(Linear) (Six-base) MAPSORT of: KIEE1151.seq Check: 1095 from: 1 to: 1458 KIEE1151.orf With 182 enzymes: SgfI * July 3, 2012 16:47 .. AccI GT'mk_AC Cuts at: 0 161 1458 Size: 161 1297 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 0 528 706 838 1458 Size: 528 178 132 620 Fragments arranged by size: 620 528 178 132 AfeI AGC'GCT Cuts at: 0 541 1458 Size: 541 917 AflII C'TTAA_G Cuts at: 0 855 1458 Size: 855 603 AlwNI CAG_nnn'CTG Cuts at: 0 104 642 1458 Size: 104 538 816 Fragments arranged by size: 816 538 104 ApaI G_GGCC'C Cuts at: 0 187 272 1149 1458 Size: 187 85 877 309 Fragments arranged by size: 877 309 187 85 ApoI r'AATT_y Cuts at: 0 1262 1458 Size: 1262 196 AvaI C'yCGr_G Cuts at: 0 16 198 271 364 903 1458 Size: 16 182 73 93 539 555 Fragments arranged by size: 555 539 182 93 73 16 BamHI G'GATC_C Cuts at: 0 885 1458 Size: 885 573 BanI G'GyrC_C Cuts at: 0 9 44 387 716 1458 Size: 9 35 343 329 742 Fragments arranged by size: 742 343 329 35 9 BanII G_rGCy'C Cuts at: 0 187 272 854 1149 1458 Size: 187 85 582 295 309 Fragments arranged by size: 582 309 295 187 85 BbeI G_GCGC'C Cuts at: 0 13 391 1458 Size: 13 378 1067 Fragments arranged by size: 1067 378 13 BbsI GAAGACnn'nnnn_ Cuts at: 0 826 1318 1458 Size: 826 492 140 Fragments arranged by size: 826 492 140 BbvCI CC'TCA_GC Cuts at: 0 601 1458 Size: 601 857 BclI T'GATC_A Cuts at: 0 1449 1458 Size: 1449 9 Bme1580I G_kGCm'C Cuts at: 0 49 187 272 1149 1458 Size: 49 138 85 877 309 Fragments arranged by size: 877 309 138 85 49 BmrI ACTGGGnnnn_n' Cuts at: 0 1050 1458 Size: 1050 408 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 412 631 763 1458 Size: 412 219 132 695 Fragments arranged by size: 695 412 219 132 Bpu10I CC'TnA_GC Cuts at: 0 307 601 763 977 1458 Size: 307 294 162 214 481 Fragments arranged by size: 481 307 294 214 162 BsaBI GATnn'nnATC Cuts at: 0 1451 1458 Size: 1451 7 BsaHI Gr'CG_yC Cuts at: 0 10 158 356 388 1458 Size: 10 148 198 32 1070 Fragments arranged by size: 1070 198 148 32 10 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 0 337 601 634 770 858 1352 1458 Size: 337 264 33 136 88 494 106 Fragments arranged by size: 494 337 264 136 106 88 33 BseYI C'CCAG_C Cuts at: 0 252 344 1458 Size: 252 92 1114 Fragments arranged by size: 1114 252 92 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 0 958 1458 Size: 958 500 BsiHKAI G_wGCw'C Cuts at: 0 371 854 1458 Size: 371 483 604 Fragments arranged by size: 604 483 371 Bsp1286I G_dGCh'C Cuts at: 0 49 187 272 371 854 1149 1458 Size: 49 138 85 99 483 295 309 Fragments arranged by size: 483 309 295 138 99 85 49 BspMI ACCTGCnnnn'nnnn_ Cuts at: 0 935 1458 Size: 935 523 BsrBI CCG'CTC Cuts at: 0 24 1458 Size: 24 1434 BsrFI r'CCGG_y Cuts at: 0 75 215 358 406 659 1255 1458 Size: 75 140 143 48 253 596 203 Fragments arranged by size: 596 253 203 143 140 75 48 BssHII G'CGCG_C Cuts at: 0 146 287 1458 Size: 146 141 1171 Fragments arranged by size: 1171 146 141 BstAPI GCAn_nnn'nTGC Cuts at: 0 642 1458 Size: 642 816 BstYI r'GATC_y Cuts at: 0 885 1288 1425 1458 Size: 885 403 137 33 Fragments arranged by size: 885 403 137 33 Bsu36I CC'TnA_GG Cuts at: 0 109 1458 Size: 109 1349 BtgI C'CryG_G Cuts at: 0 809 893 952 1458 Size: 809 84 59 506 Fragments arranged by size: 809 506 84 59 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 0 297 1458 Size: 297 1161 BtsI GCAGTG_nn' Cuts at: 0 181 939 1096 1458 Size: 181 758 157 362 Fragments arranged by size: 758 362 181 157 EaeI y'GGCC_r Cuts at: 0 93 1458 Size: 93 1365 EarI CTCTTCn'nnn_ Cuts at: 0 1208 1321 1458 Size: 1208 113 137 Fragments arranged by size: 1208 137 113 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 0 631 712 1458 Size: 631 81 746 Fragments arranged by size: 746 631 81 EcoICRI GAG'CTC Cuts at: 0 852 1458 Size: 852 606 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 0 412 528 631 706 763 838 1458 Size: 412 116 103 75 57 75 620 Fragments arranged by size: 620 412 116 103 75 75 57 