Restriction map for
pFN21AE0964
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# NO CUT ENZYMES ( 152 enzymes) #
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AatII AccI AccII AccIII AclI
AcyI AflII AgeI AhdI Alw44I
AocI ApaI ApaLI AscI AseI
AsnI AspEI AspI AvaI AviII
AvrII BalI BamHI BbeI BbrPI
BbvCI BcgI BclI BcnI BfrI
BglI BlnI BlpI Bpu1102I BsaAI
BsaHI BsaI BsaWI BseAI BsiEI
BsiWI BsmBI BsmI BsoBI Bsp1407I
BspDI BspEI BspHI BspMI BsrBI
BsrDI BsrFI BsrGI BssHII BssSI
Bst1107I BstAPI BstBI BstEII BstPI
BstUI BstXI BstZ17I Bsu36I CelII
Cfr10I ClaI CpoI DraIII DrdI
DsaI EaeI EagI Eam1105I EclXI
Eco52I Eco81I EcoO65I EcoRV EcoT14I
EcoT22I FauI FbaI FseI FspI
HapII HgaI Hin1I HindIII HpaI
HpaII KasI KspI MfeI MluI
MluNI MroI MscI MspA1I MspI
MunI MvnI MwoI NaeI NarI
NciI NcoI NgoMIV NheI NotI
NruI NsiI NspV PacI PaeR7I
PflFI PinAI PmaCI PmeI PmlI
PshAI PshBI Psp1406I PstI PvuI
PvuII RcaI RsrII SacII SalI
SapI SbfI ScaI SfiI SfoI
SfuI SgrAI SmaI SnaBI SpeI
Sse8387I SspBI SspI StuI StyI
SwaI Tth111I XbaI XcmI XhoI
XmaCI XmaI
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# ONE CUT ENZYMES ( 43 enzymes) #
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Acc65I 406 GGTACC G^GTACC
AciI 723 GCGG CCGC(-3/-1)
AflIII 171 ACATGT A^C[GA][CT]GT
Aor51HI 1105 AGCGCT AGC^GCT
Asp700I 481 GAATATGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
Asp718I 406 GGTACC G^GTACC
BanI 406 GGTACC G^G[CT][GA]CC
BbsI 1196 GAAGAC GAAGAC(2/6)
BciVI 440 GTATCC GTATCC(6/5)
BpmI 110 CTGGAG CTGGAG(16/14)
BpuAI 1196 GAAGAC GAAGAC(2/6)
BsaBI 1337 GATCTCCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BseRI 1141 GAGGAG GAGGAG(10/8)
BspLU11I 171 ACATGT A^CATGT
Cac8I 716 GCATGC GC[ACGT]^[ACGT]GC
CfoI 1106 GCGC GCG^C
DraI 412 TTTAAA TTT^AAA
DraII 764 AGGACCC [GA]G^G[ACGT]CC[CT]
EarI 1441 GAAGAG CTCTTC(1/4)
Eco47III 1105 AGCGCT AGC^GCT
EcoNI 616 CCTAGAAAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO109I 764 AGGACCC [GA]G^G[ACGT]CC[CT]
HaeII 1105 AGCGCT [GA]GCGC^[CT]
HhaI 1106 GCGC GCG^C
HinP1I 1106 GCGC G^CGC
HphI 493 GGTGA GGTGA(8/7)
KpnI 406 GGTACC GGTAC^C
Ksp632I 1441 GAAGAG CTCTTC(1/4)
MaeII 695 ACGT A^CGT
MamI 1337 GATCTCCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
NdeI 896 CATATG CA^TATG
PflMI 867 CCAATTTTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PpuMI 764 AGGACCC [GA]G^G[AT]CC[CT]
SacI 808 GAGCTC GAGCT^C
SexAI 539 ACCAGGT A^CC[AT]GGT
SfcI 570 CTACAG C^T[GA][CT]AG
SphI 716 GCATGC GCATG^C
TaqI 1079 TCGA T^CGA
TfiI 1027 GATTC G^A[AT]TC
Tsp45I 692 GTGAC ^GT[GC]AC
TthHB8I 1079 TCGA T^CGA
Van91I 867 CCAATTTTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XmnI 481 GAATATGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
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# TWO CUT ENZYMES ( 23 enzymes) #
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AlwNI 754 CAGAACCTG CAG[ACGT][ACGT][ACGT]^CTG
849 CAGTTGCTG
AspHI 808 GAGCTC G[AT]GC[AT]^C
881 GTGCTC
AvaII 765 GGACC G^G[AT]CC
961 GGACC
BanII 808 GAGCTC G[GA]GC[CT]^C
833 GGGCTC
BfaI 370 CTAG C^TAG
617 CTAG
BglII 389 AGATCT A^GATCT
1336 AGATCT
BsgI 778 GTGCAG GTGCAG(16/14)
1132 GTGCAG
BsiHKAI 808 GAGCTC G[AT]GC[AT]^C
881 GTGCTC
BsmAI 1444 GAGAC GTCTC(1/5)
1215 GTCTC
BsmFI 634 GGGAC GGGAC(10/14)
581 GTCCC
BssKI 540 CCAGG ^CC[ACGT]GG
746 CCTGG
BstNI 540 CCAGG CC^[AT]GG
746 CCTGG
EcoRI 296 GAATTC G^AATTC
328 GAATTC
EcoRII 540 CCAGG ^CC[AT]GG
746 CCTGG
HincII 420 GTCAAC GT[CT]^[GA]AC
1054 GTCAAC
HindII 420 GTCAAC GT[CT]^[GA]AC
1054 GTCAAC
MaeI 370 CTAG C^TAG
617 CTAG
MslI 472 CATGAAAATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
490 CATGGTGATG
MvaI 540 CCAGG CC^[AT]GG
746 CCTGG
NspI 171 ACATGT [GA]CATG^[CT]
716 GCATGC
PleI 1111 GAGTC GAGTC(4/5)
1213 GAGTC
ScrFI 540 CCAGG CC^[ACGT]GG
746 CCTGG
SmlI 925 CTCAAG C^T[CT][GA]AG
1217 CTCAAG
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# 3 OR MORE CUT ENZYMES ( 40 enzymes) #
#########################################
AcsI AfaI AluI AlwI ApoI
BbvI BmyI BsaJI BsiYI BslI
Bsp1286I BsrI BstF5I BstYI Cfr13I
DdeI DpnII Fnu4HI FokI HaeIII
HinfI ItaI MaeIII MboI MboII
MflI MnlI MseI NdeII NlaIII
NlaIV RsaI Sau3AI Sau96I SfaNI
Tru9I TseI Tsp509I TspRI XhoII