(Circular) (Six-base) MAPSORT of: pFN21AB9928.seq Check: 1530 from: 1 to: 6583 pFN21AB9928 6583 bp With 182 enzymes: SgfI * April 15, 2010 09:24 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 4393 278 Size: 53 83 186 936 2857 2468 Fragments arranged by size: 2857 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3926 1937 Size: 1989 4594 Acc65I G'GTAC_C Cuts at: 3904 3904 Size: 6583 AclI AA'CG_TT Cuts at: 1563 4710 5083 1563 Size: 3147 373 3063 Fragments arranged by size: 3147 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2064 2810 2984 3389 3770 4646 6030 6298 Size: 432 746 174 405 381 876 1384 268 Cuts at: 6298 1632 Size: 1917 Fragments arranged by size: 1917 1384 876 746 432 405 381 268 174 AfeI AGC'GCT Cuts at: 2521 2521 Size: 6583 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 6386 AflIII A'CryG_T Cuts at: 1203 3465 5482 6459 1203 Size: 2262 2017 977 1327 Fragments arranged by size: 2262 2017 1327 977 AgeI A'CCGG_T Cuts at: 5414 5414 Size: 6583 AhdI GACnn_n'nnGTC Cuts at: 5312 5312 Size: 6583 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 6583 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 6583 AlwNI CAG_nnn'CTG Cuts at: 4326 5898 4326 Size: 1572 5011 ApaLI G'TGCA_C Cuts at: 4640 5796 4640 Size: 1156 5427 ApoI r'AATT_y Cuts at: 1493 2428 2806 3892 4118 4270 6379 1493 Size: 935 378 1086 226 152 2109 1697 Fragments arranged by size: 2109 1697 1086 935 378 226 152 AseI AT'TA_AT Cuts at: 160 1032 5137 160 Size: 872 4105 1606 Fragments arranged by size: 4105 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6583 AvaI C'yCGr_G Cuts at: 1870 1943 3304 3908 1870 Size: 73 1361 604 4545 Fragments arranged by size: 4545 1361 604 73 BamHI G'GATC_C Cuts at: 3913 4280 3913 Size: 367 6216 BanI G'GyrC_C Cuts at: 618 943 1160 1787 3471 3904 5359 618 Size: 325 217 627 1684 433 1455 1842 Fragments arranged by size: 1842 1684 1455 627 433 325 217 BanII G_rGCy'C Cuts at: 1391 3702 3902 1391 Size: 2311 200 4072 Fragments arranged by size: 4072 2311 200 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2292 928 Size: 971 393 5219 Fragments arranged by size: 5219 971 393 BbvCI CC'TCA_GC Cuts at: 3383 3446 3383 Size: 63 6520 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 2754 2788 4774 4808 1903 Size: 34 817 34 1986 34 3678 Fragments arranged by size: 3678 1986 817 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 2354 4475 5685 2354 Size: 2121 1210 3252 Fragments arranged by size: 3252 2121 1210 BclI T'GATC_A Cuts at: 1548 1548 Size: 6583 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 3075 5194 136 Size: 107 122 71 631 605 1403 2119 1525 Fragments arranged by size: 2119 1525 1403 631 605 122 107 71 BglII A'GATC_T Cuts at: 6578 6578 Size: 6583 BlpI GC'TnA_GC Cuts at: 4000 4000 Size: 6583 Bme1580I G_kGCm'C Cuts at: 1790 4644 5800 1790 Size: 2854 1156 2573 Fragments arranged by size: 2854 2573 1156 BmgBI CAC'GTC Cuts at: 1318 3466 1318 Size: 2148 4435 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3665 3742 4314 Size: 416 53 83 399 24 2248 77 572 Cuts at: 4314 5272 442 Size: 958 1753 Fragments arranged by size: 2248 1753 958 572 416 399 83 77 53 24 BmtI G_CTAG'C Cuts at: 1056 2520 1056 Size: 1464 5119 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2690 2948 3240 3673 3785 3821 5243 1239 Size: 1451 258 292 433 112 36 1422 2579 Fragments arranged by size: 2579 1451 1422 433 292 258 112 36 Bpu10I CC'TnA_GC Cuts at: 2814 3383 3446 2814 Size: 569 63 5951 Fragments arranged by size: 5951 569 63 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3338 4708 