(Linear) (Six-base) MAPSORT of: KIBB9976.seq Check: 4078 from: 1 to: 2121 KIBB9976.orf With 182 enzymes: SgfI * July 3, 2012 16:40 .. AccI GT'mk_AC Cuts at: 0 1077 2121 Size: 1077 1044 AfeI AGC'GCT Cuts at: 0 1996 2121 Size: 1996 125 AleI CACnn'nnGTG Cuts at: 0 53 2121 Size: 53 2068 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 0 2027 2121 Size: 2027 94 ApaI G_GGCC'C Cuts at: 0 423 580 604 776 791 2121 Size: 423 157 24 172 15 1330 Fragments arranged by size: 1330 423 172 157 24 15 ApoI r'AATT_y Cuts at: 0 934 1249 1888 2121 Size: 934 315 639 233 Fragments arranged by size: 934 639 315 233 AvaI C'yCGr_G Cuts at: 0 5 150 414 484 573 579 663 2121 Size: 5 145 264 70 89 6 84 1458 Fragments arranged by size: 1458 264 145 89 84 70 6 5 BamHI G'GATC_C Cuts at: 0 1809 2121 Size: 1809 312 BanI G'GyrC_C Cuts at: 0 489 1769 2121 Size: 489 1280 352 Fragments arranged by size: 1280 489 352 BanII G_rGCy'C Cuts at: 0 423 580 604 722 776 791 2121 Size: 423 157 24 118 54 15 1330 Fragments arranged by size: 1330 423 157 118 54 24 15 BbeI G_GCGC'C Cuts at: 0 493 1773 2121 Size: 493 1280 348 Fragments arranged by size: 1280 493 348 BbsI GAAGACnn'nnnn_ Cuts at: 0 1366 2121 Size: 1366 755 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 0 455 489 981 1015 2121 Size: 455 34 492 34 1106 Fragments arranged by size: 1106 492 455 34 34 BglI GCCn_nnn'nGGC Cuts at: 0 77 161 642 786 1799 2121 Size: 77 84 481 144 1013 322 Fragments arranged by size: 1013 481 322 144 84 77 BglII A'GATC_T Cuts at: 0 812 1225 1611 2121 Size: 812 413 386 510 Fragments arranged by size: 812 510 413 386 Bme1580I G_kGCm'C Cuts at: 0 423 580 604 776 791 2121 Size: 423 157 24 172 15 1330 Fragments arranged by size: 1330 423 172 157 24 15 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 0 831 2121 Size: 831 1290 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 366 884 2045 2121 Size: 366 518 1161 76 Fragments arranged by size: 1161 518 366 76 Bpu10I CC'TnA_GC Cuts at: 0 167 335 593 2121 Size: 167 168 258 1528 Fragments arranged by size: 1528 258 168 167 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 873 2121 Size: 873 1248 BsaBI GATnn'nnATC Cuts at: 0 1577 2121 Size: 1577 544 BsaHI Gr'CG_yC Cuts at: 0 490 1770 2121 Size: 490 1280 351 Fragments arranged by size: 1280 490 351 BsaWI w'CCGG_w Cuts at: 0 17 2121 Size: 17 2104 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 0 516 546 1305 1335 2121 Size: 516 30 759 30 786 Fragments arranged by size: 786 759 516 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 0 74 524 820 870 1313 1421 1717 1859 Size: 74 450 296 50 443 108 296 142 Cuts at: 1859 2121 Size: 262 Fragments arranged by size: 450 443 296 296 262 142 108 74 50 BseYI C'CCAG_C Cuts at: 0 1442 2121 Size: 1442 679 BsiEI CG_ry'CG Cuts at: 0 72 798 2121 Size: 72 726 1323 Fragments arranged by size: 1323 726 72 BsmBI CGTCTCn'nnnn_ Cuts at: 0 1614 2121 Size: 1614 507 Bsp1286I G_dGCh'C Cuts at: 0 423 580 604 722 776 791 2121 Size: 423 157 24 118 54 15 1330 Fragments arranged by size: 1330 423 157 118 54 24 15 