(Circular) (Six-base) MAPSORT of: pFN21AE1151.seq Check: 6965 from: 1 to: 6163 pFN21AE1151 6163 bp With 182 enzymes: SgfI * July 6, 2012 17:34 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3973 278 Size: 53 83 186 936 2437 2468 Fragments arranged by size: 2468 2437 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2166 3506 1937 Size: 229 1340 4594 Fragments arranged by size: 4594 1340 229 Acc65I G'GTAC_C Cuts at: 3484 3484 Size: 6163 AclI AA'CG_TT Cuts at: 1563 4290 4663 1563 Size: 2727 373 3063 Fragments arranged by size: 3063 2727 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2533 2711 2843 4226 5610 5878 1632 Size: 901 178 132 1383 1384 268 1917 Fragments arranged by size: 1917 1384 1383 901 268 178 132 AfeI AGC'GCT Cuts at: 2546 2546 Size: 6163 AflII C'TTAA_G Cuts at: 820 1017 2860 820 Size: 197 1843 4123 Fragments arranged by size: 4123 1843 197 AflIII A'CryG_T Cuts at: 1203 5062 6039 1203 Size: 3859 977 1327 Fragments arranged by size: 3859 1327 977 AgeI A'CCGG_T Cuts at: 4994 4994 Size: 6163 AhdI GACnn_n'nnGTC Cuts at: 4892 4892 Size: 6163 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 6163 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 6163 AlwNI CAG_nnn'CTG Cuts at: 2109 2647 3906 5478 2109 Size: 538 1259 1572 2794 Fragments arranged by size: 2794 1572 1259 538 ApaI G_GGCC'C Cuts at: 2192 2277 3154 2192 Size: 85 877 5201 Fragments arranged by size: 5201 877 85 ApaLI G'TGCA_C Cuts at: 4220 5376 4220 Size: 1156 5007 ApoI r'AATT_y Cuts at: 1493 3267 3472 3698 3850 5959 1493 Size: 1774 205 226 152 2109 1697 Fragments arranged by size: 2109 1774 1697 226 205 152 AseI AT'TA_AT Cuts at: 160 1032 4717 160 Size: 872 3685 1606 Fragments arranged by size: 3685 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6163 AvaI C'yCGr_G Cuts at: 1870 1943 2021 2203 2276 2369 2908 3488 1870 Size: 73 78 182 73 93 539 580 4545 Fragments arranged by size: 4545 580 539 182 93 78 73 73 BamHI G'GATC_C Cuts at: 2890 3493 3860 2890 Size: 603 367 5193 Fragments arranged by size: 5193 603 367 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2014 2049 2392 2721 3484 Size: 325 217 627 227 35 343 329 763 Cuts at: 3484 4939 618 Size: 1455 1842 Fragments arranged by size: 1842 1455 763 627 343 329 325 227 217 35 BanII G_rGCy'C Cuts at: 1391 2192 2277 2859 3154 3482 1391 Size: 801 85 582 295 328 4072 Fragments arranged by size: 4072 801 582 328 295 85 BbeI G_GCGC'C Cuts at: 2018 2396 2018 Size: 378 5785 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2831 3323 928 Size: 971 932 492 3768 Fragments arranged by size: 3768 971 932 492 BbvCI CC'TCA_GC Cuts at: 2606 2606 Size: 6163 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4354 4388 1903 Size: 34 2417 34 3678 Fragments arranged by size: 3678 2417 34 34 BciVI GTATCCnnnnn_n' Cuts at: 4055 5265 4055 Size: 1210 4953 BclI T'GATC_A Cuts at: 1548 3454 1548 Size: 1906 4257 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4774 136 Size: 107 122 71 631 605 3102 1525 Fragments arranged by size: 3102 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 6158 6158 Size: 6163 BlpI GC'TnA_GC Cuts at: 3580 3580 Size: 6163 Bme1580I G_kGCm'C Cuts at: 1790 2054 2192 2277 3154 4224 5380 1790 Size: 264 138 85 877 1070 1156 2573 Fragments arranged by size: 2573 1156 1070 877 264 138 85 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 6163 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3055 3894 4852 Size: 416 53 83 399 24 1638 839 958 Cuts at: 4852 442 Size: 1753 Fragments arranged by size: 1753 1638 958 839 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6163 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2417 2636 2768 4823 1239 Size: 1178 219 132 2055 2579 