(Circular) (Six-base) MAPSORT of: pFN21AB9976.seq Check: 2709 from: 1 to: 6826 pFN21AB9976 6826 bp With 182 enzymes: SgfI * July 6, 2012 17:34 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 4636 278 Size: 53 83 186 936 3100 2468 Fragments arranged by size: 3100 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3082 4169 1937 Size: 1145 1087 4594 Fragments arranged by size: 4594 1145 1087 Acc65I G'GTAC_C Cuts at: 4147 4147 Size: 6826 AclI AA'CG_TT Cuts at: 1563 4953 5326 1563 Size: 3390 373 3063 Fragments arranged by size: 3390 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 4889 6273 6541 1632 Size: 3257 1384 268 1917 Fragments arranged by size: 3257 1917 1384 268 AfeI AGC'GCT Cuts at: 4001 4001 Size: 6826 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 6629 AflIII A'CryG_T Cuts at: 1203 5725 6702 1203 Size: 4522 977 1327 Fragments arranged by size: 4522 1327 977 AgeI A'CCGG_T Cuts at: 5657 5657 Size: 6826 AhdI GACnn_n'nnGTC Cuts at: 5555 5555 Size: 6826 AleI CACnn'nnGTG Cuts at: 1166 2058 1166 Size: 892 5934 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 4032 1184 Size: 2848 3978 AlwNI CAG_nnn'CTG Cuts at: 4569 6141 4569 Size: 1572 5254 ApaI G_GGCC'C Cuts at: 2428 2585 2609 2781 2796 2428 Size: 157 24 172 15 6458 Fragments arranged by size: 6458 172 157 24 15 ApaLI G'TGCA_C Cuts at: 4883 6039 4883 Size: 1156 5670 ApoI r'AATT_y Cuts at: 1493 2939 3254 3893 4135 4361 4513 6622 1493 Size: 1446 315 639 242 226 152 2109 1697 Fragments arranged by size: 2109 1697 1446 639 315 242 226 152 AseI AT'TA_AT Cuts at: 160 1032 5380 160 Size: 872 4348 1606 Fragments arranged by size: 4348 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6826 AvaI C'yCGr_G Cuts at: 1870 1943 2010 2155 2419 2489 2578 2584 2668 Size: 73 67 145 264 70 89 6 84 Cuts at: 2668 4151 1870 Size: 1483 4545 Fragments arranged by size: 4545 1483 264 145 89 84 73 70 67 6 BamHI G'GATC_C Cuts at: 3814 4156 4523 3814 Size: 342 367 6117 Fragments arranged by size: 6117 367 342 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2494 3774 4147 5602 618 Size: 325 217 627 707 1280 373 1455 1842 Fragments arranged by size: 1842 1455 1280 707 627 373 325 217 BanII G_rGCy'C Cuts at: 1391 2428 2585 2609 2727 2781 2796 4145 1391 Size: 1037 157 24 118 54 15 1349 4072 Fragments arranged by size: 4072 1349 1037 157 118 54 24 15 BbeI G_GCGC'C Cuts at: 2498 3778 2498 Size: 1280 5546 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 3371 928 Size: 971 1472 4383 Fragments arranged by size: 4383 1472 971 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 2460 2494 2986 3020 5017 5051 1903 Size: 34 523 34 492 34 1997 34 3678 Fragments arranged by size: 3678 1997 523 492 34 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 4718 5928 4718 Size: 1210 5616 BclI T'GATC_A Cuts at: 1548 1548 Size: 6826 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2082 2166 2647 Size: 107 122 71 631 605 410 84 481 Cuts at: 2647 2791 3804 5437 136 Size: 144 1013 1633 1525 Fragments arranged by size: 1633 1525 1013 631 605 481 410 144 122 107 84 71 BglII A'GATC_T Cuts at: 2817 3230 3616 6821 2817 Size: 413 386 3205 2822 Fragments arranged by size: 3205 2822 413 386 BlpI GC'TnA_GC Cuts at: 4243 4243 Size: 6826 Bme1580I G_kGCm'C Cuts at: 1790 2428 2585 2609 2781 2796 4887 6043 1790 Size: 638 157 24 172 15 2091 1156 2573 Fragments arranged by size: 2573 2091 1156 638 172 157 24 15 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 6826 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 4557 5515 442 Size: 416 53 83 399 24 3140 958 1753 Fragments arranged by size: 3140 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6826 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2836 2836 Size: 6826 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2371 2889 4050 5486 1239 Size: 1132 518 1161 1436 2579 Fragments arranged by size: 2579 1436 1161 1132 518 Bpu10I