Restriction map for
pFN21AE4090
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# NO CUT ENZYMES ( 128 enzymes) #
##################################
AccIII AclI AcsI AflII AflIII
AgeI AhdI Alw44I AlwI AlwNI
AocI Aor51HI ApaI ApaLI ApoI
AscI AseI AsnI Asp700I AspEI
AspI AviII AvrII BamHI BbeI
BbvCI BcgI BciVI BclI BfrI
BglI BglII BlnI BlpI Bpu1102I
BsaBI BsaI BseAI BsgI BsiWI
BsmI Bsp1407I BspEI BspHI BspLU11I
BspMI BsrGI BssSI Bst1107I BstAPI
BstBI BstYI BstZ17I Bsu36I CelII
CpoI DpnII DraI DraIII DrdI
Eam1105I Eco47III Eco81I EcoRI EcoRV
EcoT14I EcoT22I FbaI FseI FspI
HindIII HpaI KasI MamI MboI
MfeI MflI MluI MroI MseI
MunI NarI NcoI NdeI NdeII
NheI NruI NsiI NspV PacI
PflFI PflMI PinAI PmeI PshAI
PshBI Psp1406I PvuI RcaI RsrII
SalI SapI Sau3AI SbfI ScaI
SfiI SfoI SfuI SgrAI SmaI
SnaBI SpeI SphI Sse8387I SspBI
SspI StuI StyI SwaI TfiI
Tru9I Tth111I Van91I XbaI XhoII
XmaCI XmaI XmnI
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# ONE CUT ENZYMES ( 33 enzymes) #
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AatII 259 GACGTC GACGT^C
Acc65I 1308 GGTACC G^GTACC
AccI 988 GTCTAC GT^[AC][GT]AC
Asp718I 1308 GGTACC G^GTACC
BalI 821 TGGCCA TGG^CCA
BbrPI 247 CACGTG CAC^GTG
BsaAI 247 CACGTG [CT]AC^GT[GA]
BsaWI 220 TCCGGT [AT]^CCGG[AT]
BseRI 940 CTCCTC GAGGAG(10/8)
BspDI 733 ATCGAT AT^CGAT
BsrDI 1137 CATTGC GCAATG(2/0)
BstEII 1356 GGTCACC G^GT[ACGT]ACC
BstPI 1356 GGTCACC G^GT[ACGT]ACC
ClaI 733 ATCGAT AT^CGAT
EcoO65I 1356 GGTCACC G^GT[ACGT]ACC
HincII 1003 GTTGAC GT[CT]^[GA]AC
HindII 1003 GTTGAC GT[CT]^[GA]AC
KpnI 1308 GGTACC GGTAC^C
MluNI 821 TGGCCA TGG^CCA
MscI 821 TGGCCA TGG^CCA
NotI 134 GCGGCCGC GC^GGCCGC
NspI 1250 ACATGC [GA]CATG^[CT]
PaeR7I 484 CTCGAG C^TCGAG
PmaCI 247 CACGTG CAC^GTG
PmlI 247 CACGTG CAC^GTG
PpuMI 119 GGGACCC [GA]G^G[AT]CC[CT]
PstI 536 CTGCAG CTGCA^G
PvuII 382 CAGCTG CAG^CTG
SacI 411 GAGCTC GAGCT^C
SexAI 1352 ACCTGGT A^CC[AT]GGT
SmlI 484 CTCGAG C^T[CT][GA]AG
Tsp509I 1186 AATT ^AATT
XhoI 484 CTCGAG C^TCGAG
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# TWO CUT ENZYMES ( 26 enzymes) #
###################################
AcyI 196 GACGCC G[GA]^CG[CT]C
259 GACGTC
AfaI 676 GTAC GT^AC
1309 GTAC
AvaII 120 GGACC G^G[AT]CC
969 GGTCC
BanII 411 GAGCTC G[GA]GC[CT]^C
1122 GGGCTC
BfaI 115 CTAG C^TAG
1028 CTAG
BpmI 576 CTCCAG CTGGAG(16/14)
1270 CTGGAG
BsaHI 196 GACGCC G[GA]^CG[CT]C
259 GACGTC
BsmFI 119 GGGAC GGGAC(10/14)
873 GTCCC
DraII 119 GGGACCC [GA]G^G[ACGT]CC[CT]
887 AGGGCCT
DsaI 7 CCGCGG C^C[GA][CT]GG
132 CCGCGG
EagI 135 CGGCCG C^GGCCG
238 CGGCCG
EclXI 135 CGGCCG C^GGCCG
238 CGGCCG
Eco52I 135 CGGCCG C^GGCCG
238 CGGCCG
EcoNI 267 CCTGGAAGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
315 CCTGCGCGAGG
EcoO109I 119 GGGACCC [GA]G^G[ACGT]CC[CT]
887 AGGGCCT
HaeII 350 AGCGCC [GA]GCGC^[CT]
982 AGCGCC
Hin1I 196 GACGCC G[GA]^CG[CT]C
259 GACGTC
KspI 7 CCGCGG CCGC^GG
132 CCGCGG
MaeI 115 CTAG C^TAG
1028 CTAG
MslI 960 CATCGGTGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
1359 CACCGAAGTG
NaeI 240 GCCGGC GCC^GGC
547 GCCGGC
NgoMIV 240 GCCGGC G^CCGGC
547 GCCGGC
RsaI 676 GTAC GT^AC
1309 GTAC
SacII 7 CCGCGG CCGC^GG
132 CCGCGG
SfaNI 860 GCATC GCATC(5/9)
1031 GCATC
SfcI 536 CTGCAG C^T[GA][CT]AG
1070 CTACAG
#########################################
# 3 OR MORE CUT ENZYMES ( 71 enzymes) #
#########################################
AccII AciI AluI AspHI AvaI
BanI BbsI BbvI BcnI BmyI
BpuAI BsaJI BsiEI BsiHKAI BsiYI
BslI BsmAI BsmBI BsoBI Bsp1286I
BsrBI BsrFI BsrI BssHII BssKI
BstF5I BstNI BstUI BstXI Cac8I
CfoI Cfr10I Cfr13I DdeI EaeI
EarI EcoRII FauI Fnu4HI FokI
HaeIII HapII HgaI HhaI HinP1I
HinfI HpaII HphI ItaI Ksp632I
MaeII MaeIII MboII MnlI MspA1I
MspI MvaI MvnI MwoI NciI
NlaIII NlaIV PleI Sau96I ScrFI
TaqI TseI Tsp45I TspRI TthHB8I
XcmI