(Circular) (Six-base) MAPSORT of: pFN21AB9979.seq Check: 3958 from: 1 to: 6379 pFN21AB9979 6379 bp With 182 enzymes: SgfI * April 15, 2010 09:24 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 4189 278 Size: 53 83 186 936 2653 2468 Fragments arranged by size: 2653 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3722 1937 Size: 1785 4594 Acc65I G'GTAC_C Cuts at: 3700 3700 Size: 6379 AclI AA'CG_TT Cuts at: 1563 4506 4879 1563 Size: 2943 373 3063 Fragments arranged by size: 3063 2943 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2711 3128 4442 5826 6094 1632 Size: 1079 417 1314 1384 268 1917 Fragments arranged by size: 1917 1384 1314 1079 417 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 6182 AflIII A'CryG_T Cuts at: 1203 5278 6255 1203 Size: 4075 977 1327 Fragments arranged by size: 4075 1327 977 AgeI A'CCGG_T Cuts at: 5210 5210 Size: 6379 AhdI GACnn_n'nnGTC Cuts at: 5108 5108 Size: 6379 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 6379 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 3017 1184 Size: 1833 4546 AlwNI CAG_nnn'CTG Cuts at: 3106 3403 4122 5694 3106 Size: 297 719 1572 3791 Fragments arranged by size: 3791 1572 719 297 ApaI G_GGCC'C Cuts at: 2929 2929 Size: 6379 ApaLI G'TGCA_C Cuts at: 2904 4436 5592 2904 Size: 1532 1156 3691 Fragments arranged by size: 3691 1532 1156 ApoI r'AATT_y Cuts at: 1493 2268 2314 3688 3914 4066 6175 1493 Size: 775 46 1374 226 152 2109 1697 Fragments arranged by size: 2109 1697 1374 775 226 152 46 AseI AT'TA_AT Cuts at: 160 1032 4933 160 Size: 872 3901 1606 Fragments arranged by size: 3901 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6379 AvaI C'yCGr_G Cuts at: 1870 1943 2534 3084 3645 3704 1870 Size: 73 591 550 561 59 4545 Fragments arranged by size: 4545 591 561 550 73 59 BamHI G'GATC_C Cuts at: 3709 4076 3709 Size: 367 6012 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2739 3468 3700 5155 618 Size: 325 217 627 952 729 232 1455 1842 Fragments arranged by size: 1842 1455 952 729 627 325 232 217 BanII G_rGCy'C Cuts at: 1391 2929 3448 3499 3654 3698 1391 Size: 1538 519 51 155 44 4072 Fragments arranged by size: 4072 1538 519 155 51 44 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2102 3150 928 Size: 971 203 1048 4157 Fragments arranged by size: 4157 1048 971 203 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4570 4604 1903 Size: 34 2633 34 3678 Fragments arranged by size: 3678 2633 34 34 BciVI GTATCCnnnnn_n' Cuts at: 2337 4271 5481 2337 Size: 1934 1210 3235 Fragments arranged by size: 3235 1934 1210 BclI T'GATC_A Cuts at: 1548 3462 1548 Size: 1914 4465 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4990 136 Size: 107 122 71 631 605 3318 1525 Fragments arranged by size: 3318 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 2023 6374 2023 Size: 4351 2028 BlpI GC'TnA_GC Cuts at: 3796 3796 Size: 6379 Bme1580I G_kGCm'C Cuts at: 1790 2908 2929 3428 4440 5596 1790 Size: 1118 21 499 1012 1156 2573 Fragments arranged by size: 2573 1156 1118 1012 499 21 BmgBI CAC'GTC Cuts at: 1318 2630 1318 Size: 1312 5067 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 4110 5068 442 Size: 416 53 83 399 24 2693 958 1753 Fragments arranged by size: 2693 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6379 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2633 2776 3002 3083 3522 5039 1239 Size: 1394 143 226 81 439 1517 2579 Fragments arranged by size: 2579 1517 1394 439 226 143 81 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 1995 2394 2835 2999 3057 3657 4504 5369 5667 Size: 399 441 164 58 