Restriction map for
pFN21AE0964
(Linear) (Six-base) MAPSORT of: KIEE0964.seq Check: 5760 from: 1 to: 1524
KIEE0964.orf
With 182 enzymes: SgfI *
April 24, 2009 13:19 ..
Acc65I G'GTAC_C
Cuts at: 0 406 1524
Size: 406 1118
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 781 1524
Size: 781 743
AfeI AGC'GCT
Cuts at: 0 1107 1524
Size: 1107 417
AflIII A'CryG_T
Cuts at: 0 171 1524
Size: 171 1353
AlwNI CAG_nnn'CTG
Cuts at: 0 759 854 1524
Size: 759 95 670
Fragments arranged by size:
759 670 95
ApoI r'AATT_y
Cuts at: 0 223 296 328 932 1524
Size: 223 73 32 604 592
Fragments arranged by size:
604 592 223 73 32
BanI G'GyrC_C
Cuts at: 0 406 1524
Size: 406 1118
BanII G_rGCy'C
Cuts at: 0 812 837 1524
Size: 812 25 687
Fragments arranged by size:
812 687 25
BbsI GAAGACnn'nnnn_
Cuts at: 0 1203 1524
Size: 1203 321
BciVI GTATCCnnnnn_n'
Cuts at: 0 451 1524
Size: 451 1073
BglII A'GATC_T
Cuts at: 0 389 1336 1524
Size: 389 947 188
Fragments arranged by size:
947 389 188
BmrI ACTGGGnnnn_n'
Cuts at: 0 100 810 1419 1524
Size: 100 710 609 105
Fragments arranged by size:
710 609 105 100
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 131 1524
Size: 131 1393
Bpu10I CC'TnA_GC
Cuts at: 0 1178 1524
Size: 1178 346
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 910 1202 1524
Size: 910 292 322
Fragments arranged by size:
910 322 292
BsaBI GATnn'nnATC
Cuts at: 0 1341 1524
Size: 1341 183
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 1156 1524
Size: 1156 368
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 799 1153 1524
Size: 799 354 371
Fragments arranged by size:
799 371 354
BsiHKAI G_wGCw'C
Cuts at: 0 812 885 1524
Size: 812 73 639
Fragments arranged by size:
812 639 73
Bsp1286I G_dGCh'C
Cuts at: 0 812 837 885 1524
Size: 812 25 48 639
Fragments arranged by size:
812 639 48 25
BstYI r'GATC_y
Cuts at: 0 313 389 701 1336 1524
Size: 313 76 312 635 188
Fragments arranged by size:
635 313 312 188 76
DraI TTT'AAA
Cuts at: 0 414 1524
Size: 414 1110
EarI CTCTTCn'nnn_
Cuts at: 0 1436 1524
Size: 1436 88
EcoICRI GAG'CTC
Cuts at: 0 810 1524
Size: 810 714
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 131 781 1524
Size: 131 650 743
Fragments arranged by size:
743 650 131
EcoNI CCTnn'n_nnAGG
Cuts at: 0 620 1524
Size: 620 904
EcoO109I rG'GnC_Cy
Cuts at: 0 765 1524
Size: 765 759
EcoRI G'AATT_C
Cuts at: 0 296 328 1524
Size: 296 32 1196
Fragments arranged by size:
1196 296 32
HaeII r_GCGC'y
Cuts at: 0 1109 1524
Size: 1109 415
HincII GTy'rAC
Cuts at: 0 422 1056 1524
Size: 422 634 468
Fragments arranged by size:
634 468 422
KpnI G_GTAC'C
Cuts at: 0 410 1524
Size: 410 1114
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 431 782 1524
Size: 431 351 742
Fragments arranged by size:
742 431 351
MslI CAynn'nnrTG
Cuts at: 0 476 494 1524
Size: 476 18 1030
Fragments arranged by size:
1030 476 18
NdeI CA'TA_TG
Cuts at: 0 897 1524
Size: 897 627
NspI r_CATG'y
Cuts at: 0 175 720 1524
Size: 175 545 804
Fragments arranged by size:
804 545 175
PasI CC'CwG_GG
Cuts at: 0 746 1524
Size: 746 778
PciI A'CATG_T
Cuts at: 0 171 1524
Size: 171 1353
PflMI CCAn_nnn'nTGG
Cuts at: 0 873 1524
Size: 873 651
PpuMI rG'GwC_Cy
Cuts at: 0 765 1524
Size: 765 759
SacI G_AGCT'C
Cuts at: 0 812 1524
Size: 812 712
SexAI A'CCwGG_T
Cuts at: 0 539 1524
Size: 539 985
SfcI C'TryA_G
Cuts at: 0 570 1524
Size: 570 954
SmlI C'TyrA_G
Cuts at: 0 925 1217 1524
Size: 925 292 307
Fragments arranged by size:
925 307 292
SphI G_CATG'C
Cuts at: 0 720 1524
Size: 720 804
TatI w'GTAC_w
Cuts at: 0 456 1524
Size: 456 1068
XmnI GAAnn'nnTTC
Cuts at: 0 485 1524
Size: 485 1039
Enzymes that do cut:
Acc65I AcuI AfeI AflIII AlwNI ApoI BanI BanII
BbsI BciVI BglII BmrI BpmI Bpu10I BpuEI BsaBI
BseRI BsgI BsiHKAI Bsp1286I BstYI DraI EarI EcoICRI
Eco57MI EcoNI EcoO109I EcoRI HaeII HincII KpnI MmeI
MslI NdeI NspI PasI PciI PflMI PpuMI SacI
SexAI SfcI SmlI SphI TatI XmnI
Enzymes that do not cut:
AarI AatII AccI AclI AflII AgeI AhdI AleI
AlfI AloI ApaI ApaLI AscI AseI AsiSI AvaI
AvrII BaeI BamHI BbeI BbvCI BcgI BclI BfrBI
BglI BlpI Bme1580I BmgBI BmtI BplI BsaI BsaAI
BsaHI BsaWI BsaXI BseYI BsiEI BsiWI BsmI BsmBI
BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII
BssSI BstAPI BstBI BstEII BstXI BstZ17I Bsu36I BtgI
BtgZI BtsI ClaI CspCI DraIII DrdI EaeI EagI
EciI EcoRV FalI FseI FspI FspAI Hin4I HindIII
HpaI KasI MfeI MluI MscI MspA1I NaeI NarI
NcoI NgoMIV NheI NotI NruI NsiI PacI PfoI
PmeI PmlI PpiI PshAI PsiI PspOMI PspXI PsrI
PstI PvuI PvuII RsrII SacII SalI SanDI SapI
SbfI ScaI SfiI SfoI SgfI SgrAI SmaI SnaBI
SpeI SrfI SspI StuI StyI SwaI TaqII TstI
Tth111I XbaI XcmI XhoI XmaI ZraI