(Circular) (Six-base) MAPSORT of: pFN21AE0964.seq Check: 7651 from: 1 to: 6229 pFN21AE0964 6229 bp With 182 enzymes: SgfI * April 15, 2010 09:24 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 4039 278 Size: 53 83 186 936 2503 2468 Fragments arranged by size: 2503 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3572 1937 Size: 1635 4594 Acc65I G'GTAC_C Cuts at: 2411 3550 2411 Size: 1139 5090 AclI AA'CG_TT Cuts at: 1563 4356 4729 1563 Size: 2793 373 3063 Fragments arranged by size: 3063 2793 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2786 4292 5676 5944 1632 Size: 1154 1506 1384 268 1917 Fragments arranged by size: 1917 1506 1384 1154 268 AfeI AGC'GCT Cuts at: 3112 3112 Size: 6229 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 6032 AflIII A'CryG_T Cuts at: 1203 2176 5128 6105 1203 Size: 973 2952 977 1327 Fragments arranged by size: 2952 1327 977 973 AgeI A'CCGG_T Cuts at: 5060 5060 Size: 6229 AhdI GACnn_n'nnGTC Cuts at: 4958 4958 Size: 6229 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 6229 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 6229 AlwNI CAG_nnn'CTG Cuts at: 2764 2859 3972 5544 2764 Size: 95 1113 1572 3449 Fragments arranged by size: 3449 1572 1113 95 ApaLI G'TGCA_C Cuts at: 4286 5442 4286 Size: 1156 5073 ApoI r'AATT_y Cuts at: 1493 2228 2301 2333 2937 3538 3764 3916 6025 Size: 735 73 32 604 601 226 152 2109 Cuts at: 6025 1493 Size: 1697 Fragments arranged by size: 2109 1697 735 604 601 226 152 73 32 AseI AT'TA_AT Cuts at: 160 1032 4783 160 Size: 872 3751 1606 Fragments arranged by size: 3751 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6229 AvaI C'yCGr_G Cuts at: 1870 1943 3554 1870 Size: 73 1611 4545 Fragments arranged by size: 4545 1611 73 BamHI G'GATC_C Cuts at: 3559 3926 3559 Size: 367 5862 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2411 3550 5005 618 Size: 325 217 627 624 1139 1455 1842 Fragments arranged by size: 1842 1455 1139 627 624 325 217 BanII G_rGCy'C Cuts at: 1391 2817 2842 3548 1391 Size: 1426 25 706 4072 Fragments arranged by size: 4072 1426 706 25 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 3208 928 Size: 971 1309 3949 Fragments arranged by size: 3949 1309 971 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 1987 2021 4420 4454 1903 Size: 34 50 34 2399 34 3678 Fragments arranged by size: 3678 2399 50 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 2456 4121 5331 2456 Size: 1665 1210 3354 Fragments arranged by size: 3354 1665 1210 BclI T'GATC_A Cuts at: 1548 1548 Size: 6229 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4840 136 Size: 107 122 71 631 605 3168 1525 Fragments arranged by size: 3168 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 2394 3341 6224 2394 Size: 947 2883 2399 Fragments arranged by size: 2883 2399 947 BlpI GC'TnA_GC Cuts at: 3646 3646 Size: 6229 Bme1580I G_kGCm'C Cuts at: 1790 4290 5446 1790 Size: 2500 1156 2573 Fragments arranged by size: 2573 2500 1156 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 6229 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2105 2815 3424 Size: 416 53 83 399 24 688 710 609 Cuts at: 3424 3960 4918 442 Size: 536 958 1753 Fragments arranged by size: 1753 958 710 688 609 536 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6229 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2136 4889 1239 Size: 897 2753 2579 Fragments arranged by size: 2753 2579 897 Bpu10I CC'TnA_GC Cuts at: 3183 3183 Size: 6229 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2915 3207 4354 5219 5517 2915 Size: 292 1147 865 298 3627 Fragments arranged by size: 3627 1147 865 298 292 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4892 882 Size: 617 3393 2219 Fragments arranged by size: 3393 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 4020 493 Size: 711 2816 2702 Fragments arranged by size: 2816 2702 711 BsaBI GATnn'nnATC Cuts at: 3346 6223 3346 Size: 2877 3352 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 4036 4418 5053 275 Size: 53 83 186 936 2503 382 635 1451 Fragments arranged by size: 2503 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 4662 5060 5334 5481 5892 4662 Size: 398 274 147 411 4999 Fragments arranged