BspEI T'CCGG_A Cuts at: 0 17 2121 Size: 17 2104 BspHI T'CATG_A Cuts at: 0 1581 2121 Size: 1581 540 BspMI ACCTGCnnnn'nnnn_ Cuts at: 0 921 2121 Size: 921 1200 BsrBI CCG'CTC Cuts at: 0 161 352 2121 Size: 161 191 1769 Fragments arranged by size: 1769 191 161 BsrDI GCAATG_nn' Cuts at: 0 1856 2121 Size: 1856 265 BstXI CCAn_nnnn'nTGG Cuts at: 0 55 2121 Size: 55 2066 BstYI r'GATC_y Cuts at: 0 812 1225 1611 1809 1863 2121 Size: 812 413 386 198 54 258 Fragments arranged by size: 812 413 386 258 198 54 Bsu36I CC'TnA_GG Cuts at: 0 536 2121 Size: 536 1585 BtgI C'CryG_G Cuts at: 0 72 137 563 723 1813 2121 Size: 72 65 426 160 1090 308 Fragments arranged by size: 1090 426 308 160 72 65 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 0 900 1491 2121 Size: 900 591 630 Fragments arranged by size: 900 630 591 BtsI GCAGTG_nn' Cuts at: 0 1299 2121 Size: 1299 822 DraI TTT'AAA Cuts at: 0 834 2121 Size: 834 1287 EaeI y'GGCC_r Cuts at: 0 69 684 795 2121 Size: 69 615 111 1326 Fragments arranged by size: 1326 615 111 69 EagI C'GGCC_G Cuts at: 0 69 795 2121 Size: 69 726 1326 Fragments arranged by size: 1326 726 69 EarI CTCTTCn'nnn_ Cuts at: 0 321 1713 1721 2081 2121 Size: 321 1392 8 360 40 Fragments arranged by size: 1392 360 321 40 8 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 0 89 548 1856 2121 Size: 89 459 1308 265 Fragments arranged by size: 1308 459 265 89 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 0 366 884 2045 2121 Size: 366 518 1161 76 Fragments arranged by size: 1161 518 366 76 EcoNI CCTnn'n_nnAGG Cuts at: 0 1901 2121 Size: 1901 220 EcoO109I rG'GnC_Cy Cuts at: 0 142 340 419 420 519 589 600 772 Size: 142 198 79 1 99 70 11 172 Cuts at: 772 773 2017 2035 2050 2121 Size: 1 1244 18 15 71 Fragments arranged by size: 1244 198 172 142 99 79 71 70 18 15 11 1 1 EcoRI G'AATT_C Cuts at: 0 934 2121 Size: 934 1187 EcoRV GAT'ATC Cuts at: 0 921 2121 Size: 921 1200 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 0 1390 2121 Size: 1390 731 HaeII r_GCGC'y Cuts at: 0 493 1773 1998 2121 Size: 493 1280 225 123 Fragments arranged by size: 1280 493 225 123 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 0 1305 1308 1337 1340 1725 1757 2121 Size: 1305 3 29 3 385 32 364 Fragments arranged by size: 1305 385 364 32 29 3 3 HincII GTy'rAC Cuts at: 0 1744 2121 Size: 1744 377 HindIII A'AGCT_T Cuts at: 0 1012 2121 Size: 1012 1109 KasI G'GCGC_C Cuts at: 0 489 1769 2121 Size: 489 1280 352 Fragments arranged by size: 1280 489 352 MfeI C'AATT_G Cuts at: 0 1690 2121 Size: 1690 431 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 0 475 1139 2121 Size: 475 664 982 Fragments arranged by size: 982 664 475 MslI CAynn'nnrTG Cuts at: 0 53 885 1831 2121 Size: 53 832 946 290 Fragments arranged by size: 946 832 290 53 MspA1I CmG'CkG Cuts at: 0 74 515 565 725 2121 Size: 74 441 50 160 1396 Fragments arranged by size: 1396 441 160 74 50 NarI GG'CG_CC Cuts at: 0 490 1770 2121 Size: 490 1280 351 Fragments arranged by size: 1280 490 351 NcoI C'CATG_G Cuts at: 0 137 2121 Size: 137 1984 NotI GC'GGCC_GC Cuts at: 0 69 795 