(Linear) (Six-base) MAPSORT of: KIEE3032.seq  Check: 4049  from: 1  to: 483



KIEE3032.orf

 With 182 enzymes: SgfI * 

                        July 3, 2012 17:20  ..

AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     378     431     483 
   Size:        378      53      52

  Fragments arranged by size: 

                 378      53      52
 
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0     286     483 
   Size:        286     197
 
BanI G'GyrC_C

Cuts at:      0     253     483 
   Size:        253     230
 
BbeI G_GCGC'C

Cuts at:      0     257     483 
   Size:        257     226
 
BbsI GAAGACnn'nnnn_

Cuts at:      0     442     483 
   Size:        442      41
 
BlpI GC'TnA_GC

Cuts at:      0     214     483 
   Size:        214     269
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     247     483 
   Size:        247     236
 
Bpu10I CC'TnA_GC

Cuts at:      0      28     483 
   Size:         28     455
 
BsaI GGTCTCn'nnnn_

Cuts at:      0     130     483 
   Size:        130     353
 
BsaAI yAC'GTr

Cuts at:      0     240     483 
   Size:        240     243
 
BsaHI Gr'CG_yC

Cuts at:      0     254     483 
   Size:        254     229
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      76     483 
   Size:         76     407
 
BsiEI CG_ry'CG

Cuts at:      0      36     483 
   Size:         36     447
 
BsiHKAI G_wGCw'C

Cuts at:      0     215     483 
   Size:        215     268
 
Bsp1286I G_dGCh'C

Cuts at:      0     215     483 
   Size:        215     268
 
BssHII G'CGCG_C

Cuts at:      0      22     483 
   Size:         22     461
 
BstEII G'GTnAC_C

Cuts at:      0     459     483 
   Size:        459      24
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0     264     483 
   Size:        264     219
 
BstYI r'GATC_y

Cuts at:      0     359     483 
   Size:        359     124
 
BtgI C'CryG_G

Cuts at:      0      47     305     483 
   Size:         47     258     178

  Fragments arranged by size: 

                 258     178      47
 
BtgZI GCGATGnnnnnnnnnn'nnnn_

Cuts at:      0       3     483 
   Size:          3     480
 
DraIII CAC_nnn'GTG

Cuts at:      0      99     483 
   Size:         99     384
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     247     378     431     483 
   Size:        247     131      53      52

  Fragments arranged by size: 

                 247     131      53      52
 
EcoNI CCTnn'n_nnAGG

Cuts at:      0     343     483 
   Size:        343     140
 
HaeII r_GCGC'y

Cuts at:      0     257     299     483 
   Size:        257      42     184

  Fragments arranged by size: 

                 257     184      42
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0      94     126     483 
   Size:         94      32     357

  Fragments arranged by size: 

                 357      94      32
 
KasI G'GCGC_C

Cuts at:      0     253     483 
   Size:        253     230
 
MslI CAynn'nnrTG

Cuts at:      0      46     208     262     483 
   Size:         46     162      54     221

  Fragments arranged by size: 

                 221     162      54      46
 
NarI GG'CG_CC

Cuts at:      0     254     483 
   Size:        254     229
 
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0      55      87     483 
   Size:         55      32     396

  Fragments arranged by size: 

                 396      55      32
 
PstI C_TGCA'G

Cuts at:      0      14     483 
   Size:         14     469
 
SexAI A'CCwGG_T

Cuts at:      0      95     483 
   Size:         95     388
 
SfcI C'TryA_G

Cuts at:      0      10     363     483 
   Size:         10     353     120

  Fragments arranged by size: 

                 353     120      10
 
SfoI GGC'GCC

Cuts at:      0     255     483 
   Size:        255     228
 

 Enzymes that do cut:

     AcuI     AlfI     BanI     BbeI     BbsI     BlpI     BpmI   Bpu10I
     BsaI    BsaAI    BsaHI     BsgI    BsiEI  BsiHKAI Bsp1286I   BssHII
   BstEII    BstXI    BstYI     BtgI    BtgZI   DraIII  Eco57MI    EcoNI
    HaeII    Hin4I     KasI     MslI     NarI     PpiI     PstI    SexAI
     SfcI     SfoI

 Enzymes that do not cut: 

     AarI    AatII     AccI   Acc65I     AclI     AfeI    AflII   AflIII
     AgeI     AhdI     AleI     AloI    AlwNI     ApaI    ApaLI     ApoI
     AscI     AseI    AsiSI     AvaI    AvrII     BaeI    BamHI    BanII
    BbvCI     BcgI    BciVI     BclI    BfrBI     BglI    BglII Bme1580I
    BmgBI     BmrI     BmtI     BplI    BpuEI    BsaBI    BsaWI    BsaXI
    BseRI    BseYI    BsiWI     BsmI    BsmBI    BspEI    BspHI    BspMI
    BsrBI    BsrDI    BsrFI    BsrGI    BssSI   BstAPI    BstBI  BstZ17I
   Bsu36I     BtsI     ClaI    CspCI     DraI     DrdI     EaeI     EagI
     EarI     EciI  EcoICRI EcoO109I    EcoRI    EcoRV     FalI     FseI
     FspI    FspAI   HincII  HindIII     HpaI     KpnI     MfeI     MluI
     MmeI     MscI   MspA1I     NaeI     NcoI     NdeI   NgoMIV     NheI
     NotI     NruI     NsiI     NspI     PacI     PasI     PciI    PflMI
     PfoI     PmeI     PmlI    PpuMI    PshAI     PsiI   PspOMI    PspXI
     PsrI     PvuI    PvuII    RsrII     SacI    SacII     SalI    SanDI
     SapI     SbfI     ScaI     SfiI     SgfI    SgrAI     SmaI     SmlI
    SnaBI     SpeI     SphI     SrfI     SspI     StuI     StyI     SwaI
    TaqII     TatI     TstI  Tth111I     XbaI     XcmI     XhoI     XmaI
     XmnI     ZraI