Restriction map for
pFN21AE9519
(Circular) (Six-base) MAPSORT of: pFN21AE9519.seq Check: 4423 from: 1 to: 5779
pFN21AE9519 5779 bp
With 182 enzymes: SgfI *
July 6, 2012 17:34 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3589 278
Size: 53 83 186 936 2053 2468
Fragments arranged by size:
2468 2053 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2478 3122 1937
Size: 541 644 4594
Fragments arranged by size:
4594 644 541
Acc65I G'GTAC_C
Cuts at: 3100 3100
Size: 5779
AclI AA'CG_TT
Cuts at: 1563 3906 4279 1563
Size: 2343 373 3063
Fragments arranged by size:
3063 2343 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2824 3842 5226 5494 1632
Size: 1192 1018 1384 268 1917
Fragments arranged by size:
1917 1384 1192 1018 268
AfeI AGC'GCT
Cuts at: 2360 2757 2360
Size: 397 5382
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5582
AflIII A'CryG_T
Cuts at: 1203 2301 4678 5655 1203
Size: 1098 2377 977 1327
Fragments arranged by size:
2377 1327 1098 977
AgeI A'CCGG_T
Cuts at: 4610 4610
Size: 5779
AhdI GACnn_n'nnGTC
Cuts at: 4508 4508
Size: 5779
AleI CACnn'nnGTG
Cuts at: 1166 2183 1166
Size: 1017 4762
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5779
AlwNI CAG_nnn'CTG
Cuts at: 3522 5094 3522
Size: 1572 4207
ApaI G_GGCC'C
Cuts at: 2719 2719
Size: 5779
ApaLI G'TGCA_C
Cuts at: 3836 4992 3836
Size: 1156 4623
ApoI r'AATT_y
Cuts at: 1493 3088 3314 3466 5575 1493
Size: 1595 226 152 2109 1697
Fragments arranged by size:
2109 1697 1595 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4333 160
Size: 872 3301 1606
Fragments arranged by size:
3301 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5779
AvaI C'yCGr_G
Cuts at: 1870 1943 2895 2946 3037 3104 1870
Size: 73 952 51 91 67 4545
Fragments arranged by size:
4545 952 91 73 67 51
BamHI G'GATC_C
Cuts at: 3109 3476 3109
Size: 367 5412
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2389 2633 3100 4555 618
Size: 325 217 627 602 244 467 1455 1842
Fragments arranged by size:
1842 1455 627 602 467 325 244 217
BanII G_rGCy'C
Cuts at: 1391 2648 2719 2985 3098 1391
Size: 1257 71 266 113 4072
Fragments arranged by size:
4072 1257 266 113 71
BbeI G_GCGC'C
Cuts at: 2393 2637 2393
Size: 244 5535
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2060 2863 928
Size: 971 161 803 3844
Fragments arranged by size:
3844 971 803 161
BbvCI CC'TCA_GC
Cuts at: 2997 2997
Size: 5779
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 2104 2138 3970 4004 1903
Size: 34 167 34 1832 34 3678
Fragments arranged by size:
3678 1832 167 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3671 4881 3671
Size: 1210 4569
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5779
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2280 2468 2804
Size: 107 122 71 631 605 608 188 336
Cuts at: 2804 4390 136
Size: 1586 1525
Fragments arranged by size:
1586 1525 631 608 605 336 188 122
107 71
BglII A'GATC_T
Cuts at: 2884 5774 2884
Size: 2890 2889
BlpI GC'TnA_GC
Cuts at: 2045 2312 2348 3196 2045
Size: 267 36 848 4628
Fragments arranged by size:
4628 848 267 36
Bme1580I G_kGCm'C
Cuts at: 1790 2719 3840 4996 1790
Size: 929 1121 1156 2573
Fragments arranged by size:
2573 1156 1121 929
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5779
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3510 4468 442
Size: 416 53 83 399 24 2093 958 1753
Fragments arranged by size:
2093 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5779
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2576 2901 4439 1239
Size: 1337 325 1538 2579
Fragments arranged by size:
2579 1538 1337 325
Bpu10I CC'TnA_GC
Cuts at: 2549 2997 2549
Size: 448 5331
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3904 4769 5067 3904
