(Circular) (Six-base) MAPSORT of: pFN21AE3541.seq Check: 6316 from: 1 to: 5590 pFN21AE3541 5590 bp With 182 enzymes: SgfI * July 6, 2012 17:34 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3400 278 Size: 53 83 186 936 1864 2468 Fragments arranged by size: 2468 1864 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2388 2622 2933 1937 Size: 451 234 311 4594 Fragments arranged by size: 4594 451 311 234 Acc65I G'GTAC_C Cuts at: 2363 2911 2363 Size: 548 5042 AclI AA'CG_TT Cuts at: 1563 3717 4090 1563 Size: 2154 373 3063 Fragments arranged by size: 3063 2154 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 3653 5037 5305 1632 Size: 2021 1384 268 1917 Fragments arranged by size: 2021 1917 1384 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5393 AflIII A'CryG_T Cuts at: 1203 4489 5466 1203 Size: 3286 977 1327 Fragments arranged by size: 3286 1327 977 AgeI A'CCGG_T Cuts at: 4421 4421 Size: 5590 AhdI GACnn_n'nnGTC Cuts at: 4319 4319 Size: 5590 AleI CACnn'nnGTG Cuts at: 1166 2531 2702 1166 Size: 1365 171 4054 Fragments arranged by size: 4054 1365 171 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 5590 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2306 2338 2306 Size: 32 5558 AlwNI CAG_nnn'CTG Cuts at: 3333 4905 3333 Size: 1572 4018 ApaLI G'TGCA_C Cuts at: 3647 4803 3647 Size: 1156 4434 ApoI r'AATT_y Cuts at: 1493 2899 3125 3277 5386 1493 Size: 1406 226 152 2109 1697 Fragments arranged by size: 2109 1697 1406 226 152 AseI AT'TA_AT Cuts at: 160 1032 4144 160 Size: 872 3112 1606 Fragments arranged by size: 3112 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5590 AvaI C'yCGr_G Cuts at: 1870 1943 2915 1870 Size: 73 972 4545 Fragments arranged by size: 4545 972 73 AvrII C'CTAG_G Cuts at: 2434 2434 Size: 5590 BamHI G'GATC_C Cuts at: 2920 3287 2920 Size: 367 5223 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2354 2363 2774 2794 2911 Size: 325 217 627 567 9 411 20 117 Cuts at: 2911 4366 618 Size: 1455 1842 Fragments arranged by size: 1842 1455 627 567 411 325 217 117 20 9 BanII G_rGCy'C Cuts at: 1391 2250 2909 1391 Size: 859 659 4072 Fragments arranged by size: 4072 859 659 BbeI G_GCGC'C Cuts at: 2358 2778 2358 Size: 420 5170 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 4619 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 3781 3815 1903 Size: 34 1844 34 3678 Fragments arranged by size: 3678 1844 34 34 BciVI GTATCCnnnnn_n' Cuts at: 2867 3482 4692 2867 Size: 615 1210 3765 Fragments arranged by size: 3765 1210 615 BclI T'GATC_A Cuts at: 1548 1548 Size: 5590 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2138 2439 4201 Size: 107 122 71 631 605 466 301 1762 Cuts at: 4201 136 Size: 1525 Fragments arranged by size: 1762 1525 631 605 466 301 122 107 71 BglII A'GATC_T Cuts at: 5585 5585 Size: 5590 BlpI GC'TnA_GC Cuts at: 2552 3007 2552 Size: 455 5135 Bme1580I G_kGCm'C Cuts at: 1790 3651 4807 1790 Size: 1861 1156 2573 Fragments arranged by size: 2573 1861 1156 BmgBI CAC'GTC Cuts at: 1318 2587 1318 Size: 1269 4321 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3321 4279 442 Size: 416 53 83 399 24 1904 958 1753 Fragments arranged by size: 1904 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5590 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2264 4250 1239 Size: 1025 1986 2579 Fragments arranged by size: 2579 1986 1025 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3715 4580 4878 3715 Size: 865 298 4427 Fragments arranged