(Linear) (Six-base) MAPSORT of: KIEE9626.seq Check: 9809 from: 1 to: 2580 KIEE9626.orf With 182 enzymes: SgfI * July 3, 2012 19:03 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 0 1815 2580 Size: 1815 765 AccI GT'mk_AC Cuts at: 0 137 246 949 2580 Size: 137 109 703 1631 Fragments arranged by size: 1631 703 137 109 Acc65I G'GTAC_C Cuts at: 0 2275 2580 Size: 2275 305 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 0 940 982 2323 2498 2580 Size: 940 42 1341 175 82 Fragments arranged by size: 1341 940 175 82 42 AflIII A'CryG_T Cuts at: 0 1233 1602 2580 Size: 1233 369 978 Fragments arranged by size: 1233 978 369 AleI CACnn'nnGTG Cuts at: 0 529 2580 Size: 529 2051 AlwNI CAG_nnn'CTG Cuts at: 0 1877 2580 Size: 1877 703 ApaI G_GGCC'C Cuts at: 0 33 2580 Size: 33 2547 ApoI r'AATT_y Cuts at: 0 376 772 2580 Size: 376 396 1808 Fragments arranged by size: 1808 396 376 AvaI C'yCGr_G Cuts at: 0 386 2580 Size: 386 2194 AvrII C'CTAG_G Cuts at: 0 175 2580 Size: 175 2405 BanI G'GyrC_C Cuts at: 0 48 70 398 2275 2580 Size: 48 22 328 1877 305 Fragments arranged by size: 1877 328 305 48 22 BanII G_rGCy'C Cuts at: 0 33 1511 2580 Size: 33 1478 1069 Fragments arranged by size: 1478 1069 33 BbsI GAAGACnn'nnnn_ Cuts at: 0 63 1582 2580 Size: 63 1519 998 Fragments arranged by size: 1519 998 63 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 0 643 677 2054 2088 2580 Size: 643 34 1377 34 492 Fragments arranged by size: 1377 643 492 34 34 BclI T'GATC_A Cuts at: 0 1094 2580 Size: 1094 1486 BglII A'GATC_T Cuts at: 0 1111 2580 Size: 1111 1469 Bme1580I G_kGCm'C Cuts at: 0 33 53 2580 Size: 33 20 2527 Fragments arranged by size: 2527 33 20 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 539 1240 2580 Size: 539 701 1340 Fragments arranged by size: 1340 701 539 Bpu10I CC'TnA_GC Cuts at: 0 437 2580 Size: 437 2143 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 1510 2580 Size: 1510 1070 BsaI GGTCTCn'nnnn_ Cuts at: 0 233 1893 2580 Size: 233 1660 687 Fragments arranged by size: 1660 687 233 BsaAI yAC'GTr Cuts at: 0 2373 2580 Size: 2373 207 BsaBI GATnn'nnATC Cuts at: 0 761 1453 2580 Size: 761 692 1127 Fragments arranged by size: 1127 761 692 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 0 1238 1268 1778 1808 1915 1945 2580 Size: 1238 30 510 30 107 30 635 Fragments arranged by size: 1238 635 510 107 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 0 1661 2580 Size: 1661 919 BseYI C'CCAG_C Cuts at: 0 25 32 2193 2580 Size: 25 7 2161 387 Fragments arranged by size: 2161 387 25 7 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 0 1100 1407 2387 2580 Size: 1100 307 980 193 Fragments arranged by size: 1100 980 307 193 BsiWI C'GTAC_G Cuts at: 0 2373 2580 Size: 2373 207 BsmBI CGTCTCn'nnnn_ Cuts at: 0 822 2580 Size: 822 1758 Bsp1286I G_dGCh'C Cuts at: 0 33 53 1511 2580 Size: 