CC'TnA_GC Cuts at: 2172 2340 2598 2172 Size: 168 258 6400 Fragments arranged by size: 6400 258 168 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2878 4951 5816 6114 2878 Size: 2073 865 298 3590 Fragments arranged by size: 3590 2073 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 5489 882 Size: 617 3990 2219 Fragments arranged by size: 3990 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 4617 493 Size: 711 3413 2702 Fragments arranged by size: 3413 2702 711 BsaBI GATnn'nnATC Cuts at: 3582 6820 3582 Size: 3238 3588 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2495 3775 4633 5015 Size: 53 83 186 936 962 1280 858 382 Cuts at: 5015 5650 275 Size: 635 1451 Fragments arranged by size: 1451 1280 962 936 858 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 2022 5259 5657 5931 6078 6489 2022 Size: 3237 398 274 147 411 2359 Fragments arranged by size: 3237 2359 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2521 2551 3310 3340 1178 Size: 30 165 30 1118 30 759 30 4664 Fragments arranged by size: 4664 1118 759 165 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2079 2529 2825 2875 3318 3426 3722 3864 Size: 893 450 296 50 443 108 296 142 Cuts at: 3864 1186 Size: 4148 Fragments arranged by size: 4148 893 450 443 296 296 142 108 50 BseYI C'CCAG_C Cuts at: 3447 6029 6525 3447 Size: 2582 496 3748 Fragments arranged by size: 3748 2582 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 1163 Size: 573 6253 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2077 2803 4276 5037 6065 1540 Size: 275 186 76 726 1473 761 1028 2301 Fragments arranged by size: 2301 1473 1028 761 726 275 186 76 BsiHKAI G_wGCw'C Cuts at: 4887 4972 6043 4887 Size: 85 1071 5670 Fragments arranged by size: 5670 1071 85 BsmI GAATG_Cn' Cuts at: 4337 4430 4337 Size: 93 6733 BsmBI CGTCTCn'nnnn_ Cuts at: 3619 3619 Size: 6826 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2428 2585 2609 2727 2781 2796 4145 Size: 399 638 157 24 118 54 15 1349 Cuts at: 4145 4887 4972 6043 1391 Size: 742 85 1071 2174 Fragments arranged by size: 2174 1349 1071 742 638 399 157 118 85 54 24 15 BspEI T'CCGG_A Cuts at: 2022 6489 2022 Size: 4467 2359 BspHI T'CATG_A Cuts at: 3586 4715 6445 3586 Size: 1129 1730 3967 Fragments arranged by size: 3967 1730 1129 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2926 4181 844 Size: 2082 1255 3489 Fragments arranged by size: 3489 2082 1255 BsrBI CCG'CTC Cuts at: 1394 1823 2166 2357 4713 1394 Size: 429 343 191 2356 3507 Fragments arranged by size: 3507 2356 429 343 191 BsrDI GCAATG_nn' Cuts at: 65 1248 3861 5321 5495 65 Size: 1183 2613 1460 174 1396 Fragments arranged by size: 2613 1460 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 5470 5657 1700 Size: 110 3660 187 2869 Fragments arranged by size: 3660 2869 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 6826 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6826 BssSI C'ACGA_G Cuts at: 4886 5898 4886 Size: 1012 5814 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 6826 BstBI TT'CG_AA Cuts at: 5630 5630 Size: 6826 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 6826 BstXI CCAn_nnnn'nTGG Cuts at: 2060 2060 Size: 6826 BstYI r'GATC_y Cuts at: 1972 2817 3230 3616 3814 3868 4156 4523 4908 Size: 845 413 386 198 54 288 367 385 Cuts at: 4908 4925 6377 6463 6475 6821 1972 Size: 17 1452 86 12 346 1977 Fragments arranged by size: 1977 1452 845 413 386 385 367 346 288 198 86 54 17 12 Bsu36I CC'TnA_GG Cuts at: 2541 2541 Size: 6826 BtgI C'CryG_G Cuts at: 513 1065 2077 2142 2568 2728 3818 513 Size: 552 1012 65 426 160 1090 3521 Fragments arranged by size: 3521 1090 1012 552 426 160 65 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 2905 3496 487 Size: 683 56 1679 591 3817 Fragments arranged by size: 3817 1679 683 591 56 BtsI GCAGTG_nn' Cuts at: 3304 4342 5136 5156 3304 Size: 1038 794 20 4974 Fragments arranged by size: 4974 1038 