by size: 4427 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2003 2369 4253 882 Size: 617 504 366 1884 2219 Fragments arranged by size: 2219 1884 617 504 366 BsaAI yAC'GTr Cuts at: 493 1204 2223 3381 493 Size: 711 1019 1158 2702 Fragments arranged by size: 2702 1158 1019 711 BsaBI GATnn'nnATC Cuts at: 5584 5584 Size: 5590 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2355 2425 2775 3397 Size: 53 83 186 936 822 70 350 622 Cuts at: 3397 3779 4414 275 Size: 382 635 1451 Fragments arranged by size: 1451 936 822 635 622 382 350 186 83 70 53 BsaWI w'CCGG_w Cuts at: 4023 4421 4695 4842 5253 4023 Size: 398 274 147 411 4360 Fragments arranged by size: 4360 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 5365 Fragments arranged by size: 5365 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2195 1186 Size: 1009 4581 BseYI C'CCAG_C Cuts at: 2146 2668 2867 4793 5289 2146 Size: 522 199 1926 496 2447 Fragments arranged by size: 2447 1926 522 496 199 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2142 1163 Size: 573 406 4611 Fragments arranged by size: 4611 573 406 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2209 2469 3040 3801 4829 1540 Size: 275 186 208 260 571 761 1028 2301 Fragments arranged by size: 2301 1028 761 571 275 260 208 186 BsiHKAI G_wGCw'C Cuts at: 3651 3736 4807 3651 Size: 85 1071 4434 Fragments arranged by size: 4434 1071 85 BsmI GAATG_Cn' Cuts at: 3101 3194 3101 Size: 93 5497 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2250 2909 3651 3736 4807 1391 Size: 399 460 659 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 659 460 399 85 BspEI T'CCGG_A Cuts at: 5253 5253 Size: 5590 BspHI T'CATG_A Cuts at: 3479 5209 3479 Size: 1730 3860 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2021 2375 2945 844 Size: 1177 354 570 3489 Fragments arranged by size: 3489 1177 570 354 BsrBI CCG'CTC Cuts at: 1394 1823 2551 3477 1394 Size: 429 728 926 3507 Fragments arranged by size: 3507 926 728 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2242 4085 4259 65 Size: 1183 994 1843 174 1396 Fragments arranged by size: 1843 1396 1183 994 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2018 2445 2556 4234 4421 1700 Size: 110 208 427 111 1678 187 2869 Fragments arranged by size: 2869 1678 427 208 187 111 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5590 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5590 BssSI C'ACGA_G Cuts at: 3650 4662 3650 Size: 1012 4578 BstAPI GCAn_nnn'nTGC Cuts at: 794 2769 794 Size: 1975 3615 BstBI TT'CG_AA Cuts at: 4394 4394 Size: 5590 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5590 BstXI CCAn_nnnn'nTGG Cuts at: 2704 2837 2867 2704 Size: 133 30 5427 Fragments arranged by size: 5427 133 30 BstYI r'GATC_y Cuts at: 1972 2920 3287 3672 3689 5141 5227 5239 5585 Size: 948 367 385 17 1452 86 12 346 Cuts at: 5585 1972 Size: 1977 Fragments arranged by size: 1977 1452 948 385 367 346 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2004 2287 2293 2358 2419 2875 513 Size: 552 939 283 6 65 61 456 3228 Fragments arranged by size: 3228 939 552 456 283 65 61 6 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 4851 Fragments arranged by size: 4851 683 56 BtsI GCAGTG_nn' Cuts at: 2159 2639 2767 3106 3900 3920 2159 Size: 480 128 339 794 20 3829 Fragments arranged by size: 3829 794 480 339 128 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5590 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5555 