Fragments arranged by size: 2579 2055 1178 219 132 Bpu10I CC'TnA_GC Cuts at: 2312 2606 2768 2982 2312 Size: 294 162 214 5493 Fragments arranged by size: 5493 294 214 162 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 4288 5153 5451 4288 Size: 865 298 5000 Fragments arranged by size: 5000 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4826 882 Size: 617 3327 2219 Fragments arranged by size: 3327 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 3954 493 Size: 711 2750 2702 Fragments arranged by size: 2750 2702 711 BsaBI GATnn'nnATC Cuts at: 3456 6157 3456 Size: 2701 3462 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2015 2163 2361 2393 Size: 53 83 186 936 482 148 198 32 Cuts at: 2393 3970 4352 4987 275 Size: 1577 382 635 1451 Fragments arranged by size: 1577 1451 936 635 482 382 198 186 148 83 53 32 BsaWI w'CCGG_w Cuts at: 4596 4994 5268 5415 5826 4596 Size: 398 274 147 411 4933 Fragments arranged by size: 4933 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 5938 Fragments arranged by size: 5938 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2342 2606 2639 2775 2863 3357 1186 Size: 1156 264 33 136 88 494 3992 Fragments arranged by size: 3992 1156 494 264 136 88 33 BseYI C'CCAG_C Cuts at: 2257 2349 5366 5862 2257 Size: 92 3017 496 2558 Fragments arranged by size: 3017 2558 496 92 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2963 1163 Size: 573 1227 4363 Fragments arranged by size: 4363 1227 573 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3613 4374 5402 1540 Size: 275 186 1612 761 1028 2301 Fragments arranged by size: 2301 1612 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2376 2859 4224 4309 5380 2376 Size: 483 1365 85 1071 3159 Fragments arranged by size: 3159 1365 1071 483 85 BsmI GAATG_Cn' Cuts at: 3674 3767 3674 Size: 93 6070 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2054 2192 2277 2376 2859 3154 3482 Size: 399 264 138 85 99 483 295 328 Cuts at: 3482 4224 4309 5380 1391 Size: 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 483 399 328 295 264 138 99 85 85 BspEI T'CCGG_A Cuts at: 5826 5826 Size: 6163 BspHI T'CATG_A Cuts at: 4052 5782 4052 Size: 1730 4433 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2940 3518 844 Size: 2096 578 3489 Fragments arranged by size: 3489 2096 578 BsrBI CCG'CTC Cuts at: 1394 1823 2029 4050 1394 Size: 429 206 2021 3507 Fragments arranged by size: 3507 2021 429 206 BsrDI GCAATG_nn' Cuts at: 65 1248 4658 4832 65 Size: 1183 3410 174 1396 Fragments arranged by size: 3410 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2080 2220 2363 2411 2664 3260 4807 Size: 110 270 140 143 48 253 596 1547 Cuts at: 4807 4994 1700 Size: 187 2869 Fragments arranged by size: 2869 1547 596 270 253 187 143 140 110 48 BsrGI T'GTAC_A Cuts at: 96 96 Size: 6163 BssHII G'CGCG_C Cuts at: 1925 2151 2292 1925 Size: 226 141 5796 Fragments arranged by size: 5796 226 141 BssSI C'ACGA_G Cuts at: 4223 5235 4223 Size: 1012 5151 BstAPI GCAn_nnn'nTGC Cuts at: 794 2647 794 Size: 1853 4310 BstBI TT'CG_AA Cuts at: 4967 4967 Size: 6163 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 6163 BstYI r'GATC_y Cuts at: 1972 2890 3293 3430 3493 3860 4245 4262 5714 Size: 918 403 137 63 367 385 17 1452 Cuts at: 5714 5800 5812 6158 1972 Size: 86 12 346 1977 Fragments arranged by size: 1977 1452 918 403 385 367 346 137 86 63 17 12 Bsu36I CC'TnA_GG Cuts at: 2114 2114 Size: 6163 BtgI C'CryG_G Cuts at: 513 1065 2004 2814 2898 2957 513 Size: 552 939 810 84 59 3719 Fragments arranged by size: 3719 939 810 552 84 59 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 2302 487 Size: 683 56 1076 4348 Fragments arranged by size: 4348 1076 683 56 BtsI GCAGTG_nn' Cuts at: 2186 2944 