by size: 4999 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 6004 Fragments arranged by size: 6004 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 3161 1186 Size: 1975 4254 BseYI C'CCAG_C Cuts at: 5432 5928 5432 Size: 496 5733 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2804 3158 1163 Size: 573 1068 354 4234 Fragments arranged by size: 4234 1068 573 354 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3679 4440 5468 1540 Size: 275 186 1678 761 1028 2301 Fragments arranged by size: 2301 1678 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2817 2890 4290 4375 5446 2817 Size: 73 1400 85 1071 3600 Fragments arranged by size: 3600 1400 1071 85 73 BsmI GAATG_Cn' Cuts at: 3740 3833 3740 Size: 93 6136 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2817 2842 2890 3548 4290 4375 5446 Size: 399 1027 25 48 658 742 85 1071 Cuts at: 5446 1391 Size: 2174 Fragments arranged by size: 2174 1071 1027 742 658 399 85 48 25 BspEI T'CCGG_A Cuts at: 5892 5892 Size: 6229 BspHI T'CATG_A Cuts at: 4118 5848 4118 Size: 1730 4499 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3584 844 Size: 2740 3489 BsrBI CCG'CTC Cuts at: 1394 1823 4116 1394 Size: 429 2293 3507 Fragments arranged by size: 3507 2293 429 BsrDI GCAATG_nn' Cuts at: 65 1248 4724 4898 65 Size: 1183 3476 174 1396 Fragments arranged by size: 3476 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 4873 5060 1700 Size: 110 3063 187 2869 Fragments arranged by size: 3063 2869 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 6229 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6229 BssSI C'ACGA_G Cuts at: 4289 5301 4289 Size: 1012 5217 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 6229 BstBI TT'CG_AA Cuts at: 5033 5033 Size: 6229 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 6229 BstYI r'GATC_y Cuts at: 1972 2318 2394 2706 3341 3559 3926 4311 4328 Size: 346 76 312 635 218 367 385 17 Cuts at: 4328 5780 5866 5878 6224 1972 Size: 1452 86 12 346 1977 Fragments arranged by size: 1977 1452 635 385 367 346 346 312 218 86 76 17 12 BtgI C'CryG_G Cuts at: 513 1065 513 Size: 552 5677 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5490 Fragments arranged by size: 5490 683 56 BtsI GCAGTG_nn' Cuts at: 3745 4539 4559 3745 Size: 794 20 5415 Fragments arranged by size: 5415 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6229 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6194 DraI TTT'AAA Cuts at: 2419 3533 2419 Size: 1114 5115 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5236 809 Size: 507 3920 1802 Fragments arranged by size: 3920 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 3676 4565 6194 Size: 54 1425 50 275 18 1846 889 1629 Cuts at: 6194 8 Size: 43 Fragments arranged by size: 1846 1629 1425 889 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3676 1537 Size: 275 1864 4090 Fragments arranged by size: 4090 1864 275 EarI CTCTTCn'nnn_ Cuts at: 1353 3441 4159 1353 Size: 2088 718 3423 Fragments arranged by size: 3423 2088 718 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4816 5190 5336 387 Size: 1064 138 3227 374 146 1280 Fragments arranged by size: 3227 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 2815 2815 Size: 6229 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2136 2786 4292 4889 5676 5944 1239 Size: 393 504 650 1506 597 787 268 1524 Fragments arranged by size: 1524 1506 787 650 597 504 393 268 EcoNI CCTnn'n_nnAGG Cuts at: 2625 2625 Size: 6229 EcoO109I rG'GnC_Cy Cuts at: 2770 2770 Size: 6229 EcoRI G'AATT_C Cuts at: 2301 2333 3538 3916 2301 Size: 32 1205 378 4614 Fragments arranged by size: 4614 1205 378 32 FspI TGC'GCA Cuts at: 4735 4735 Size: 6229 HaeII r_GCGC'y Cuts at: 1678 3114 5376 1678 Size: 1436 2262 2531 Fragments arranged by size: 2531 2262 1436 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 4870 4902 4944 4976 1373 Size: 32 135 32 3298 32 42 32 2626 Fragments arranged by size: 3298 2626 135 42 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2427 3061 3573 3819 669 Size: 988 281 489 634 512 246 3079 Fragments arranged by size: 3079 988 634 512 489 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 6229 HpaI GTT'AAC Cuts at: 3819 