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6826 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6791 DraI TTT'AAA Cuts at: 2839 4130 2839 Size: 1291 5535 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5833 809 Size: 507 4517 1802 Fragments arranged by size: 4517 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2074 2689 2800 Size: 54 1425 50 275 18 244 615 111 Cuts at: 2800 4273 5162 6791 8 Size: 1473 889 1629 43 Fragments arranged by size: 1629 1473 1425 889 615 275 244 111 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 2074 2800 4273 1537 Size: 275 262 726 1473 4090 Fragments arranged by size: 4090 1473 726 275 262 EarI CTCTTCn'nnn_ Cuts at: 1353 2326 3718 3726 4086 4756 1353 Size: 973 1392 8 360 670 3423 Fragments arranged by size: 3423 1392 973 670 360 8 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2094 2553 3861 5413 5787 5933 Size: 1064 138 505 459 1308 1552 374 146 Cuts at: 5933 387 Size: 1280 Fragments arranged by size: 1552 1308 1280 1064 505 459 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2371 2889 4050 4889 5486 6273 6541 Size: 393 739 518 1161 839 597 787 268 Cuts at: 6541 1239 Size: 1524 Fragments arranged by size: 1524 1161 839 787 739 597 518 393 268 EcoNI CCTnn'n_nnAGG Cuts at: 3906 3906 Size: 6826 EcoO109I rG'GnC_Cy Cuts at: 2147 2345 2424 2425 2524 2594 2605 2777 2778 Size: 198 79 1 99 70 11 172 1 Cuts at: 2778 4022 4040 4055 2147 Size: 1244 18 15 4918 Fragments arranged by size: 4918 1244 198 172 99 79 70 18 15 11 1 1 EcoRI G'AATT_C Cuts at: 2939 4135 4513 2939 Size: 1196 378 5252 Fragments arranged by size: 5252 1196 378 EcoRV GAT'ATC Cuts at: 2926 2926 Size: 6826 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 3395 3395 Size: 6826 FspI TGC'GCA Cuts at: 5332 5332 Size: 6826 HaeII r_GCGC'y Cuts at: 1678 2498 3778 4003 5973 1678 Size: 820 1280 225 1970 2531 Fragments arranged by size: 2531 1970 1280 820 225 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 3310 3313 3342 3345 3730 Size: 32 135 32 1738 3 29 3 385 Cuts at: 3730 3762 5467 5499 5541 5573 1373 Size: 32 1705 32 42 32 2626 Fragments arranged by size: 2626 1738 1705 385 135 42 32 32 32 32 32 29 3 3 HincII GTy'rAC Cuts at: 669 1657 1938 3749 4170 4416 669 Size: 988 281 1811 421 246 3079 Fragments arranged by size: 3079 1811 988 421 281 246 HindIII A'AGCT_T Cuts at: 748 3017 748 Size: 2269 4557 HpaI GTT'AAC Cuts at: 4416 4416 Size: 6826 KasI G'GCGC_C Cuts at: 2494 3774 2494 Size: 1280 5546 KpnI G_GTAC'C Cuts at: 4151 4151 Size: 6826 MfeI C'AATT_G Cuts at: 3695 4425 3695 Size: 730 6096 MluI A'CGCG_T Cuts at: 6702 6702 Size: 6826 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2480 3144 5940 6124 1305 Size: 1175 664 2796 184 2007 Fragments arranged by size: 2796 2007 1175 664 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 5004 Fragments arranged by size: 5004 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2058 2890 3836 4784 5143 5302 518 Size: 648 892 832 946 948 359 159 2042 Fragments arranged by size: 2042 948 946 892 832 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2079 2520 2570 2730 4242 4919 6067 6312 Size: 473 441 50 160 1512 677 1148 245 Cuts at: 6312 1606 Size: 2120 Fragments arranged by size: 2120 1512 1148 677 473 441 245 160 50 NarI GG'CG_CC Cuts at: 2495 3775 2495 Size: 1280 5546 NcoI C'CATG_G Cuts at: 513 1065 2142 513 Size: 552 1077 5197 Fragments arranged by size: 5197 1077 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 6826 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6826 NotI GC'GGCC_GC Cuts at: 2074 2800 4273 2074 Size: 726 1473 4627 Fragments arranged by size: 4627 1473 726 NspI r_CATG'y Cuts at: 1132 1229 3113 3835 3991 4184 5729 1132 Size: 97 1884 722 156 193 1545 2229 Fragments arranged by size: 2229 1884 1545 722 193 156 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 6826 PciI A'CATG_T Cuts at: 5725 5725 Size: 6826 