(Circular) (Six-base) MAPSORT of: pFN21AE9627.seq Check: 3086 from: 1 to: 7288 pFN21AE9627 7288 bp With 182 enzymes: SgfI * July 6, 2012 17:34 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 5098 278 Size: 53 83 186 936 3562 2468 Fragments arranged by size: 3562 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 4631 1937 Size: 2694 4594 Acc65I G'GTAC_C Cuts at: 4609 4609 Size: 7288 AclI AA'CG_TT Cuts at: 1563 5415 5788 1563 Size: 3852 373 3063 Fragments arranged by size: 3852 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2310 2562 2759 3039 3639 3893 4256 4433 Size: 678 252 197 280 600 254 363 177 Cuts at: 4433 5351 6735 7003 1632 Size: 918 1384 268 1917 Fragments arranged by size: 1917 1384 918 678 600 363 280 268 254 252 197 177 AfeI AGC'GCT Cuts at: 4433 4433 Size: 7288 AflII C'TTAA_G Cuts at: 820 1017 3628 820 Size: 197 2611 4480 Fragments arranged by size: 4480 2611 197 AflIII A'CryG_T Cuts at: 1203 6187 7164 1203 Size: 4984 977 1327 Fragments arranged by size: 4984 1327 977 AgeI A'CCGG_T Cuts at: 6119 6119 Size: 7288 AhdI GACnn_n'nnGTC Cuts at: 2373 6017 2373 Size: 3644 3644 AleI CACnn'nnGTG Cuts at: 1166 3396 1166 Size: 2230 5058 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 7288 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2837 2869 2837 Size: 32 7256 AlwNI CAG_nnn'CTG Cuts at: 3140 4015 5031 6603 3140 Size: 875 1016 1572 3825 Fragments arranged by size: 3825 1572 1016 875 ApaI G_GGCC'C Cuts at: 2044 3119 2044 Size: 1075 6213 ApaLI G'TGCA_C Cuts at: 3456 5345 6501 3456 Size: 1889 1156 4243 Fragments arranged by size: 4243 1889 1156 ApoI r'AATT_y Cuts at: 1493 2674 2885 3369 4597 4823 4975 7084 1493 Size: 1181 211 484 1228 226 152 2109 1697 Fragments arranged by size: 2109 1697 1228 1181 484 226 211 152 AseI AT'TA_AT Cuts at: 160 1032 3464 5842 160 Size: 872 2432 2378 1606 Fragments arranged by size: 2432 2378 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 7288 AvaI C'yCGr_G Cuts at: 1870 1943 2019 4613 1870 Size: 73 76 2594 4545 Fragments arranged by size: 4545 2594 76 73 AvrII C'CTAG_G Cuts at: 2108 2108 Size: 7288 BamHI G'GATC_C Cuts at: 4618 4985 4618 Size: 367 6921 BanI G'GyrC_C Cuts at: 618 943 1160 1787 4609 6064 618 Size: 325 217 627 2822 1455 1842 Fragments arranged by size: 2822 1842 1455 627 325 217 BanII G_rGCy'C Cuts at: 1391 2020 2044 2075 3119 3162 3852 4029 4607 Size: 629 24 31 1044 43 690 177 578 Cuts at: 4607 1391 Size: 4072 Fragments arranged by size: 4072 1044 690 629 578 177 43 31 24 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2440 4273 928 Size: 971 541 1833 3943 Fragments arranged by size: 3943 1833 971 541 BbvCI CC'TCA_GC Cuts at: 2115 2115 Size: 7288 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 5479 5513 1903 Size: 34 3542 34 3678 Fragments arranged by size: 3678 3542 34 34 BciVI GTATCCnnnnn_n' Cuts at: 5180 6390 5180 Size: 1210 6078 BclI T'GATC_A Cuts at: 1548 1548 Size: 7288 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 5899 136 Size: 107 122 71 631 605 4227 1525 Fragments arranged by size: 4227 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 2467 7283 2467 Size: 4816 2472 BlpI GC'TnA_GC Cuts at: 4705 4705 Size: 7288 Bme1580I G_kGCm'C Cuts at: 1790 2044 3119 3460 5349 6505 1790 Size: 254 1075 341 1889 1156 2573 Fragments arranged by size: 2573 1889 1156 1075 341 254 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 7288 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2019 2374 2983 Size: 416 53 83 399 24 602 355 609 Cuts at: 2983 3334 5019 5977 442 Size: 351 1685 958 1753 Fragments arranged by size: 1753 1685 958 609 602 416 399 355 351 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 7288 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3866 3866 Size: 7288 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2183 