0 1679 1845 Size: 1679 166 Bme1580I G_kGCm'C Cuts at: 0 61 67 105 115 926 1845 Size: 61 6 38 10 811 919 Fragments arranged by size: 919 811 61 38 10 6 BmgBI CAC'GTC Cuts at: 0 405 822 1575 1845 Size: 405 417 753 270 Fragments arranged by size: 753 417 405 270 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 637 865 1690 1845 Size: 637 228 825 155 Fragments arranged by size: 825 637 228 155 Bpu10I CC'TnA_GC Cuts at: 0 173 1001 1845 Size: 173 828 844 Fragments arranged by size: 844 828 173 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 91 1845 Size: 91 1754 BsaI GGTCTCn'nnnn_ Cuts at: 0 481 1845 Size: 481 1364 BsaAI yAC'GTr Cuts at: 0 738 1061 1092 1557 1845 Size: 738 323 31 465 288 Fragments arranged by size: 738 465 323 288 31 BsaBI GATnn'nnATC Cuts at: 0 1684 1845 Size: 1684 161 BsaHI Gr'CG_yC Cuts at: 0 305 1142 1474 1707 1845 Size: 305 837 332 233 138 Fragments arranged by size: 837 332 305 233 138 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 0 175 472 604 607 1845 Size: 175 297 132 3 1238 Fragments arranged by size: 1238 297 175 132 3 BseYI C'CCAG_C Cuts at: 0 127 408 1360 1845 Size: 127 281 952 485 Fragments arranged by size: 952 485 281 127 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 0 209 418 814 1417 1759 1845 Size: 209 209 396 603 342 86 Fragments arranged by size: 603 396 342 209 209 86 BsiEI CG_ry'CG Cuts at: 0 89 419 689 724 969 1260 1524 1845 Size: 89 330 270 35 245 291 264 321 Fragments arranged by size: 330 321 291 270 264 245 89 35 BsiHKAI G_wGCw'C Cuts at: 0 115 743 1031 1845 Size: 115 628 288 814 Fragments arranged by size: 814 628 288 115 BsmBI CGTCTCn'nnnn_ Cuts at: 0 1597 1714 1845 Size: 1597 117 131 Fragments arranged by size: 1597 131 117 Bsp1286I G_dGCh'C Cuts at: 0 61 67 105 115 743 926 1014 1031 Size: 61 6 38 10 628 183 88 17 Cuts at: 1031 1630 1845 Size: 599 215 Fragments arranged by size: 628 599 215 183 88 61 38 17 10 6 BspMI ACCTGCnnnn'nnnn_ Cuts at: 0 499 1118 1781 1845 Size: 499 619 663 64 Fragments arranged by size: 663 619 499 64 BsrBI CCG'CTC Cuts at: 0 762 971 1293 1767 1845 Size: 762 209 322 474 78 Fragments arranged by size: 762 474 322 209 78 BsrDI GCAATG_nn' Cuts at: 0 1317 1845 Size: 1317 528 BsrFI r'CCGG_y Cuts at: 0 277 647 1270 1516 1639 1654 1845 Size: 277 370 623 246 123 15 191 Fragments arranged by size: 623 370 277 246 191 123 15 BsrGI T'GTAC_A Cuts at: 0 1739 1845 Size: 1739 106 BssHII G'CGCG_C Cuts at: 0 726 1845 Size: 726 1119 BssSI C'ACGA_G Cuts at: 0 883 1845 Size: 883 962 BstAPI GCAn_nnn'nTGC Cuts at: 0 405 435 576 888 1110 1194 1805 1845 Size: 405 30 141 312 222 84 611 40 Fragments arranged by size: 611 405 312 222 141 84 40 30 BstXI CCAn_nnnn'nTGG Cuts at: 0 483 1845 Size: 483 1362 BstYI r'GATC_y Cuts at: 0 1406 1679 1845 Size: 1406 273 166 Fragments arranged by size: 1406 273 166 Bsu36I CC'TnA_GG Cuts at: 0 1371 1845 Size: 1371 