Restriction map for
pFN21AE3541
(Linear) (Six-base) MAPSORT of: KIEE3541.seq Check: 9351 from: 1 to: 885
KIEE3541.orf
With 182 enzymes: SgfI *
July 3, 2012 18:09 ..
AccI GT'mk_AC
Cuts at: 0 383 617 885
Size: 383 234 268
Fragments arranged by size:
383 268 234
Acc65I G'GTAC_C
Cuts at: 0 358 885
Size: 358 527
AleI CACnn'nnGTG
Cuts at: 0 526 697 885
Size: 526 171 188
Fragments arranged by size:
526 188 171
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 301 333 885
Size: 301 32 552
Fragments arranged by size:
552 301 32
AvrII C'CTAG_G
Cuts at: 0 429 885
Size: 429 456
BanI G'GyrC_C
Cuts at: 0 349 358 769 789 885
Size: 349 9 411 20 96
Fragments arranged by size:
411 349 96 20 9
BanII G_rGCy'C
Cuts at: 0 245 885
Size: 245 640
BbeI G_GCGC'C
Cuts at: 0 353 773 885
Size: 353 420 112
Fragments arranged by size:
420 353 112
BciVI GTATCCnnnnn_n'
Cuts at: 0 862 885
Size: 862 23
BglI GCCn_nnn'nGGC
Cuts at: 0 133 434 885
Size: 133 301 451
Fragments arranged by size:
451 301 133
BlpI GC'TnA_GC
Cuts at: 0 547 885
Size: 547 338
BmgBI CAC'GTC
Cuts at: 0 582 885
Size: 582 303
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 259 885
Size: 259 626
BsaI GGTCTCn'nnnn_
Cuts at: 0 364 885
Size: 364 521
BsaAI yAC'GTr
Cuts at: 0 218 885
Size: 218 667
BsaHI Gr'CG_yC
Cuts at: 0 350 420 770 885
Size: 350 70 350 115
Fragments arranged by size:
350 350 115 70
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 190 885
Size: 190 695
BseYI C'CCAG_C
Cuts at: 0 141 663 862 885
Size: 141 522 199 23
Fragments arranged by size:
522 199 141 23
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 137 885
Size: 137 748
BsiEI CG_ry'CG
Cuts at: 0 204 464 885
Size: 204 260 421
Fragments arranged by size:
421 260 204
Bsp1286I G_dGCh'C
Cuts at: 0 245 885
Size: 245 640
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 16 370 885
Size: 16 354 515
Fragments arranged by size:
515 354 16
BsrBI CCG'CTC
Cuts at: 0 546 885
Size: 546 339
BsrDI GCAATG_nn'
Cuts at: 0 237 885
Size: 237 648
BsrFI r'CCGG_y
Cuts at: 0 13 440 551 885
Size: 13 427 111 334
Fragments arranged by size:
427 334 111 13
BstAPI GCAn_nnn'nTGC
Cuts at: 0 764 885
Size: 764 121
BstXI CCAn_nnnn'nTGG
Cuts at: 0 699 832 862 885
Size: 699 133 30 23
Fragments arranged by size:
699 133 30 23
BtgI C'CryG_G
Cuts at: 0 282 288 353 414 870 885
Size: 282 6 65 61 456 15
Fragments arranged by size:
456 282 65 61 15 6
BtsI GCAGTG_nn'
Cuts at: 0 154 634 762 885
Size: 154 480 128 123
Fragments arranged by size:
480 154 128 123
DraIII CAC_nnn'GTG
Cuts at: 0 328 885
Size: 328 557
EaeI y'GGCC_r
Cuts at: 0 285 653 707 885
Size: 285 368 54 178
Fragments arranged by size:
368 285 178 54
EarI CTCTTCn'nnn_
Cuts at: 0 40 885
Size: 40 845
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 484 885
Size: 484 401
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 259 885
Size: 259 626
EcoO109I rG'GnC_Cy
Cuts at: 0 76 648 885
Size: 76 572 237
Fragments arranged by size:
572 237 76
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 390 885
Size: 390 495
HaeII r_GCGC'y
Cuts at: 0 353 601 686 773 885
Size: 353 248 85 87 112
Fragments arranged by size:
353 248 112 87 85