600 847 865 298 Cuts at: 5667 1995 Size: 2707 Fragments arranged by size: 2707 865 847 600 441 399 298 164 58 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 5042 882 Size: 617 3543 2219 Fragments arranged by size: 3543 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 2116 4170 493 Size: 711 912 2054 2702 Fragments arranged by size: 2702 2054 912 711 BsaBI GATnn'nnATC Cuts at: 2439 6373 2439 Size: 3934 2445 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2546 4186 4568 5203 Size: 53 83 186 936 1013 1640 382 635 Cuts at: 5203 275 Size: 1451 Fragments arranged by size: 1640 1451 1013 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 4812 5210 5484 5631 6042 4812 Size: 398 274 147 411 5149 Fragments arranged by size: 5149 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 3081 3111 1178 Size: 30 165 30 1678 30 4446 Fragments arranged by size: 4446 1678 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2603 3467 1186 Size: 1417 864 4098 Fragments arranged by size: 4098 1417 864 BseYI C'CCAG_C Cuts at: 2094 2747 3352 5582 6078 2094 Size: 653 605 2230 496 2395 Fragments arranged by size: 2395 2230 653 605 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 3435 1163 Size: 573 1699 4107 Fragments arranged by size: 4107 1699 573 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3516 3829 4590 5618 1540 Size: 275 186 1515 313 761 1028 2301 Fragments arranged by size: 2301 1515 1028 761 313 275 186 BsiHKAI G_wGCw'C Cuts at: 2908 3325 3448 3654 4440 4525 5596 2908 Size: 417 123 206 786 85 1071 3691 Fragments arranged by size: 3691 1071 786 417 206 123 85 BsmI GAATG_Cn' Cuts at: 2828 3890 3983 2828 Size: 1062 93 5224 Fragments arranged by size: 5224 1062 93 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2908 2929 3325 3428 3448 3499 3654 Size: 399 1118 21 396 103 20 51 155 Cuts at: 3654 3698 4440 4525 5596 1391 Size: 44 742 85 1071 2174 Fragments arranged by size: 2174 1118 1071 742 399 396 155 103 85 51 44 21 20 BspEI T'CCGG_A Cuts at: 6042 6042 Size: 6379 BspHI T'CATG_A Cuts at: 4268 5998 4268 Size: 1730 4649 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3411 3734 844 Size: 2567 323 3489 Fragments arranged by size: 3489 2567 323 BsrBI CCG'CTC Cuts at: 1394 1823 4266 1394 Size: 429 2443 3507 Fragments arranged by size: 3507 2443 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2769 3375 4874 5048 65 Size: 1183 1521 606 1499 174 1396 Fragments arranged by size: 1521 1499 1396 1183 606 174 BsrFI r'CCGG_y Cuts at: 1700 1810 5023 5210 1700 Size: 110 3213 187 2869 Fragments arranged by size: 3213 2869 187 110 BsrGI T'GTAC_A Cuts at: 96 3594 96 Size: 3498 2881 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6379 BssSI C'ACGA_G Cuts at: 2556 3563 4439 5451 2556 Size: 1007 876 1012 3484 Fragments arranged by size: 3484 1012 1007 876 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 6379 BstBI TT'CG_AA Cuts at: 5183 5183 Size: 6379 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 6379 BstXI CCAn_nnnn'nTGG Cuts at: 3155 3155 Size: 6379 BstYI r'GATC_y Cuts at: 1972 2023 3709 4076 4461 4478 5930 6016 6028 Size: 51 1686 367 385 17 1452 86 12 Cuts at: 6028 6374 1972 Size: 346 1977 Fragments arranged by size: 1977 1686 1452 385 367 346 86 51 17 12 Bsu36I CC'TnA_GG Cuts at: 2138 2637 2138 Size: 499 5880 BtgI C'CryG_G Cuts at: 513 1065 2004 3051 3261 513 Size: 552 939 1047 210 3631 Fragments arranged by size: 3631 1047 939 552 210 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 2826 487 Size: 683 56 1600 4040 Fragments arranged by size: 4040 1600 683 56 BtsI