Size: 865 298 4616
Fragments arranged by size:
4616 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 3027 4442 882
Size: 617 1528 1415 2219
Fragments arranged by size:
2219 1528 1415 617
BsaAI yAC'GTr
Cuts at: 493 1204 2302 3570 493
Size: 711 1098 1268 2702
Fragments arranged by size:
2702 1268 1098 711
BsaBI GATnn'nnATC
Cuts at: 5773 5773
Size: 5779
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2390 2634 3586 3968
Size: 53 83 186 936 857 244 952 382
Cuts at: 3968 4603 275
Size: 635 1451
Fragments arranged by size:
1451 952 936 857 635 382 244 186
83 53
BsaWI w'CCGG_w
Cuts at: 2767 3064 4212 4610 4884 5031 5442 2767
Size: 297 1148 398 274 147 411 3104
Fragments arranged by size:
3104 1148 411 398 297 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 5554
Fragments arranged by size:
5554 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 1186
Size: 5779
BseYI C'CCAG_C
Cuts at: 2101 2269 2640 2788 4982 5478 2101
Size: 168 371 148 2194 496 2402
Fragments arranged by size:
2402 2194 496 371 168 148
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2139 2174 1163
Size: 573 403 35 4768
Fragments arranged by size:
4768 573 403 35
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2168 2472 2751 2959 3229 3990
Size: 275 186 167 304 279 208 270 761
Cuts at: 3990 5018 1540
Size: 1028 2301
Fragments arranged by size:
2301 1028 761 304 279 275 270 208
186 167
BsiHKAI G_wGCw'C
Cuts at: 2865 2985 3840 3925 4996 2865
Size: 120 855 85 1071 3648
Fragments arranged by size:
3648 1071 855 120 85
BsmI GAATG_Cn'
Cuts at: 3290 3383 3290
Size: 93 5686
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2648 2719 2865 2985 3098 3840 3925
Size: 399 858 71 146 120 113 742 85
Cuts at: 3925 4996 1391
Size: 1071 2174
Fragments arranged by size:
2174 1071 858 742 399 146 120 113
85 71
BspEI T'CCGG_A
Cuts at: 3064 5442 3064
Size: 2378 3401
BspHI T'CATG_A
Cuts at: 3668 5398 3668
Size: 1730 4049
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2891 3134 844
Size: 2047 243 3489
Fragments arranged by size:
3489 2047 243
BsrBI CCG'CTC
Cuts at: 1394 1823 2233 2816 2961 3666 1394
Size: 429 410 583 145 705 3507
Fragments arranged by size:
3507 705 583 429 410 145
BsrDI GCAATG_nn'
Cuts at: 65 1248 3034 4274 4448 65
Size: 1183 1786 1240 174 1396
Fragments arranged by size:
1786 1396 1240 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2339 2527 2673 2798 4423 4610 1700
Size: 110 529 188 146 125 1625 187 2869
Fragments arranged by size:
2869 1625 529 188 187 146 125 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5779
BssHII G'CGCG_C
Cuts at: 1925 2307 1925
Size: 382 5397
BssSI C'ACGA_G
Cuts at: 3839 4851 3839
Size: 1012 4767
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5779
BstBI TT'CG_AA
Cuts at: 2928 4583 2928
Size: 1655 4124
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5779
BstYI r'GATC_y
Cuts at: 1972 2884 3109 3476 3861 3878 5330 5416 5428
Size: 912 225 367 385 17 1452 86 12
Cuts at: 5428 5774 1972
Size: 346 1977
Fragments arranged by size:
1977 1452 912 385 367 346 225 86
17 12
BtgI C'CryG_G
Cuts at: 513 1065 2976 3022 513
Size: 552 1911 46 3270
Fragments arranged by size:
3270 1911 552 46
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5040
Fragments arranged by size:
5040 683 56
BtsI GCAGTG_nn'
Cuts at: 2039 3295 4089 4109 2039
Size: 1256 794 20 3709
Fragments arranged by size:
3709 1256 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5779
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5744
DraI TTT'AAA
Cuts at: 3083 3083
Size: 5779
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4786 809
Size: 507 3470 1802
Fragments arranged by size:
3470 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2175 2202 2264
Size: 54 1425 50 275 18 345 27 62
Cuts at: 2264 2445 2469 2800 2956 3226 4115 5744 8
Size: 181 24 331 156 270 889 1629 43
Fragments arranged by size:
1629 1425 889 345 331 275 270 181
156 62 54 50 43 27 24 18
EagI C'GGCC_G
Cuts at: 1537 1812 2469 2956 3226 1537
Size: 275 657 487 270 4090
Fragments arranged by size:
4090 657 487 275 270
EarI CTCTTCn'nnn_
Cuts at: 1353 2930 3709 1353
Size: 1577 779 3423
Fragments arranged by size:
3423 1577 779
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2808 4366 4740 4886 387
Size: 1064 138 1219 1558 374 146 1280
Fragments arranged by size:
1558 1280 1219 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 2983 2983
Size: 5779
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2576 2824 2901 3842 4439 5226 5494
Size: 393 944 248 77 941 597 787 268
Cuts at: 5494 1239
Size: 1524
Fragments arranged by size:
1524 944 941 787 597 393 268 248
77
EcoNI CCTnn'n_nnAGG
Cuts at: 2741 2741
Size: 5779
EcoO109I rG'GnC_Cy
Cuts at: 2716 2775 2903 3073 2716
Size: 59 128 170 5422
Fragments arranged by size:
5422 170 128 59
EcoRI G'AATT_C
Cuts at: 3088 3466 3088
Size: 378 5401
FspI TGC'GCA
Cuts at: 2402 4285 2402
Size: 1883 3896
HaeII r_GCGC'y
Cuts at: 1678 2099 2201 2362 2393 2637 2688 2759 4926
Size: 421 102 161 31 244 51 71 2167
Cuts at: 4926 1678
Size: 2531
Fragments arranged by size:
2531 2167 421 244 161 102 71 51
31
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 4420 4452 4494 4526 1373
Size: 32 135 32 2848 32 42 32 2626
Fragments arranged by size:
2848 2626 135 42 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2734 2854 3123 3369 669
Size: 988 281 796 120 269 246 3079
Fragments arranged by size:
3079 988 796 281 269 246 120
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5779
HpaI GTT'AAC
Cuts at: 3369 3369
Size: 5779
KasI G'GCGC_C
Cuts at: 2389 2633 2389
Size: 244 5535
KpnI G_GTAC'C
Cuts at: 3104 3104
Size: 5779
MfeI C'AATT_G
Cuts at: 3378 3378
Size: 5779
MluI A'CGCG_T
Cuts at: 5655 5655
Size: 5779
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 4893 5077 1305
Size: 3588 184 2007
Fragments arranged by size:
3588 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 3957
Fragments arranged by size:
3957 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2183 3737 4096 4255 518
Size: 648 1017 1554 359 159 2042
Fragments arranged by size:
2042 1554 1017 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2170 2269 2512 2640 2879 3001 3024 3195
Size: 564 99 243 128 239 122 23 171
Cuts at: 3195 3872 5020 5265 1606
Size: 677 1148 245 2120
Fragments arranged by size:
2120 1148 677 564 245 243 239 171
128 122 99 23
NaeI GCC'GGC
Cuts at: 2675 2800 2675
Size: 125 5654
NarI GG'CG_CC
Cuts at: 2390 2634 2390
Size: 244 5535
NcoI C'CATG_G
Cuts at: 513 1065 2976 513
Size: 552 1911 3316
Fragments arranged by size:
3316 1911 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5779
NgoMIV G'CCGG_C
Cuts at: 2673 2798 2673
Size: 125 5654
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5779
NotI GC'GGCC_GC
Cuts at: 2956 3226 2956
Size: 270 5509
NspI r_CATG'y
Cuts at: 1132 1229 2320 3137 4682 1132
Size: 97 1091 817 1545 2229
Fragments arranged by size:
2229 1545 1091 817 97
PasI CC'CwG_GG
Cuts at: 1348 2027 1348
Size: 679 5100
PciI A'CATG_T
Cuts at: 4678 4678
Size: 5779
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 5779
PmeI GTTT'AAAC
Cuts at: 3083 3083
Size: 5779
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2980 3012 3848 3880 5361 5393 2980
Size: 32 836 32 1481 32 3366
Fragments arranged by size:
3366 1481 836 32 32 32
PpuMI rG'GwC_Cy
Cuts at: 2903 3073 2903
Size: 170 5609