KasI G'GCGC_C
Cuts at: 0 349 769 885
Size: 349 420 116
Fragments arranged by size:
420 349 116
KpnI G_GTAC'C
Cuts at: 0 362 885
Size: 362 523
MscI TGG'CCA
Cuts at: 0 655 885
Size: 655 230
MslI CAynn'nnrTG
Cuts at: 0 304 526 697 885
Size: 304 222 171 188
Fragments arranged by size:
304 222 188 171
MspA1I CmG'CkG
Cuts at: 0 27 185 290 355 803 809 885
Size: 27 158 105 65 448 6 76
Fragments arranged by size:
448 158 105 76 65 27 6
NaeI GCC'GGC
Cuts at: 0 442 553 885
Size: 442 111 332
Fragments arranged by size:
442 332 111
NarI GG'CG_CC
Cuts at: 0 350 770 885
Size: 350 420 115
Fragments arranged by size:
420 350 115
NgoMIV G'CCGG_C
Cuts at: 0 440 551 885
Size: 440 111 334
Fragments arranged by size:
440 334 111
NspI r_CATG'y
Cuts at: 0 593 762 885
Size: 593 169 123
Fragments arranged by size:
593 169 123
PflMI CCAn_nnn'nTGG
Cuts at: 0 166 885
Size: 166 719
PfoI T'CCnGG_A
Cuts at: 0 113 824 885
Size: 113 711 61
Fragments arranged by size:
711 113 61
PpuMI rG'GwC_Cy
Cuts at: 0 76 885
Size: 76 809
PstI C_TGCA'G
Cuts at: 0 266 317 337 808 814 885
Size: 266 51 20 471 6 71
Fragments arranged by size:
471 266 71 51 20 6
PvuI CG_AT'CG
Cuts at: 0 464 885
Size: 464 421
PvuII CAG'CTG
Cuts at: 0 809 885
Size: 809 76
RsrII CG'GwC_CG
Cuts at: 0 541 885
Size: 541 344
SacII CC_GC'GG
Cuts at: 0 291 356 885
Size: 291 65 529
Fragments arranged by size:
529 291 65
SanDI GG'GwC_CC
Cuts at: 0 76 885
Size: 76 809
SfcI C'TryA_G
Cuts at: 0 262 313 333 804 810 885
Size: 262 51 20 471 6 75
Fragments arranged by size:
471 262 75 51 20 6
SfoI GGC'GCC
Cuts at: 0 351 771 885
Size: 351 420 114
Fragments arranged by size:
420 351 114
SphI G_CATG'C
Cuts at: 0 593 762 885
Size: 593 169 123
Fragments arranged by size:
593 169 123
StyI C'CwwG_G
Cuts at: 0 429 885
Size: 429 456
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 158 487 885
Size: 158 329 398
Fragments arranged by size:
398 329 158
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 267 299 704 736 885
Size: 267 32 405 32 149
Fragments arranged by size:
405 267 149 32 32
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 167 885
Size: 167 718
Enzymes that do cut:
AccI Acc65I AleI AloI AvrII BanI BanII BbeI
BciVI BglI BlpI BmgBI BpmI BsaI BsaAI BsaHI
BseRI BseYI BsgI BsiEI Bsp1286I BspMI BsrBI BsrDI
BsrFI BstAPI BstXI BtgI BtsI DraIII EaeI EarI
EciI Eco57MI EcoO109I FalI HaeII KasI KpnI MscI
MslI MspA1I NaeI NarI NgoMIV NspI PflMI PfoI
PpuMI PstI PvuI PvuII RsrII SacII SanDI SfcI
SfoI SphI StyI TaqII TstI XcmI
Enzymes that do not cut:
AarI AatII AclI AcuI AfeI AflII AflIII AgeI
AhdI AlfI AlwNI ApaI ApaLI ApoI AscI AseI
AsiSI AvaI BaeI BamHI BbsI BbvCI BcgI BclI
BfrBI BglII Bme1580I BmrI BmtI BplI Bpu10I BpuEI
BsaBI BsaWI BsaXI BsiHKAI BsiWI BsmI BsmBI BspEI
BspHI BsrGI BssHII BssSI BstBI BstEII BstYI BstZ17I
Bsu36I BtgZI ClaI CspCI DraI DrdI EagI EcoICRI
EcoNI EcoRI EcoRV FseI FspI FspAI Hin4I HincII
HindIII HpaI MfeI MluI MmeI NcoI NdeI NheI
NotI NruI NsiI PacI PasI PciI PmeI PmlI
PpiI PshAI PsiI PspOMI PspXI PsrI SacI SalI
SapI SbfI ScaI SexAI SfiI SgfI SgrAI SmaI
SmlI SnaBI SpeI SrfI SspI StuI SwaI TatI
Tth111I XbaI XhoI XmaI XmnI ZraI