5573 5871 3338 Size: 1370 865 298 4050 Fragments arranged by size: 4050 1370 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 3155 3239 3777 5246 882 Size: 617 1656 84 538 1469 2219 Fragments arranged by size: 2219 1656 1469 617 538 84 BsaAI yAC'GTr Cuts at: 493 1204 2307 4374 493 Size: 711 1103 2067 2702 Fragments arranged by size: 2702 2067 1103 711 BsaBI GATnn'nnATC Cuts at: 6577 6577 Size: 6583 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 4390 4772 5407 275 Size: 53 83 186 936 2857 382 635 1451 Fragments arranged by size: 2857 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 2326 2464 5016 5414 5688 5835 6246 2326 Size: 138 2552 398 274 147 411 2663 Fragments arranged by size: 2663 2552 411 398 274 147 138 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2669 2699 2921 2951 3219 Size: 30 165 30 1266 30 222 30 268 Cuts at: 3219 3249 3434 3464 1178 Size: 30 185 30 4297 Fragments arranged by size: 4297 1266 268 222 185 165 30 30 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2143 3227 3676 1186 Size: 957 1084 449 4093 Fragments arranged by size: 4093 1084 957 449 BseYI C'CCAG_C Cuts at: 2688 5786 6282 2688 Size: 3098 496 2989 Fragments arranged by size: 3098 2989 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 3022 3752 1163 Size: 573 1286 730 3994 Fragments arranged by size: 3994 1286 730 573 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3079 4033 4794 5822 1540 Size: 275 186 1078 954 761 1028 2301 Fragments arranged by size: 2301 1078 1028 954 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2012 4644 4729 5800 2012 Size: 2632 85 1071 2795 Fragments arranged by size: 2795 2632 1071 85 BsmI GAATG_Cn' Cuts at: 2060 4094 4187 2060 Size: 2034 93 4456 Fragments arranged by size: 4456 2034 93 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2012 3702 3902 4644 4729 5800 1391 Size: 399 222 1690 200 742 85 1071 2174 Fragments arranged by size: 2174 1690 1071 742 399 222 200 85 BspEI T'CCGG_A Cuts at: 2326 6246 2326 Size: 3920 2663 BspHI T'CATG_A Cuts at: 4472 6202 4472 Size: 1730 4853 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3938 844 Size: 3094 3489 BsrBI CCG'CTC Cuts at: 1394 1823 4470 1394 Size: 429 2647 3507 Fragments arranged by size: 3507 2647 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2443 3570 5078 5252 65 Size: 1183 1195 1127 1508 174 1396 Fragments arranged by size: 1508 1396 1195 1183 1127 174 BsrFI r'CCGG_y Cuts at: 1700 1810 5227 5414 1700 Size: 110 3417 187 2869 Fragments arranged by size: 3417 2869 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 6583 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6583 BssSI C'ACGA_G Cuts at: 4643 5655 4643 Size: 1012 5571 BstAPI GCAn_nnn'nTGC Cuts at: 794 2256 3190 3315 794 Size: 1462 934 125 4062 Fragments arranged by size: 4062 1462 934 125 BstBI TT'CG_AA Cuts at: 5387 5387 Size: 6583 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 6583 BstXI CCAn_nnnn'nTGG Cuts at: 2066 2861 3460 2066 Size: 795 599 5189 Fragments arranged by size: 5189 795 599 BstYI r'GATC_y Cuts at: 1972 3155 3852 3913 4280 4665 4682 6134 6220 Size: 1183 697 61 367 385 17 1452 86 Cuts at: 6220 6232 6578 1972 Size: 12 346 1977 Fragments arranged by size: 1977 1452 1183 697 385 367 346 86 61 17 12 BtgI C'CryG_G Cuts at: 513 1065 2065 513 Size: 552 1000 5031 Fragments arranged by size: 5031 1000 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5844 Fragments arranged by size: 5844 683 56 BtsI GCAGTG_nn' Cuts at: 2173 2455 4099 4893 4913 2173 Size: 282 1644 794 20 3843 Fragments arranged by size: 3843 