(Linear) (Six-base) MAPSORT of: KIEE9627.seq Check: 513 from: 1 to: 2583 KIEE9627.orf With 182 enzymes: SgfI * July 3, 2012 19:03 .. AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 0 305 557 754 1034 1634 1888 2251 2428 Size: 305 252 197 280 600 254 363 177 Cuts at: 2428 2583 Size: 155 Fragments arranged by size: 600 363 305 280 254 252 197 177 155 AfeI AGC'GCT Cuts at: 0 2428 2583 Size: 2428 155 AflII C'TTAA_G Cuts at: 0 1623 2583 Size: 1623 960 AhdI GACnn_n'nnGTC Cuts at: 0 368 2583 Size: 368 2215 AleI CACnn'nnGTG Cuts at: 0 1391 2583 Size: 1391 1192 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 0 832 864 2583 Size: 832 32 1719 Fragments arranged by size: 1719 832 32 AlwNI CAG_nnn'CTG Cuts at: 0 1135 2010 2583 Size: 1135 875 573 Fragments arranged by size: 1135 875 573 ApaI G_GGCC'C Cuts at: 0 39 1114 2583 Size: 39 1075 1469 Fragments arranged by size: 1469 1075 39 ApaLI G'TGCA_C Cuts at: 0 1451 2583 Size: 1451 1132 ApoI r'AATT_y Cuts at: 0 669 880 1364 2583 Size: 669 211 484 1219 Fragments arranged by size: 1219 669 484 211 AseI AT'TA_AT Cuts at: 0 1459 2583 Size: 1459 1124 AvaI C'yCGr_G Cuts at: 0 14 2583 Size: 14 2569 AvrII C'CTAG_G Cuts at: 0 103 2583 Size: 103 2480 BanII G_rGCy'C Cuts at: 0 15 39 70 1114 1157 1847 2024 2583 Size: 15 24 31 1044 43 690 177 559 Fragments arranged by size: 1044 690 559 177 43 31 24 15 BbsI GAAGACnn'nnnn_ Cuts at: 0 435 2268 2583 Size: 435 1833 315 Fragments arranged by size: 1833 435 315 BbvCI CC'TCA_GC Cuts at: 0 110 2583 Size: 110 2473 BglII A'GATC_T Cuts at: 0 462 2583 Size: 462 2121 Bme1580I G_kGCm'C Cuts at: 0 39 1114 1455 2583 Size: 39 1075 341 1128 Fragments arranged by size: 1128 1075 341 39 BmrI ACTGGGnnnn_n' Cuts at: 0 14 369 978 1329 2583 Size: 14 355 609 351 1254 Fragments arranged by size: 1254 609 355 351 14 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 0 1861 2583 Size: 1861 722 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 178 1223 1869 2276 2583 Size: 178 1045 646 407 307 Fragments arranged by size: 1045 646 407 307 178 Bpu10I CC'TnA_GC Cuts at: 0 110 1151 2583 Size: 110 1041 1432 Fragments arranged by size: 1432 1041 110 BsaBI GATnn'nnATC Cuts at: 0 580 1412 2583 Size: 580 832 1171 Fragments arranged by size: 1171 832 580 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 0 176 206 208 238 554 584 2583 Size: 176 30 2 30 316 30 1999 Fragments arranged by size: 1999 316 176 30 30 30 2 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 0 251 1853 2583 Size: 251 1602 730 Fragments arranged by size: 1602 730 251 BseYI C'CCAG_C Cuts at: 0 1915 2080 2583 Size: 1915 165 503 Fragments arranged by size: 1915 503 165 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 0 780 2583 Size: 780 1803 BsiHKAI G_wGCw'C Cuts at: 0 991 1157 1455 1847 2583 Size: 991 166 298 392 736 Fragments arranged by size: 991 736 392 298 166 BsmBI