3228 3874 4281 5948 1239 Size: 944 1045 646 407 1667 2579 Fragments arranged by size: 2579 1667 1045 944 646 407 Bpu10I CC'TnA_GC Cuts at: 2115 3156 2115 Size: 1041 6247 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 5413 6278 6576 5413 Size: 865 298 6125 Fragments arranged by size: 6125 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 5951 882 Size: 617 4452 2219 Fragments arranged by size: 4452 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 5079 493 Size: 711 3875 2702 Fragments arranged by size: 3875 2702 711 BsaBI GATnn'nnATC Cuts at: 2585 3417 7282 2585 Size: 832 3865 2591 Fragments arranged by size: 3865 2591 832 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 5095 5477 6112 275 Size: 53 83 186 936 3562 382 635 1451 Fragments arranged by size: 3562 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 5721 6119 6393 6540 6951 5721 Size: 398 274 147 411 6058 Fragments arranged by size: 6058 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2181 2211 2213 2243 2559 Size: 30 165 30 778 30 2 30 316 Cuts at: 2559 2589 1178 Size: 30 5877 Fragments arranged by size: 5877 778 316 165 30 30 30 30 30 2 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2256 3858 1186 Size: 1070 1602 4616 Fragments arranged by size: 4616 1602 1070 BseYI C'CCAG_C Cuts at: 3920 4085 6491 6987 3920 Size: 165 2406 496 4221 Fragments arranged by size: 4221 2406 496 165 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2785 1163 Size: 573 1049 5666 Fragments arranged by size: 5666 1049 573 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 4738 5499 6527 1540 Size: 275 186 2737 761 1028 2301 Fragments arranged by size: 2737 2301 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2996 3162 3460 3852 5349 5434 6505 2996 Size: 166 298 392 1497 85 1071 3779 Fragments arranged by size: 3779 1497 1071 392 298 166 85 BsmI GAATG_Cn' Cuts at: 4799 4892 4799 Size: 93 7195 BsmBI CGTCTCn'nnnn_ Cuts at: 4113 4113 Size: 7288 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2020 2044 2075 2996 3119 3162 3460 Size: 399 230 24 31 921 123 43 298 Cuts at: 3460 3852 4029 4607 5349 5434 6505 1391 Size: 392 177 578 742 85 1071 2174 Fragments arranged by size: 2174 1071 921 742 578 399 392 298 230 177 123 85 43 31 24 BspEI T'CCGG_A Cuts at: 6951 6951 Size: 7288 BspHI T'CATG_A Cuts at: 2263 2670 5177 6907 2263 Size: 407 2507 1730 2644 Fragments arranged by size: 2644 2507 1730 407 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 4643 844 Size: 3799 3489 BsrBI CCG'CTC Cuts at: 1394 1823 5175 1394 Size: 429 3352 3507 Fragments arranged by size: 3507 3352 429 BsrDI GCAATG_nn' Cuts at: 65 1248 3801 4078 5783 5957 65 Size: 1183 2553 277 1705 174 1396 Fragments arranged by size: 2553 1705 1396 1183 277 174 BsrFI r'CCGG_y Cuts at: 1700 1810 5932 6119 1700 Size: 110 4122 187 2869 Fragments arranged by size: 4122 2869 187 110 BsrGI T'GTAC_A Cuts at: 96 3731 96 Size: 3635 3653 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 7288 BssSI C'ACGA_G Cuts at: 3878 5348 6360 3878 Size: 1470 1012 4806 Fragments arranged by size: 4806 1470 1012 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 7288 BstBI TT'CG_AA Cuts at: 2827 6092 2827 Size: 3265 4023 BstEII G'GTnAC_C Cuts at: 1184 4309 1184 Size: 3125 4163 BstYI r'GATC_y Cuts at: 1972 2467 4618 4985 5370 5387 6839 6925 6937 Size: 495 2151 367 385 17 1452 86 12 Cuts at: 6937 7283 1972 Size: 346 1977 Fragments arranged by size: 2151 1977 1452 495 385 367 346 86 17 12 Bsu36I CC'TnA_GG Cuts at: 3775 3775 Size: 7288 BtgI C'CryG_G Cuts at: 513 1065 2044 2149 3567 513 Size: 552 979 105 1418 4234 Fragments arranged by size: 4234 1418 979 552 105 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 4122 487 Size: 683 56 2896 3653 Fragments arranged by size: 