GCAGTG_nn' Cuts at: 2969 3546 3895 4689 4709 2969 Size: 577 349 794 20 4639 Fragments arranged by size: 4639 794 577 349 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6379 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6344 DraI TTT'AAA Cuts at: 3683 3683 Size: 6379 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 3157 5386 809 Size: 507 1841 2229 1802 Fragments arranged by size: 2229 1841 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 3826 4715 6344 Size: 54 1425 50 275 18 1996 889 1629 Cuts at: 6344 8 Size: 43 Fragments arranged by size: 1996 1629 1425 889 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3826 1537 Size: 275 2014 4090 Fragments arranged by size: 4090 2014 275 EarI CTCTTCn'nnn_ Cuts at: 1353 4309 1353 Size: 2956 3423 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4966 5340 5486 387 Size: 1064 138 3377 374 146 1280 Fragments arranged by size: 3377 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 3446 3652 3446 Size: 206 6173 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2633 2711 2776 3002 3083 3128 3522 Size: 393 1001 78 65 226 81 45 394 Cuts at: 3522 4442 5039 5826 6094 1239 Size: 920 597 787 268 1524 Fragments arranged by size: 1524 1001 920 787 597 394 393 268 226 81 78 65 45 EcoNI CCTnn'n_nnAGG Cuts at: 2603 2603 Size: 6379 EcoO109I rG'GnC_Cy Cuts at: 2925 2926 3159 3170 3400 2925 Size: 1 233 11 230 5904 Fragments arranged by size: 5904 233 230 11 1 EcoRI G'AATT_C Cuts at: 2314 3688 4066 2314 Size: 1374 378 4627 Fragments arranged by size: 4627 1374 378 EcoRV GAT'ATC Cuts at: 3079 3079 Size: 6379 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2417 3040 2417 Size: 623 5756 FspI TGC'GCA Cuts at: 4885 4885 Size: 6379 HaeII r_GCGC'y Cuts at: 1678 5526 1678 Size: 3848 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2550 2582 2791 2823 3081 Size: 32 135 32 978 32 209 32 258 Cuts at: 3081 3113 5020 5052 5094 5126 1373 Size: 32 1907 32 42 32 2626 Fragments arranged by size: 2626 1907 978 258 209 135 42 32 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2210 2593 3723 3969 669 Size: 988 281 272 383 1130 246 3079 Fragments arranged by size: 3079 1130 988 383 281 272 246 HindIII A'AGCT_T Cuts at: 748 2057 2223 748 Size: 1309 166 4904 Fragments arranged by size: 4904 1309 166 HpaI GTT'AAC Cuts at: 3969 3969 Size: 6379 KpnI G_GTAC'C Cuts at: 3704 3704 Size: 6379 MfeI C'AATT_G Cuts at: 2202 3978 2202 Size: 1776 4603 MluI A'CGCG_T Cuts at: 6255 6255 Size: 6379 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2210 5493 5677 1305 Size: 905 3283 184 2007 Fragments arranged by size: 3283 2007 905 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 4557 Fragments arranged by size: 4557 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2502 2628 3536 4337 4696 4855 518 Size: 648 1336 126 908 801 359 159 2042 Fragments arranged by size: 2042 1336 908 801 648 359 159 126 MspA1I CmG'CkG Cuts at: 1606 2717 3001 3460 3795 4472 5620 5865 1606 Size: 1111 284 459 335 677 1148 245 2120 Fragments arranged by size: 2120 1148 1111 677 459 335 284 245 NcoI C'CATG_G Cuts at: 513 1065 2004 3051 513 Size: 552 939 1047 3841 Fragments arranged by size: 3841 1047 939 552 NdeI CA'TA_TG Cuts at: 387 2251 387 Size: 1864 4515 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6379 NotI GC'GGCC_GC Cuts at: 3826 3826 Size: 6379 NspI r_CATG'y Cuts at: 1132 1229 3737 5282 1132 Size: 97 2508 1545 2229 Fragments arranged by size: 2508 2229 1545 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 6379 PciI A'CATG_T Cuts at: 5278 5278 Size: 6379 PflMI