################################## # NO CUT ENZYMES ( 119 enzymes) # ################################## AatII Acc65I AccIII AclI AflIII AgeI AhdI Alw44I ApaLI AscI AseI AsnI Asp700I Asp718I AspEI AspI AvrII BalI BbrPI BcgI BciVI BfaI BglI BglII BlnI BlpI Bpu1102I BsaAI BsaI BsaWI BseAI BsiEI BsiWI BsmBI BsmI Bsp1407I BspDI BspEI BspHI BspLU11I BsrDI BsrGI BssSI Bst1107I BstBI BstEII BstPI BstXI BstZ17I CelII ClaI CpoI DraI DraIII DrdI EagI Eam1105I EclXI Eco52I EcoNI EcoO65I EcoRI EcoRV EcoT22I FseI HincII HindII HindIII HpaI KpnI KspI MaeI MfeI MluI MluNI MroI MscI MunI NdeI NheI NotI NruI NsiI NspV PacI PaeR7I PflFI PflMI PinAI PmaCI PmeI PmlI PshAI PshBI Psp1406I PvuI RcaI RsrII SacII SalI SapI ScaI SfiI SfuI SgrAI SnaBI SpeI SspBI SspI StuI SwaI TaqI TfiI Tth111I TthHB8I Van91I XbaI XhoI XmnI ################################### # ONE CUT ENZYMES ( 40 enzymes) # ################################### AccI 160 GTCTAC GT^[AC][GT]AC AcsI 1262 AAATTT [GA]^AATT[CT] AfaI 1173 GTAC GT^AC AflII 855 CTTAAG C^TTAAG AocI 108 CCTCAGG CC^T[ACGT]AGG Aor51HI 539 AGCGCT AGC^GCT ApoI 1262 AAATTT [GA]^AATT[CT] AviII 929 TGCGCA TGC^GCA BamHI 885 GGATCC G^GATCC BbvCI 600 GCTGAGG CCTCAGC(-5/-2) BclI 1449 TGATCA T^GATCA BfrI 855 CTTAAG C^TTAAG BsaBI 1447 GATGATCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC BsgI 937 GTGCAG GTGCAG(16/14) BsmAI 639 GAGAC GTCTC(1/5) BsmFI 964 GTCCC GGGAC(10/14) BspMI 926 ACCTGC ACCTGC(4/8) BsrBI 22 GAGCGG CCGCTC(-3/-3) BstAPI 636 GCAGAGACTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC Bsu36I 108 CCTCAGG CC^T[ACGT]AGG EaeI 93 CGGCCA [CT]^GGCC[GA] Eco47III 539 AGCGCT AGC^GCT Eco81I 108 CCTCAGG CC^T[ACGT]AGG FbaI 1449 TGATCA T^GATCA FspI 929 TGCGCA TGC^GCA HphI 1258 GGTGA GGTGA(8/7) MaeII 488 ACGT A^CGT MamI 1447 GATGATCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC MseI 856 TTAA T^TAA MslI 804 CATCCCCATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG NspI 826 GCATGC [GA]CATG^[CT] PpuMI 302 AGGACCT [GA]G^G[AT]CC[CT] RsaI 1173 GTAC GT^AC SacI 850 GAGCTC GAGCT^C SbfI 66 CCTGCAGG CCTGCA^GG SexAI 416 ACCAGGT A^CC[AT]GGT SmlI 855 CTTAAG C^T[CT][GA]AG SphI 826 GCATGC GCATG^C Sse8387I 66 CCTGCAGG CCTGCA^GG Tru9I 856 TTAA T^TAA ################################### # TWO CUT ENZYMES ( 24 enzymes) # ################################### AlwNI 99 CAGCTTCTG CAG[ACGT][ACGT][ACGT]^CTG 637 CAGAGACTG AspHI 367 GAGCAC G[AT]GC[AT]^C 850 GAGCTC BbeI 9 GGCGCC GGCGC^C 387 GGCGCC BbsI 819 GAAGAC GAAGAC(2/6) 1325 GTCTTC BpuAI 819 GAAGAC GAAGAC(2/6) 1325 GTCTTC BsiHKAI 367 GAGCAC G[AT]GC[AT]^C 850 GAGCTC BsrI 1040 ACTGG ACTGG(1/-1) 1212 ACTGG BssHII 146 GCGCGC G^CGCGC 287 GCGCGC EarI 1202 CTCTTC CTCTTC(1/4) 1315 CTCTTC EcoT14I 809 CCATGG C^C[AT][AT]GG 952 CCATGG KasI 9 GGCGCC G^GCGCC 387 GGCGCC Ksp632I 1202 CTCTTC CTCTTC(1/4) 1315 CTCTTC MaeIII 781 GTCAC ^GT[ACGT]AC 1279 GTCAC NarI 9 GGCGCC GG^CGCC 387 GGCGCC NcoI 809 CCATGG C^CATGG 952 CCATGG PvuII 220 CAGCTG CAG^CTG 598 CAGCTG SfoI 9 GGCGCC GGC^GCC 387 GGCGCC SmaI 16 CCCGGG CCC^GGG 271 CCCGGG StyI 809 CCATGG C^C[AT][AT]GG 952 CCATGG Tsp45I 781 GTCAC ^GT[GC]AC 1279 GTCAC Tsp509I 1263 AATT ^AATT 1402 AATT XcmI 212 CCAACCGGCAGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG 1311 CCAACTCTTCTCTGG XmaCI 16 CCCGGG C^CCGGG 271 CCCGGG XmaI 16 CCCGGG C^CCGGG 271 CCCGGG ######################################### # 3 OR MORE CUT ENZYMES ( 75 enzymes) # ######################################### AccII AciI AcyI AluI AlwI ApaI AvaI AvaII BanI BanII BbvI BcnI BmyI BpmI BsaHI BsaJI BseRI BsiYI BslI BsoBI Bsp1286I BsrFI BssKI BstF5I BstNI BstUI BstYI Cac8I CfoI Cfr10I Cfr13I DdeI DpnII DraII DsaI EcoO109I EcoRII FauI Fnu4HI FokI HaeII HaeIII HapII HgaI HhaI Hin1I HinP1I HinfI HpaII ItaI MboI MboII MflI MnlI MspA1I MspI MvaI MvnI MwoI NaeI NciI NdeII NgoMIV NlaIII NlaIV PleI PstI Sau3AI Sau96I ScrFI SfaNI SfcI TseI TspRI XhoII