(Circular) (Six-base) MAPSORT of: pFN21AE9626.seq Check: 459 from: 1 to: 7285 pFN21AE9626 7285 bp With 182 enzymes: SgfI * July 6, 2012 17:34 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 3820 3820 Size: 7285 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 5095 278 Size: 53 83 186 936 3559 2468 Fragments arranged by size: 3559 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2142 2251 2954 4628 1937 Size: 205 109 703 1674 4594 Fragments arranged by size: 4594 1674 703 205 109 Acc65I G'GTAC_C Cuts at: 4280 4606 4280 Size: 326 6959 AclI AA'CG_TT Cuts at: 1563 5412 5785 1563 Size: 3849 373 3063 Fragments arranged by size: 3849 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2945 2987 4328 4503 5348 6732 7000 1632 Size: 1313 42 1341 175 845 1384 268 1917 Fragments arranged by size: 1917 1384 1341 1313 845 268 175 42 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 7088 AflIII A'CryG_T Cuts at: 1203 3238 3607 6184 7161 1203 Size: 2035 369 2577 977 1327 Fragments arranged by size: 2577 2035 1327 977 369 AgeI A'CCGG_T Cuts at: 6116 6116 Size: 7285 AhdI GACnn_n'nnGTC Cuts at: 6014 6014 Size: 7285 AleI CACnn'nnGTG Cuts at: 1166 2534 1166 Size: 1368 5917 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 7285 AlwNI CAG_nnn'CTG Cuts at: 3882 5028 6600 3882 Size: 1146 1572 4567 Fragments arranged by size: 4567 1572 1146 ApaI G_GGCC'C Cuts at: 2038 2038 Size: 7285 ApaLI G'TGCA_C Cuts at: 5342 6498 5342 Size: 1156 6129 ApoI r'AATT_y Cuts at: 1493 2381 2777 4594 4820 4972 7081 1493 Size: 888 396 1817 226 152 2109 1697 Fragments arranged by size: 2109 1817 1697 888 396 226 152 AseI AT'TA_AT Cuts at: 160 1032 5839 160 Size: 872 4807 1606 Fragments arranged by size: 4807 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 7285 AvaI C'yCGr_G Cuts at: 1870 1943 2391 4610 1870 Size: 73 448 2219 4545 Fragments arranged by size: 4545 2219 448 73 AvrII C'CTAG_G Cuts at: 2180 2180 Size: 7285 BamHI G'GATC_C Cuts at: 4615 4982 4615 Size: 367 6918 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2053 2075 2403 4280 4606 Size: 325 217 627 266 22 328 1877 326 Cuts at: 4606 6061 618 Size: 1455 1842 Fragments arranged by size: 1877 1842 1455 627 328 326 325 266 217 22 BanII G_rGCy'C Cuts at: 1391 2038 3516 4604 1391 Size: 647 1478 1088 4072 Fragments arranged by size: 4072 1478 1088 647 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2068 3587 928 Size: 971 169 1519 4626 Fragments arranged by size: 4626 1519 971 169 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 2648 2682 4059 4093 5476 5510 1903 Size: 34 711 34 1377 34 1383 34 3678 Fragments arranged by size: 3678 1383 1377 711 34 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 5177 6387 5177 Size: 1210 6075 BclI T'GATC_A Cuts at: 1548 3099 1548 Size: 1551 5734 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 5896 136 Size: 107 122 71 631 605 4224 1525 Fragments arranged by size: 4224 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 3116 7280 3116 Size: 4164 3121 BlpI GC'TnA_GC Cuts at: 4702 4702 Size: 7285 Bme1580I G_kGCm'C Cuts at: 1790 2038 2058 5346 6502 1790 Size: 248 20 3288 1156 2573 Fragments arranged by size: 3288 2573 1156 248 20 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 7285 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 5016 5974 442 Size: 416 53 83 399 24 3599 958 1753 Fragments arranged by size: 3599 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 7285 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2544 3245 5945 1239 Size: 1305 701 2700 2579 Fragments arranged by size: 2700 2579 1305 701 Bpu10I CC'TnA_GC Cuts at: 2442 2442 Size: 7285 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3515 5410 6275 6573 3515 Size: 1895 865 298 4227 Fragments