DraI TTT'AAA Cuts at: 2894 2894 Size: 5590 DraIII CAC_nnn'GTG Cuts at: 2333 2333 Size: 5590 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4597 809 Size: 507 3281 1802 Fragments arranged by size: 3281 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2290 2658 2712 Size: 54 1425 50 275 18 460 368 54 Cuts at: 2712 3037 3926 5555 8 Size: 325 889 1629 43 Fragments arranged by size: 1629 1425 889 460 368 325 275 54 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3037 1537 Size: 275 1225 4090 Fragments arranged by size: 4090 1225 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2045 3520 1353 Size: 692 1475 3423 Fragments arranged by size: 3423 1475 692 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2489 4177 4551 4697 387 Size: 1064 138 900 1688 374 146 1280 Fragments arranged by size: 1688 1280 1064 900 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2264 3653 4250 5037 5305 1239 Size: 393 632 1389 597 787 268 1524 Fragments arranged by size: 1524 1389 787 632 597 393 268 EcoO109I rG'GnC_Cy Cuts at: 2081 2653 2081 Size: 572 5018 EcoRI G'AATT_C Cuts at: 2899 3277 2899 Size: 378 5212 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2395 2395 Size: 5590 FspI TGC'GCA Cuts at: 4096 4096 Size: 5590 HaeII r_GCGC'y Cuts at: 1678 2358 2606 2691 2778 4737 1678 Size: 680 248 85 87 1959 2531 Fragments arranged by size: 2531 1959 680 248 87 85 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 4231 4263 4305 4337 1373 Size: 32 135 32 2659 32 42 32 2626 Fragments arranged by size: 2659 2626 135 42 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2934 3180 669 Size: 988 281 996 246 3079 Fragments arranged by size: 3079 996 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 5590 HpaI GTT'AAC Cuts at: 3180 3180 Size: 5590 KasI G'GCGC_C Cuts at: 2354 2774 2354 Size: 420 5170 KpnI G_GTAC'C Cuts at: 2367 2915 2367 Size: 548 5042 MfeI C'AATT_G Cuts at: 3189 3189 Size: 5590 MluI A'CGCG_T Cuts at: 5466 5466 Size: 5590 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 4704 4888 1305 Size: 3399 184 2007 Fragments arranged by size: 3399 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2660 10 Size: 54 1425 343 828 2940 Fragments arranged by size: 2940 1425 828 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2309 2531 2702 3548 3907 4066 518 Size: 648 1143 222 171 846 359 159 2042 Fragments arranged by size: 2042 1143 846 648 359 222 171 159 MspA1I CmG'CkG Cuts at: 1606 2032 2190 2295 2360 2808 2814 3006 3683 Size: 426 158 105 65 448 6 192 677 Cuts at: 3683 4831 5076 1606 Size: 1148 245 2120 Fragments arranged by size: 2120 1148 677 448 426 245 192 158 105 65 6 NaeI GCC'GGC Cuts at: 2447 2558 2447 Size: 111 5479 NarI GG'CG_CC Cuts at: 2355 2775 2355 Size: 420 5170 NcoI C'CATG_G Cuts at: 513 1065 2004 513 Size: 552 939 4099 Fragments arranged by size: 4099 939 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 5590 NgoMIV G'CCGG_C Cuts at: 2445 2556 2445 Size: 111 5479 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5590 NotI GC'GGCC_GC Cuts at: 3037 3037 Size: 5590 NspI r_CATG'y Cuts at: 1132 1229 2598 2767 2948 4493 1132 Size: 97 1369 169 181 1545 2229 Fragments arranged by size: 2229 1545 1369 181 169 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 5590 PciI A'CATG_T Cuts at: 4489 4489 Size: 5590 PflMI CCAn_nnn'nTGG Cuts at: 1108 2171 1108 Size: 1063 4527 PfoI T'CCnGG_A Cuts at: 2118 2829 2118 Size: 711 4879 PmeI GTTT'AAAC Cuts at: 