################################## # NO CUT ENZYMES ( 118 enzymes) # ################################## Acc65I AccI AccIII AclI AcsI AflII AhdI Alw44I AlwNI AocI Aor51HI ApaLI ApoI AseI AsnI Asp700I Asp718I AspEI AviII AvrII BalI BamHI BbsI BbvCI BcgI BciVI BclI BfrI BglII BlnI BpuAI BsaBI BseAI BsmI Bsp1407I BspDI BspEI BspHI BspLU11I BspMI BsrDI BsrGI BsrI Bst1107I BstAPI BstBI BstEII BstF5I BstPI BstXI BstYI BstZ17I Bsu36I ClaI CpoI DraI DraIII DrdI Eam1105I EarI Eco47III Eco81I EcoO65I EcoRI EcoRV EcoT22I FbaI FokI FseI FspI HincII HindII HpaI KpnI Ksp632I MamI MfeI MflI MluI MluNI MroI MscI MseI MunI NcoI NdeI NruI NsiI NspI NspV PacI PmeI PshBI Psp1406I PvuI PvuII RcaI RsrII SacI SalI SapI SbfI SfiI SfuI SnaBI SpeI SphI Sse8387I SspBI SspI SwaI Tru9I Tsp509I TspRI XbaI XcmI XhoII XmnI ################################### # ONE CUT ENZYMES ( 42 enzymes) # ################################### AatII 945 GACGTC GACGT^C AflIII 1151 ACACGT A^C[GA][CT]GT AgeI 1328 ACCGGT A^CCGGT AscI 955 GGCGCGCC GG^CGCGCC AspI 849 GACCTCGTC GAC[ACGT]^[ACGT][ACGT]GTC BbrPI 667 CACGTG CAC^GTG BfaI 448 CTAG C^TAG BlpI 885 GCTGAGC GC^T[ACGT]AGC BpmI 787 CTGGAG CTGGAG(16/14) Bpu1102I 885 GCTGAGC GC^T[ACGT]AGC BsaAI 667 CACGTG [CT]AC^GT[GA] BsaWI 1328 ACCGGT [AT]^CCGG[AT] BsiWI 305 CGTACG C^GTACG BsmBI 947 CGTCTC CGTCTC(1/5) CelII 885 GCTGAGC GC^T[ACGT]AGC DdeI 886 CTGAG C^T[ACGT]AG EcoT14I 428 CCAAGG C^C[AT][AT]GG HindIII 718 AAGCTT A^AGCTT MaeI 448 CTAG C^TAG MaeIII 847 GTGAC ^GT[ACGT]AC NheI 447 GCTAGC G^CTAGC PaeR7I 1315 CTCGAG C^TCGAG PflFI 849 GACCTCGTC GAC[ACGT]^[ACGT][ACGT]GTC PflMI 980 CCAAGCTGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG PinAI 1328 ACCGGT A^CCGGT PmaCI 667 CACGTG CAC^GTG PmlI 667 CACGTG CAC^GTG PpuMI 737 GGGACCC [GA]G^G[AT]CC[CT] PshAI 601 GACGAGAGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC ScaI 200 AGTACT AGT^ACT SexAI 487 ACCTGGT A^CC[AT]GGT SfaNI 1363 GATGC GCATC(5/9) SgrAI 505 CGCCGGCG C[GA]^CCGG[CT]G StuI 389 AGGCCT AGG^CCT StyI 428 CCAAGG C^C[AT][AT]GG TaqI 1316 TCGA T^CGA TfiI 751 GAATC G^A[AT]TC Tsp45I 847 GTGAC ^GT[GC]AC Tth111I 849 GACCTCGTC GAC[ACGT]^[ACGT][ACGT]GTC TthHB8I 1316 TCGA T^CGA Van91I 980 CCAAGCTGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG XhoI 1315 CTCGAG C^TCGAG ################################### # TWO CUT ENZYMES ( 13 enzymes) # ################################### AlwI 656 GGATC GGATC(4/5) 684 GGATC ApaI 539 GGGCCC GGGCC^C 1035 GGGCCC AspHI 403 GAGCAC G[AT]GC[AT]^C 693 GTGCTC BseRI 100 GAGGAG GAGGAG(10/8) 811 GAGGAG BsiHKAI 403 GAGCAC G[AT]GC[AT]^C 693 GTGCTC BsrBI 1312 CCGCTC CCGCTC(-3/-3) 820 GAGCGG BssSI 54 CTCGTG CACGAG(-5/-1) 690 CTCGTG HphI 458 TCACC GGTGA(8/7) 473 TCACC MboII 438 GAAGA GAAGA(8/7) 559 GAAGA MslI 273 CACCGGCATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG 459 CACCAAGATG NotI 1066 GCGGCCGC GC^GGCCGC 1237 GCGGCCGC PstI 196 CTGCAG CTGCA^G 1204 CTGCAG SfcI 196 CTGCAG C^T[GA][CT]AG 1204 CTGCAG ######################################### # 3 OR MORE CUT ENZYMES ( 85 enzymes) # ######################################### AccII AciI AcyI AfaI AluI AvaI AvaII BanI BanII BbeI BbvI BcnI BglI BmyI BsaHI BsaI BsaJI BsgI BsiEI BsiYI BslI BsmAI BsmFI BsoBI Bsp1286I BsrFI BssHII BssKI BstNI BstUI Cac8I CfoI Cfr10I Cfr13I DpnII DraII DsaI EaeI EagI EclXI Eco52I EcoNI EcoO109I EcoRII FauI Fnu4HI HaeII HaeIII HapII HgaI HhaI Hin1I HinP1I HinfI HpaII ItaI KasI KspI MaeII MboI MnlI MspA1I MspI MvaI MvnI MwoI NaeI NarI NciI NdeII NgoMIV NlaIII NlaIV PleI RsaI SacII Sau3AI Sau96I ScrFI SfoI SmaI SmlI TseI XmaCI XmaI