Restriction map for
pFN21AB9928
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# NO CUT ENZYMES ( 119 enzymes) #
##################################
AatII Acc65I AccI AccII AclI
AcyI AflII AgeI AhdI Alw44I
AlwNI AocI ApaI ApaLI AscI
AseI AsnI Asp700I Asp718I AspEI
AviII AvrII BamHI BbeI BclI
BfrI BglII BlnI BlpI Bpu1102I
BsaBI BsaHI BsiWI BsmBI Bsp1407I
BspDI BspHI BspLU11I BspMI BsrBI
BsrFI BsrGI BssHII BssSI Bst1107I
BstBI BstEII BstPI BstUI BstZ17I
Bsu36I CelII Cfr10I ClaI CpoI
DraI DraIII DrdI Eam1105I Eco81I
EcoO65I EcoRV EcoT22I FbaI FseI
FspI HgaI Hin1I HindIII HpaI
KasI KpnI KspI MamI MfeI
MluI MunI MvnI NaeI NarI
NdeI NgoMIV NotI NruI NsiI
NspV PacI PaeR7I PflMI PinAI
PmeI PshAI PshBI Psp1406I PvuI
RcaI RsrII SacI SacII SalI
SbfI SexAI SfiI SfoI SfuI
SgrAI SmaI SnaBI SpeI Sse8387I
SspBI SspI SwaI Van91I XbaI
XhoI XmaCI XmaI XmnI
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# ONE CUT ENZYMES ( 57 enzymes) #
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AccIII 321 TCCGGA T^CCGGA
AflIII 1460 ACGTGT A^C[GA][CT]GT
Aor51HI 514 AGCGCT AGC^GCT
AspHI 3 GTGCTC G[AT]GC[AT]^C
AspI 1223 GACAGGGTC GAC[ACGT]^[ACGT][ACGT]GTC
AvaI 1299 CCCGAG C^[CT]CG[GA]G
BalI 1812 TGGCCA TGG^CCA
BanI 1466 GGTGCC G^G[CT][GA]CC
BanII 1693 GAGCCC G[GA]GC[CT]^C
BbrPI 300 CACGTG CAC^GTG
BbsI 280 GAAGAC GAAGAC(2/6)
BcgI 760 CGAAGAAACTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BciVI 355 GGATAC GTATCC(6/5)
BglI 1064 GCCTGGACGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BpuAI 280 GAAGAC GAAGAC(2/6)
BsaAI 300 CACGTG [CT]AC^GT[GA]
BseAI 321 TCCGGA T^CCGGA
BsiEI 1071 CGGCCG CG[GA][CT]^CG
BsiHKAI 3 GTGCTC G[AT]GC[AT]^C
BsmFI 25 GGGAC GGGAC(10/14)
BsmI 49 GAATGC GAATGC(1/-1)
BsoBI 1299 CCCGAG C^[CT]CG[GA]G
BspEI 321 TCCGGA T^CCGGA
DraII 1352 AGGACCC [GA]G^G[ACGT]CC[CT]
DsaI 60 CCATGG C^C[GA][CT]GG
EagI 1071 CGGCCG C^GGCCG
EarI 967 GAAGAG CTCTTC(1/4)
EclXI 1071 CGGCCG C^GGCCG
Eco47III 514 AGCGCT AGC^GCT
Eco52I 1071 CGGCCG C^GGCCG
EcoNI 1701 CCTCTCCCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO109I 1352 AGGACCC [GA]G^G[ACGT]CC[CT]
EcoRI 423 GAATTC G^AATTC
HincII 1099 GTTGAC GT[CT]^[GA]AC
HindII 1099 GTTGAC GT[CT]^[GA]AC
Ksp632I 967 GAAGAG CTCTTC(1/4)
MluNI 1812 TGGCCA TGG^CCA
MroI 321 TCCGGA T^CCGGA
MscI 1812 TGGCCA TGG^CCA
MseI 374 TTAA T^TAA
MspA1I 850 CAGCTG C[AC]G^C[GT]G
NcoI 60 CCATGG C^CATGG
NheI 511 GCTAGC G^CTAGC
NspI 490 GCATGC [GA]CATG^[CT]
PflFI 1223 GACAGGGTC GAC[ACGT]^[ACGT][ACGT]GTC
PmaCI 300 CACGTG CAC^GTG
PmlI 300 CACGTG CAC^GTG
PpuMI 1352 AGGACCC [GA]G^G[AT]CC[CT]
PstI 1186 CTGCAG CTGCA^G
PvuII 850 CAGCTG CAG^CTG
SapI 967 GAAGAGC GCTCTTC(1/4)
ScaI 1316 AGTACT AGT^ACT
SfcI 1186 CTGCAG C^T[GA][CT]AG
SmlI 1348 CTCAAG C^T[CT][GA]AG
SphI 490 GCATGC GCATG^C
Tru9I 374 TTAA T^TAA
Tth111I 1223 GACAGGGTC GAC[ACGT]^[ACGT][ACGT]GTC
###################################
# TWO CUT ENZYMES ( 17 enzymes) #
###################################
AcsI 423 GAATTC [GA]^AATT[CT]
801 AAATTT
AlwI 1848 GATCC GGATC(4/5)
1150 GGATC
ApoI 423 GAATTC [GA]^AATT[CT]
801 AAATTT
BbvCI 1440 CCTCAGC CCTCAGC(-5/-2)
1377 GCTGAGG
BmyI 3 GTGCTC G[AGT]GC[ACT]^C
1693 GAGCCC
BsaWI 321 TCCGGA [AT]^CCGG[AT]
459 TCCGGT
BsgI 996 GTGCAG GTGCAG(16/14)
1726 GTGCAG
Bsp1286I 3 GTGCTC G[AGT]GC[ACT]^C
1693 GAGCCC
BsrDI 439 CATTGC GCAATG(2/0)
1566 CATTGC
BstYI 1150 GGATCT [GA]^GATC[CT]
1847 AGATCC
EaeI 1071 CGGCCG [CT]^GGCC[GA]
1812 TGGCCA
FauI 159 CCCGC CCCGC(4/6)
1852 CCCGC
HaeII 155 AGCGCC [GA]GCGC^[CT]
514 AGCGCT
HphI 948 GGTGA GGTGA(8/7)
1553 TCACC
MflI 1150 GGATCT [GA]^GATC[CT]
1847 AGATCC
XcmI 174 CCAGGAGAATGATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
911 CCAAAAATAAGCTGG
XhoII 1150 GGATCT [GA]^GATC[CT]
1847 AGATCC
#########################################
# 3 OR MORE CUT ENZYMES ( 65 enzymes) #
#########################################
AciI AfaI AluI AvaII BbvI
BcnI BfaI BpmI BsaI BsaJI
BseRI BsiYI BslI BsmAI BsrI
BssKI BstAPI BstF5I BstNI BstXI
Cac8I CfoI Cfr13I DdeI DpnII
EcoRII EcoT14I Fnu4HI FokI HaeIII
HapII HhaI HinP1I HinfI HpaII
ItaI MaeI MaeII MaeIII MboI
MboII MnlI MslI MspI MvaI
MwoI NciI NdeII NlaIII NlaIV
PleI RsaI Sau3AI Sau96I ScrFI
SfaNI StuI StyI TaqI TfiI
TseI Tsp45I Tsp509I TspRI TthHB8I