EcoO109I rG'GnC_Cy Cuts at: 0 183 184 268 303 681 1146 1458 Size: 183 1 84 35 378 465 312 Fragments arranged by size: 465 378 312 183 84 35 1 FspI TGC'GCA Cuts at: 0 931 1458 Size: 931 527 HaeII r_GCGC'y Cuts at: 0 13 340 391 543 726 1458 Size: 13 327 51 152 183 732 Fragments arranged by size: 732 327 183 152 51 13 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 0 1280 1312 1458 Size: 1280 32 146 Fragments arranged by size: 1280 146 32 KasI G'GCGC_C Cuts at: 0 9 387 1458 Size: 9 378 1071 Fragments arranged by size: 1071 378 9 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 0 119 440 1233 1458 Size: 119 321 793 225 Fragments arranged by size: 793 321 225 119 MslI CAynn'nnrTG Cuts at: 0 808 1458 Size: 808 650 MspA1I CmG'CkG Cuts at: 0 222 393 600 1458 Size: 222 171 207 858 Fragments arranged by size: 858 222 207 171 NaeI GCC'GGC Cuts at: 0 360 408 661 1458 Size: 360 48 253 797 Fragments arranged by size: 797 360 253 48 NarI GG'CG_CC Cuts at: 0 10 388 1458 Size: 10 378 1070 Fragments arranged by size: 1070 378 10 NcoI C'CATG_G Cuts at: 0 809 952 1458 Size: 809 143 506 Fragments arranged by size: 809 506 143 NgoMIV G'CCGG_C Cuts at: 0 358 406 659 1458 Size: 358 48 253 799 Fragments arranged by size: 799 358 253 48 NspI r_CATG'y Cuts at: 0 830 1458 Size: 830 628 PasI CC'CwG_GG Cuts at: 0 732 838 1458 Size: 732 106 620 Fragments arranged by size: 732 620 106 PpuMI rG'GwC_Cy Cuts at: 0 303 1458 Size: 303 1155 PspOMI G'GGCC_C Cuts at: 0 183 268 1145 1458 Size: 183 85 877 313 Fragments arranged by size: 877 313 183 85 PstI C_TGCA'G Cuts at: 0 71 398 638 710 1458 Size: 71 327 240 72 748 Fragments arranged by size: 748 327 240 72 71 PvuII CAG'CTG Cuts at: 0 222 600 1458 Size: 222 378 858 Fragments arranged by size: 858 378 222 SacI G_AGCT'C Cuts at: 0 854 1458 Size: 854 604 SbfI CC_TGCA'GG Cuts at: 0 71 1458 Size: 71 1387 SexAI A'CCwGG_T Cuts at: 0 416 1458 Size: 416 1042 SfcI C'TryA_G Cuts at: 0 67 394 634 706 1458 Size: 67 327 240 72 752 Fragments arranged by size: 752 327 240 72 67 SfoI GGC'GCC Cuts at: 0 11 389 1458 Size: 11 378 1069 Fragments arranged by size: 1069 378 11 SmaI CCC'GGG Cuts at: 0 18 273 1458 Size: 18 255 1185 Fragments arranged by size: 1185 255 18 SmlI C'TyrA_G Cuts at: 0 855 1458 Size: 855 603 SphI G_CATG'C Cuts at: 0 830 1458 Size: 830 628 StyI C'CwwG_G Cuts at: 0 809 952 1458 Size: 809 143 506 Fragments arranged by size: 809 506 143 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 0 336 1392 1458 Size: 336 1056 66 Fragments arranged by size: 1056 336 66 TatI w'GTAC_w Cuts at: 0 1172 1458 Size: 1172 286 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 0 28 60 1458 Size: 28 32 1398 Fragments arranged by size: 1398 32 28 XcmI CCAnnnn_n'nnnnTGG Cuts at: 0 219 1318 1458 Size: 219 1099 140 Fragments arranged by size: 1099 219 140 XmaI C'CCGG_G Cuts at: 0 16 271 1458 Size: 16 255 1187 Fragments arranged by size: 1187 255 16 Enzymes that do cut: AccI AcuI AfeI AflII AlwNI ApaI ApoI AvaI BamHI BanI BanII BbeI BbsI BbvCI BclI Bme1580I BmrI BpmI Bpu10I BsaBI BsaHI BseRI BseYI BsgI BsiHKAI Bsp1286I BspMI BsrBI BsrFI BssHII BstAPI BstYI Bsu36I BtgI BtgZI BtsI EaeI EarI EciI EcoICRI Eco57MI EcoO109I FspI HaeII Hin4I KasI MmeI MslI MspA1I NaeI NarI NcoI NgoMIV NspI PasI PpuMI PspOMI PstI PvuII SacI SbfI SexAI SfcI SfoI SmaI SmlI SphI StyI TaqII TatI TstI XcmI XmaI Enzymes that do not cut: AarI AatII Acc65I AclI AflIII AgeI AhdI AleI AlfI AloI ApaLI AscI AseI AsiSI AvrII BaeI BcgI BciVI BfrBI BglI BglII BlpI BmgBI BmtI BplI BpuEI BsaI BsaAI BsaWI BsaXI BsiEI BsiWI BsmI BsmBI BspEI BspHI BsrDI BsrGI BssSI BstBI BstEII BstXI BstZ17I ClaI CspCI DraI DraIII DrdI EagI EcoNI EcoRI EcoRV FalI FseI FspAI HincII HindIII HpaI KpnI MfeI MluI MscI NdeI NheI NotI NruI NsiI PacI PciI PflMI PfoI PmeI PmlI PpiI PshAI PsiI PspXI PsrI PvuI RsrII SacII SalI SanDI SapI ScaI SfiI SgfI SgrAI SnaBI SpeI SrfI SspI StuI SwaI Tth111I XbaI XhoI XmnI ZraI