################################## # NO CUT ENZYMES ( 133 enzymes) # ################################## AatII AccIII AclI AcsI AflII AflIII AgeI AhdI Alw44I AlwI AlwNI AocI Aor51HI ApaI ApaLI ApoI AscI AseI AsnI Asp700I AspEI AspHI AspI AvaI AviII BamHI BbrPI BbsI BbvCI BcgI BclI BcnI BfrI BglII BpuAI BsaBI BsaWI BseAI BsiHKAI BsiWI BsmBI BsmI BsoBI Bsp1407I BspDI BspEI BspHI BspLU11I BsrGI BssHII BssSI Bst1107I BstBI BstEII BstPI BstYI BstZ17I Bsu36I ClaI DraI DrdI EagI Eam1105I EclXI Eco47III Eco52I Eco81I EcoNI EcoO65I EcoRI EcoRV EcoT22I FbaI FseI FspI HincII HindII HindIII HpaI MamI MfeI MflI MluI MroI MseI MunI NciI NcoI NdeI NheI NotI NruI NsiI NspV PacI PaeR7I PflFI PinAI PleI PmaCI PmeI PmlI PshAI PshBI Psp1406I RcaI SacI SalI SapI SbfI ScaI SexAI SfiI SfuI SgrAI SmaI SmlI SnaBI SpeI Sse8387I SspBI SspI StuI SwaI Tru9I Tsp509I Tth111I XbaI XhoI XhoII XmaCI XmaI XmnI ################################### # ONE CUT ENZYMES ( 39 enzymes) # ################################### Acc65I 358 GGTACC G^GTACC Asp718I 358 GGTACC G^GTACC AvrII 429 CCTAGG C^CTAGG BalI 653 TGGCCA TGG^CCA BanII 241 GAGCCC G[GA]GC[CT]^C BciVI 851 GTATCC GTATCC(6/5) BfaI 430 CTAG C^TAG BlnI 429 CCTAGG C^CTAGG BlpI 546 GCTCAGC GC^T[ACGT]AGC BmyI 241 GAGCCC G[AGT]GC[ACT]^C BpmI 238 CTGGAG CTGGAG(16/14) Bpu1102I 546 GCTCAGC GC^T[ACGT]AGC BsaAI 216 CACGTA [CT]AC^GT[GA] BseRI 175 GAGGAG GAGGAG(10/8) BsgI 152 CTGCAC GTGCAG(16/14) Bsp1286I 241 GAGCCC G[AGT]GC[ACT]^C BsrBI 544 CCGCTC CCGCTC(-3/-3) BsrDI 230 GCAATG GCAATG(2/0) BstAPI 758 GCATGCACTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC CelII 546 GCTCAGC GC^T[ACGT]AGC CpoI 540 CGGACCG CG^G[AT]CCG DraIII 323 CACTCCGTG CAC[ACGT][ACGT][ACGT]^GTG EarI 34 CTCTTC CTCTTC(1/4) EcoT14I 429 CCTAGG C^C[AT][AT]GG KpnI 358 GGTACC GGTAC^C Ksp632I 34 CTCTTC CTCTTC(1/4) MaeI 430 CTAG C^TAG MluNI 653 TGGCCA TGG^CCA MscI 653 TGGCCA TGG^CCA PflMI 160 CCAAGTAATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG PpuMI 75 GGGACCC [GA]G^G[AT]CC[CT] PvuI 461 CGATCG CGAT^CG PvuII 807 CAGCTG CAG^CTG RsrII 540 CGGACCG CG^G[AT]CCG StyI 429 CCTAGG C^C[AT][AT]GG TaqI 455 TCGA T^CGA TthHB8I 455 TCGA T^CGA Van91I 160 CCAAGTAATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG XcmI 160 CCAAGTAATGGGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG ################################### # TWO CUT ENZYMES ( 28 enzymes) # ################################### AccI 382 GTCTAC GT^[AC][GT]AC 616 GTCTAC AfaI 219 GTAC GT^AC 359 GTAC BbeI 349 GGCGCC GGCGC^C 769 GGCGCC BglI 127 GCCTCCCAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC 428 GCCTAGGCGGC BsaI 4 GAGACC GGTCTC(1/5) 370 GAGACC BsiEI 201 CGACCG CG[GA][CT]^CG 461 CGATCG BspMI 7 ACCTGC ACCTGC(4/8) 361 ACCTGC DdeI 398 CTGAG C^T[ACGT]AG 547 CTCAG DraII 75 GGGACCC [GA]G^G[ACGT]CC[CT] 647 GGGGCCT EcoO109I 75 GGGACCC [GA]G^G[ACGT]CC[CT] 647 GGGGCCT HgaI 420 GCGTC GACGC(5/10) 614 GCGTC HinfI 118 GATTC G^A[ACGT]TC 821 GATTC HphI 499 GGTGA GGTGA(8/7) 835 GGTGA KasI 349 GGCGCC G^GCGCC 769 GGCGCC KspI 288 CCGCGG CCGC^GG 353 CCGCGG MaeIII 329 GTGAC ^GT[ACGT]AC 836 GTGAC MboII 35 TCTTC GAAGA(8/7) 302 TCTTC NaeI 440 GCCGGC GCC^GGC 551 GCCGGC NarI 349 GGCGCC GG^CGCC 769 GGCGCC NgoMIV 440 GCCGGC G^CCGGC 551 GCCGGC NlaIII 590 CATG CATG^ 759 CATG NspI 589 GCATGC [GA]CATG^[CT] 758 GCATGC RsaI 219 GTAC GT^AC 359 GTAC SacII 288 CCGCGG CCGC^GG 353 CCGCGG SfoI 349 GGCGCC GGC^GCC 769 GGCGCC SphI 589 GCATGC GCATG^C 758 GCATGC TfiI 118 GATTC G^A[AT]TC 821 GATTC Tsp45I 329 GTGAC ^GT[GC]AC 836 GTGAC ######################################### # 3 OR MORE CUT ENZYMES ( 58 enzymes) # ######################################### AccII AciI AcyI AluI AvaII BanI BbvI BsaHI BsaJI BsiYI BslI BsmAI BsmFI BsrFI BsrI BssKI BstF5I BstNI BstUI BstXI Cac8I CfoI Cfr10I Cfr13I DpnII DsaI EaeI EcoRII FauI Fnu4HI FokI HaeII HaeIII HapII HhaI Hin1I HinP1I HpaII ItaI MaeII MboI MnlI MslI MspA1I MspI MvaI MvnI MwoI NdeII NlaIV PstI Sau3AI Sau96I ScrFI SfaNI SfcI TseI TspRI