(Linear) (Six-base) MAPSORT of: KIEE9519.seq Check: 819 from: 1 to: 1074 KIEE9519.orf With 182 enzymes: SgfI * July 3, 2012 19:02 .. AccI GT'mk_AC Cuts at: 0 473 1074 Size: 473 601 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 0 819 1074 Size: 819 255 AfeI AGC'GCT Cuts at: 0 355 752 1074 Size: 355 397 322 Fragments arranged by size: 397 355 322 AflIII A'CryG_T Cuts at: 0 296 1074 Size: 296 778 AleI CACnn'nnGTG Cuts at: 0 178 1074 Size: 178 896 ApaI G_GGCC'C Cuts at: 0 714 1074 Size: 714 360 AvaI C'yCGr_G Cuts at: 0 890 941 1032 1074 Size: 890 51 91 42 Fragments arranged by size: 890 91 51 42 BanI G'GyrC_C Cuts at: 0 384 628 1074 Size: 384 244 446 Fragments arranged by size: 446 384 244 BanII G_rGCy'C Cuts at: 0 643 714 980 1074 Size: 643 71 266 94 Fragments arranged by size: 643 266 94 71 BbeI G_GCGC'C Cuts at: 0 388 632 1074 Size: 388 244 442 Fragments arranged by size: 442 388 244 BbsI GAAGACnn'nnnn_ Cuts at: 0 55 858 1074 Size: 55 803 216 Fragments arranged by size: 803 216 55 BbvCI CC'TCA_GC Cuts at: 0 992 1074 Size: 992 82 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 0 99 133 1074 Size: 99 34 941 Fragments arranged by size: 941 99 34 BglI GCCn_nnn'nGGC Cuts at: 0 275 463 799 1074 Size: 275 188 336 275 Fragments arranged by size: 336 275 275 188 BglII A'GATC_T Cuts at: 0 879 1074 Size: 879 195 BlpI GC'TnA_GC Cuts at: 0 40 307 343 1074 Size: 40 267 36 731 Fragments arranged by size: 731 267 40 36 Bme1580I G_kGCm'C Cuts at: 0 714 1074 Size: 714 360 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 571 896 1074 Size: 571 325 178 Fragments arranged by size: 571 325 178 Bpu10I CC'TnA_GC Cuts at: 0 544 992 1074 Size: 544 448 82 Fragments arranged by size: 544 448 82 BsaI GGTCTCn'nnnn_ Cuts at: 0 1022 1074 Size: 1022 52 BsaAI yAC'GTr Cuts at: 0 297 1074 Size: 297 777 BsaHI Gr'CG_yC Cuts at: 0 385 629 1074 Size: 385 244 445 Fragments arranged by size: 445 385 244 BsaWI w'CCGG_w Cuts at: 0 762 1059 1074 Size: 762 297 15 Fragments arranged by size: 762 297 15 BseYI C'CCAG_C Cuts at: 0 96 264 635 783 1074 Size: 96 168 371 148 291 Fragments arranged by size: 371 291 168 148 96 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 0 134 169 1074 Size: 134 35 905 Fragments arranged by size: 905 134 35 BsiEI CG_ry'CG Cuts at: 0 163 467 746 954 1074 Size: 163 304 279 208 120 Fragments arranged by size: 304 279 208 163 120 BsiHKAI G_wGCw'C Cuts at: 0 860 980 1074 Size: 860 120 94 Fragments arranged by size: 860 120 94 Bsp1286I G_dGCh'C Cuts at: 0 643 714 860 980 1074 Size: 643 71 146 120 94 Fragments arranged by size: 643 146 120 94 71 BspEI T'CCGG_A Cuts at: 0 1059 1074 Size: 1059 15 BspMI ACCTGCnnnn'nnnn_ Cuts at: 0 886 1074 Size: 886 188 BsrBI CCG'CTC Cuts at: 0 228 811 956 1074 Size: 228 583 145 118 Fragments arranged by size: 583 228 145 118 BsrDI GCAATG_nn' Cuts at: 0 1029 1074 Size: 1029 45 BsrFI r'CCGG_y Cuts at: 0 334 522 668 793 1074 Size: 334 188 146 125 281 Fragments arranged by size: 334 281 188 146 125 BssHII G'CGCG_C Cuts at: 0 302 1074 Size: 302 772 BstBI TT'CG_AA Cuts at: 0 923 1074 Size: 923 151 BstYI r'GATC_y Cuts at: 0 879 1074 Size: 879 195 BtgI C'CryG_G Cuts at: 0 971 1017 1074 Size: 971 46 57 Fragments arranged by size: 971 57 46 BtsI GCAGTG_nn' Cuts at: 0 34 1074 Size: 34 1040 EaeI y'GGCC_r Cuts at: 0 170 197 259 440 464 795 951 1074 Size: 170 27 62 181 24 331 156 123 Fragments arranged by size: 331 181 170 156 123 62 27 24 EagI C'GGCC_G Cuts at: 0 464 951 1074 Size: 464 487 123 Fragments arranged by size: 487 464 123 EarI CTCTTCn'nnn_ Cuts at: 0 925 1074 Size: 925 149 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 0 803 1074 Size: 803 271 EcoICRI GAG'CTC Cuts at: 0 978 