1644 794 282 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6583 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6548 DraI TTT'AAA Cuts at: 3887 3887 Size: 6583 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5590 809 Size: 507 4274 1802 Fragments arranged by size: 4274 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 3076 3817 4030 Size: 54 1425 50 275 18 1246 741 213 Cuts at: 4030 4919 6548 8 Size: 889 1629 43 Fragments arranged by size: 1629 1425 1246 889 741 275 213 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3076 4030 1537 Size: 275 1264 954 4090 Fragments arranged by size: 4090 1264 954 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2967 4513 1353 Size: 1614 1546 3423 Fragments arranged by size: 3423 1614 1546 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 3013 5170 5544 5690 387 Size: 1064 138 1424 2157 374 146 1280 Fragments arranged by size: 2157 1424 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2064 2690 2810 2948 2984 3240 3389 Size: 393 432 626 120 138 36 256 149 Cuts at: 3389 3673 3770 3785 3821 4646 5243 6030 6298 Size: 284 97 15 36 825 597 787 268 Cuts at: 6298 1239 Size: 1524 Fragments arranged by size: 1524 825 787 626 597 432 393 284 268 256 149 138 120 97 36 36 15 EcoNI CCTnn'n_nnAGG Cuts at: 3710 3710 Size: 6583 EcoO109I rG'GnC_Cy Cuts at: 3358 3358 Size: 6583 EcoRI G'AATT_C Cuts at: 2428 3892 4270 2428 Size: 1464 378 4741 Fragments arranged by size: 4741 1464 378 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 3163 3163 Size: 6583 FspI TGC'GCA Cuts at: 5089 5089 Size: 6583 HaeII r_GCGC'y Cuts at: 1678 2164 2523 5730 1678 Size: 486 359 3207 2531 Fragments arranged by size: 3207 2531 486 359 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2119 2151 2807 2839 2893 Size: 32 135 32 547 32 656 32 54 Cuts at: 2893 2925 3219 3251 3434 3466 5224 5256 5298 Size: 32 294 32 183 32 1758 32 42 Cuts at: 5298 5330 1373 Size: 32 2626 Fragments arranged by size: 2626 1758 656 547 294 183 135 54 42 32 32 32 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3106 3927 4173 669 Size: 988 281 1168 821 246 3079 Fragments arranged by size: 3079 1168 988 821 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 6583 HpaI GTT'AAC Cuts at: 4173 4173 Size: 6583 KpnI G_GTAC'C Cuts at: 3908 3908 Size: 6583 MfeI C'AATT_G Cuts at: 4182 4182 Size: 6583 MluI A'CGCG_T Cuts at: 6459 6459 Size: 6583 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 5697 5881 1305 Size: 4392 184 2007 Fragments arranged by size: 4392 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 3819 10 Size: 54 1425 343 1987 2774 Fragments arranged by size: 2774 1987 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2064 2448 3458 3671 4541 4900 5059 Size: 648 898 384 1010 213 870 359 159 Cuts at: 5059 518 Size: 2042 Fragments arranged by size: 2042 1010 898 870 648 384 359 213 159 MspA1I CmG'CkG Cuts at: 1606 2857 3999 4676 5824 6069 1606 Size: 1251 1142 677 1148 245 2120 Fragments arranged by size: 2120 1251 1148 1142 677 245 NcoI C'CATG_G Cuts at: 513 1065 2065 513 Size: 552 1000 5031 Fragments arranged by size: 5031 1000 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 6583 NheI G'CTAG_C Cuts at: 1052 2516 1052 Size: 1464 5119 NotI GC'GGCC_GC Cuts at: 4030 4030 Size: 6583 NspI r_CATG'y Cuts at: 1132 1229 2499 3941 5486 1132 Size: 97 1270 1442 1545 2229 Fragments arranged by size: 2229 1545 1442 1270 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 6583 PciI A'CATG_T Cuts at: 5482 5482 Size: 6583 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 6583 PfoI