################################## # NO CUT ENZYMES ( 99 enzymes) # ################################## AatII Acc65I AclI AflII AflIII AgeI AhdI Alw44I AlwNI ApaLI AscI AseI AsnI Asp718I AspEI AspHI AviII AvrII BalI BbrPI BbvCI BciVI BclI BfrI BlnI BlpI Bpu1102I BsaAI BsaI BsgI BsiHKAI BsiWI BsmI Bsp1407I BspDI BspLU11I BsrFI BsrGI BssHII BssSI Bst1107I BstAPI BstBI BstEII BstPI BstZ17I CelII Cfr10I ClaI CpoI DraIII DrdI Eam1105I EcoO65I EcoT22I FbaI FseI FspI HpaI KpnI MluI MluNI MscI NaeI NdeI NgoMIV NheI NruI NsiI NspV PacI PaeR7I PflMI PinAI PmaCI PmeI PmlI PshAI PshBI Psp1406I PvuI PvuII RsrII SacI SalI SapI SbfI ScaI SexAI SfuI SgrAI SnaBI SpeI SphI Sse8387I SspBI SwaI Van91I XhoI ################################### # ONE CUT ENZYMES ( 46 enzymes) # ################################### AccI 1076 GTAGAC GT^[AC][GT]AC AccIII 17 TCCGGA T^CCGGA AocI 535 CCTCAGG CC^T[ACGT]AGG Aor51HI 1994 AGCGCT AGC^GCT Asp700I 1648 GAAGAACTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC AspI 2030 GACAGGGTC GAC[ACGT]^[ACGT][ACGT]GTC BamHI 1809 GGATCC G^GATCC BbsI 1373 GTCTTC GAAGAC(2/6) BpuAI 1373 GTCTTC GAAGAC(2/6) BsaBI 1573 GATGCCTATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC BsaWI 17 TCCGGA [AT]^CCGG[AT] BseAI 17 TCCGGA T^CCGGA BsmBI 1620 GAGACG CGTCTC(1/5) BspEI 17 TCCGGA T^CCGGA BspHI 1581 TCATGA T^CATGA BspMI 912 ACCTGC ACCTGC(4/8) BsrDI 1849 GCAATG GCAATG(2/0) BstXI 48 CCACAGGCGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG Bsu36I 535 CCTCAGG CC^T[ACGT]AGG DraI 832 TTTAAA TTT^AAA Eco47III 1994 AGCGCT AGC^GCT Eco81I 535 CCTCAGG CC^T[ACGT]AGG EcoNI 1897 CCTCTAGGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG EcoRI 934 GAATTC G^AATTC EcoRV 919 GATATC GAT^ATC EcoT14I 137 CCATGG C^C[AT][AT]GG HgaI 1640 GACGC GACGC(5/10) HincII 1742 GTCAAC GT[CT]^[GA]AC HindII 1742 GTCAAC GT[CT]^[GA]AC HindIII 1012 AAGCTT A^AGCTT MamI 1573 GATGCCTATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC MfeI 1690 CAATTG C^AATTG MroI 17 TCCGGA T^CCGGA MunI 1690 CAATTG C^AATTG NcoI 137 CCATGG C^CATGG PflFI 2030 GACAGGGTC GAC[ACGT]^[ACGT][ACGT]GTC PstI 464 CTGCAG CTGCA^G RcaI 1581 TCATGA T^CATGA SmlI 852 CTTGAG C^T[CT][GA]AG SspI 1466 AATATT AAT^ATT StuI 859 AGGCCT AGG^CCT StyI 137 CCATGG C^C[AT][AT]GG TspRI 1293 CAGTG CA[GC]TG[ACGT][ACGT]^ Tth111I 2030 GACAGGGTC GAC[ACGT]^[ACGT][ACGT]GTC XbaI 1021 TCTAGA T^CTAGA XmnI 1648 GAAGAACTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC ################################### # TWO CUT ENZYMES ( 22 enzymes) # ################################### AcyI 489 GGCGCC G[GA]^CG[CT]C 1769 GGCGCC BanI 489 GGCGCC G^G[CT][GA]CC 1769 GGCGCC BbeI 489 GGCGCC GGCGC^C 1769 GGCGCC BcgI 466 GCAGTCACCTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10) 992 GCAAAGGATTCG BsaHI 489 GGCGCC G[GA]^CG[CT]C 1769 GGCGCC BsiEI 69 CGGCCG CG[GA][CT]^CG 795 CGGCCG BsrBI 350 CCGCTC CCGCTC(-3/-3) 159 GAGCGG BsrI 1053 ACTGG ACTGG(1/-1) 1330 CCAGT EagI 69 CGGCCG C^GGCCG 795 CGGCCG EclXI 69 CGGCCG C^GGCCG 795 CGGCCG Eco52I 69 CGGCCG C^GGCCG 795 CGGCCG Hin1I 489 GGCGCC G[GA]^CG[CT]C 1769 GGCGCC KasI 489 GGCGCC G^GCGCC 1769 GGCGCC NarI 489 GGCGCC GG^CGCC 1769 GGCGCC NotI 68 GCGGCCGC GC^GGCCGC 794 GCGGCCGC SfcI 464 CTGCAG C^T[GA][CT]AG 1229 CTACAG SfiI 70 GGCCGCGGCGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC 154 GGCCAGAGCGGCC SfoI 489 GGCGCC GGC^GCC 1769 GGCGCC TaqI 705 TCGA T^CGA 1086 TCGA Tsp45I 469 GTCAC ^GT[GC]AC 1979 GTGAC TthHB8I 705 TCGA T^CGA 1086 TCGA XcmI 1043 CCAAAGCCGAACTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG 1148 CCAATGAACTGTTGG ######################################### # 3 OR MORE CUT ENZYMES ( 91 enzymes) # ######################################### AccII AciI AcsI AfaI AluI AlwI ApaI ApoI AvaI AvaII BanII BbvI BcnI BfaI BglI BglII BmyI BpmI BsaJI BseRI BsiYI BslI BsmAI BsmFI BsoBI Bsp1286I BssKI BstF5I BstNI BstUI BstYI Cac8I CfoI Cfr13I DdeI DpnII DraII DsaI EaeI EarI EcoO109I EcoRII FauI Fnu4HI FokI HaeII HaeIII HapII HhaI HinP1I HinfI HpaII HphI ItaI Ksp632I KspI MaeI MaeII MaeIII MboI MboII MflI MnlI MseI MslI MspA1I MspI MvaI MvnI MwoI NciI NdeII NlaIII NlaIV NspI PleI PpuMI RsaI SacII Sau3AI Sau96I ScrFI SfaNI SmaI TfiI Tru9I TseI Tsp509I XhoII XmaCI XmaI