(Linear) (Six-base) MAPSORT of: KIEE4090.seq Check: 871 from: 1 to: 1368 KIEE4090.orf With 182 enzymes: SgfI * July 3, 2012 18:33 .. AatII G_ACGT'C Cuts at: 0 263 1368 Size: 263 1105 AccI GT'mk_AC Cuts at: 0 989 1368 Size: 989 379 Acc65I G'GTAC_C Cuts at: 0 1308 1368 Size: 1308 60 AleI CACnn'nnGTG Cuts at: 0 1363 1368 Size: 1363 5 AvaI C'yCGr_G Cuts at: 0 346 406 484 1368 Size: 346 60 78 884 Fragments arranged by size: 884 346 78 60 BanI G'GyrC_C Cuts at: 0 11 179 339 772 1092 1308 1335 1368 Size: 11 168 160 433 320 216 27 33 Fragments arranged by size: 433 320 216 168 160 33 27 11 BanII G_rGCy'C Cuts at: 0 415 1126 1368 Size: 415 711 242 Fragments arranged by size: 711 415 242 BbsI GAAGACnn'nnnn_ Cuts at: 0 255 468 846 1368 Size: 255 213 378 522 Fragments arranged by size: 522 378 255 213 Bme1580I G_kGCm'C Cuts at: 0 16 775 1172 1338 1368 Size: 16 759 397 166 30 Fragments arranged by size: 759 397 166 30 16 BmrI ACTGGGnnnn_n' Cuts at: 0 778 1368 Size: 778 590 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 561 1291 1368 Size: 561 730 77 Fragments arranged by size: 730 561 77 Bpu10I CC'TnA_GC Cuts at: 0 1313 1368 Size: 1313 55 BsaAI yAC'GTr Cuts at: 0 249 1368 Size: 249 1119 BsaHI Gr'CG_yC Cuts at: 0 197 260 1368 Size: 197 63 1108 Fragments arranged by size: 1108 197 63 BsaWI w'CCGG_w Cuts at: 0 220 1368 Size: 220 1148 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 0 25 628 658 1368 Size: 25 603 30 710 Fragments arranged by size: 710 603 30 25 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 0 931 1368 Size: 931 437 BseYI C'CCAG_C Cuts at: 0 90 123 187 291 933 1228 1368 Size: 90 33 64 104 642 295 140 Fragments arranged by size: 642 295 140 104 90 64 33 BsiEI CG_ry'CG Cuts at: 0 138 177 241 513 1368 Size: 138 39 64 272 855 Fragments arranged by size: 855 272 138 64 39 BsiHKAI G_wGCw'C Cuts at: 0 50 206 415 434 1368 Size: 50 156 209 19 934 Fragments arranged by size: 934 209 156 50 19 BsmBI CGTCTCn'nnnn_ Cuts at: 0 67 468 481 631 1368 Size: 67 401 13 150 737 Fragments arranged by size: 737 401 150 67 13 Bsp1286I G_dGCh'C Cuts at: 0 16 50 206 415 434 775 1126 1172 Size: 16 34 156 209 19 341 351 46 Cuts at: 1172 1338 1368 Size: 166 30 Fragments arranged by size: 351 341 209 166 156 46 34 30 19 16 BsrBI CCG'CTC Cuts at: 0 39 255 562 1368 Size: 39 216 307 806 Fragments arranged by size: 806 307 216 39 BsrDI GCAATG_nn' Cuts at: 0 1136 1368 Size: 1136 232 BsrFI r'CCGG_y Cuts at: 0 176 182 240 547 1368 Size: 176 6 58 307 821 Fragments arranged by size: 821 307 176 58 6 BssHII G'CGCG_C Cuts at: 0 82 127 154 424 1368 Size: 82 45 27 270 944 Fragments arranged by size: 944 270 82 45 27 BstEII G'GTnAC_C Cuts at: 0 1356 1368 Size: 1356 12 BstXI CCAn_nnnn'nTGG Cuts at: 0 223 589 819 1368 Size: 223 366 230 549 Fragments arranged by size: 549 366 230 223 BtgI C'CryG_G Cuts at: 0 7 132 1368 Size: 7 125 1236 Fragments arranged by size: 1236 125 7 ClaI AT'CG_AT Cuts at: 0 734 1368 Size: 734 634 EaeI y'GGCC_r Cuts at: 0 56 135 238 821 1368 Size: 56 79 103 583 547 Fragments arranged by size: 583 547 103 79 56 EagI C'GGCC_G Cuts at: 0 135 238 1368 Size: 135 103 1130 Fragments arranged by size: 1130 135 103 EarI CTCTTCn'nnn_ Cuts at: 0 266 961 1217 1368 Size: 266 695 256 151 Fragments arranged by size: 695 266 256 151 EcoICRI GAG'CTC Cuts at: 0 413 1368 Size: 413 955 