(Circular) (Six-base) MAPSORT of: pFN21AE3540.seq Check: 2057 from: 1 to: 5611 pFN21AE3540 5611 bp With 182 enzymes: SgfI * July 6, 2012 17:34 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2101 2803 2101 Size: 702 4909 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3421 278 Size: 53 83 186 936 1885 2468 Fragments arranged by size: 2468 1885 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2954 1937 Size: 1017 4594 Acc65I G'GTAC_C Cuts at: 2932 2932 Size: 5611 AclI AA'CG_TT Cuts at: 1563 3738 4111 1563 Size: 2175 373 3063 Fragments arranged by size: 3063 2175 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2852 3674 5058 5326 1632 Size: 1220 822 1384 268 1917 Fragments arranged by size: 1917 1384 1220 822 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5414 AflIII A'CryG_T Cuts at: 1203 2458 4510 5487 1203 Size: 1255 2052 977 1327 Fragments arranged by size: 2052 1327 1255 977 AgeI A'CCGG_T Cuts at: 4442 4442 Size: 5611 AhdI GACnn_n'nnGTC Cuts at: 4340 4340 Size: 5611 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 5611 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 5611 AlwNI CAG_nnn'CTG Cuts at: 2101 2638 2683 2800 3354 4926 2101 Size: 537 45 117 554 1572 2786 Fragments arranged by size: 2786 1572 554 537 117 45 ApaI G_GGCC'C Cuts at: 2543 2543 Size: 5611 ApaLI G'TGCA_C Cuts at: 2215 2852 3668 4824 2215 Size: 637 816 1156 3002 Fragments arranged by size: 3002 1156 816 637 ApoI r'AATT_y Cuts at: 1493 2920 3146 3298 5407 1493 Size: 1427 226 152 2109 1697 Fragments arranged by size: 2109 1697 1427 226 152 AseI AT'TA_AT Cuts at: 160 1032 4165 160 Size: 872 3133 1606 Fragments arranged by size: 3133 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5611 AvaI C'yCGr_G Cuts at: 1870 1943 2767 2876 2936 1870 Size: 73 824 109 60 4545 Fragments arranged by size: 4545 824 109 73 60 BamHI G'GATC_C Cuts at: 2941 3308 2941 Size: 367 5244 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2932 4387 618 Size: 325 217 627 1145 1455 1842 Fragments arranged by size: 1842 1455 1145 627 325 217 BanII G_rGCy'C Cuts at: 1391 2013 2543 2930 1391 Size: 622 530 387 4072 Fragments arranged by size: 4072 622 530 387 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2155 2591 928 Size: 971 256 436 3948 Fragments arranged by size: 3948 971 436 256 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 3802 3836 1903 Size: 34 1865 34 3678 Fragments arranged by size: 3678 1865 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3503 4713 3503 Size: 1210 4401 BclI T'GATC_A Cuts at: 1548 2298 2511 1548 Size: 750 213 4648 Fragments arranged by size: 4648 750 213 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2022 2742 2891 Size: 107 122 71 631 605 350 720 149 Cuts at: 2891 4222 136 Size: 1331 1525 Fragments arranged by size: 1525 1331 720 631 605 350 149 122 107 71 BglII A'GATC_T Cuts at: 5606 5606 Size: 5611 BlpI GC'TnA_GC Cuts at: 3028 3028 Size: 5611 Bme1580I G_kGCm'C Cuts at: 1790 2219 2543 2856 3672 4828 1790 Size: 429 324 313 816 1156 2573 Fragments arranged by size: 2573 1156 816 429 324 313 BmgBI CAC'GTC Cuts at: 1318 2509 2851 1318 Size: 1191 342 4078 Fragments arranged by size: 4078 1191 342 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3342 4300 442 Size: 416 53 83 399 24 1925 958 1753 Fragments arranged by size: 1925 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5611 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 4271 1239 Size: 3032 2579 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2579 3736 4601 4899 2579 Size: 1157 865 298 3291 Fragments arranged by size: 3291 1157 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2066 4274 882 Size: 617 567 2208 2219 Fragments arranged by size: 2219 2208 617 567 BsaAI yAC'GTr Cuts at: 493 1204 3402 493 Size: 711 2198 2702 Fragments arranged by size: 2702 2198 711 BsaBI GATnn'nnATC Cuts at: 5605 5605 Size: 5611 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3418 3800 4435 275 Size: 53 83 186 936 1885 382 635 1451 Fragments arranged by size: 1885 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 4044 4442 4716 4863 5274 4044 Size: 398 274 147 411 4381 Fragments arranged by size: 4381 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2219 2249 2828 2858 1178 Size: 30 165 30 816 30 579 30 3931 Fragments arranged by size: 3931 816 579 165 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2515 1186 Size: 1329 4282 BseYI C'CCAG_C Cuts at: 2729 4814 