Restriction map for
pFN21AB6307
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# NO CUT ENZYMES ( 155 enzymes) #
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AatII Acc65I AccI AccIII AclI
AflII AflIII AgeI AhdI Alw44I
AocI Aor51HI ApaI ApaLI AscI
AseI AsnI Asp700I Asp718I AspEI
AspHI AspI AvaI AviII AvrII
BalI BamHI BbrPI BbsI BcgI
BciVI BclI BfrI BglI BglII
BlnI BpuAI BsaAI BsaBI BsaI
BseAI BseRI BsgI BsiHKAI BsiWI
BsmBI BsoBI Bsp1407I BspDI BspEI
BspHI BspLU11I BspMI BsrBI BsrDI
BsrFI BsrGI BssHII BssSI Bst1107I
BstAPI BstBI BstEII BstPI BstXI
BstZ17I Bsu36I Cfr10I ClaI CpoI
DraI DraIII DrdI EaeI EagI
Eam1105I EclXI Eco47III Eco52I Eco81I
EcoO65I EcoRI EcoRV EcoT14I EcoT22I
FbaI FseI FspI HincII HindII
HindIII HpaI KpnI MamI MfeI
MluI MluNI MroI MscI MseI
MslI MunI NaeI NcoI NdeI
NgoMIV NheI NlaIII NotI NruI
NsiI NspI NspV PacI PaeR7I
PflFI PinAI PmaCI PmeI PmlI
PshAI PshBI Psp1406I PstI PvuI
PvuII RcaI RsrII SacI SalI
SapI SbfI SexAI SfcI SfiI
SfuI SgrAI SmaI SmlI SnaBI
SpeI SphI Sse8387I SspBI SspI
StyI SwaI Tru9I Tth111I XbaI
XcmI XhoI XmaCI XmaI XmnI
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# ONE CUT ENZYMES ( 51 enzymes) #
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AcsI 325 GAATTT [GA]^AATT[CT]
AlwI 489 GGATC GGATC(4/5)
AlwNI 545 CAGTGCCTG CAG[ACGT][ACGT][ACGT]^CTG
ApoI 325 GAATTT [GA]^AATT[CT]
AvaII 18 GGACC G^G[AT]CC
BanII 393 GAGCCC G[GA]GC[CT]^C
BbeI 45 GGCGCC GGCGC^C
BbvCI 336 GCTGAGG CCTCAGC(-5/-2)
BfaI 472 CTAG C^TAG
BlpI 562 GCTAAGC GC^T[ACGT]AGC
BmyI 393 GAGCCC G[AGT]GC[ACT]^C
BpmI 716 CTCCAG CTGGAG(16/14)
Bpu1102I 562 GCTAAGC GC^T[ACGT]AGC
BsaWI 51 TCCGGT [AT]^CCGG[AT]
BsiEI 33 CGACCG CG[GA][CT]^CG
BsmAI 291 GAGAC GTCTC(1/5)
BsmI 665 GCATTC GAATGC(1/-1)
Bsp1286I 393 GAGCCC G[AGT]GC[ACT]^C
BsrI 544 CCAGT ACTGG(1/-1)
BstYI 489 GGATCT [GA]^GATC[CT]
CelII 562 GCTAAGC GC^T[ACGT]AGC
DpnII 490 GATC ^GATC
DraII 17 GGGACCC [GA]G^G[ACGT]CC[CT]
DsaI 260 CCGCGG C^C[GA][CT]GG
EarI 553 GAAGAG CTCTTC(1/4)
EcoNI 630 CCTCAAAGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO109I 17 GGGACCC [GA]G^G[ACGT]CC[CT]
HaeII 45 GGCGCC [GA]GCGC^[CT]
HphI 198 GGTGA GGTGA(8/7)
KasI 45 GGCGCC G^GCGCC
Ksp632I 553 GAAGAG CTCTTC(1/4)
KspI 260 CCGCGG CCGC^GG
MaeI 472 CTAG C^TAG
MaeII 413 ACGT A^CGT
MaeIII 305 GTGAC ^GT[ACGT]AC
MboI 490 GATC ^GATC
MflI 489 GGATCT [GA]^GATC[CT]
NarI 45 GGCGCC GG^CGCC
NdeII 490 GATC ^GATC
PflMI 740 CCAAGTCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PpuMI 17 GGGACCC [GA]G^G[AT]CC[CT]
SacII 260 CCGCGG CCGC^GG
Sau3AI 490 GATC ^GATC
ScaI 758 AGTACT AGT^ACT
SfoI 45 GGCGCC GGC^GCC
StuI 340 AGGCCT AGG^CCT
TfiI 438 GATTC G^A[AT]TC
Tsp45I 305 GTGAC ^GT[GC]AC
Tsp509I 326 AATT ^AATT
Van91I 740 CCAAGTCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XhoII 489 GGATCT [GA]^GATC[CT]
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# TWO CUT ENZYMES ( 9 enzymes) #
###################################
AcyI 45 GGCGCC G[GA]^CG[CT]C
145 GGCGTC
AfaI 231 GTAC GT^AC
759 GTAC
BcnI 106 CCGGG CC^[GC]GG
114 CCGGG
BsaHI 45 GGCGCC G[GA]^CG[CT]C
145 GGCGTC
Hin1I 45 GGCGCC G[GA]^CG[CT]C
145 GGCGTC
MspA1I 36 CCGCTG C[AC]G^C[GT]G
260 CCGCGG
NciI 106 CCGGG CC^[GC]GG
114 CCGGG
RsaI 231 GTAC GT^AC
759 GTAC
SfaNI 443 GATGC GCATC(5/9)
646 GATGC
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# 3 OR MORE CUT ENZYMES ( 43 enzymes) #
#########################################
AccII AciI AluI BanI BbvI
BsaJI BsiYI BslI BsmFI BssKI
BstF5I BstNI BstUI Cac8I CfoI
Cfr13I DdeI EcoRII FauI Fnu4HI
FokI HaeIII HapII HgaI HhaI
HinP1I HinfI HpaII ItaI MboII
MnlI MspI MvaI MvnI MwoI
NlaIV PleI Sau96I ScrFI TaqI
TseI TspRI TthHB8I