arranged by size: 4227 1895 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2238 3898 5948 882 Size: 617 739 1660 2050 2219 Fragments arranged by size: 2219 2050 1660 739 617 BsaAI yAC'GTr Cuts at: 493 1204 4378 5076 493 Size: 711 3174 698 2702 Fragments arranged by size: 3174 2702 711 698 BsaBI GATnn'nnATC Cuts at: 2766 3458 7279 2766 Size: 692 3821 2772 Fragments arranged by size: 3821 2772 692 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 5092 5474 6109 275 Size: 53 83 186 936 3559 382 635 1451 Fragments arranged by size: 3559 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 5718 6116 6390 6537 6948 5718 Size: 398 274 147 411 6055 Fragments arranged by size: 6055 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 3243 3273 3783 3813 3920 Size: 30 165 30 1840 30 510 30 107 Cuts at: 3920 3950 1178 Size: 30 4513 Fragments arranged by size: 4513 1840 510 165 107 30 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 3666 1186 Size: 2480 4805 BseYI C'CCAG_C Cuts at: 2030 2037 4198 6488 6984 2030 Size: 7 2161 2290 496 2331 Fragments arranged by size: 2331 2290 2161 496 7 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 3105 3412 4392 1163 Size: 573 1369 307 980 4056 Fragments arranged by size: 4056 1369 980 573 307 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 4735 5496 6524 1540 Size: 275 186 2734 761 1028 2301 Fragments arranged by size: 2734 2301 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 5346 5431 6502 5346 Size: 85 1071 6129 Fragments arranged by size: 6129 1071 85 BsiWI C'GTAC_G Cuts at: 4378 4378 Size: 7285 BsmI GAATG_Cn' Cuts at: 4796 4889 4796 Size: 93 7192 BsmBI CGTCTCn'nnnn_ Cuts at: 2827 2827 Size: 7285 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2038 2058 3516 4604 5346 5431 6502 Size: 399 248 20 1458 1088 742 85 1071 Cuts at: 6502 1391 Size: 2174 Fragments arranged by size: 2174 1458 1088 1071 742 399 248 85 20 BspEI T'CCGG_A Cuts at: 6948 6948 Size: 7285 BspHI T'CATG_A Cuts at: 5174 6904 5174 Size: 1730 5555 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3030 3820 4030 4414 4640 844 Size: 2186 790 210 384 226 3489 Fragments arranged by size: 3489 2186 790 384 226 210 BsrBI CCG'CTC Cuts at: 1394 1823 2558 5172 1394 Size: 429 735 2614 3507 Fragments arranged by size: 3507 2614 735 429 BsrDI GCAATG_nn' Cuts at: 65 1248 4516 5780 5954 65 Size: 1183 3268 1264 174 1396 Fragments arranged by size: 3268 1396 1264 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 5929 6116 1700 Size: 110 4119 187 2869 Fragments arranged by size: 4119 2869 187 110 BsrGI T'GTAC_A Cuts at: 96 4385 4543 96 Size: 4289 158 2838 Fragments arranged by size: 4289 2838 158 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 7285 BssSI C'ACGA_G Cuts at: 5345 6357 5345 Size: 1012 6273 BstAPI GCAn_nnn'nTGC Cuts at: 794 2410 3119 794 Size: 1616 709 4960 Fragments arranged by size: 4960 1616 709 BstBI TT'CG_AA Cuts at: 6089 6089 Size: 7285 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 7285 BstYI r'GATC_y Cuts at: 1972 3116 3178 4519 4615 4982 5367 5384 6836 Size: 1144 62 1341 96 367 385 17 1452 Cuts at: 6836 6922 6934 7280 1972 Size: 86 12 346 1977 Fragments arranged by size: 1977 1452 1341 1144 385 367 346 96 86 62 17 12 BstZ17I GTA'TAC Cuts at: 2955 2955 Size: 7285 BtgI C'CryG_G Cuts at: 513 1065 2004 2079 3301 3939 513 Size: 552 939 75 1222 638 3859 Fragments arranged by size: 3859 1222 939 638 552 75 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 6546 Fragments arranged by size: 6546 683 56 BtsI GCAGTG_nn' Cuts at: 2670 3395 4801 5595 5615 2670 Size: 725 1406 794 20 4340 Fragments arranged by size: 4340 1406 794 725 20 ClaI AT'CG_AT Cuts at: 1554 2658 1554 Size: 1104 6181 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 