5310 2729 Size: 2085 496 3030 Fragments arranged by size: 3030 2085 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2624 2695 2776 2813 1163 Size: 573 888 71 81 37 3961 Fragments arranged by size: 3961 888 573 81 71 37 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3061 3822 4850 1540 Size: 275 186 1060 761 1028 2301 Fragments arranged by size: 2301 1060 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2219 2774 2856 3672 3757 4828 2219 Size: 555 82 816 85 1071 3002 Fragments arranged by size: 3002 1071 816 555 85 82 BsmI GAATG_Cn' Cuts at: 3122 3215 3122 Size: 93 5518 BsmBI CGTCTCn'nnnn_ Cuts at: 2220 2220 Size: 5611 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2013 2219 2543 2774 2856 2930 3672 Size: 399 223 206 324 231 82 74 742 Cuts at: 3672 3757 4828 1391 Size: 85 1071 2174 Fragments arranged by size: 2174 1071 742 399 324 231 223 206 85 82 74 BspEI T'CCGG_A Cuts at: 5274 5274 Size: 5611 BspHI T'CATG_A Cuts at: 2481 2580 3500 5230 2481 Size: 99 920 1730 2862 Fragments arranged by size: 2862 1730 920 99 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2084 2101 2268 2803 2966 844 Size: 1240 17 167 535 163 3489 Fragments arranged by size: 3489 1240 535 167 163 17 BsrBI CCG'CTC Cuts at: 1394 1823 3498 1394 Size: 429 1675 3507 Fragments arranged by size: 3507 1675 429 BsrDI GCAATG_nn' Cuts at: 65 1248 4106 4280 65 Size: 1183 2858 174 1396 Fragments arranged by size: 2858 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2133 2788 4255 4442 1700 Size: 110 323 655 1467 187 2869 Fragments arranged by size: 2869 1467 655 323 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5611 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5611 BssSI C'ACGA_G Cuts at: 3671 4683 3671 Size: 1012 4599 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 5611 BstBI TT'CG_AA Cuts at: 4415 4415 Size: 5611 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5611 BstYI r'GATC_y Cuts at: 1972 2941 3308 3693 3710 5162 5248 5260 5606 Size: 969 367 385 17 1452 86 12 346 Cuts at: 5606 1972 Size: 1977 Fragments arranged by size: 1977 1452 969 385 367 346 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2004 2352 2361 2535 2691 513 Size: 552 939 348 9 174 156 3433 Fragments arranged by size: 3433 939 552 348 174 156 9 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 4872 Fragments arranged by size: 4872 683 56 BtsI GCAGTG_nn' Cuts at: 3127 3921 3941 3127 Size: 794 20 4797 Fragments arranged by size: 4797 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5611 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5576 DraI TTT'AAA Cuts at: 2915 2915 Size: 5611 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 2159 2387 4618 809 Size: 507 843 228 2231 1802 Fragments arranged by size: 2231 1802 843 507 228 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2092 2424 2734 Size: 54 1425 50 275 18 262 332 310 Cuts at: 2734 3058 3947 5576 8 Size: 324 889 1629 43 Fragments arranged by size: 1629 1425 889 332 324 310 275 262 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3058 1537 Size: 275 1246 4090 Fragments arranged by size: 4090 1246 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2561 3541 1353 Size: 1208 980 3423 Fragments arranged by size: 3423 1208 980 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2325 4198 4572 4718 387 Size: 1064 138 736 1873 374 146 1280 Fragments arranged by size: 1873 1280 1064 736 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2852 3674 4271 5058 5326 1239 Size: 393 1220 822 597 787 268 1524 Fragments arranged by size: 1524 1220 822 787 597 393 268 EcoNI CCTnn'n_nnAGG Cuts at: 2330 2654 2330 Size: 324 5287 EcoO109I rG'GnC_Cy Cuts at: 2098 2540 2893 2098 Size: 442 353 4816 Fragments arranged by size: 4816 442 353 EcoRI G'AATT_C Cuts at: 2920 3298 2920 Size: 378 5233 FspI TGC'GCA Cuts at: 4117 4117 Size: 5611 HaeII r_GCGC'y Cuts at: 1678 2140 4758 1678 Size: 462 2618 2531 Fragments arranged by size: 2618 2531 462 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2219 2251 4252 4284 4326 Size: 32 135 32 647 32 2001 32 42 Cuts at: 4326 4358 1373 Size: 32 2626 Fragments arranged by size: 2626 2001 647 135 42 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2955 3201 669 Size: 988 281 1017 246 3079 Fragments arranged by size: 3079 1017 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 5611 HpaI GTT'AAC Cuts at: 3201 3201 Size: 5611 KpnI G_GTAC'C Cuts at: 2936 2936 Size: 5611 MfeI C'AATT_G