33 20 1458 1069 Fragments arranged by size: 1458 1069 33 20 BspMI ACCTGCnnnn'nnnn_ Cuts at: 0 1025 1815 2025 2409 2580 Size: 1025 790 210 384 171 Fragments arranged by size: 1025 790 384 210 171 BsrBI CCG'CTC Cuts at: 0 553 2580 Size: 553 2027 BsrDI GCAATG_nn' Cuts at: 0 2511 2580 Size: 2511 69 BsrGI T'GTAC_A Cuts at: 0 2380 2538 2580 Size: 2380 158 42 Fragments arranged by size: 2380 158 42 BstAPI GCAn_nnn'nTGC Cuts at: 0 405 1114 2580 Size: 405 709 1466 Fragments arranged by size: 1466 709 405 BstYI r'GATC_y Cuts at: 0 1111 1173 2514 2580 Size: 1111 62 1341 66 Fragments arranged by size: 1341 1111 66 62 BstZ17I GTA'TAC Cuts at: 0 950 2580 Size: 950 1630 BtgI C'CryG_G Cuts at: 0 74 1296 1934 2580 Size: 74 1222 638 646 Fragments arranged by size: 1222 646 638 74 BtsI GCAGTG_nn' Cuts at: 0 665 1390 2580 Size: 665 725 1190 Fragments arranged by size: 1190 725 665 ClaI AT'CG_AT Cuts at: 0 653 2580 Size: 653 1927 DraI TTT'AAA Cuts at: 0 222 1200 2580 Size: 222 978 1380 Fragments arranged by size: 1380 978 222 DraIII CAC_nnn'GTG Cuts at: 0 1639 2580 Size: 1639 941 EaeI y'GGCC_r Cuts at: 0 903 2580 Size: 903 1677 EarI CTCTTCn'nnn_ Cuts at: 0 959 2122 2580 Size: 959 1163 458 Fragments arranged by size: 1163 959 458 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 0 539 940 982 1240 2323 2498 2580 Size: 539 401 42 258 1083 175 82 Fragments arranged by size: 1083 539 401 258 175 82 42 EcoNI CCTnn'n_nnAGG Cuts at: 0 145 338 578 2580 Size: 145 193 240 2002 Fragments arranged by size: 2002 240 193 145 EcoO109I rG'GnC_Cy Cuts at: 0 19 29 2580 Size: 19 10 2551 Fragments arranged by size: 2551 19 10 EcoRV GAT'ATC Cuts at: 0 2446 2580 Size: 2446 134 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 0 744 776 1328 1360 1778 1810 1898 1915 Size: 744 32 552 32 418 32 88 17 Cuts at: 1915 1930 1947 2580 Size: 15 17 633 Fragments arranged by size: 744 633 552 418 88 32 32 32 17 17 15 HincII GTy'rAC Cuts at: 0 201 1970 2580 Size: 201 1769 610 Fragments arranged by size: 1769 610 201 KpnI G_GTAC'C Cuts at: 0 2279 2580 Size: 2279 301 MluI A'CGCG_T Cuts at: 0 1233 2580 Size: 1233 1347 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 0 2154 2580 Size: 2154 426 MslI CAynn'nnrTG Cuts at: 0 529 2231 2297 2471 2580 Size: 529 1702 66 174 109 Fragments arranged by size: 1702 529 174 109 66 MspA1I CmG'CkG Cuts at: 0 25 960 1422 2197 2343 2580 Size: 25 935 462 775 146 237 Fragments arranged by size: 935 775 462 237 146 25 NcoI C'CATG_G Cuts at: 0 74 1296 1934 2580 Size: 74 1222 638 646 Fragments arranged by size: 1222 646 638 74 NdeI CA'TA_TG Cuts at: 0 2232 2580 Size: 2232 348 NspI r_CATG'y Cuts at: 0 1314 2580 Size: 1314 1266 PflMI CCAn_nnn'nTGG Cuts at: 0 576 912 1810 1926 2580 Size: 576 336 898 116 654 Fragments arranged by size: 898 654 576 336 116 PfoI T'CCnGG_A Cuts at: 0 1919 