474 BtgI C'CryG_G Cuts at: 0 333 440 1250 1286 1448 1505 1845 Size: 333 107 810 36 162 57 340 Fragments arranged by size: 810 340 333 162 107 57 36 BtsI GCAGTG_nn' Cuts at: 0 1142 1434 1845 Size: 1142 292 411 Fragments arranged by size: 1142 411 292 DrdI GACnn_nn'nnGTC Cuts at: 0 973 1845 Size: 973 872 EaeI y'GGCC_r Cuts at: 0 86 158 416 473 504 512 686 721 Size: 86 72 258 57 31 8 174 35 Cuts at: 721 1067 1094 1257 1451 1521 1641 1845 Size: 346 27 163 194 70 120 204 Fragments arranged by size: 346 258 204 194 174 163 120 86 72 70 57 35 31 27 8 EagI C'GGCC_G Cuts at: 0 86 416 686 721 1257 1521 1845 Size: 86 330 270 35 536 264 324 Fragments arranged by size: 536 330 324 270 264 86 35 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 0 19 1845 Size: 19 1826 EcoICRI GAG'CTC Cuts at: 0 1029 1845 Size: 1029 816 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 0 246 337 487 507 637 865 1357 1690 Size: 246 91 150 20 130 228 492 333 Cuts at: 1690 1845 Size: 155 Fragments arranged by size: 492 333 246 228 155 150 130 91 20 EcoNI CCTnn'n_nnAGG Cuts at: 0 148 1845 Size: 148 1697 EcoO109I rG'GnC_Cy Cuts at: 0 64 101 102 153 1428 1770 1845 Size: 64 37 1 51 1275 342 75 Fragments arranged by size: 1275 342 75 64 51 37 1 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 0 132 1845 Size: 132 1713 FseI GG_CCGG'CC Cuts at: 0 1643 1845 Size: 1643 202 HaeII r_GCGC'y Cuts at: 0 47 75 371 624 1344 1477 1845 Size: 47 28 296 253 720 133 368 Fragments arranged by size: 720 368 296 253 133 47 28 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 0 877 879 909 910 911 942 1845 Size: 877 2 30 1 1 31 903 Fragments arranged by size: 903 877 31 30 2 1 1 KasI G'GCGC_C Cuts at: 0 1473 1845 Size: 1473 372 MscI TGG'CCA Cuts at: 0 160 514 1845 Size: 160 354 1331 Fragments arranged by size: 1331 354 160 MslI CAynn'nnrTG Cuts at: 0 481 811 907 1036 1063 1845 Size: 481 330 96 129 27 782 Fragments arranged by size: 782 481 330 129 96 27 MspA1I CmG'CkG Cuts at: 0 23 695 996 1124 1221 1565 1845 Size: 23 672 301 128 97 344 280 Fragments arranged by size: 672 344 301 280 128 97 23 NaeI GCC'GGC Cuts at: 0 1272 1518 1641 1656 1845 Size: 1272 246 123 15 189 Fragments arranged by size: 1272 246 189 123 15 NarI GG'CG_CC Cuts at: 0 1474 1845 Size: 1474 371 NcoI C'CATG_G Cuts at: 0 1505 1845 Size: 1505 340 NgoMIV G'CCGG_C Cuts at: 0 1270 1516 1639 1654 1845 Size: 1270 246 123 15 191 Fragments arranged by size: 1270 246 191 123 15 NotI GC'GGCC_GC Cuts at: 0 721 1257 1521 1845 Size: 721 536 264 324 Fragments arranged by size: 721 536 324 264 NspI r_CATG'y Cuts at: 0 288 1845 Size: 288 1557 PfoI T'CCnGG_A Cuts at: 0 1781 1845 Size: 1781 64 PmlI CAC'GTG Cuts at: 0 738 1061 1557 1845 Size: 738 323 496 288 Fragments arranged by size: 738 496 323 288 PpuMI rG'GwC_Cy Cuts at: 0 1770 1845 Size: 1770 75 PspOMI G'GGCC_C Cuts at: 0 57 63 101 1845 Size: 57 6 38 1744 