Cuts at: 3210 3210 Size: 5611 MluI A'CGCG_T Cuts at: 5487 5487 Size: 5611 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2178 2871 4725 4909 1305 Size: 873 693 1854 184 2007 Fragments arranged by size: 2007 1854 873 693 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2426 10 Size: 54 1425 343 594 3195 Fragments arranged by size: 3195 1425 594 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2463 3569 3928 4087 518 Size: 648 1297 1106 359 159 2042 Fragments arranged by size: 2042 1297 1106 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2122 2733 2800 2830 3027 3704 4852 5097 Size: 516 611 67 30 197 677 1148 245 Cuts at: 5097 1606 Size: 2120 Fragments arranged by size: 2120 1148 677 611 516 245 197 67 30 NcoI C'CATG_G Cuts at: 513 1065 2004 2535 513 Size: 552 939 531 3589 Fragments arranged by size: 3589 939 552 531 NdeI CA'TA_TG Cuts at: 387 387 Size: 5611 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5611 NotI GC'GGCC_GC Cuts at: 3058 3058 Size: 5611 NspI r_CATG'y Cuts at: 1132 1229 2462 2969 4514 1132 Size: 97 1233 507 1545 2229 Fragments arranged by size: 2229 1545 1233 507 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 5611 PciI A'CATG_T Cuts at: 2458 4510 2458 Size: 2052 3559 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 5611 PfoI T'CCnGG_A Cuts at: 2781 2781 Size: 5611 PmeI GTTT'AAAC Cuts at: 2915 2915 Size: 5611 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2048 2080 3680 3712 5193 5225 2048 Size: 32 1600 32 1481 32 2434 Fragments arranged by size: 2434 1600 1481 32 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5611 PsiI TTA'TAA Cuts at: 3181 3181 Size: 5611 PspOMI G'GGCC_C Cuts at: 2539 2539 Size: 5611 PstI C_TGCA'G Cuts at: 830 2081 2112 2273 2963 830 Size: 1251 31 161 690 3478 Fragments arranged by size: 3478 1251 690 161 31 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5611 PvuII CAG'CTG Cuts at: 2122 2830 2122 Size: 708 4903 SalI G'TCGA_C Cuts at: 1936 2953 1936 Size: 1017 4594 SbfI CC_TGCA'GG Cuts at: 2963 2963 Size: 5611 ScaI AGT'ACT Cuts at: 2837 3859 5571 2837 Size: 1022 1712 2877 Fragments arranged by size: 2877 1712 1022 SfcI C'TryA_G Cuts at: 826 1045 2077 2108 2269 2959 4094 4775 4966 Size: 219 1032 31 161 690 1135 681 191 Cuts at: 4966 826 Size: 1471 Fragments arranged by size: 1471 1135 1032 690 681 219 191 161 31 SfiI GGCCn_nnn'nGGCC Cuts at: 2022 2022 Size: 5611 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5611 SgrAI Cr'CCGG_yG Cuts at: 1700 2133 1700 Size: 433 5178 SmaI CCC'GGG Cuts at: 1872 2938 1872 Size: 1066 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2594 3715 4616 4878 820 Size: 197 926 651 1121 901 262 1553 Fragments arranged by size: 1553 1121 926 901 651 262 197 SnaBI TAC'GTA Cuts at: 493 3402 493 Size: 2909 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5611 SphI G_CATG'C Cuts at: 1132 2969 1132 Size: 1837 3774 SspI AAT'ATT Cuts at: 5 52 3535 5 Size: 47 3483 2081 Fragments arranged by size: 3483 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 5611 StyI C'CwwG_G Cuts at: 513 1065 2004 2535 3050 513 Size: 552 939 531 515 3074 Fragments arranged by size: 3074 939 552 531 515 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2453 3638 3655 3808 3993 4438 325 Size: 1255 873 1185 17 153 185 445 1498 Fragments arranged by size: 1498 1255 1185 873 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2835 3857 5569 Size: 274 80 33 51 1443 858 1022 1712 Cuts at: 5569 96 Size: 138 Fragments arranged by size: 1712 1443 1022 858 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 5571 5603 5571 Size: 32 5579 Tth111I GACn'n_nGTC Cuts at: 1316 2204 1316 Size: 888 4723 XbaI T'CTAG_A Cuts at: 2947 2947 Size: 5611 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5611 XmaI C'CCGG_G Cuts at: 1870 2936 1870 Size: 1066 4545 XmnI GAAnn'nnTTC Cuts at: 3740 3740 Size: 5611 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3419 276 Size: 53 83 186 936 1885 2468 Fragments arranged by size: 2468 1885 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoNI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PshAI PsiI PspOMI PstI PvuI PvuII SalI SbfI ScaI SfcI SfiI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI AloI AscI AvrII BaeI BbeI BbvCI BfrBI BplI Bpu10I BsiWI BstXI BstZ17I Bsu36I DraIII EcoICRI EcoRV FalI FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PmlI PpuMI PspXI PsrI RsrII SacI SacII SanDI SapI SexAI SfoI SrfI SwaI XcmI