1940 2580 Size: 1919 21 640 Fragments arranged by size: 1919 640 21 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 0 1498 1530 2580 Size: 1498 32 1050 Fragments arranged by size: 1498 1050 32 PpuMI rG'GwC_Cy Cuts at: 0 19 2580 Size: 19 2561 PshAI GACnn'nnGTC Cuts at: 0 170 2580 Size: 170 2410 PsiI TTA'TAA Cuts at: 0 1964 2580 Size: 1964 616 PspOMI G'GGCC_C Cuts at: 0 29 2580 Size: 29 2551 PstI C_TGCA'G Cuts at: 0 2331 2580 Size: 2331 249 PvuII CAG'CTG Cuts at: 0 960 1422 2197 2343 2580 Size: 960 462 775 146 237 Fragments arranged by size: 960 775 462 237 146 ScaI AGT'ACT Cuts at: 0 416 2580 Size: 416 2164 SexAI A'CCwGG_T Cuts at: 0 2423 2580 Size: 2423 157 SfcI C'TryA_G Cuts at: 0 305 1546 2253 2327 2580 Size: 305 1241 707 74 253 Fragments arranged by size: 1241 707 305 253 74 SmlI C'TyrA_G Cuts at: 0 1525 2580 Size: 1525 1055 SnaBI TAC'GTA Cuts at: 0 2373 2580 Size: 2373 207 SspI AAT'ATT Cuts at: 0 729 1038 1400 1731 2580 Size: 729 309 362 331 849 Fragments arranged by size: 849 729 362 331 309 StuI AGG'CCT Cuts at: 0 864 2580 Size: 864 1716 StyI C'CwwG_G Cuts at: 0 74 175 1296 1934 2531 2580 Size: 74 101 1121 638 597 49 Fragments arranged by size: 1121 638 597 101 74 49 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 0 1880 2243 2580 Size: 1880 363 337 Fragments arranged by size: 1880 363 337 TatI w'GTAC_w Cuts at: 0 414 2380 2538 2570 2580 Size: 414 1966 158 32 10 Fragments arranged by size: 1966 414 158 32 10 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 0 270 302 825 857 1238 1270 2580 Size: 270 32 523 32 381 32 1310 Fragments arranged by size: 1310 523 381 270 32 32 32 XcmI CCAnnnn_n'nnnnTGG Cuts at: 0 93 2580 Size: 93 2487 XmnI GAAnn'nnTTC Cuts at: 0 1324 1408 2580 Size: 1324 84 1172 Fragments arranged by size: 1324 1172 84 Enzymes that do cut: AarI AccI Acc65I AcuI AflIII AleI AlwNI ApaI ApoI AvaI AvrII BanI BanII BbsI BcgI BclI BglII Bme1580I BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaXI BseRI BseYI BsgI BsiWI BsmBI Bsp1286I BspMI BsrBI BsrDI BsrGI BstAPI BstYI BstZ17I BtgI BtsI ClaI DraI DraIII EaeI EarI Eco57MI EcoNI EcoO109I EcoRV Hin4I HincII KpnI MluI MmeI MslI MspA1I NcoI NdeI NspI PflMI PfoI PpiI PpuMI PshAI PsiI PspOMI PstI PvuII ScaI SexAI SfcI SmlI SnaBI SspI StuI StyI TaqII TatI TstI XcmI XmnI Enzymes that do not cut: AatII AclI AfeI AflII AgeI AhdI AlfI AloI ApaLI AscI AseI AsiSI BaeI BamHI BbeI BbvCI BciVI BfrBI BglI BlpI BmgBI BmrI BmtI BplI BsaHI BsaWI BsiEI BsiHKAI BsmI BspEI BspHI BsrFI BssHII BssSI BstBI BstEII BstXI Bsu36I BtgZI CspCI DrdI EagI EciI EcoICRI EcoRI FalI FseI FspI FspAI HaeII HindIII HpaI KasI MfeI MscI NaeI NarI NgoMIV NheI NotI NruI NsiI PacI PasI PciI PmeI PmlI PspXI PsrI PvuI RsrII SacI SacII SalI SanDI SapI SbfI SfiI SfoI SgfI SgrAI SmaI SpeI SphI SrfI SwaI Tth111I XbaI XhoI XmaI ZraI