3653 2896 683 56 BtsI GCAGTG_nn' Cuts at: 2076 3128 4804 5598 5618 2076 Size: 1052 1676 794 20 3746 Fragments arranged by size: 3746 1676 1052 794 20 ClaI AT'CG_AT Cuts at: 1554 3897 1554 Size: 2343 4945 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 4146 4181 529 Size: 35 3582 35 3636 Fragments arranged by size: 3636 3582 35 35 DraI TTT'AAA Cuts at: 3553 4592 3553 Size: 1039 6249 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 6295 809 Size: 507 4979 1802 Fragments arranged by size: 4979 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2146 3798 4123 Size: 54 1425 50 275 18 316 1652 325 Cuts at: 4123 4469 4531 4735 5624 7253 8 Size: 346 62 204 889 1629 43 Fragments arranged by size: 1652 1629 1425 889 346 325 316 275 204 62 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 4735 1537 Size: 275 2923 4090 Fragments arranged by size: 4090 2923 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2454 5218 1353 Size: 1101 2764 3423 Fragments arranged by size: 3423 2764 1101 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 3940 5875 6249 6395 387 Size: 1064 138 2351 1935 374 146 1280 Fragments arranged by size: 2351 1935 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 3160 3850 3160 Size: 690 6598 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2183 2310 2562 2759 3039 3228 3639 Size: 393 551 127 252 197 280 189 411 Cuts at: 3639 3874 3893 4256 4281 4433 5351 5948 6735 Size: 235 19 363 25 152 918 597 787 Cuts at: 6735 7003 1239 Size: 268 1524 Fragments arranged by size: 1524 918 787 597 551 411 393 363 280 268 252 235 197 189 152 127 25 19 EcoO109I rG'GnC_Cy Cuts at: 2040 3115 3116 3137 2040 Size: 1075 1 21 6191 Fragments arranged by size: 6191 1075 21 1 EcoRI G'AATT_C Cuts at: 4597 4975 4597 Size: 378 6910 EcoRV GAT'ATC Cuts at: 2205 2205 Size: 7288 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 3013 3013 Size: 7288 FspI TGC'GCA Cuts at: 5794 5794 Size: 7288 HaeII r_GCGC'y Cuts at: 1678 4435 6435 1678 Size: 2757 2000 2531 Fragments arranged by size: 2757 2531 2000 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2230 2262 2359 2391 3529 Size: 32 135 32 658 32 97 32 1138 Cuts at: 3529 3561 5929 5961 6003 6035 1373 Size: 32 2368 32 42 32 2626 Fragments arranged by size: 2626 2368 1138 658 135 97 42 32 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 4632 4878 669 Size: 988 281 2694 246 3079 Fragments arranged by size: 3079 2694 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 7288 HpaI GTT'AAC Cuts at: 4878 4878 Size: 7288 KpnI G_GTAC'C Cuts at: 4613 4613 Size: 7288 MfeI C'AATT_G Cuts at: 2718 4887 2718 Size: 2169 5119 MluI A'CGCG_T Cuts at: 7164 7164 Size: 7288 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 3369 6402 6586 1305 Size: 2064 3033 184 2007 Fragments arranged by size: 3033 2064 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 3800 4471 4533 10 Size: 54 1425 343 1968 671 62 2765 Fragments arranged by size: 2765 1968 1425 671 343 62 54 MslI CAynn'nnrTG Cuts at: 518 1166 3396 3572 3578 4386 5246 5605 5764 Size: 648 2230 176 6 808 860 359 159 Cuts at: 5764 518 Size: 2042 Fragments arranged by size: 2230 2042 860 808 648 359 176 159 6 MspA1I CmG'CkG Cuts at: 1606 2046 2065 3920 3975 4129 4407 4704 5381 Size: 440 19 1855 55 154 278 297 677 Cuts at: 5381 6529 6774 1606 Size: 1148 245 2120 Fragments arranged by size: 2120 1855 1148 677 440 297 278 245 154 55 19 NcoI C'CATG_G Cuts at: 513 1065 3567 513 Size: 552 2502 4234 Fragments arranged by size: 4234 2502 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 7288 NheI G'CTAG_C Cuts at: 1052 1052 Size: 7288 NotI GC'GGCC_GC Cuts at: 4735 4735 Size: 7288 NspI r_CATG'y Cuts at: 1132 1229 3577 4084 4646 6191 1132 Size: 97 2348 507 562 1545 2229 Fragments arranged by size: 2348 2229 1545 562 507 97 