3819 Size: 6229 KpnI G_GTAC'C Cuts at: 2415 3554 2415 Size: 1139 5090 MfeI C'AATT_G Cuts at: 3828 3828 Size: 6229 MluI A'CGCG_T Cuts at: 6105 6105 Size: 6229 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2436 2787 5343 5527 1305 Size: 1131 351 2556 184 2007 Fragments arranged by size: 2556 2007 1131 351 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 4407 Fragments arranged by size: 4407 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2481 2499 4187 4546 4705 518 Size: 648 1315 18 1688 359 159 2042 Fragments arranged by size: 2042 1688 1315 648 359 159 18 MspA1I CmG'CkG Cuts at: 1606 3645 4322 5470 5715 1606 Size: 2039 677 1148 245 2120 Fragments arranged by size: 2120 2039 1148 677 245 NcoI C'CATG_G Cuts at: 513 1065 513 Size: 552 5677 NdeI CA'TA_TG Cuts at: 387 2902 387 Size: 2515 3714 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6229 NotI GC'GGCC_GC Cuts at: 3676 3676 Size: 6229 NspI r_CATG'y Cuts at: 1132 1229 2180 2725 3587 5132 1132 Size: 97 951 545 862 1545 2229 Fragments arranged by size: 2229 1545 951 862 545 97 PasI CC'CwG_GG Cuts at: 1348 2751 1348 Size: 1403 4826 PciI A'CATG_T Cuts at: 2176 5128 2176 Size: 2952 3277 PflMI CCAn_nnn'nTGG Cuts at: 1108 2878 1108 Size: 1770 4459 PmeI GTTT'AAAC Cuts at: 3533 3533 Size: 6229 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4298 4330 5811 5843 4298 Size: 32 1481 32 4684 Fragments arranged by size: 4684 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2770 2770 Size: 6229 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6229 PsiI TTA'TAA Cuts at: 3799 3799 Size: 6229 PstI C_TGCA'G Cuts at: 830 3581 830 Size: 2751 3478 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6229 SacI G_AGCT'C Cuts at: 2817 2817 Size: 6229 SalI G'TCGA_C Cuts at: 1936 3571 1936 Size: 1635 4594 SbfI CC_TGCA'GG Cuts at: 3581 3581 Size: 6229 ScaI AGT'ACT Cuts at: 4477 6189 4477 Size: 1712 4517 SexAI A'CCwGG_T Cuts at: 2544 2544 Size: 6229 SfcI C'TryA_G Cuts at: 826 1045 2575 3577 4712 5393 5584 826 Size: 219 1530 1002 1135 681 191 1471 Fragments arranged by size: 1530 1471 1135 1002 681 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6229 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6229 SmaI CCC'GGG Cuts at: 1872 3556 1872 Size: 1684 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2930 3222 4333 5234 5496 820 Size: 197 926 987 292 1111 901 262 1553 Fragments arranged by size: 1553 1111 987 926 901 292 262 197 SnaBI TAC'GTA Cuts at: 493 4020 493 Size: 3527 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6229 SphI G_CATG'C Cuts at: 1132 2725 3587 1132 Size: 1593 862 3774 Fragments arranged by size: 3774 1593 862 SspI AAT'ATT Cuts at: 5 52 4153 5 Size: 47 4101 2081 Fragments arranged by size: 4101 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 6229 StyI C'CwwG_G Cuts at: 513 1065 3668 513 Size: 552 2603 3074 Fragments arranged by size: 3074 2603 552 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 4256 4273 4426 4611 5056 325 Size: 1255 2676 17 153 185 445 1498 Fragments arranged by size: 2676 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2461 4475 6187 Size: 274 80 33 51 1443 484 2014 1712 Cuts at: 6187 96 Size: 138 Fragments arranged by size: 2014 1712 1443 484 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 6189 6221 6189 Size: 32 6197 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 6229 XbaI T'CTAG_A Cuts at: 3565 3565 Size: 6229 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6229 XmaI C'CCGG_G Cuts at: 1870 3554 1870 Size: 1684 4545 XmnI GAAnn'nnTTC Cuts at: 2490 4358 2490 Size: 1868 4361 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 4037 276 Size: 53 83 186 936 2503 2468 Fragments arranged by size: 2503 2468 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AfeI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PmeI PpiI PpuMI PshAI PsiI PstI PvuI SacI SalI SbfI ScaI SexAI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AloI ApaI AscI AvrII BaeI BbeI BbvCI BfrBI BplI BsiWI BsmBI BstXI BstZ17I Bsu36I DraIII EcoRV FalI FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PfoI PmlI PspOMI PspXI PsrI PvuII RsrII SacII SanDI SapI SfiI SfoI SrfI SwaI XcmI