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 6826 PfoI T'CCnGG_A Cuts at: 2359 2359 Size: 6826 PmeI GTTT'AAAC Cuts at: 4130 4130 Size: 6826 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4895 4927 6408 6440 4895 Size: 32 1481 32 5281 Fragments arranged by size: 5281 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2147 2345 2524 4040 2147 Size: 198 179 1516 4933 Fragments arranged by size: 4933 1516 198 179 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6826 PsiI TTA'TAA Cuts at: 4396 4396 Size: 6826 PspOMI G'GGCC_C Cuts at: 2424 2581 2605 2777 2792 2424 Size: 157 24 172 15 6458 Fragments arranged by size: 6458 172 157 24 15 PstI C_TGCA'G Cuts at: 830 2473 4178 830 Size: 1643 1705 3478 Fragments arranged by size: 3478 1705 1643 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6826 SacII CC_GC'GG Cuts at: 2080 2571 2731 2080 Size: 491 160 6175 Fragments arranged by size: 6175 491 160 SalI G'TCGA_C Cuts at: 1936 4168 1936 Size: 2232 4594 SanDI GG'GwC_CC Cuts at: 2345 2524 2345 Size: 179 6647 SbfI CC_TGCA'GG Cuts at: 4178 4178 Size: 6826 ScaI AGT'ACT Cuts at: 5074 6786 5074 Size: 1712 5114 SfcI C'TryA_G Cuts at: 826 1045 2469 3234 4174 5309 5990 6181 826 Size: 219 1424 765 940 1135 681 191 1471 Fragments arranged by size: 1471 1424 1135 940 765 681 219 191 SfiI GGCCn_nnn'nGGCC Cuts at: 2082 2166 2082 Size: 84 6742 SfoI GGC'GCC Cuts at: 2496 3776 2496 Size: 1280 5546 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6826 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6826 SmaI CCC'GGG Cuts at: 1872 2157 2491 2580 2586 2670 4153 1872 Size: 285 334 89 6 84 1483 4545 Fragments arranged by size: 4545 1483 334 285 89 84 6 SmlI C'TyrA_G Cuts at: 820 1017 1943 2857 4930 5831 6093 820 Size: 197 926 914 2073 901 262 1553 Fragments arranged by size: 2073 1553 926 914 901 262 197 SnaBI TAC'GTA Cuts at: 493 4617 493 Size: 4124 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6826 SphI G_CATG'C Cuts at: 1132 4184 1132 Size: 3052 3774 SrfI GCCC'GGGC Cuts at: 2157 2670 2157 Size: 513 6313 SspI AAT'ATT Cuts at: 5 52 3473 4750 5 Size: 47 3421 1277 2081 Fragments arranged by size: 3421 2081 1277 47 StuI AGG'CCT Cuts at: 1517 2866 1517 Size: 1349 5477 StyI C'CwwG_G Cuts at: 513 1065 2142 4265 513 Size: 552 1077 2123 3074 Fragments arranged by size: 3074 2123 1077 552 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2518 3085 3830 4019 4853 4870 5023 Size: 1255 938 567 745 189 834 17 153 Cuts at: 5023 5208 5653 325 Size: 185 445 1498 Fragments arranged by size: 1498 1255 938 834 745 567 445 189 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 5072 6784 96 Size: 274 80 33 51 1443 3095 1712 138 Fragments arranged by size: 3095 1712 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 6786 6818 6786 Size: 32 6794 Tth111I GACn'n_nGTC Cuts at: 1316 4038 1316 Size: 2722 4104 XbaI T'CTAG_A Cuts at: 3026 4162 3026 Size: 1136 5690 XcmI CCAnnnn_n'nnnnTGG Cuts at: 3055 3160 3055 Size: 105 6721 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6826 XmaI C'CCGG_G Cuts at: 1870 2155 2489 2578 2584 2668 4151 1870 Size: 285 334 89 6 84 1483 4545 Fragments arranged by size: 4545 1483 334 285 89 84 6 XmnI GAAnn'nnTTC Cuts at: 3657 4955 3657 Size: 1298 5528 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 4634 276 Size: 53 83 186 936 3100 2468 Fragments arranged by size: 3100 2468 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AfeI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoNI EcoO109I EcoRI EcoRV FalI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NarI NcoI NdeI NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI SacII SalI SanDI SbfI ScaI SfcI SfiI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SrfI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AloI AscI AvrII BaeI BbvCI BfrBI BsiWI BstZ17I DraIII EcoICRI FseI FspAI NaeI NgoMIV NruI NsiI PacI PmlI PspXI PsrI PvuII RsrII SacI SapI SexAI SwaI