PasI CC'CwG_GG Cuts at: 1348 3119 1348 Size: 1771 5517 PciI A'CATG_T Cuts at: 6187 6187 Size: 7288 PflMI CCAn_nnn'nTGG Cuts at: 1108 4286 4516 1108 Size: 3178 230 3880 Fragments arranged by size: 3880 3178 230 PfoI T'CCnGG_A Cuts at: 4285 4285 Size: 7288 PmeI GTTT'AAAC Cuts at: 4592 4592 Size: 7288 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 5357 5389 6870 6902 5357 Size: 32 1481 32 5743 Fragments arranged by size: 5743 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 7288 PsiI TTA'TAA Cuts at: 2703 4858 2703 Size: 2155 5133 PspOMI G'GGCC_C Cuts at: 2040 3115 2040 Size: 1075 6213 PstI C_TGCA'G Cuts at: 830 4640 830 Size: 3810 3478 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 7288 PvuII CAG'CTG Cuts at: 2065 3920 4129 2065 Size: 1855 209 5224 Fragments arranged by size: 5224 1855 209 SacI G_AGCT'C Cuts at: 3162 3852 3162 Size: 690 6598 SacII CC_GC'GG Cuts at: 2047 2047 Size: 7288 SalI G'TCGA_C Cuts at: 1936 4630 1936 Size: 2694 4594 SapI GCTCTTCn'nnn_ Cuts at: 2454 2454 Size: 7288 SbfI CC_TGCA'GG Cuts at: 4640 4640 Size: 7288 ScaI AGT'ACT Cuts at: 5536 7248 5536 Size: 1712 5576 SexAI A'CCwGG_T Cuts at: 2092 2092 Size: 7288 SfcI C'TryA_G Cuts at: 826 1045 2525 3040 3821 4636 5771 6452 6643 Size: 219 1480 515 781 815 1135 681 191 Cuts at: 6643 826 Size: 1471 Fragments arranged by size: 1480 1471 1135 815 781 681 515 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 7288 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 7288 SmaI CCC'GGG Cuts at: 1872 4615 1872 Size: 2743 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 3628 5392 6293 6555 820 Size: 197 926 1685 1764 901 262 1553 Fragments arranged by size: 1764 1685 1553 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 5079 493 Size: 4586 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 7288 SphI G_CATG'C Cuts at: 1132 3577 4646 1132 Size: 2445 1069 3774 Fragments arranged by size: 3774 2445 1069 SspI AAT'ATT Cuts at: 5 52 2644 5212 5 Size: 47 2592 2568 2081 Fragments arranged by size: 2592 2568 2081 47 StuI AGG'CCT Cuts at: 1517 2113 1517 Size: 596 6692 StyI C'CwwG_G Cuts at: 513 1065 2108 3394 3567 4727 513 Size: 552 1043 1286 173 1160 3074 Fragments arranged by size: 3074 1286 1160 1043 552 173 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3677 5315 5332 5485 5670 6115 325 Size: 1255 2097 1638 17 153 185 445 1498 Fragments arranged by size: 2097 1638 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 3731 4077 5534 Size: 274 80 33 51 1443 1754 346 1457 Cuts at: 5534 7246 96 Size: 1712 138 Fragments arranged by size: 1754 1712 1457 1443 346 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2181 2213 2559 2591 7248 7280 2181 Size: 32 346 32 4657 32 2189 Fragments arranged by size: 4657 2189 346 32 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 7288 XbaI T'CTAG_A Cuts at: 4624 4624 Size: 7288 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2207 3486 2207 Size: 1279 6009 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 7288 XmaI C'CCGG_G Cuts at: 1870 4613 1870 Size: 2743 4545 XmnI GAAnn'nnTTC Cuts at: 3387 5417 3387 Size: 2030 5258 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 5096 276 Size: 53 83 186 936 3562 2468 Fragments arranged by size: 3562 2468 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AfeI AflII AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI AvrII BamHI BanI BanII BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI EcoRV FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PshAI PsiI PspOMI PstI PvuI PvuII SacI SacII SalI SapI SbfI ScaI SexAI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AscI BaeI BbeI BfrBI BsiWI BstXI BstZ17I DraIII EcoNI FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PmlI PpuMI PspXI PsrI RsrII SanDI SfiI SfoI SrfI SwaI