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 0 561 1291 1368 Size: 561 730 77 Fragments arranged by size: 730 561 77 EcoNI CCTnn'n_nnAGG Cuts at: 0 271 319 1368 Size: 271 48 1049 Fragments arranged by size: 1049 271 48 EcoO109I rG'GnC_Cy Cuts at: 0 120 888 1368 Size: 120 768 480 Fragments arranged by size: 768 480 120 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 0 723 1368 Size: 723 645 HaeII r_GCGC'y Cuts at: 0 354 986 1368 Size: 354 632 382 Fragments arranged by size: 632 382 354 HincII GTy'rAC Cuts at: 0 1005 1368 Size: 1005 363 KpnI G_GTAC'C Cuts at: 0 1312 1368 Size: 1312 56 MscI TGG'CCA Cuts at: 0 823 1368 Size: 823 545 MslI CAynn'nnrTG Cuts at: 0 964 1363 1368 Size: 964 399 5 Fragments arranged by size: 964 399 5 MspA1I CmG'CkG Cuts at: 0 9 134 384 567 1368 Size: 9 125 250 183 801 Fragments arranged by size: 801 250 183 125 9 NaeI GCC'GGC Cuts at: 0 242 549 1368 Size: 242 307 819 Fragments arranged by size: 819 307 242 NgoMIV G'CCGG_C Cuts at: 0 240 547 1368 Size: 240 307 821 Fragments arranged by size: 821 307 240 NotI GC'GGCC_GC Cuts at: 0 135 1368 Size: 135 1233 NspI r_CATG'y Cuts at: 0 1254 1368 Size: 1254 114 PasI CC'CwG_GG Cuts at: 0 1342 1368 Size: 1342 26 PfoI T'CCnGG_A Cuts at: 0 101 266 1368 Size: 101 165 1102 Fragments arranged by size: 1102 165 101 PmlI CAC'GTG Cuts at: 0 249 1368 Size: 249 1119 PpuMI rG'GwC_Cy Cuts at: 0 120 1368 Size: 120 1248 PstI C_TGCA'G Cuts at: 0 540 1368 Size: 540 828 PvuII CAG'CTG Cuts at: 0 384 1368 Size: 384 984 SacI G_AGCT'C Cuts at: 0 415 1368 Size: 415 953 SacII CC_GC'GG Cuts at: 0 10 135 1368 Size: 10 125 1233 Fragments arranged by size: 1233 125 10 SanDI GG'GwC_CC Cuts at: 0 120 1368 Size: 120 1248 SexAI A'CCwGG_T Cuts at: 0 1352 1368 Size: 1352 16 SfcI C'TryA_G Cuts at: 0 536 1070 1368 Size: 536 534 298 Fragments arranged by size: 536 534 298 SmlI C'TyrA_G Cuts at: 0 484 1368 Size: 484 884 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 0 1096 1368 Size: 1096 272 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 0 27 628 660 809 841 1368 Size: 27 601 32 149 32 527 Fragments arranged by size: 601 527 149 32 32 27 XcmI CCAnnnn_n'nnnnTGG Cuts at: 0 930 1290 1328 1368 Size: 930 360 38 40 Fragments arranged by size: 930 360 40 38 XhoI C'TCGA_G Cuts at: 0 484 1368 Size: 484 884 ZraI GAC'GTC Cuts at: 0 261 1368 Size: 261 1107 Enzymes that do cut: AatII AccI Acc65I AleI AvaI BanI BanII BbsI Bme1580I BmrI BpmI Bpu10I BsaAI BsaHI BsaWI BsaXI BseRI BseYI BsiEI BsiHKAI BsmBI Bsp1286I BsrBI BsrDI BsrFI BssHII BstEII BstXI BtgI ClaI EaeI EagI EarI EcoICRI Eco57MI EcoNI EcoO109I FalI HaeII HincII KpnI MscI MslI MspA1I NaeI NgoMIV NotI NspI PasI PfoI PmlI PpuMI PstI PvuII SacI SacII SanDI SexAI SfcI SmlI TaqII TstI XcmI XhoI ZraI Enzymes that do not cut: AarI AclI AcuI AfeI AflII AflIII AgeI AhdI AlfI AloI AlwNI ApaI ApaLI ApoI AscI AseI AsiSI AvrII BaeI BamHI BbeI BbvCI BcgI BciVI BclI BfrBI BglI BglII BlpI BmgBI BmtI BplI BpuEI BsaI BsaBI BsgI BsiWI BsmI BspEI BspHI BspMI BsrGI BssSI BstAPI BstBI BstYI BstZ17I Bsu36I BtgZI BtsI CspCI DraI DraIII DrdI EciI EcoRI EcoRV FseI FspI FspAI Hin4I HindIII HpaI KasI MfeI MluI MmeI NarI NcoI NdeI NheI NruI NsiI PacI PciI PflMI PmeI PpiI PshAI PsiI PspOMI PspXI PsrI PvuI RsrII SalI SapI SbfI ScaI SfiI SfoI SgfI SgrAI SmaI SnaBI SpeI SphI SrfI SspI StuI StyI SwaI TatI Tth111I XbaI XmaI XmnI