2121 Size: 69 726 1326 Fragments arranged by size: 1326 726 69 NspI r_CATG'y Cuts at: 0 1108 1830 1986 2121 Size: 1108 722 156 135 Fragments arranged by size: 1108 722 156 135 PfoI T'CCnGG_A Cuts at: 0 354 2121 Size: 354 1767 PpuMI rG'GwC_Cy Cuts at: 0 142 340 519 2035 2121 Size: 142 198 179 1516 86 Fragments arranged by size: 1516 198 179 142 86 PspOMI G'GGCC_C Cuts at: 0 419 576 600 772 787 2121 Size: 419 157 24 172 15 1334 Fragments arranged by size: 1334 419 172 157 24 15 PstI C_TGCA'G Cuts at: 0 468 2121 Size: 468 1653 SacII CC_GC'GG Cuts at: 0 75 566 726 2121 Size: 75 491 160 1395 Fragments arranged by size: 1395 491 160 75 SanDI GG'GwC_CC Cuts at: 0 340 519 2121 Size: 340 179 1602 Fragments arranged by size: 1602 340 179 SfcI C'TryA_G Cuts at: 0 464 1229 2121 Size: 464 765 892 Fragments arranged by size: 892 765 464 SfiI GGCCn_nnn'nGGCC Cuts at: 0 77 161 2121 Size: 77 84 1960 Fragments arranged by size: 1960 84 77 SfoI GGC'GCC Cuts at: 0 491 1771 2121 Size: 491 1280 350 Fragments arranged by size: 1280 491 350 SmaI CCC'GGG Cuts at: 0 152 486 575 581 665 2121 Size: 152 334 89 6 84 1456 Fragments arranged by size: 1456 334 152 89 84 6 SmlI C'TyrA_G Cuts at: 0 852 2121 Size: 852 1269 SrfI GCCC'GGGC Cuts at: 0 152 665 2121 Size: 152 513 1456 Fragments arranged by size: 1456 513 152 SspI AAT'ATT Cuts at: 0 1468 2121 Size: 1468 653 StuI AGG'CCT Cuts at: 0 861 2121 Size: 861 1260 StyI C'CwwG_G Cuts at: 0 137 2121 Size: 137 1984 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 0 513 1080 1825 2014 2121 Size: 513 567 745 189 107 Fragments arranged by size: 745 567 513 189 107 Tth111I GACn'n_nGTC Cuts at: 0 2033 2121 Size: 2033 88 XbaI T'CTAG_A Cuts at: 0 1021 2121 Size: 1021 1100 XcmI CCAnnnn_n'nnnnTGG Cuts at: 0 1050 1155 2121 Size: 1050 105 966 Fragments arranged by size: 1050 966 105 XmaI C'CCGG_G Cuts at: 0 150 484 573 579 663 2121 Size: 150 334 89 6 84 1458 Fragments arranged by size: 1458 334 150 89 84 6 XmnI GAAnn'nnTTC Cuts at: 0 1652 2121 Size: 1652 469 Enzymes that do cut: AccI AfeI AleI AlfI ApaI ApoI AvaI BamHI BanI BanII BbeI BbsI BcgI BglI BglII Bme1580I BplI BpmI Bpu10I BpuEI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsiEI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BstXI BstYI Bsu36I BtgI BtgZI BtsI DraI EaeI EagI EarI EciI Eco57MI EcoNI EcoO109I EcoRI EcoRV FalI HaeII Hin4I HincII HindIII KasI MfeI MmeI MslI MspA1I NarI NcoI NotI NspI PfoI PpuMI PspOMI PstI SacII SanDI SfcI SfiI SfoI SmaI SmlI SrfI SspI StuI StyI TaqII Tth111I XbaI XcmI XmaI XmnI Enzymes that do not cut: AarI AatII Acc65I AclI AcuI AflII AflIII AgeI AhdI AloI AlwNI ApaLI AscI AseI AsiSI AvrII BaeI BbvCI BciVI BclI BfrBI BlpI BmgBI BmrI BmtI BsaI BsaAI BsgI BsiHKAI BsiWI BsmI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstZ17I ClaI CspCI DraIII DrdI EcoICRI FseI FspI FspAI HpaI KpnI MluI MscI NaeI NdeI NgoMIV NheI NruI NsiI PacI PasI PciI PflMI PmeI PmlI PpiI PshAI PsiI PspXI PsrI PvuI PvuII RsrII SacI SalI SapI SbfI ScaI SexAI SgfI SgrAI SnaBI SpeI SphI SwaI TatI TstI XhoI ZraI