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5779
PsiI TTA'TAA
Cuts at: 3349 3349
Size: 5779
PspOMI G'GGCC_C
Cuts at: 2715 2715
Size: 5779
PstI C_TGCA'G
Cuts at: 830 2358 2745 3131 830
Size: 1528 387 386 3478
Fragments arranged by size:
3478 1528 387 386
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5779
PvuII CAG'CTG
Cuts at: 2512 3001 2512
Size: 489 5290
RsrII CG'GwC_CG
Cuts at: 3062 3062
Size: 5779
SacI G_AGCT'C
Cuts at: 2985 2985
Size: 5779
SacII CC_GC'GG
Cuts at: 3025 3025
Size: 5779
SalI G'TCGA_C
Cuts at: 1936 3121 1936
Size: 1185 4594
SapI GCTCTTCn'nnn_
Cuts at: 2930 2930
Size: 5779
SbfI CC_TGCA'GG
Cuts at: 3131 3131
Size: 5779
ScaI AGT'ACT
Cuts at: 4027 5739 4027
Size: 1712 4067
SfcI C'TryA_G
Cuts at: 826 1045 2354 2605 2741 3127 4262 4943 5134
Size: 219 1309 251 136 386 1135 681 191
Cuts at: 5134 826
Size: 1471
Fragments arranged by size:
1471 1309 1135 681 386 251 219 191
136
SfoI GGC'GCC
Cuts at: 2391 2635 2391
Size: 244 5535
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5779
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5779
SmaI CCC'GGG
Cuts at: 1872 2897 3039 3106 1872
Size: 1025 142 67 4545
Fragments arranged by size:
4545 1025 142 67
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3883 4784 5046 820
Size: 197 926 1940 901 262 1553
Fragments arranged by size:
1940 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3570 493
Size: 3077 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5779
SphI G_CATG'C
Cuts at: 1132 3137 1132
Size: 2005 3774
SspI AAT'ATT
Cuts at: 5 52 3703 5
Size: 47 3651 2081
Fragments arranged by size:
3651 2081 47
StuI AGG'CCT
Cuts at: 1517 2261 1517
Size: 744 5035
StyI C'CwwG_G
Cuts at: 513 1065 2778 2829 2976 3218 513
Size: 552 1713 51 147 242 3074
Fragments arranged by size:
3074 1713 552 242 147 51
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 1999 3806 3823 3976 4161 4606 325
Size: 1255 419 1807 17 153 185 445 1498
Fragments arranged by size:
1807 1498 1255 445 419 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2295 4025 5737
Size: 274 80 33 51 1443 318 1730 1712
Cuts at: 5737 96
Size: 138
Fragments arranged by size:
1730 1712 1443 318 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5739 5771 5739
Size: 32 5747
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5779
XbaI T'CTAG_A
Cuts at: 3115 3115
Size: 5779
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5779
XmaI C'CCGG_G
Cuts at: 1870 2895 3037 3104 1870
Size: 1025 142 67 4545
Fragments arranged by size:
4545 1025 142 67
XmnI GAAnn'nnTTC
Cuts at: 3908 3908
Size: 5779
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3587 276
Size: 53 83 186 936 2053 2468
Fragments arranged by size:
2468 2053 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AfeI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI
AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI
BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I
BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI
BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI
BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI
EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI
FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI
MfeI MluI MmeI MscI MslI MspA1I NaeI NarI
NcoI NdeI NgoMIV NheI NotI NspI PasI PciI
PflMI PmeI PpiI PpuMI PshAI PsiI PspOMI PstI
PvuI PvuII RsrII SacI SacII SalI SapI SbfI
ScaI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI
SpeI SphI SspI StuI StyI TaqII TatI TstI
Tth111I XbaI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AloI AscI AvrII BaeI BfrBI BplI BsiWI
BsmBI BstXI BstZ17I Bsu36I DraIII EcoRV FalI FseI
FspAI NruI NsiI PacI PfoI PmlI PspXI PsrI
SanDI SexAI SfiI SrfI SwaI XcmI