CCAn_nnn'nTGG Cuts at: 1108 3364 1108 Size: 2256 4123 PfoI T'CCnGG_A Cuts at: 2127 2339 2910 3499 2127 Size: 212 571 589 5007 Fragments arranged by size: 5007 589 571 212 PmeI GTTT'AAAC Cuts at: 3683 3683 Size: 6379 PmlI CAC'GTG Cuts at: 2116 2116 Size: 6379 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3170 3202 4448 4480 5961 5993 3170 Size: 32 1246 32 1481 32 3556 Fragments arranged by size: 3556 1481 1246 32 32 32 PpuMI rG'GwC_Cy Cuts at: 3159 3170 3400 3159 Size: 11 230 6138 Fragments arranged by size: 6138 230 11 PshAI GACnn'nnGTC Cuts at: 1613 2106 3332 1613 Size: 493 1226 4660 Fragments arranged by size: 4660 1226 493 PsiI TTA'TAA Cuts at: 2831 3949 2831 Size: 1118 5261 PspOMI G'GGCC_C Cuts at: 2925 2925 Size: 6379 PstI C_TGCA'G Cuts at: 830 3408 3731 830 Size: 2578 323 3478 Fragments arranged by size: 3478 2578 323 PvuI CG_AT'CG Cuts at: 2001 3516 2001 Size: 1515 4864 PvuII CAG'CTG Cuts at: 2717 3001 3460 2717 Size: 284 459 5636 Fragments arranged by size: 5636 459 284 SacI G_AGCT'C Cuts at: 3448 3654 3448 Size: 206 6173 SalI G'TCGA_C Cuts at: 1936 3721 1936 Size: 1785 4594 SbfI CC_TGCA'GG Cuts at: 3731 3731 Size: 6379 ScaI AGT'ACT Cuts at: 4627 6339 4627 Size: 1712 4667 SfcI C'TryA_G Cuts at: 826 1045 2430 2718 3404 3727 4862 5543 5734 Size: 219 1385 288 686 323 1135 681 191 Cuts at: 5734 826 Size: 1471 Fragments arranged by size: 1471 1385 1135 686 681 323 288 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6379 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6379 SmaI CCC'GGG Cuts at: 1872 3706 1872 Size: 1834 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2010 2409 2850 3014 3072 3672 Size: 197 926 67 399 441 164 58 600 Cuts at: 3672 4483 5384 5646 820 Size: 811 901 262 1553 Fragments arranged by size: 1553 926 901 811 600 441 399 262 197 164 67 58 SnaBI TAC'GTA Cuts at: 493 4170 493 Size: 3677 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6379 SphI G_CATG'C Cuts at: 1132 3737 1132 Size: 2605 3774 SspI AAT'ATT Cuts at: 5 52 4303 5 Size: 47 4251 2081 Fragments arranged by size: 4251 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 6379 StyI C'CwwG_G Cuts at: 513 1065 2004 3051 3818 513 Size: 552 939 1047 767 3074 Fragments arranged by size: 3074 1047 939 767 552 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 4406 4423 4576 4761 5206 325 Size: 1255 2826 17 153 185 445 1498 Fragments arranged by size: 2826 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 3594 4625 6337 Size: 274 80 33 51 1443 1617 1031 1712 Cuts at: 6337 96 Size: 138 Fragments arranged by size: 1712 1617 1443 1031 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2335 2367 6339 6371 2335 Size: 32 3972 32 2343 Fragments arranged by size: 3972 2343 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 6379 XbaI T'CTAG_A Cuts at: 3715 3715 Size: 6379 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6379 XmaI C'CCGG_G Cuts at: 1870 3704 1870 Size: 1834 4545 XmnI GAAnn'nnTTC Cuts at: 4508 4508 Size: 6379 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 4187 276 Size: 53 83 186 936 2653 2468 Fragments arranged by size: 2653 2468 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI EcoRV FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PfoI PmeI PmlI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI PvuII SacI SalI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI AscI AvrII BaeI BbeI BbvCI BfrBI BplI Bpu10I BsiWI BsmBI BstZ17I DraIII FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PspXI PsrI RsrII SacII SanDI SapI SexAI SfiI SfoI SrfI SwaI XcmI