Fragments arranged by size: 1744 57 38 6 PstI C_TGCA'G Cuts at: 0 152 253 581 641 656 704 1054 1115 Size: 152 101 328 60 15 48 350 61 Cuts at: 1115 1190 1845 Size: 75 655 Fragments arranged by size: 655 350 328 152 101 75 61 60 48 15 PvuII CAG'CTG Cuts at: 0 996 1221 1845 Size: 996 225 624 Fragments arranged by size: 996 624 225 SacI G_AGCT'C Cuts at: 0 1031 1845 Size: 1031 814 SbfI CC_TGCA'GG Cuts at: 0 152 1190 1845 Size: 152 1038 655 Fragments arranged by size: 1038 655 152 ScaI AGT'ACT Cuts at: 0 1591 1845 Size: 1591 254 SexAI A'CCwGG_T Cuts at: 0 860 1845 Size: 860 985 SfcI C'TryA_G Cuts at: 0 148 249 363 577 637 652 700 1050 Size: 148 101 114 214 60 15 48 350 Cuts at: 1050 1111 1186 1845 Size: 61 75 659 Fragments arranged by size: 659 350 214 148 114 101 75 61 60 48 15 SfoI GGC'GCC Cuts at: 0 1475 1845 Size: 1475 370 SgrAI Cr'CCGG_yG Cuts at: 0 277 1845 Size: 277 1568 SmaI CCC'GGG Cuts at: 0 62 1828 1845 Size: 62 1766 17 Fragments arranged by size: 1766 62 17 SmlI C'TyrA_G Cuts at: 0 106 1845 Size: 106 1739 SphI G_CATG'C Cuts at: 0 288 1845 Size: 288 1557 SrfI GCCC'GGGC Cuts at: 0 62 1845 Size: 62 1783 StuI AGG'CCT Cuts at: 0 217 850 1845 Size: 217 633 995 Fragments arranged by size: 995 633 217 StyI C'CwwG_G Cuts at: 0 1505 1845 Size: 1505 340 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 0 141 963 1501 1845 Size: 141 822 538 344 Fragments arranged by size: 822 538 344 141 TatI w'GTAC_w Cuts at: 0 1589 1739 1845 Size: 1589 150 106 Fragments arranged by size: 1589 150 106 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 0 811 843 1845 Size: 811 32 1002 Fragments arranged by size: 1002 811 32 XcmI CCAnnnn_n'nnnnTGG Cuts at: 0 525 1845 Size: 525 1320 XmaI C'CCGG_G Cuts at: 0 60 1826 1845 Size: 60 1766 19 Fragments arranged by size: 1766 60 19 ZraI GAC'GTC Cuts at: 0 306 1845 Size: 306 1539 Enzymes that do cut: AarI AatII AccI AcuI AflIII AleI AlfI AlwNI ApaI ApaLI AvaI BanI BanII BbeI BbsI BglI BglII Bme1580I BmgBI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BseRI BseYI BsgI BsiEI BsiHKAI BsmBI Bsp1286I BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstXI BstYI Bsu36I BtgI BtsI DrdI EaeI EagI EciI EcoICRI Eco57MI EcoNI EcoO109I FalI FseI HaeII Hin4I KasI MscI MslI MspA1I NaeI NarI NcoI NgoMIV NotI NspI PfoI PmlI PpuMI PspOMI PstI PvuII SacI SbfI ScaI SexAI SfcI SfoI SgrAI SmaI SmlI SphI SrfI StuI StyI TaqII TatI TstI XcmI XmaI ZraI Enzymes that do not cut: Acc65I AclI AfeI AflII AgeI AhdI AloI ApoI AscI AseI AsiSI AvrII BaeI BamHI BbvCI BcgI BciVI BclI BfrBI BlpI BmrI BmtI BplI BsaWI BsaXI BsiWI BsmI BspEI BspHI BstBI BstEII BstZ17I BtgZI ClaI CspCI DraI DraIII EarI EcoRI EcoRV FspI FspAI HincII HindIII HpaI KpnI MfeI MluI MmeI NdeI NheI NruI NsiI PacI PasI PciI PflMI PmeI PpiI PshAI PsiI PspXI PsrI PvuI RsrII SacII SalI SanDI SapI SfiI SgfI SnaBI SpeI SspI SwaI Tth111I XbaI XhoI XmnI