3101 3679 4473 4493 2186 Size: 758 157 578 794 20 3856 Fragments arranged by size: 3856 794 758 578 157 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6163 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6128 DraI TTT'AAA Cuts at: 3467 3467 Size: 6163 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5170 809 Size: 507 3854 1802 Fragments arranged by size: 3854 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2098 3610 4499 Size: 54 1425 50 275 18 268 1512 889 Cuts at: 4499 6128 8 Size: 1629 43 Fragments arranged by size: 1629 1512 1425 889 275 268 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3610 1537 Size: 275 1798 4090 Fragments arranged by size: 4090 1798 275 EarI CTCTTCn'nnn_ Cuts at: 1353 3213 3326 4093 1353 Size: 1860 113 767 3423 Fragments arranged by size: 3423 1860 767 113 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2636 2717 4750 5124 5270 387 Size: 1064 138 1047 81 2033 374 146 1280 Fragments arranged by size: 2033 1280 1064 1047 374 146 138 81 EcoICRI GAG'CTC Cuts at: 2857 2857 Size: 6163 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2417 2533 2636 2711 2768 2843 4226 Size: 393 785 116 103 75 57 75 1383 Cuts at: 4226 4823 5610 5878 1239 Size: 597 787 268 1524 Fragments arranged by size: 1524 1383 787 785 597 393 268 116 103 75 75 57 EcoO109I rG'GnC_Cy Cuts at: 2188 2189 2273 2308 2686 3151 2188 Size: 1 84 35 378 465 5200 Fragments arranged by size: 5200 465 378 84 35 1 EcoRI G'AATT_C Cuts at: 3472 3850 3472 Size: 378 5785 FspI TGC'GCA Cuts at: 2936 4669 2936 Size: 1733 4430 HaeII r_GCGC'y Cuts at: 1678 2018 2345 2396 2548 2731 5310 1678 Size: 340 327 51 152 183 2579 2531 Fragments arranged by size: 2579 2531 340 327 183 152 51 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 3285 3317 4804 4836 4878 Size: 32 135 32 1713 32 1487 32 42 Cuts at: 4878 4910 1373 Size: 32 2626 Fragments arranged by size: 2626 1713 1487 135 42 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3507 3753 669 Size: 988 281 1569 246 3079 Fragments arranged by size: 3079 1569 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 6163 HpaI GTT'AAC Cuts at: 3753 3753 Size: 6163 KasI G'GCGC_C Cuts at: 2014 2392 2014 Size: 378 5785 KpnI G_GTAC'C Cuts at: 3488 3488 Size: 6163 MfeI C'AATT_G Cuts at: 3762 3762 Size: 6163 MluI A'CGCG_T Cuts at: 6039 6039 Size: 6163 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2124 2445 3238 5277 5461 1305 Size: 819 321 793 2039 184 2007 Fragments arranged by size: 2039 2007 819 793 321 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 4341 Fragments arranged by size: 4341 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2813 4121 4480 4639 518 Size: 648 1647 1308 359 159 2042 Fragments arranged by size: 2042 1647 1308 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2227 2398 2605 3579 4256 5404 5649 1606 Size: 621 171 207 974 677 1148 245 2120 Fragments arranged by size: 2120 1148 974 677 621 245 207 171 NaeI GCC'GGC Cuts at: 2365 2413 2666 2365 Size: 48 253 5862 Fragments arranged by size: 5862 253 48 NarI GG'CG_CC Cuts at: 2015 2393 2015 Size: 378 5785 NcoI C'CATG_G Cuts at: 513 1065 2004 2814 2957 513 Size: 552 939 810 143 3719 Fragments arranged by size: 3719 939 810 552 143 NdeI CA'TA_TG Cuts at: 387 387 Size: 6163 NgoMIV G'CCGG_C Cuts at: 2363 2411 2664 2363 Size: 48 253 5862 Fragments arranged by size: 5862 253 48 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6163 NotI GC'GGCC_GC Cuts at: 3610 3610 Size: 6163 NspI r_CATG'y Cuts at: 1132 1229 2835 3521 5066 1132 Size: 97 1606 686 1545 2229 Fragments arranged by size: 2229 1606 1545 686 97 PasI CC'CwG_GG Cuts at: 1348 2737 2843 1348 Size: 1389 106 4668 Fragments arranged by size: 4668 1389 