7250 DraI TTT'AAA Cuts at: 2227 3205 4589 2227 Size: 978 1384 4923 Fragments arranged by size: 4923 1384 978 DraIII CAC_nnn'GTG Cuts at: 3644 3644 Size: 7285 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 6292 809 Size: 507 4976 1802 Fragments arranged by size: 4976 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2908 4732 5621 Size: 54 1425 50 275 18 1078 1824 889 Cuts at: 5621 7250 8 Size: 1629 43 Fragments arranged by size: 1824 1629 1425 1078 889 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 4732 1537 Size: 275 2920 4090 Fragments arranged by size: 4090 2920 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2964 4127 5215 1353 Size: 1611 1163 1088 3423 Fragments arranged by size: 3423 1611 1163 1088 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 5872 6246 6392 387 Size: 1064 138 4283 374 146 1280 Fragments arranged by size: 4283 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2544 2945 2987 3245 4328 4503 5348 Size: 393 912 401 42 258 1083 175 845 Cuts at: 5348 5945 6732 7000 1239 Size: 597 787 268 1524 Fragments arranged by size: 1524 1083 912 845 787 597 401 393 268 258 175 42 EcoNI CCTnn'n_nnAGG Cuts at: 2150 2343 2583 2150 Size: 193 240 6852 Fragments arranged by size: 6852 240 193 EcoO109I rG'GnC_Cy Cuts at: 2024 2034 2024 Size: 10 7275 EcoRI G'AATT_C Cuts at: 4594 4972 4594 Size: 378 6907 EcoRV GAT'ATC Cuts at: 4451 4451 Size: 7285 FspI TGC'GCA Cuts at: 5791 5791 Size: 7285 HaeII r_GCGC'y Cuts at: 1678 6432 1678 Size: 4754 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2749 2781 3333 3365 3783 Size: 32 135 32 1177 32 552 32 418 Cuts at: 3783 3815 3903 3920 3935 3952 5926 5958 6000 Size: 32 88 17 15 17 1974 32 42 Cuts at: 6000 6032 1373 Size: 32 2626 Fragments arranged by size: 2626 1974 1177 552 418 135 88 42 32 32 32 32 32 32 32 17 17 15 HincII GTy'rAC Cuts at: 669 1657 1938 2206 3975 4629 4875 669 Size: 988 281 268 1769 654 246 3079 Fragments arranged by size: 3079 1769 988 654 281 268 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 7285 HpaI GTT'AAC Cuts at: 4875 4875 Size: 7285 KpnI G_GTAC'C Cuts at: 4284 4610 4284 Size: 326 6959 MfeI C'AATT_G Cuts at: 4884 4884 Size: 7285 MluI A'CGCG_T Cuts at: 3238 7161 3238 Size: 3923 3362 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 4159 6399 6583 1305 Size: 2854 2240 184 2007 Fragments arranged by size: 2854 2240 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 5463 Fragments arranged by size: 5463 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2534 4236 4302 4476 5243 5602 5761 Size: 648 1368 1702 66 174 767 359 159 Cuts at: 5761 518 Size: 2042 Fragments arranged by size: 2042 1702 1368 767 648 359 174 159 66 MspA1I CmG'CkG Cuts at: 1606 2030 2965 3427 4202 4348 4701 5378 6526 Size: 424 935 462 775 146 353 677 1148 Cuts at: 6526 6771 1606 Size: 245 2120 Fragments arranged by size: 2120 1148 935 775 677 462 424 353 245 146 NcoI C'CATG_G Cuts at: 513 1065 2004 2079 3301 3939 513 Size: 552 939 75 1222 638 3859 Fragments arranged by size: 3859 1222 939 638 552 75 NdeI CA'TA_TG Cuts at: 387 4237 387 Size: 3850 3435 NheI G'CTAG_C Cuts at: 1052 1052 Size: 7285 NotI GC'GGCC_GC Cuts at: 4732 4732 Size: 7285 NspI r_CATG'y Cuts at: 1132 1229 3319 4643 6188 1132 Size: 97 2090 1324 1545 2229 Fragments arranged by size: 2229 2090 1545 1324 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 7285 PciI A'CATG_T Cuts at: 6184 6184 Size: 7285 PflMI CCAn_nnn'nTGG Cuts at: 1108 2581 2917 3815 3931 1108 Size: 1473 336 898 116 4462 Fragments arranged by size: 4462 1473 898 336 116 PfoI T'CCnGG_A Cuts at: 3924 3945 3924 Size: 21 7264 PmeI GTTT'AAAC Cuts at: 4589 4589 Size: 7285 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3503 3535 5354 5386 6867 