CGTCTCn'nnnn_ Cuts at: 0 2108 2583 Size: 2108 475 Bsp1286I G_dGCh'C Cuts at: 0 15 39 70 991 1114 1157 1455 1847 Size: 15 24 31 921 123 43 298 392 Cuts at: 1847 2024 2583 Size: 177 559 Fragments arranged by size: 921 559 392 298 177 123 43 31 24 15 BspHI T'CATG_A Cuts at: 0 258 665 2583 Size: 258 407 1918 Fragments arranged by size: 1918 407 258 BsrDI GCAATG_nn' Cuts at: 0 1796 2073 2583 Size: 1796 277 510 Fragments arranged by size: 1796 510 277 BsrGI T'GTAC_A Cuts at: 0 1726 2583 Size: 1726 857 BssSI C'ACGA_G Cuts at: 0 1873 2583 Size: 1873 710 BstBI TT'CG_AA Cuts at: 0 822 2583 Size: 822 1761 BstEII G'GTnAC_C Cuts at: 0 2304 2583 Size: 2304 279 BstYI r'GATC_y Cuts at: 0 462 2583 Size: 462 2121 Bsu36I CC'TnA_GG Cuts at: 0 1770 2583 Size: 1770 813 BtgI C'CryG_G Cuts at: 0 39 144 1562 2583 Size: 39 105 1418 1021 Fragments arranged by size: 1418 1021 105 39 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 0 2117 2583 Size: 2117 466 BtsI GCAGTG_nn' Cuts at: 0 71 1123 2583 Size: 71 1052 1460 Fragments arranged by size: 1460 1052 71 ClaI AT'CG_AT Cuts at: 0 1892 2583 Size: 1892 691 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 0 2141 2176 2583 Size: 2141 35 407 Fragments arranged by size: 2141 407 35 DraI TTT'AAA Cuts at: 0 1548 2583 Size: 1548 1035 EaeI y'GGCC_r Cuts at: 0 141 1793 2118 2464 2526 2583 Size: 141 1652 325 346 62 57 Fragments arranged by size: 1652 346 325 141 62 57 EarI CTCTTCn'nnn_ Cuts at: 0 449 2583 Size: 449 2134 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 0 1935 2583 Size: 1935 648 EcoICRI GAG'CTC Cuts at: 0 1155 1845 2583 Size: 1155 690 738 Fragments arranged by size: 1155 738 690 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 0 178 305 557 754 1034 1223 1634 1869 Size: 178 127 252 197 280 189 411 235 Cuts at: 1869 1888 2251 2276 2428 2583 Size: 19 363 25 152 155 Fragments arranged by size: 411 363 280 252 235 197 189 178 155 152 127 25 19 EcoO109I rG'GnC_Cy Cuts at: 0 35 1110 1111 1132 2583 Size: 35 1075 1 21 1451 Fragments arranged by size: 1451 1075 35 21 1 EcoRV GAT'ATC Cuts at: 0 200 2583 Size: 200 2383 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 0 1008 2583 Size: 1008 1575 HaeII r_GCGC'y Cuts at: 0 2430 2583 Size: 2430 153 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 0 225 257 354 386 1524 1556 2583 Size: 225 32 97 32 1138 32 1027 Fragments arranged by size: 1138 1027 225 97 32 32 32 MfeI C'AATT_G Cuts at: 0 713 2583 Size: 713 1870 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 0 1364 2583 Size: 1364 1219 MscI TGG'CCA Cuts at: 0 1795 2466 2528 2583 Size: 1795 671 62 55 Fragments arranged by size: 1795 671 62 55 MslI CAynn'nnrTG Cuts at: 0 1391 1567 1573 2381 2583 Size: 1391 176 6 808 202 Fragments arranged by size: 1391 808 202 176 6 MspA1I CmG'CkG Cuts at: 0 41 60 1915 1970 2124 2402 2583 Size: 41 19 1855 55 154 278 181 