################################## # NO CUT ENZYMES ( 153 enzymes) # ################################## AatII AccII AccIII AclI AcyI AflII AgeI AhdI AlwI AlwNI AocI Aor51HI ApaI AscI AseI AsnI AspEI AspI AvaI AvaII AviII AvrII BalI BamHI BbeI BbrPI BbvCI BciVI BcnI BfaI BfrI BglII BlnI BlpI Bpu1102I BsaBI BsaHI BsaI BsaWI BseAI BsgI BsiWI BsoBI Bsp1407I BspDI BspEI BspHI BsrBI BsrDI BsrFI BsrGI BssHII Bst1107I BstAPI BstBI BstEII BstPI BstUI BstYI BstZ17I Bsu36I CelII Cfr10I ClaI CpoI DraI DraII DraIII DrdI EaeI EagI Eam1105I EclXI Eco47III Eco52I Eco81I EcoNI EcoO109I EcoO65I EcoRV EcoT22I FauI FseI FspI HaeII HapII HgaI Hin1I HpaI HpaII KasI KspI MaeI MamI MfeI MflI MluI MluNI MroI MscI MseI MspI MunI MvnI NaeI NarI NciI NdeI NgoMIV NheI NotI NruI NsiI NspV PacI PaeR7I PflFI PinAI PmaCI PmeI PmlI PpuMI PshBI Psp1406I RcaI RsrII SacII SalI SapI SbfI ScaI SexAI SfiI SfoI SfuI SgrAI SmaI SmlI SnaBI SpeI SphI Sse8387I SspBI SspI SwaI Tru9I Tth111I XbaI XcmI XhoI XhoII XmaCI XmaI ################################### # ONE CUT ENZYMES ( 35 enzymes) # ################################### Acc65I 199 GGTACC G^GTACC AccI 242 GTAGAC GT^[AC][GT]AC AcsI 794 GAATTC [GA]^AATT[CT] AflIII 726 ACATGT A^C[GA][CT]GT Alw44I 452 GTGCAC G^TGCAC ApaLI 452 GTGCAC G^TGCAC ApoI 794 GAATTC [GA]^AATT[CT] Asp718I 199 GGTACC G^GTACC BanI 199 GGTACC G^G[CT][GA]CC BbsI 580 GAAGAC GAAGAC(2/6) BglI 1159 GCCCCTGGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC BpuAI 580 GAAGAC GAAGAC(2/6) BsaAI 962 TACGTG [CT]AC^GT[GA] BsiEI 549 CGATCG CG[GA][CT]^CG BsmBI 771 CGTCTC CGTCTC(1/5) BsmI 837 GAATGC GAATGC(1/-1) BspLU11I 726 ACATGT A^CATGT BssSI 535 CTCGTG CACGAG(-5/-1) BstXI 530 CCATCCTCGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG Cfr13I 219 GGGCC G^G[ACGT]CC EcoRI 794 GAATTC G^AATTC HincII 934 GTCAAC GT[CT]^[GA]AC HindII 934 GTCAAC GT[CT]^[GA]AC HindIII 1124 AAGCTT A^AGCTT HphI 111 GGTGA GGTGA(8/7) KpnI 199 GGTACC GGTAC^C MaeIII 1228 GTGAC ^GT[ACGT]AC NspI 726 ACATGT [GA]CATG^[CT] PflMI 1279 CCAGCGAGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG PvuI 549 CGATCG CGAT^CG PvuII 574 CAGCTG CAG^CTG Sau96I 219 GGGCC G^G[ACGT]CC StuI 167 AGGCCT AGG^CCT Tsp45I 1228 GTGAC ^GT[GC]AC Van91I 1279 CCAGCGAGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG ################################### # TWO CUT ENZYMES ( 23 enzymes) # ################################### AfaI 200 GTAC GT^AC 421 GTAC Asp700I 921 GAACCACTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC 1078 GAAAATGTTC BanII 511 GAGCTC G[GA]GC[CT]^C 1132 GAGCTC BcgI 223 CGATTCCGCTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10) 590 GCAAAACTATCG BsmAI 498 GAGAC GTCTC(1/5) 772 GTCTC BsmFI 1263 GGGAC GGGAC(10/14) 77 GTCCC CfoI 5 GCGC GCG^C 390 GCGC DsaI 47 CCATGG C^C[GA][CT]GG 206 CCATGG EarI 382 GAAGAG CTCTTC(1/4) 1069 GAAGAG EcoT14I 47 CCATGG C^C[AT][AT]GG 206 CCATGG HhaI 5 GCGC GCG^C 390 GCGC HinP1I 5 GCGC G^CGC 390 GCGC Ksp632I 382 GAAGAG CTCTTC(1/4) 1069 GAAGAG MaeII 770 ACGT A^CGT 963 ACGT MspA1I 228 CCGCTG C[AC]G^C[GT]G 574 CAGCTG NcoI 47 CCATGG C^CATGG 206 CCATGG PshAI 500 GACAGAAGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC 687 GACCCAAGTC RsaI 200 GTAC GT^AC 421 GTAC SacI 511 GAGCTC GAGCT^C 1132 GAGCTC StyI 47 CCATGG C^C[AT][AT]GG 206 CCATGG TaqI 552 TCGA T^CGA 599 TCGA TthHB8I 552 TCGA T^CGA 599 TCGA XmnI 921 GAACCACTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC 1078 GAAAATGTTC ######################################### # 3 OR MORE CUT ENZYMES ( 47 enzymes) # ######################################### AciI AluI AspHI BbvI BclI BmyI BpmI BsaJI BseRI BsiHKAI BsiYI BslI Bsp1286I BspMI BsrI BssKI BstF5I BstNI Cac8I DdeI DpnII EcoRII FbaI Fnu4HI FokI HaeIII HinfI ItaI MboI MboII MnlI MslI MvaI MwoI NdeII NlaIII NlaIV PleI PstI Sau3AI ScrFI SfaNI SfcI TfiI TseI Tsp509I TspRI