################################## # NO CUT ENZYMES ( 110 enzymes) # ################################## AatII AccIII AclI AcyI AflII AgeI AhdI Alw44I AocI Aor51HI ApaLI AscI AseI AsnI AspEI AspHI AspI AviII BalI BamHI BbeI BbrPI BbvCI BciVI BfrI BglI BlpI Bpu1102I BsaHI BsaWI BseAI BsiEI BsiHKAI BsmI BspEI BspHI BspLU11I BsrFI BssHII BssSI BstBI BstEII BstPI BstXI Bsu36I CelII Cfr10I CpoI DrdI EagI Eam1105I EclXI Eco47III Eco52I Eco81I EcoO65I EcoRI EcoT22I FseI FspI HaeII Hin1I HindIII HpaI KasI KspI MfeI MluNI MroI MscI MunI NaeI NarI NgoMIV NheI NotI NruI NsiI NspV PacI PaeR7I PflFI PinAI PmaCI PmeI PmlI PshBI Psp1406I PvuI RcaI RsrII SacI SacII SalI SapI SbfI SfiI SfoI SfuI SgrAI SmaI SpeI SphI Sse8387I SwaI Tth111I XbaI XhoI XmaCI XmaI ################################### # ONE CUT ENZYMES ( 45 enzymes) # ################################### Acc65I 2275 GGTACC G^GTACC AccII 1234 CGCG CG^CG AlwNI 1872 CAGATACTG CAG[ACGT][ACGT][ACGT]^CTG ApaI 29 GGGCCC GGGCC^C Asp718I 2275 GGTACC G^GTACC AvaI 386 CTCGGG C^[CT]CG[GA]G AvrII 175 CCTAGG C^CTAGG BclI 1094 TGATCA T^GATCA BglII 1111 AGATCT A^GATCT BlnI 175 CCTAGG C^CTAGG BsaAI 2371 TACGTA [CT]AC^GT[GA] BseRI 1670 CTCCTC GAGGAG(10/8) BsiWI 2373 CGTACG C^GTACG BsmBI 828 GAGACG CGTCTC(1/5) BsmFI 1307 GGGAC GGGAC(10/14) BsoBI 386 CTCGGG C^[CT]CG[GA]G BspDI 652 ATCGAT AT^CGAT BsrBI 551 CCGCTC CCGCTC(-3/-3) BsrDI 2504 GCAATG GCAATG(2/0) Bst1107I 948 GTATAC GTA^TAC BstUI 1234 CGCG CG^CG BstZ17I 948 GTATAC GTA^TAC CfoI 928 GCGC GCG^C ClaI 652 ATCGAT AT^CGAT DraIII 1634 CACAATGTG CAC[ACGT][ACGT][ACGT]^GTG EaeI 903 CGGCCA [CT]^GGCC[GA] EcoRV 2444 GATATC GAT^ATC FbaI 1094 TGATCA T^GATCA HgaI 1232 GACGC GACGC(5/10) HhaI 928 GCGC GCG^C HinP1I 928 GCGC G^CGC KpnI 2275 GGTACC GGTAC^C MluI 1233 ACGCGT A^CGCGT MvnI 1234 CGCG CG^CG NdeI 2231 CATATG CA^TATG NspI 1310 ACATGC [GA]CATG^[CT] PpuMI 18 AGGACCC [GA]G^G[AT]CC[CT] PshAI 166 GACAAGTGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC PstI 2327 CTGCAG CTGCA^G ScaI 414 AGTACT AGT^ACT SexAI 2423 ACCTGGT A^CC[AT]GGT SmlI 1525 CTCAAG C^T[CT][GA]AG SnaBI 2371 TACGTA TAC^GTA StuI 862 AGGCCT AGG^CCT XcmI 86 CCATTAAACTGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG ################################### # TWO CUT ENZYMES ( 29 enzymes) # ################################### AcsI 376 AAATTT [GA]^AATT[CT] 772 AAATTC AflIII 1233 ACGCGT A^C[GA][CT]GT 1602 ACGTGT ApoI 376 AAATTT [GA]^AATT[CT] 772 AAATTC Asp700I 1320 GAAACAATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC 1404 GAAGGGTTTC BanII 29 GGGCCC G[GA]GC[CT]^C 1507 GAGCCC BbsI 56 GAAGAC GAAGAC(2/6) 1575 GAAGAC BcgI 654 CGATGTTTCTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10) 2065 CGAGAAGCCTGC BcnI 1570 CCGGG CC^[GC]GG 1941 CCGGG BpmI 554 CTCCAG CTGGAG(16/14) 1255 CTCCAG BpuAI 56 GAAGAC GAAGAC(2/6) 1575 GAAGAC BsaBI 757 GATTCTCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC 1449 GATGCCCATC BsaI 239 GAGACC GGTCTC(1/5) 1899 GAGACC Bsp1407I 2380 TGTACA T^GTACA 2538 TGTACA BsrGI 2380 TGTACA T^GTACA 2538 TGTACA BstAPI 399 GCACCTACTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC 1108 GCAAGATCTGC DdeI 437 CTGAG C^T[ACGT]AG 1436 CTAAG DraI 220 TTTAAA TTT^AAA 1198 TTTAAA DraII 18 AGGACCC [GA]G^G[ACGT]CC[CT] 28 GGGGCCC EarI 953 CTCTTC CTCTTC(1/4) 2127 GAAGAG EcoO109I 18 AGGACCC [GA]G^G[ACGT]CC[CT] 28 GGGGCCC FauI 22 CCCGC CCCGC(4/6) 969 CCCGC HincII 199 GTTGAC GT[CT]^[GA]AC 1968 GTCAAC HindII 199 GTTGAC GT[CT]^[GA]AC 1968 GTCAAC Ksp632I 953 CTCTTC CTCTTC(1/4) 2127 GAAGAG MamI 757 GATTCTCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC 1449 GATGCCCATC NciI 1570 CCGGG CC^[GC]GG 1941 CCGGG PleI 202 GACTC GAGTC(4/5) 810 GACTC SspBI 2380 TGTACA T^GTACA 2538 TGTACA XmnI 1320 GAAACAATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC 1404 GAAGGGTTTC ######################################### # 3 OR MORE CUT ENZYMES ( 74 enzymes) # ######################################### AccI AciI AfaI AluI AlwI AvaII BanI BbvI BfaI BmyI BsaJI BsgI BsiYI BslI BsmAI Bsp1286I BspMI BsrI BssKI BstF5I BstNI BstYI Cac8I Cfr13I DpnII DsaI EcoNI EcoRII EcoT14I Fnu4HI FokI HaeIII HapII HinfI HpaII HphI ItaI MaeI MaeII MaeIII MboI MboII MflI MnlI MseI MslI MspA1I MspI MvaI MwoI NcoI NdeII NlaIII NlaIV PflMI PvuII RsaI Sau3AI Sau96I ScrFI SfaNI SfcI SspI StyI TaqI TfiI Tru9I TseI Tsp45I Tsp509I TspRI TthHB8I Van91I XhoII