################################## # NO CUT ENZYMES ( 167 enzymes) # ################################## AatII Acc65I AccI AccIII AclI AcsI AfaI AflII AflIII AgeI AhdI Alw44I AlwNI AocI Aor51HI ApaI ApaLI ApoI AscI AseI AsnI Asp700I Asp718I AspEI AspI AvaI AviII AvrII BalI BamHI BanII BbrPI BbvCI BcgI BciVI BclI BcnI BfaI BfrI BglI BglII BlnI BsaBI BsaWI BseAI BseRI BsiWI BsmBI BsmI BsoBI Bsp1407I BspDI BspEI BspHI BspLU11I BspMI BsrBI BsrDI BsrFI BsrGI BssSI Bst1107I BstAPI BstBI BstZ17I Bsu36I Cfr10I ClaI CpoI DraI DraII DrdI EaeI EagI Eam1105I EarI EclXI Eco47III Eco52I Eco81I EcoO109I EcoRI EcoRV EcoT14I EcoT22I FbaI FseI FspI HapII HincII HindII HindIII HpaI HpaII KpnI Ksp632I KspI MaeI MamI MfeI MluI MluNI MroI MscI MspA1I MspI MunI NaeI NciI NcoI NdeI NgoMIV NheI NotI NruI NsiI NspI NspV PacI PaeR7I PflFI PflMI PinAI PmaCI PmeI PmlI PpuMI PshAI PshBI Psp1406I PvuI PvuII RcaI RsaI RsrII SacI SacII SalI SapI SbfI ScaI SfaNI SfiI SfuI SgrAI SmaI SmlI SnaBI SpeI SphI Sse8387I SspBI SspI StuI StyI SwaI TfiI Tsp509I TspRI Tth111I Van91I XbaI XcmI XhoI XmaCI XmaI XmnI ################################### # ONE CUT ENZYMES ( 55 enzymes) # ################################### AcyI 253 GGCGCC G[GA]^CG[CT]C AlwI 359 GGATC GGATC(4/5) AspHI 211 GTGCTC G[AT]GC[AT]^C AvaII 290 GGTCC G^G[AT]CC BanI 253 GGCGCC G^G[CT][GA]CC BbeI 253 GGCGCC GGCGC^C BbsI 435 GAAGAC GAAGAC(2/6) BlpI 213 GCTCAGC GC^T[ACGT]AGC BmyI 211 GTGCTC G[AGT]GC[ACT]^C BpmI 226 CTGGAG CTGGAG(16/14) Bpu1102I 213 GCTCAGC GC^T[ACGT]AGC BpuAI 435 GAAGAC GAAGAC(2/6) BsaAI 238 TACGTG [CT]AC^GT[GA] BsaHI 253 GGCGCC G[GA]^CG[CT]C BsaI 136 GAGACC GGTCTC(1/5) BsgI 91 CTGCAC GTGCAG(16/14) BsiEI 33 CGACCG CG[GA][CT]^CG BsiHKAI 211 GTGCTC G[AT]GC[AT]^C BsmAI 136 GAGAC GTCTC(1/5) BsmFI 354 GGGAC GGGAC(10/14) Bsp1286I 211 GTGCTC G[AGT]GC[ACT]^C BssHII 22 GCGCGC G^CGCGC BstEII 459 GGTCACC G^GT[ACGT]ACC BstF5I 153 CATCC GGATG(2/0) BstPI 459 GGTCACC G^GT[ACGT]ACC BstXI 257 CCATCTGCATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG BstYI 359 GGATCT [GA]^GATC[CT] CelII 213 GCTCAGC GC^T[ACGT]AGC DpnII 360 GATC ^GATC DraIII 94 CACCAGGTG CAC[ACGT][ACGT][ACGT]^GTG EcoNI 339 CCTGGTCAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG EcoO65I 459 GGTCACC G^GT[ACGT]ACC FokI 153 CATCC GGATG(9/13) HgaI 438 GACGC GACGC(5/10) Hin1I 253 GGCGCC G[GA]^CG[CT]C HinfI 109 GACTC G^A[ACGT]TC KasI 253 GGCGCC G^GCGCC MaeIII 460 GTCAC ^GT[ACGT]AC MboI 360 GATC ^GATC MboII 435 GAAGA GAAGA(8/7) MflI 359 GGATCT [GA]^GATC[CT] MseI 422 TTAA T^TAA NarI 253 GGCGCC GG^CGCC NdeII 360 GATC ^GATC NlaIV 253 GGCGCC GG[ACGT]^[ACGT]CC PleI 109 GACTC GAGTC(4/5) PstI 10 CTGCAG CTGCA^G Sau3AI 360 GATC ^GATC SexAI 95 ACCAGGT A^CC[AT]GGT SfoI 253 GGCGCC GGC^GCC TaqI 74 TCGA T^CGA Tru9I 422 TTAA T^TAA Tsp45I 460 GTCAC ^GT[GC]AC TthHB8I 74 TCGA T^CGA XhoII 359 GGATCT [GA]^GATC[CT] ################################### # TWO CUT ENZYMES ( 10 enzymes) # ################################### BsrI 77 ACTGG ACTGG(1/-1) 324 CCAGT Cfr13I 290 GGTCC G^G[ACGT]CC 348 GGGCC DdeI 28 CTTAG C^T[ACGT]AG 214 CTCAG DsaI 47 CCGTGG C^C[GA][CT]GG 305 CCGTGG HaeII 253 GGCGCC [GA]GCGC^[CT] 295 AGCGCC HaeIII 312 GGCC GG^CC 349 GGCC MaeII 83 ACGT A^CGT 239 ACGT MnlI 165 CCTC CCTC(7/6) 310 GAGG Sau96I 290 GGTCC G^G[ACGT]CC 348 GGGCC SfcI 10 CTGCAG C^T[GA][CT]AG 363 CTGTAG ######################################### # 3 OR MORE CUT ENZYMES ( 26 enzymes) # ######################################### AccII AciI AluI BbvI BsaJI BsiYI BslI BssKI BstNI BstUI Cac8I CfoI EcoRII FauI Fnu4HI HhaI HinP1I HphI ItaI MslI MvaI MvnI MwoI NlaIII ScrFI TseI