################################## # NO CUT ENZYMES ( 142 enzymes) # ################################## AatII Acc65I AccI AccIII AclI AcsI AcyI AflII AgeI AhdI AlwI AocI Aor51HI ApoI AscI AseI AsnI Asp700I Asp718I AspEI AviII AvrII BamHI BanI BbeI BbrPI BbvCI BcgI BciVI BfrI BglII BlnI BlpI BpmI Bpu1102I BsaAI BsaBI BsaHI BsaWI BseAI BsiEI BsiWI BsmI Bsp1407I BspDI BspEI BsrBI BsrDI BsrGI BssHII BssSI Bst1107I BstAPI BstBI BstEII BstPI BstXI BstYI BstZ17I Bsu36I CelII ClaI CpoI DraI DraIII EagI Eam1105I EclXI Eco47III Eco52I Eco81I EcoO65I EcoRI EcoRV EcoT22I FseI FspI HgaI Hin1I HincII HindII HindIII HpaI KasI KpnI KspI MamI MfeI MflI MluI MroI MseI MunI NaeI NarI NdeI NgoMIV NheI NotI NruI NsiI NspV PacI PaeR7I PflMI PinAI PmaCI PmeI PmlI PpuMI PshAI PshBI Psp1406I PvuI RsrII SacI SacII SalI SapI SbfI SexAI SfoI SfuI SmaI SnaBI SpeI SphI Sse8387I SspBI SspI StuI SwaI Tru9I Tsp509I Van91I XbaI XcmI XhoI XhoII XmaCI XmaI XmnI ################################### # ONE CUT ENZYMES ( 33 enzymes) # ################################### AfaI 831 GTAC GT^AC AflIII 453 ACATGT A^C[GA][CT]GT ApaI 534 GGGCCC GGGCC^C AspI 196 GACGGTGTC GAC[ACGT]^[ACGT][ACGT]GTC BalI 419 TGGCCA TGG^CCA BfaI 434 CTAG C^TAG BsaI 67 GAGACC GGTCTC(1/5) BseRI 519 CTCCTC GAGGAG(10/8) BsmBI 221 GAGACG CGTCTC(1/5) BsmFI 675 GTCCC GGGAC(10/14) BspLU11I 453 ACATGT A^CATGT DdeI 522 CTCAG C^T[ACGT]AG EarI 550 CTCTTC CTCTTC(1/4) EcoT14I 530 CCATGG C^C[AT][AT]GG HaeII 131 GGCGCT [GA]GCGC^[CT] Ksp632I 550 CTCTTC CTCTTC(1/4) MaeI 434 CTAG C^TAG MaeIII 461 GTGAC ^GT[ACGT]AC MluNI 419 TGGCCA TGG^CCA MscI 419 TGGCCA TGG^CCA MslI 454 CATGTGGGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG NcoI 530 CCATGG C^CATGG NspI 453 ACATGT [GA]CATG^[CT] PflFI 196 GACGGTGTC GAC[ACGT]^[ACGT][ACGT]GTC RsaI 831 GTAC GT^AC ScaI 830 AGTACT AGT^ACT SfiI 10 GGCCCCGACGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC SgrAI 127 CACCGGCG C[GA]^CCGG[CT]G SmlI 589 CTCAAG C^T[CT][GA]AG StyI 530 CCATGG C^C[AT][AT]GG TfiI 486 GAATC G^A[AT]TC Tsp45I 461 GTGAC ^GT[GC]AC Tth111I 196 GACGGTGTC GAC[ACGT]^[ACGT][ACGT]GTC ################################### # TWO CUT ENZYMES ( 21 enzymes) # ################################### Alw44I 210 GTGCAC G^TGCAC 847 GTGCAC ApaLI 210 GTGCAC G^TGCAC 847 GTGCAC AvaI 762 CCCGAG C^[CT]CG[GA]G 871 CCCGAG AvaII 360 GGTCC G^G[AT]CC 642 GGACC BanII 4 GAGCCC G[GA]GC[CT]^C 534 GGGCCC BbsI 579 GAAGAC GAAGAC(2/6) 157 GTCTTC BclI 293 TGATCA T^GATCA 506 TGATCA BcnI 7 CCCGG CC^[GC]GG 85 CCCGG BpuAI 579 GAAGAC GAAGAC(2/6) 157 GTCTTC BsoBI 762 CCCGAG C^[CT]CG[GA]G 871 CCCGAG BspHI 476 TCATGA T^CATGA 575 TCATGA BsrFI 128 ACCGGC [GA]^CCGG[CT] 783 GCCGGT Cac8I 134 GCTCGC GC[ACGT]^[ACGT]GC 880 GCCCGC Cfr10I 128 ACCGGC [GA]^CCGG[CT] 783 GCCGGT DrdI 148 GACATCCTCGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC 376 GACGAGGATGTC EcoNI 321 CCTGCGGGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG 645 CCTCATCAAGG FbaI 293 TGATCA T^GATCA 506 TGATCA HphI 333 GGTGA GGTGA(8/7) 460 GGTGA NciI 7 CCCGG CC^[GC]GG 85 CCCGG PvuII 115 CAGCTG CAG^CTG 823 CAGCTG RcaI 476 TCATGA T^CATGA 575 TCATGA ######################################### # 3 OR MORE CUT ENZYMES ( 62 enzymes) # ######################################### AccII AciI AluI AlwNI AspHI BbvI BglI BmyI BsaJI BsgI BsiHKAI BsiYI BslI BsmAI Bsp1286I BspMI BsrI BssKI BstF5I BstNI BstUI CfoI Cfr13I DpnII DraII DsaI EaeI EcoO109I EcoRII FauI Fnu4HI FokI HaeIII HapII HhaI HinP1I HinfI HpaII ItaI MaeII MboI MboII MnlI MspA1I MspI MvaI MvnI MwoI NdeII NlaIII NlaIV PleI PstI Sau3AI Sau96I ScrFI SfaNI SfcI TaqI TseI TspRI TthHB8I