T'CCnGG_A Cuts at: 2109 2508 3042 2109 Size: 399 534 5650 Fragments arranged by size: 5650 534 399 PmeI GTTT'AAAC Cuts at: 3887 3887 Size: 6583 PmlI CAC'GTG Cuts at: 2307 2307 Size: 6583 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4652 4684 6165 6197 4652 Size: 32 1481 32 5038 Fragments arranged by size: 5038 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 3358 3358 Size: 6583 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6583 PsiI TTA'TAA Cuts at: 2550 4153 2550 Size: 1603 4980 PsrI GAACnnnnnnTACnnnnnnn_nnnnn' Cuts at: 2660 2692 2660 Size: 32 6551 PstI C_TGCA'G Cuts at: 830 3195 3935 830 Size: 2365 740 3478 Fragments arranged by size: 3478 2365 740 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6583 PvuII CAG'CTG Cuts at: 2857 2857 Size: 6583 SalI G'TCGA_C Cuts at: 1936 3925 1936 Size: 1989 4594 SapI GCTCTTCn'nnn_ Cuts at: 2967 2967 Size: 6583 SbfI CC_TGCA'GG Cuts at: 3935 3935 Size: 6583 ScaI AGT'ACT Cuts at: 3323 4831 6543 3323 Size: 1508 1712 3363 Fragments arranged by size: 3363 1712 1508 SfcI C'TryA_G Cuts at: 826 1045 3191 3931 5066 5747 5938 826 Size: 219 2146 740 1135 681 191 1471 Fragments arranged by size: 2146 1471 1135 740 681 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6583 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6583 SmaI CCC'GGG Cuts at: 1872 3910 1872 Size: 2038 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 3353 4687 5588 5850 820 Size: 197 926 1410 1334 901 262 1553 Fragments arranged by size: 1553 1410 1334 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 4374 493 Size: 3881 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6583 SphI G_CATG'C Cuts at: 1132 2499 3941 1132 Size: 1367 1442 3774 Fragments arranged by size: 3774 1442 1367 SspI AAT'ATT Cuts at: 5 52 4507 5 Size: 47 4455 2081 Fragments arranged by size: 4455 2081 47 StuI AGG'CCT Cuts at: 1517 3260 3824 3866 1517 Size: 1743 564 42 4234 Fragments arranged by size: 4234 1743 564 42 StyI C'CwwG_G Cuts at: 513 1065 2065 3270 3291 4022 513 Size: 552 1000 1205 21 731 3074 Fragments arranged by size: 3074 1205 1000 731 552 21 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3857 4610 4627 4780 4965 5410 325 Size: 1255 2277 753 17 153 185 445 1498 Fragments arranged by size: 2277 1498 1255 753 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 3321 4829 6541 Size: 274 80 33 51 1443 1344 1508 1712 Cuts at: 6541 96 Size: 138 Fragments arranged by size: 1712 1508 1443 1344 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2921 2953 3455 3487 3782 3814 6543 6575 2921 Size: 32 502 32 295 32 2729 32 2929 Fragments arranged by size: 2929 2729 502 295 32 32 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 3231 1316 Size: 1915 4668 XbaI T'CTAG_A Cuts at: 3919 3919 Size: 6583 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2186 2923 2186 Size: 737 5846 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6583 XmaI C'CCGG_G Cuts at: 1870 3908 1870 Size: 2038 4545 XmnI GAAnn'nnTTC Cuts at: 4712 4712 Size: 6583 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 4391 276 Size: 53 83 186 936 2857 2468 Fragments arranged by size: 2857 2468 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AfeI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PfoI PmeI PmlI PpiI PpuMI PshAI PsiI PsrI PstI PvuI PvuII SalI SapI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AloI ApaI AscI AvrII BaeI BbeI BfrBI BplI BsiWI BsmBI BstZ17I Bsu36I DraIII EcoICRI EcoRV FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PspOMI PspXI RsrII SacI SacII SanDI SexAI SfiI SfoI SrfI SwaI