106 PciI A'CATG_T Cuts at: 5062 5062 Size: 6163 PflMI CCAn_nnn'nTGG Cuts at: 1108 2010 1108 Size: 902 5261 PmeI GTTT'AAAC Cuts at: 3467 3467 Size: 6163 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4232 4264 5745 5777 4232 Size: 32 1481 32 4618 Fragments arranged by size: 4618 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2308 2308 Size: 6163 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6163 PsiI TTA'TAA Cuts at: 3733 3733 Size: 6163 PspOMI G'GGCC_C Cuts at: 2188 2273 3150 2188 Size: 85 877 5201 Fragments arranged by size: 5201 877 85 PstI C_TGCA'G Cuts at: 830 2076 2403 2643 2715 3515 830 Size: 1246 327 240 72 800 3478 Fragments arranged by size: 3478 1246 800 327 240 72 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6163 PvuII CAG'CTG Cuts at: 2227 2605 2227 Size: 378 5785 SacI G_AGCT'C Cuts at: 2859 2859 Size: 6163 SalI G'TCGA_C Cuts at: 1936 3505 1936 Size: 1569 4594 SbfI CC_TGCA'GG Cuts at: 2076 3515 2076 Size: 1439 4724 ScaI AGT'ACT Cuts at: 4411 6123 4411 Size: 1712 4451 SexAI A'CCwGG_T Cuts at: 2421 2421 Size: 6163 SfcI C'TryA_G Cuts at: 826 1045 2072 2399 2639 2711 3511 4646 5327 Size: 219 1027 327 240 72 800 1135 681 Cuts at: 5327 5518 826 Size: 191 1471 Fragments arranged by size: 1471 1135 1027 800 681 327 240 219 191 72 SfoI GGC'GCC Cuts at: 2016 2394 2016 Size: 378 5785 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6163 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6163 SmaI CCC'GGG Cuts at: 1872 2023 2278 3490 1872 Size: 151 255 1212 4545 Fragments arranged by size: 4545 1212 255 151 SmlI C'TyrA_G Cuts at: 820 1017 1943 2860 4267 5168 5430 820 Size: 197 926 917 1407 901 262 1553 Fragments arranged by size: 1553 1407 926 917 901 262 197 SnaBI TAC'GTA Cuts at: 493 3954 493 Size: 3461 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6163 SphI G_CATG'C Cuts at: 1132 2835 3521 1132 Size: 1703 686 3774 Fragments arranged by size: 3774 1703 686 SspI AAT'ATT Cuts at: 5 52 4087 5 Size: 47 4035 2081 Fragments arranged by size: 4035 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 6163 StyI C'CwwG_G Cuts at: 513 1065 2004 2814 2957 3602 513 Size: 552 939 810 143 645 3074 Fragments arranged by size: 3074 939 810 645 552 143 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2341 3397 4190 4207 4360 4545 4990 Size: 1255 761 1056 793 17 153 185 445 Cuts at: 4990 325 Size: 1498 Fragments arranged by size: 1498 1255 1056 793 761 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 3177 4409 6121 Size: 274 80 33 51 1443 1200 1232 1712 Cuts at: 6121 96 Size: 138 Fragments arranged by size: 1712 1443 1232 1200 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2033 2065 6123 6155 2033 Size: 32 4058 32 2041 Fragments arranged by size: 4058 2041 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 6163 XbaI T'CTAG_A Cuts at: 3499 3499 Size: 6163 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2224 3323 2224 Size: 1099 5064 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6163 XmaI C'CCGG_G Cuts at: 1870 2021 2276 3488 1870 Size: 151 255 1212 4545 Fragments arranged by size: 4545 1212 255 151 XmnI GAAnn'nnTTC Cuts at: 4292 4292 Size: 6163 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3971 276 Size: 53 83 186 936 2437 2468 Fragments arranged by size: 2468 2437 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AfeI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PmeI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI PvuII SacI SalI SbfI ScaI SexAI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AloI AscI AvrII BaeI BfrBI BplI BsiWI BsmBI BstXI BstZ17I DraIII EcoNI EcoRV FalI FseI FspAI NruI NsiI PacI PfoI PmlI PspXI PsrI RsrII SacII SanDI SapI SfiI SrfI SwaI