2894 2894 Size: 5590 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3659 3691 5172 5204 3659 Size: 32 1481 32 4045 Fragments arranged by size: 4045 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2081 2081 Size: 5590 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5590 PsiI TTA'TAA Cuts at: 3160 3160 Size: 5590 PstI C_TGCA'G Cuts at: 830 2271 2322 2342 2813 2819 2942 830 Size: 1441 51 20 471 6 123 3478 Fragments arranged by size: 3478 1441 471 123 51 20 6 PvuI CG_AT'CG Cuts at: 2001 2469 2001 Size: 468 5122 PvuII CAG'CTG Cuts at: 2814 2814 Size: 5590 RsrII CG'GwC_CG Cuts at: 2546 2546 Size: 5590 SacII CC_GC'GG Cuts at: 2296 2361 2296 Size: 65 5525 SalI G'TCGA_C Cuts at: 1936 2932 1936 Size: 996 4594 SanDI GG'GwC_CC Cuts at: 2081 2081 Size: 5590 SbfI CC_TGCA'GG Cuts at: 2942 2942 Size: 5590 ScaI AGT'ACT Cuts at: 3838 5550 3838 Size: 1712 3878 SfcI C'TryA_G Cuts at: 826 1045 2267 2318 2338 2809 2815 2938 4073 Size: 219 1222 51 20 471 6 123 1135 Cuts at: 4073 4754 4945 826 Size: 681 191 1471 Fragments arranged by size: 1471 1222 1135 681 471 219 191 123 51 20 6 SfoI GGC'GCC Cuts at: 2356 2776 2356 Size: 420 5170 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5590 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5590 SmaI CCC'GGG Cuts at: 1872 2917 1872 Size: 1045 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 3694 4595 4857 820 Size: 197 926 1751 901 262 1553 Fragments arranged by size: 1751 1553 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 3381 493 Size: 2888 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5590 SphI G_CATG'C Cuts at: 1132 2598 2767 2948 1132 Size: 1466 169 181 3774 Fragments arranged by size: 3774 1466 181 169 SspI AAT'ATT Cuts at: 5 52 3514 5 Size: 47 3462 2081 Fragments arranged by size: 3462 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 5590 StyI C'CwwG_G Cuts at: 513 1065 2004 2434 3029 513 Size: 552 939 430 595 3074 Fragments arranged by size: 3074 939 595 552 430 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2163 2492 3617 3634 3787 3972 4417 Size: 1255 583 329 1125 17 153 185 445 Cuts at: 4417 325 Size: 1498 Fragments arranged by size: 1498 1255 1125 583 445 329 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 3836 5548 96 Size: 274 80 33 51 1443 1859 1712 138 Fragments arranged by size: 1859 1712 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2272 2304 2709 2741 5550 5582 2272 Size: 32 405 32 2809 32 2280 Fragments arranged by size: 2809 2280 405 32 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5590 XbaI T'CTAG_A Cuts at: 2926 2926 Size: 5590 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2172 2172 Size: 5590 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5590 XmaI C'CCGG_G Cuts at: 1870 2915 1870 Size: 1045 4545 XmnI GAAnn'nnTTC Cuts at: 3719 3719 Size: 5590 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3398 276 Size: 53 83 186 936 1864 2468 Fragments arranged by size: 2468 1864 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaLI ApoI AseI AsiSI AvaI AvrII BamHI BanI BanII BbeI BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI Eco57MI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PstI PvuI PvuII RsrII SacII SalI SanDI SbfI ScaI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI ApaI AscI BaeI BbvCI BfrBI BplI Bpu10I BsiWI BsmBI BstZ17I Bsu36I EcoICRI EcoNI EcoRV FseI FspAI NruI NsiI PacI PmlI PspOMI PspXI PsrI SacI SapI SexAI SfiI SrfI SwaI