6899 3503 Size: 32 1819 32 1481 32 3889 Fragments arranged by size: 3889 1819 1481 32 32 32 PpuMI rG'GwC_Cy Cuts at: 2024 2024 Size: 7285 PshAI GACnn'nnGTC Cuts at: 1613 2175 1613 Size: 562 6723 PsiI TTA'TAA Cuts at: 3969 4855 3969 Size: 886 6399 PspOMI G'GGCC_C Cuts at: 2034 2034 Size: 7285 PstI C_TGCA'G Cuts at: 830 4336 4637 830 Size: 3506 301 3478 Fragments arranged by size: 3506 3478 301 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 7285 PvuII CAG'CTG Cuts at: 2965 3427 4202 4348 2965 Size: 462 775 146 5902 Fragments arranged by size: 5902 775 462 146 SalI G'TCGA_C Cuts at: 1936 4627 1936 Size: 2691 4594 SbfI CC_TGCA'GG Cuts at: 4637 4637 Size: 7285 ScaI AGT'ACT Cuts at: 2421 5533 7245 2421 Size: 3112 1712 2461 Fragments arranged by size: 3112 2461 1712 SexAI A'CCwGG_T Cuts at: 4428 4428 Size: 7285 SfcI C'TryA_G Cuts at: 826 1045 2310 3551 4258 4332 4633 5768 6449 Size: 219 1265 1241 707 74 301 1135 681 Cuts at: 6449 6640 826 Size: 191 1471 Fragments arranged by size: 1471 1265 1241 1135 707 681 301 219 191 74 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 7285 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 7285 SmaI CCC'GGG Cuts at: 1872 4612 1872 Size: 2740 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 3530 5389 6290 6552 820 Size: 197 926 1587 1859 901 262 1553 Fragments arranged by size: 1859 1587 1553 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 4378 5076 493 Size: 3885 698 2702 Fragments arranged by size: 3885 2702 698 SpeI A'CTAG_T Cuts at: 152 152 Size: 7285 SphI G_CATG'C Cuts at: 1132 4643 1132 Size: 3511 3774 SspI AAT'ATT Cuts at: 5 52 2734 3043 3405 3736 5209 5 Size: 47 2682 309 362 331 1473 2081 Fragments arranged by size: 2682 2081 1473 362 331 309 47 StuI AGG'CCT Cuts at: 1517 2869 1517 Size: 1352 5933 StyI C'CwwG_G Cuts at: 513 1065 2004 2079 2180 3301 3939 4536 4724 Size: 552 939 75 101 1121 638 597 188 Cuts at: 4724 513 Size: 3074 Fragments arranged by size: 3074 1121 939 638 597 552 188 101 75 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3885 4248 5312 5329 5482 5667 6112 Size: 1255 2305 363 1064 17 153 185 445 Cuts at: 6112 325 Size: 1498 Fragments arranged by size: 2305 1498 1255 1064 445 363 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2419 4385 4543 Size: 274 80 33 51 1443 442 1966 158 Cuts at: 4543 4575 5531 7243 96 Size: 32 956 1712 138 Fragments arranged by size: 1966 1712 1443 956 442 274 158 138 80 51 33 32 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2275 2307 2830 2862 3243 3275 7245 7277 2275 Size: 32 523 32 381 32 3970 32 2283 Fragments arranged by size: 3970 2283 523 381 32 32 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 7285 XbaI T'CTAG_A Cuts at: 4621 4621 Size: 7285 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2098 2098 Size: 7285 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 7285 XmaI C'CCGG_G Cuts at: 1870 4610 1870 Size: 2740 4545 XmnI GAAnn'nnTTC Cuts at: 3329 3413 5414 3329 Size: 84 2001 5200 Fragments arranged by size: 5200 2001 84 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 5093 276 Size: 53 83 186 936 3559 2468 Fragments arranged by size: 3559 2468 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI AvrII BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsiWI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BstZ17I BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI Eco57MI EcoNI EcoO109I EcoRI EcoRV FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI PvuII SalI SbfI ScaI SexAI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI AloI AscI BaeI BbeI BbvCI BfrBI BplI BstXI Bsu36I EcoICRI FalI FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PmlI PspXI PsrI RsrII SacI SacII SanDI SapI SfiI SfoI SrfI SwaI