Fragments arranged by size: 1855 278 181 154 55 41 19 NcoI C'CATG_G Cuts at: 0 1562 2583 Size: 1562 1021 NspI r_CATG'y Cuts at: 0 1572 2079 2583 Size: 1572 507 504 Fragments arranged by size: 1572 507 504 PasI CC'CwG_GG Cuts at: 0 1114 2583 Size: 1114 1469 PflMI CCAn_nnn'nTGG Cuts at: 0 2281 2511 2583 Size: 2281 230 72 Fragments arranged by size: 2281 230 72 PfoI T'CCnGG_A Cuts at: 0 2280 2583 Size: 2280 303 PsiI TTA'TAA Cuts at: 0 698 2583 Size: 698 1885 PspOMI G'GGCC_C Cuts at: 0 35 1110 2583 Size: 35 1075 1473 Fragments arranged by size: 1473 1075 35 PvuII CAG'CTG Cuts at: 0 60 1915 2124 2583 Size: 60 1855 209 459 Fragments arranged by size: 1855 459 209 60 SacI G_AGCT'C Cuts at: 0 1157 1847 2583 Size: 1157 690 736 Fragments arranged by size: 1157 736 690 SacII CC_GC'GG Cuts at: 0 42 2583 Size: 42 2541 SapI GCTCTTCn'nnn_ Cuts at: 0 449 2583 Size: 449 2134 SexAI A'CCwGG_T Cuts at: 0 87 2583 Size: 87 2496 SfcI C'TryA_G Cuts at: 0 520 1035 1816 2583 Size: 520 515 781 767 Fragments arranged by size: 781 767 520 515 SmlI C'TyrA_G Cuts at: 0 1623 2583 Size: 1623 960 SphI G_CATG'C Cuts at: 0 1572 2583 Size: 1572 1011 SspI AAT'ATT Cuts at: 0 639 2583 Size: 639 1944 StuI AGG'CCT Cuts at: 0 108 2583 Size: 108 2475 StyI C'CwwG_G Cuts at: 0 103 1389 1562 2583 Size: 103 1286 173 1021 Fragments arranged by size: 1286 1021 173 103 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 0 1672 2583 Size: 1672 911 TatI w'GTAC_w Cuts at: 0 1726 2072 2583 Size: 1726 346 511 Fragments arranged by size: 1726 511 346 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 0 176 208 554 586 2583 Size: 176 32 346 32 1997 Fragments arranged by size: 1997 346 176 32 32 XcmI CCAnnnn_n'nnnnTGG Cuts at: 0 202 1481 2583 Size: 202 1279 1102 Fragments arranged by size: 1279 1102 202 XmnI GAAnn'nnTTC Cuts at: 0 1382 2583 Size: 1382 1201 Enzymes that do cut: AcuI AfeI AflII AhdI AleI AloI AlwNI ApaI ApaLI ApoI AseI AvaI AvrII BanII BbsI BbvCI BglII Bme1580I BmrI BplI BpmI Bpu10I BsaBI BsaXI BseRI BseYI BsgI BsiHKAI BsmBI Bsp1286I BspHI BsrDI BsrGI BssSI BstBI BstEII BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI EaeI EarI EciI EcoICRI Eco57MI EcoO109I EcoRV FalI HaeII Hin4I MfeI MmeI MscI MslI MspA1I NcoI NspI PasI PflMI PfoI PsiI PspOMI PvuII SacI SacII SapI SexAI SfcI SmlI SphI SspI StuI StyI TaqII TatI TstI XcmI XmnI Enzymes that do not cut: AarI AatII AccI Acc65I AclI AflIII AgeI AlfI AscI AsiSI BaeI BamHI BanI BbeI BcgI BciVI BclI BfrBI BglI BlpI BmgBI BmtI BpuEI BsaI BsaAI BsaHI BsaWI BsiEI BsiWI BsmI BspEI BspMI BsrBI BsrFI BssHII BstAPI BstXI BstZ17I DraIII DrdI EagI EcoNI EcoRI FseI FspI FspAI HincII HindIII HpaI KasI KpnI MluI NaeI NarI NdeI NgoMIV NheI NotI NruI NsiI PacI PciI PmeI PmlI PpiI PpuMI PshAI PspXI PsrI PstI PvuI RsrII SalI SanDI SbfI ScaI SfiI SfoI SgfI SgrAI SmaI SnaBI SpeI SrfI SwaI Tth111I XbaI XhoI XmaI ZraI