1074 Size: 978 96 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 0 571 819 896 1074 Size: 571 248 77 178 Fragments arranged by size: 571 248 178 77 EcoNI CCTnn'n_nnAGG Cuts at: 0 736 1074 Size: 736 338 EcoO109I rG'GnC_Cy Cuts at: 0 711 770 898 1068 1074 Size: 711 59 128 170 6 Fragments arranged by size: 711 170 128 59 6 FspI TGC'GCA Cuts at: 0 397 1074 Size: 397 677 HaeII r_GCGC'y Cuts at: 0 94 196 357 388 632 683 754 1074 Size: 94 102 161 31 244 51 71 320 Fragments arranged by size: 320 244 161 102 94 71 51 31 HincII GTy'rAC Cuts at: 0 729 849 1074 Size: 729 120 225 Fragments arranged by size: 729 225 120 KasI G'GCGC_C Cuts at: 0 384 628 1074 Size: 384 244 446 Fragments arranged by size: 446 384 244 MslI CAynn'nnrTG Cuts at: 0 178 1074 Size: 178 896 MspA1I CmG'CkG Cuts at: 0 165 264 507 635 874 996 1019 1074 Size: 165 99 243 128 239 122 23 55 Fragments arranged by size: 243 239 165 128 122 99 55 23 NaeI GCC'GGC Cuts at: 0 670 795 1074 Size: 670 125 279 Fragments arranged by size: 670 279 125 NarI GG'CG_CC Cuts at: 0 385 629 1074 Size: 385 244 445 Fragments arranged by size: 445 385 244 NcoI C'CATG_G Cuts at: 0 971 1074 Size: 971 103 NgoMIV G'CCGG_C Cuts at: 0 668 793 1074 Size: 668 125 281 Fragments arranged by size: 668 281 125 NotI GC'GGCC_GC Cuts at: 0 951 1074 Size: 951 123 NspI r_CATG'y Cuts at: 0 315 1074 Size: 315 759 PasI CC'CwG_GG Cuts at: 0 22 1074 Size: 22 1052 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 0 975 1007 1074 Size: 975 32 67 Fragments arranged by size: 975 67 32 PpuMI rG'GwC_Cy Cuts at: 0 898 1068 1074 Size: 898 170 6 Fragments arranged by size: 898 170 6 PspOMI G'GGCC_C Cuts at: 0 710 1074 Size: 710 364 PstI C_TGCA'G Cuts at: 0 353 740 1074 Size: 353 387 334 Fragments arranged by size: 387 353 334 PvuII CAG'CTG Cuts at: 0 507 996 1074 Size: 507 489 78 Fragments arranged by size: 507 489 78 RsrII CG'GwC_CG Cuts at: 0 1057 1074 Size: 1057 17 SacI G_AGCT'C Cuts at: 0 980 1074 Size: 980 94 SacII CC_GC'GG Cuts at: 0 1020 1074 Size: 1020 54 SapI GCTCTTCn'nnn_ Cuts at: 0 925 1074 Size: 925 149 SfcI C'TryA_G Cuts at: 0 349 600 736 1074 Size: 349 251 136 338 Fragments arranged by size: 349 338 251 136 SfoI GGC'GCC Cuts at: 0 386 630 1074 Size: 386 244 444 Fragments arranged by size: 444 386 244 SmaI CCC'GGG Cuts at: 0 892 1034 1074 Size: 892 142 40 Fragments arranged by size: 892 142 40 StuI AGG'CCT Cuts at: 0 256 1074 Size: 256 818 StyI C'CwwG_G Cuts at: 0 773 824 971 1074 Size: 773 51 147 103 Fragments arranged by size: 773 147 103 51 TatI w'GTAC_w Cuts at: 0 290 1074 Size: 290 784 XmaI C'CCGG_G Cuts at: 0 890 1032 1074 Size: 890 142 42 Fragments arranged by size: 890 142 42 Enzymes that do cut: AccI AcuI AfeI AflIII AleI ApaI AvaI BanI BanII BbeI BbsI BbvCI BcgI BglI BglII BlpI Bme1580I BpmI Bpu10I BsaI BsaAI BsaHI BsaWI BseYI BsgI BsiEI BsiHKAI Bsp1286I BspEI BspMI BsrBI BsrDI BsrFI BssHII BstBI BstYI BtgI BtsI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I FspI HaeII HincII KasI MslI MspA1I NaeI NarI NcoI NgoMIV NotI NspI PasI PpiI PpuMI PspOMI PstI PvuII RsrII SacI SacII SapI SfcI SfoI SmaI StuI StyI TatI XmaI Enzymes that do not cut: AarI AatII Acc65I AclI AflII AgeI AhdI AlfI AloI AlwNI ApaLI ApoI AscI AseI AsiSI AvrII BaeI BamHI BciVI BclI BfrBI BmgBI BmrI BmtI BplI BpuEI BsaBI BsaXI BseRI BsiWI BsmI BsmBI BspHI BsrGI BssSI BstAPI BstEII BstXI BstZ17I Bsu36I BtgZI ClaI CspCI DraI DraIII DrdI EcoRI EcoRV FalI FseI FspAI Hin4I HindIII HpaI KpnI MfeI MluI MmeI MscI NdeI NheI NruI NsiI PacI PciI PflMI PfoI PmeI PmlI PshAI PsiI PspXI PsrI PvuI SalI SanDI SbfI ScaI SexAI SfiI SgfI SgrAI SmlI SnaBI SpeI SphI SrfI SspI SwaI TaqII TstI Tth111I XbaI XcmI XhoI XmnI ZraI